Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/pathogens11111302 http://hdl.handle.net/11449/249366 |
Resumo: | Under laboratory conditions, Triatoma rosai and T. sordida are able to cross and produce hybrids. In the face of climate and environmental changes, the study of hybrids of triatomines has evolutionary and epidemiological implications. Therefore, we performed morphological, cytological and molecular studies and characterized the feeding and defecation pattern of hybrids from crosses between T. sordida and T. rosai. The morphological characterization of the female genitalia of the hybrids showed that characteristics of both parental species segregated in the hybrids. Cytogenetic analyzes of hybrids showed regular metaphases. According to molecular studies, the mitochondrial marker Cytochrome B (CytB) related the hybrids with T. sordida and the nuclear marker Internal Transcribed Spacer 1 (ITS-1) related the hybrids with T. rosai. Both parents and hybrids defecated during the blood meal. Thus, the hybrids resulting from the cross between T. sordida and T. rosai presented segregation of phenotypic characters of both parental species, 100% homeology between homeologous chromosomes, phylogenetic relationship with T sordida and with T. rosai (with CytB and ITS-1, respectively), and, finally, feeding and defecation patterns similar to the parents. |
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Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)Chagas disease vectorscytogeneticshybridizationmolecular biologymorphologyUnder laboratory conditions, Triatoma rosai and T. sordida are able to cross and produce hybrids. In the face of climate and environmental changes, the study of hybrids of triatomines has evolutionary and epidemiological implications. Therefore, we performed morphological, cytological and molecular studies and characterized the feeding and defecation pattern of hybrids from crosses between T. sordida and T. rosai. The morphological characterization of the female genitalia of the hybrids showed that characteristics of both parental species segregated in the hybrids. Cytogenetic analyzes of hybrids showed regular metaphases. According to molecular studies, the mitochondrial marker Cytochrome B (CytB) related the hybrids with T. sordida and the nuclear marker Internal Transcribed Spacer 1 (ITS-1) related the hybrids with T. rosai. Both parents and hybrids defecated during the blood meal. Thus, the hybrids resulting from the cross between T. sordida and T. rosai presented segregation of phenotypic characters of both parental species, 100% homeology between homeologous chromosomes, phylogenetic relationship with T sordida and with T. rosai (with CytB and ITS-1, respectively), and, finally, feeding and defecation patterns similar to the parents.Instituto de Biociências de Botucatu Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SPInstituto de Biociências Letras e Ciêcias Exatas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SPLaboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos Instituto Oswaldo Cruz (FIOCRUZ), RJLaboratório de Parasitologia Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SPLaboratório de Entomologia em Saúde Pública Faculdade de Saúde Pública Universidade de São Paulo (USP), SPInstituto de Biociências de Botucatu Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SPInstituto de Biociências Letras e Ciêcias Exatas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SPLaboratório de Parasitologia Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SPUniversidade Estadual Paulista (UNESP)Instituto Oswaldo Cruz (FIOCRUZ)Universidade de São Paulo (USP)Vicente, Roberto Dezan [UNESP]Madeira, Fernanda Fernandez [UNESP]Borsatto, Kelly Cristine [UNESP]Garcia, Ariane Cristina Caris [UNESP]Cristal, Daniel Cesaretto [UNESP]Delgado, Luiza Maria Grzyb [UNESP]Bittinelli, Isadora de Freitas [UNESP]De Mello, Denis Vinicius [UNESP]Dos Reis, Yago Visinho [UNESP]Ravazi, Amanda [UNESP]Galvão, CleberDe Azeredo-Oliveira, Maria Tercília Vilela [UNESP]Da Rosa, João Aristeu [UNESP]De Oliveira, JaderAlevi, Kaio Cesar Chaboli [UNESP]2023-07-29T15:14:08Z2023-07-29T15:14:08Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pathogens11111302Pathogens, v. 11, n. 11, 2022.2076-0817http://hdl.handle.net/11449/24936610.3390/pathogens111113022-s2.0-85141792627Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogensinfo:eu-repo/semantics/openAccess2023-07-29T15:14:08Zoai:repositorio.unesp.br:11449/249366Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T15:14:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae) |
title |
Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae) |
spellingShingle |
Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae) Vicente, Roberto Dezan [UNESP] Chagas disease vectors cytogenetics hybridization molecular biology morphology |
title_short |
Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae) |
title_full |
Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae) |
title_fullStr |
Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae) |
title_full_unstemmed |
Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae) |
title_sort |
Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae) |
author |
Vicente, Roberto Dezan [UNESP] |
author_facet |
Vicente, Roberto Dezan [UNESP] Madeira, Fernanda Fernandez [UNESP] Borsatto, Kelly Cristine [UNESP] Garcia, Ariane Cristina Caris [UNESP] Cristal, Daniel Cesaretto [UNESP] Delgado, Luiza Maria Grzyb [UNESP] Bittinelli, Isadora de Freitas [UNESP] De Mello, Denis Vinicius [UNESP] Dos Reis, Yago Visinho [UNESP] Ravazi, Amanda [UNESP] Galvão, Cleber De Azeredo-Oliveira, Maria Tercília Vilela [UNESP] Da Rosa, João Aristeu [UNESP] De Oliveira, Jader Alevi, Kaio Cesar Chaboli [UNESP] |
author_role |
author |
author2 |
Madeira, Fernanda Fernandez [UNESP] Borsatto, Kelly Cristine [UNESP] Garcia, Ariane Cristina Caris [UNESP] Cristal, Daniel Cesaretto [UNESP] Delgado, Luiza Maria Grzyb [UNESP] Bittinelli, Isadora de Freitas [UNESP] De Mello, Denis Vinicius [UNESP] Dos Reis, Yago Visinho [UNESP] Ravazi, Amanda [UNESP] Galvão, Cleber De Azeredo-Oliveira, Maria Tercília Vilela [UNESP] Da Rosa, João Aristeu [UNESP] De Oliveira, Jader Alevi, Kaio Cesar Chaboli [UNESP] |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Instituto Oswaldo Cruz (FIOCRUZ) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Vicente, Roberto Dezan [UNESP] Madeira, Fernanda Fernandez [UNESP] Borsatto, Kelly Cristine [UNESP] Garcia, Ariane Cristina Caris [UNESP] Cristal, Daniel Cesaretto [UNESP] Delgado, Luiza Maria Grzyb [UNESP] Bittinelli, Isadora de Freitas [UNESP] De Mello, Denis Vinicius [UNESP] Dos Reis, Yago Visinho [UNESP] Ravazi, Amanda [UNESP] Galvão, Cleber De Azeredo-Oliveira, Maria Tercília Vilela [UNESP] Da Rosa, João Aristeu [UNESP] De Oliveira, Jader Alevi, Kaio Cesar Chaboli [UNESP] |
dc.subject.por.fl_str_mv |
Chagas disease vectors cytogenetics hybridization molecular biology morphology |
topic |
Chagas disease vectors cytogenetics hybridization molecular biology morphology |
description |
Under laboratory conditions, Triatoma rosai and T. sordida are able to cross and produce hybrids. In the face of climate and environmental changes, the study of hybrids of triatomines has evolutionary and epidemiological implications. Therefore, we performed morphological, cytological and molecular studies and characterized the feeding and defecation pattern of hybrids from crosses between T. sordida and T. rosai. The morphological characterization of the female genitalia of the hybrids showed that characteristics of both parental species segregated in the hybrids. Cytogenetic analyzes of hybrids showed regular metaphases. According to molecular studies, the mitochondrial marker Cytochrome B (CytB) related the hybrids with T. sordida and the nuclear marker Internal Transcribed Spacer 1 (ITS-1) related the hybrids with T. rosai. Both parents and hybrids defecated during the blood meal. Thus, the hybrids resulting from the cross between T. sordida and T. rosai presented segregation of phenotypic characters of both parental species, 100% homeology between homeologous chromosomes, phylogenetic relationship with T sordida and with T. rosai (with CytB and ITS-1, respectively), and, finally, feeding and defecation patterns similar to the parents. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 2023-07-29T15:14:08Z 2023-07-29T15:14:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/pathogens11111302 Pathogens, v. 11, n. 11, 2022. 2076-0817 http://hdl.handle.net/11449/249366 10.3390/pathogens11111302 2-s2.0-85141792627 |
url |
http://dx.doi.org/10.3390/pathogens11111302 http://hdl.handle.net/11449/249366 |
identifier_str_mv |
Pathogens, v. 11, n. 11, 2022. 2076-0817 10.3390/pathogens11111302 2-s2.0-85141792627 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pathogens |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964609369604096 |