Virucidal activity of the antiseptic mouthwash and dental gel containing anionic phthalocyanine derivative: In vitro study

• Autor(a) principal: Santos, Caique
• Data de Publicação: 2021
• Outros Autores: Orcina, Bernardo da Fonseca, Reia, Verônica Caroline Brito, Ribeiro, Lucas Gabriel [UNESP], Grotto, Rejane M. T. [UNESP], Prudenciatti, Aruã [UNESP], de Moraes, Leonardo Nazario [UNESP], Zangrando, Mariana Ragghianti, Vilhena, Fabiano Vieira, Santos, Paulo Sérgio da Silva
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.2147/CCIDE.S315419; http://hdl.handle.net/11449/221913
• topic: Coronavirus infections; Dentifrices; In vitro techniques; Mouthwash

Aim: This research suggested an in vitro virucidal action of a dental gel and a mouthwash with phthalocyanine derivative. Purpose: The aim of this study was to report an in vitro study evaluating the virucidal capacity of mouthwash and dental gel containing anionic phthalocyanine derivate (APD). Methods: The research followed the recommendations of the National Health Surveillance Agency (ANVISA) and adapted methodology, described in the standards EN14776: 2015; ASTM E1053-11 and the Robert Koch Institute – RKI, in addition to good laboratory practices (GLP). The determination of the percentage of inactivation of the SARS-CoV-2 virus particles was carried out by imposing the viral solution in contact with the respective tested products, with intervals of 30 seconds, 1 and 5 minutes, with subsequent submission of the aliquots, recovered in cell culture microplates following virus titration using the TCID50 (50% Median Tissue Culture Infectious Dose). Results: The Mouthwash APD presented 90% of viral inactivation percentage, while the dental gel APD demonstrated 99.99% of viral inactivation. Conclusion: In vitro analyses showed that mouthwash and dental gel APD can reduce the viability of SARS-CoV-2 virus particles.