Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)

Detalhes bibliográficos
Autor(a) principal: Vicente, Roberto Dezan [UNESP]
Data de Publicação: 2022
Outros Autores: Madeira, Fernanda Fernandez [UNESP], Borsatto, Kelly Cristine [UNESP], Garcia, Ariane Cristina Caris [UNESP], Cristal, Daniel Cesaretto [UNESP], Delgado, Luiza Maria Grzyb [UNESP], Bittinelli, Isadora de Freitas [UNESP], De Mello, Denis Vinicius [UNESP], Dos Reis, Yago Visinho [UNESP], Ravazi, Amanda [UNESP], Galvão, Cleber, De Azeredo-Oliveira, Maria Tercília Vilela [UNESP], Da Rosa, João Aristeu [UNESP], De Oliveira, Jader, Alevi, Kaio Cesar Chaboli [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/pathogens11111302
http://hdl.handle.net/11449/249366
Resumo: Under laboratory conditions, Triatoma rosai and T. sordida are able to cross and produce hybrids. In the face of climate and environmental changes, the study of hybrids of triatomines has evolutionary and epidemiological implications. Therefore, we performed morphological, cytological and molecular studies and characterized the feeding and defecation pattern of hybrids from crosses between T. sordida and T. rosai. The morphological characterization of the female genitalia of the hybrids showed that characteristics of both parental species segregated in the hybrids. Cytogenetic analyzes of hybrids showed regular metaphases. According to molecular studies, the mitochondrial marker Cytochrome B (CytB) related the hybrids with T. sordida and the nuclear marker Internal Transcribed Spacer 1 (ITS-1) related the hybrids with T. rosai. Both parents and hybrids defecated during the blood meal. Thus, the hybrids resulting from the cross between T. sordida and T. rosai presented segregation of phenotypic characters of both parental species, 100% homeology between homeologous chromosomes, phylogenetic relationship with T sordida and with T. rosai (with CytB and ITS-1, respectively), and, finally, feeding and defecation patterns similar to the parents.
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spelling Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)Chagas disease vectorscytogeneticshybridizationmolecular biologymorphologyUnder laboratory conditions, Triatoma rosai and T. sordida are able to cross and produce hybrids. In the face of climate and environmental changes, the study of hybrids of triatomines has evolutionary and epidemiological implications. Therefore, we performed morphological, cytological and molecular studies and characterized the feeding and defecation pattern of hybrids from crosses between T. sordida and T. rosai. The morphological characterization of the female genitalia of the hybrids showed that characteristics of both parental species segregated in the hybrids. Cytogenetic analyzes of hybrids showed regular metaphases. According to molecular studies, the mitochondrial marker Cytochrome B (CytB) related the hybrids with T. sordida and the nuclear marker Internal Transcribed Spacer 1 (ITS-1) related the hybrids with T. rosai. Both parents and hybrids defecated during the blood meal. Thus, the hybrids resulting from the cross between T. sordida and T. rosai presented segregation of phenotypic characters of both parental species, 100% homeology between homeologous chromosomes, phylogenetic relationship with T sordida and with T. rosai (with CytB and ITS-1, respectively), and, finally, feeding and defecation patterns similar to the parents.Instituto de Biociências de Botucatu Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SPInstituto de Biociências Letras e Ciêcias Exatas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SPLaboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos Instituto Oswaldo Cruz (FIOCRUZ), RJLaboratório de Parasitologia Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SPLaboratório de Entomologia em Saúde Pública Faculdade de Saúde Pública Universidade de São Paulo (USP), SPInstituto de Biociências de Botucatu Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SPInstituto de Biociências Letras e Ciêcias Exatas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SPLaboratório de Parasitologia Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), SPUniversidade Estadual Paulista (UNESP)Instituto Oswaldo Cruz (FIOCRUZ)Universidade de São Paulo (USP)Vicente, Roberto Dezan [UNESP]Madeira, Fernanda Fernandez [UNESP]Borsatto, Kelly Cristine [UNESP]Garcia, Ariane Cristina Caris [UNESP]Cristal, Daniel Cesaretto [UNESP]Delgado, Luiza Maria Grzyb [UNESP]Bittinelli, Isadora de Freitas [UNESP]De Mello, Denis Vinicius [UNESP]Dos Reis, Yago Visinho [UNESP]Ravazi, Amanda [UNESP]Galvão, CleberDe Azeredo-Oliveira, Maria Tercília Vilela [UNESP]Da Rosa, João Aristeu [UNESP]De Oliveira, JaderAlevi, Kaio Cesar Chaboli [UNESP]2023-07-29T15:14:08Z2023-07-29T15:14:08Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pathogens11111302Pathogens, v. 11, n. 11, 2022.2076-0817http://hdl.handle.net/11449/24936610.3390/pathogens111113022-s2.0-85141792627Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogensinfo:eu-repo/semantics/openAccess2023-07-29T15:14:08Zoai:repositorio.unesp.br:11449/249366Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T15:14:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)
title Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)
spellingShingle Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)
Vicente, Roberto Dezan [UNESP]
Chagas disease vectors
cytogenetics
hybridization
molecular biology
morphology
title_short Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)
title_full Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)
title_fullStr Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)
title_full_unstemmed Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)
title_sort Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)
author Vicente, Roberto Dezan [UNESP]
author_facet Vicente, Roberto Dezan [UNESP]
Madeira, Fernanda Fernandez [UNESP]
Borsatto, Kelly Cristine [UNESP]
Garcia, Ariane Cristina Caris [UNESP]
Cristal, Daniel Cesaretto [UNESP]
Delgado, Luiza Maria Grzyb [UNESP]
Bittinelli, Isadora de Freitas [UNESP]
De Mello, Denis Vinicius [UNESP]
Dos Reis, Yago Visinho [UNESP]
Ravazi, Amanda [UNESP]
Galvão, Cleber
De Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
Da Rosa, João Aristeu [UNESP]
De Oliveira, Jader
Alevi, Kaio Cesar Chaboli [UNESP]
author_role author
author2 Madeira, Fernanda Fernandez [UNESP]
Borsatto, Kelly Cristine [UNESP]
Garcia, Ariane Cristina Caris [UNESP]
Cristal, Daniel Cesaretto [UNESP]
Delgado, Luiza Maria Grzyb [UNESP]
Bittinelli, Isadora de Freitas [UNESP]
De Mello, Denis Vinicius [UNESP]
Dos Reis, Yago Visinho [UNESP]
Ravazi, Amanda [UNESP]
Galvão, Cleber
De Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
Da Rosa, João Aristeu [UNESP]
De Oliveira, Jader
Alevi, Kaio Cesar Chaboli [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Instituto Oswaldo Cruz (FIOCRUZ)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Vicente, Roberto Dezan [UNESP]
Madeira, Fernanda Fernandez [UNESP]
Borsatto, Kelly Cristine [UNESP]
Garcia, Ariane Cristina Caris [UNESP]
Cristal, Daniel Cesaretto [UNESP]
Delgado, Luiza Maria Grzyb [UNESP]
Bittinelli, Isadora de Freitas [UNESP]
De Mello, Denis Vinicius [UNESP]
Dos Reis, Yago Visinho [UNESP]
Ravazi, Amanda [UNESP]
Galvão, Cleber
De Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
Da Rosa, João Aristeu [UNESP]
De Oliveira, Jader
Alevi, Kaio Cesar Chaboli [UNESP]
dc.subject.por.fl_str_mv Chagas disease vectors
cytogenetics
hybridization
molecular biology
morphology
topic Chagas disease vectors
cytogenetics
hybridization
molecular biology
morphology
description Under laboratory conditions, Triatoma rosai and T. sordida are able to cross and produce hybrids. In the face of climate and environmental changes, the study of hybrids of triatomines has evolutionary and epidemiological implications. Therefore, we performed morphological, cytological and molecular studies and characterized the feeding and defecation pattern of hybrids from crosses between T. sordida and T. rosai. The morphological characterization of the female genitalia of the hybrids showed that characteristics of both parental species segregated in the hybrids. Cytogenetic analyzes of hybrids showed regular metaphases. According to molecular studies, the mitochondrial marker Cytochrome B (CytB) related the hybrids with T. sordida and the nuclear marker Internal Transcribed Spacer 1 (ITS-1) related the hybrids with T. rosai. Both parents and hybrids defecated during the blood meal. Thus, the hybrids resulting from the cross between T. sordida and T. rosai presented segregation of phenotypic characters of both parental species, 100% homeology between homeologous chromosomes, phylogenetic relationship with T sordida and with T. rosai (with CytB and ITS-1, respectively), and, finally, feeding and defecation patterns similar to the parents.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-01
2023-07-29T15:14:08Z
2023-07-29T15:14:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/pathogens11111302
Pathogens, v. 11, n. 11, 2022.
2076-0817
http://hdl.handle.net/11449/249366
10.3390/pathogens11111302
2-s2.0-85141792627
url http://dx.doi.org/10.3390/pathogens11111302
http://hdl.handle.net/11449/249366
identifier_str_mv Pathogens, v. 11, n. 11, 2022.
2076-0817
10.3390/pathogens11111302
2-s2.0-85141792627
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pathogens
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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