Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis)
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572005000200010 http://hdl.handle.net/11449/68566 |
Resumo: | Caseins comprise make up about 80% of the total protein content of milk and present polymorphism with change in the amino acid sequence. Within this abundance of proteins, kappa-casein is noteworthy, since it has been associated with differences in milk yield, composition and processing. The objective of this study was to observe the existence of polymorphism in the kappa-casein gene in female buffaloes. For this purpose, blood samples from 115 female buffaloes, collected with vacutainer by needle punctionure of the jugular vein, were used. for genomic DNA extraction was done from blood samples. The PCR-RFLP and SSCP techniques demonstrated that the studied animals were monomorphic for the kappa-casein gene. Only allele B was observed in these animals, which was present in homozygosis. Therefore, it was not possible to quantify the gene action on milk yield and its constituents. The monomorphism observed in the population studied would allow the development of a method to identify mixtures of cow and buffalo milk in mozzarella cheese production, especially because, in cattle, the kappa-casein gene is polymorphic. Copyright by the Brazilian Society of Genetics. |
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Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis)BuffaloesPCR-RFLPPolymorphismSSCPgenomic DNAkappa caseinmolecular markeralleleanimal experimentanimal geneticsblood samplingbuffalocheesemakingcontrolled studyDNA extractionfemalegenetic polymorphismhomozygosityjugular veinmilk productionmilk yieldnonhumanpolymerase chain reactionrestriction fragment length polymorphismsingle strand conformation polymorphismAnimaliaBos taurusBubalusBubalus bubalisCaseins comprise make up about 80% of the total protein content of milk and present polymorphism with change in the amino acid sequence. Within this abundance of proteins, kappa-casein is noteworthy, since it has been associated with differences in milk yield, composition and processing. The objective of this study was to observe the existence of polymorphism in the kappa-casein gene in female buffaloes. For this purpose, blood samples from 115 female buffaloes, collected with vacutainer by needle punctionure of the jugular vein, were used. for genomic DNA extraction was done from blood samples. The PCR-RFLP and SSCP techniques demonstrated that the studied animals were monomorphic for the kappa-casein gene. Only allele B was observed in these animals, which was present in homozygosis. Therefore, it was not possible to quantify the gene action on milk yield and its constituents. The monomorphism observed in the population studied would allow the development of a method to identify mixtures of cow and buffalo milk in mozzarella cheese production, especially because, in cattle, the kappa-casein gene is polymorphic. Copyright by the Brazilian Society of Genetics.Universidade Estadual Paulista 'Júlio de Mesquita Filho' Faculdade de Ciências Agrárias e Veterinárias Departamento de Zootecnia, Via de Acesso P.D. Castellane s/n, 14884-900 Jaboticabal, SPUniversidade Estadual Paulista 'Júlio de Mesquita Filho' Faculdade de Ciências Agrárias e Veterinárias Departamento de Biologia, Via de Acesso P.D. Castellane s/n, 14884-900 Jaboticabal, SPUniversidad de Antioquia Facultad de Ciencias Agrarias, AntioquiaUniversidade Estadual Paulista 'Júlio de Mesquita Filho' Faculdade de Ciências Agrárias e Veterinárias Departamento de Zootecnia, Via de Acesso P.D. Castellane s/n, 14884-900 Jaboticabal, SPUniversidade Estadual Paulista 'Júlio de Mesquita Filho' Faculdade de Ciências Agrárias e Veterinárias Departamento de Biologia, Via de Acesso P.D. Castellane s/n, 14884-900 Jaboticabal, SPUniversidade Estadual Paulista (Unesp)Otaviano, Antonio Roberto [UNESP]Tonhati, Humberto [UNESP]Desidério Sena, Janete Aparecida [UNESP]Cerón Muñoz, Mario Fernando2014-05-27T11:21:43Z2014-05-27T11:21:43Z2005-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article237-241application/pdfhttp://dx.doi.org/10.1590/S1415-47572005000200010Genetics and Molecular Biology, v. 28, n. 2, p. 237-241, 2005.1415-47571678-4685http://hdl.handle.net/11449/6856610.1590/S1415-47572005000200010S1415-47572005000200010WOS:0002309292000102-s2.0-306444688652-s2.0-30644468865.pdf7445254960858159Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2024-06-07T18:44:15Zoai:repositorio.unesp.br:11449/68566Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:17:34.065420Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis) |
title |
Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis) |
spellingShingle |
Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis) Otaviano, Antonio Roberto [UNESP] Buffaloes PCR-RFLP Polymorphism SSCP genomic DNA kappa casein molecular marker allele animal experiment animal genetics blood sampling buffalo cheesemaking controlled study DNA extraction female genetic polymorphism homozygosity jugular vein milk production milk yield nonhuman polymerase chain reaction restriction fragment length polymorphism single strand conformation polymorphism Animalia Bos taurus Bubalus Bubalus bubalis |
title_short |
Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis) |
title_full |
Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis) |
title_fullStr |
Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis) |
title_full_unstemmed |
Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis) |
title_sort |
Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis) |
author |
Otaviano, Antonio Roberto [UNESP] |
author_facet |
Otaviano, Antonio Roberto [UNESP] Tonhati, Humberto [UNESP] Desidério Sena, Janete Aparecida [UNESP] Cerón Muñoz, Mario Fernando |
author_role |
author |
author2 |
Tonhati, Humberto [UNESP] Desidério Sena, Janete Aparecida [UNESP] Cerón Muñoz, Mario Fernando |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Otaviano, Antonio Roberto [UNESP] Tonhati, Humberto [UNESP] Desidério Sena, Janete Aparecida [UNESP] Cerón Muñoz, Mario Fernando |
dc.subject.por.fl_str_mv |
Buffaloes PCR-RFLP Polymorphism SSCP genomic DNA kappa casein molecular marker allele animal experiment animal genetics blood sampling buffalo cheesemaking controlled study DNA extraction female genetic polymorphism homozygosity jugular vein milk production milk yield nonhuman polymerase chain reaction restriction fragment length polymorphism single strand conformation polymorphism Animalia Bos taurus Bubalus Bubalus bubalis |
topic |
Buffaloes PCR-RFLP Polymorphism SSCP genomic DNA kappa casein molecular marker allele animal experiment animal genetics blood sampling buffalo cheesemaking controlled study DNA extraction female genetic polymorphism homozygosity jugular vein milk production milk yield nonhuman polymerase chain reaction restriction fragment length polymorphism single strand conformation polymorphism Animalia Bos taurus Bubalus Bubalus bubalis |
description |
Caseins comprise make up about 80% of the total protein content of milk and present polymorphism with change in the amino acid sequence. Within this abundance of proteins, kappa-casein is noteworthy, since it has been associated with differences in milk yield, composition and processing. The objective of this study was to observe the existence of polymorphism in the kappa-casein gene in female buffaloes. For this purpose, blood samples from 115 female buffaloes, collected with vacutainer by needle punctionure of the jugular vein, were used. for genomic DNA extraction was done from blood samples. The PCR-RFLP and SSCP techniques demonstrated that the studied animals were monomorphic for the kappa-casein gene. Only allele B was observed in these animals, which was present in homozygosis. Therefore, it was not possible to quantify the gene action on milk yield and its constituents. The monomorphism observed in the population studied would allow the development of a method to identify mixtures of cow and buffalo milk in mozzarella cheese production, especially because, in cattle, the kappa-casein gene is polymorphic. Copyright by the Brazilian Society of Genetics. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-12-01 2014-05-27T11:21:43Z 2014-05-27T11:21:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572005000200010 Genetics and Molecular Biology, v. 28, n. 2, p. 237-241, 2005. 1415-4757 1678-4685 http://hdl.handle.net/11449/68566 10.1590/S1415-47572005000200010 S1415-47572005000200010 WOS:000230929200010 2-s2.0-30644468865 2-s2.0-30644468865.pdf 7445254960858159 |
url |
http://dx.doi.org/10.1590/S1415-47572005000200010 http://hdl.handle.net/11449/68566 |
identifier_str_mv |
Genetics and Molecular Biology, v. 28, n. 2, p. 237-241, 2005. 1415-4757 1678-4685 10.1590/S1415-47572005000200010 S1415-47572005000200010 WOS:000230929200010 2-s2.0-30644468865 2-s2.0-30644468865.pdf 7445254960858159 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
237-241 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129414560481280 |