Orange Juice and Yogurt Carrying Probiotic Bacillus coagulans GBI-30 6086: Impact of Intake on Wistar Male Rats Health Parameters and Gut Bacterial Diversity
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fmicb.2021.623951 http://hdl.handle.net/11449/206481 |
Resumo: | This study aimed to investigate the impact of the food matrix (orange juice and yogurt) on the effects of the spore-forming probiotic microorganism Bacillus coagulans GBI-30 6086 in health parameters and gastrointestinal tract (gut) bacterial diversity in Wistar male rats. Rats (n = 48) were randomly distributed into six groups. The groups were the Control (which received sterile distilled water), Juice (which received orange juice), Yogurt (which received yogurt), Probiotic Bacillus (which received B. coagulans GBI-30 6086 in distilled water), Probiotic Juice (which received orange juice with B. coagulans GBI-30 6086), and Probiotic Yogurt (which received yogurt with B. coagulans GBI-30 6086). Each animal belonging to the different groups was treated for 21 days. The daily administration of probiotic juice or probiotic yogurt did not affect the rats’ food or body weight. Rats fed with Probiotic Yogurt showed lower glucose and triglycerides levels (p < 0.05) in comparison to the control group (p < 0.05), while no changes in these parameters were observed in the rats fed with Probiotic Juice. Rats fed with Probiotic Yogurt showed a higher gut bacterial diversity than the control group (p < 0.05), and higher abundance (p < 0.05) of Vibrionales, Enterobacteriales, Burkholderiales, Erysipelotrichales, and Bifidobacteriales compared to all other groups. No changes were observed in the expression levels of antioxidant enzymes or heat shock protein 70 of rats fed with probiotic yogurt or probiotic juice. Results reveal that the consumption of yogurt containing B. coagulans GBI-30 6086 decreases triglycerides and glucose levels and positively impacts the gut bacterial ecology in healthy rats. These animal model findings indicate that the matrix also impacts the functionality of foods carrying spore-forming probiotics. Besides, this research indicates that yogurt is also a suitable food carrier of Bacillus coagulans GBI-30 6086. |
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Orange Juice and Yogurt Carrying Probiotic Bacillus coagulans GBI-30 6086: Impact of Intake on Wistar Male Rats Health Parameters and Gut Bacterial Diversitybeneficial microbesfermented foodfunctional foodintestinal microbiomespore-forming bacteriaThis study aimed to investigate the impact of the food matrix (orange juice and yogurt) on the effects of the spore-forming probiotic microorganism Bacillus coagulans GBI-30 6086 in health parameters and gastrointestinal tract (gut) bacterial diversity in Wistar male rats. Rats (n = 48) were randomly distributed into six groups. The groups were the Control (which received sterile distilled water), Juice (which received orange juice), Yogurt (which received yogurt), Probiotic Bacillus (which received B. coagulans GBI-30 6086 in distilled water), Probiotic Juice (which received orange juice with B. coagulans GBI-30 6086), and Probiotic Yogurt (which received yogurt with B. coagulans GBI-30 6086). Each animal belonging to the different groups was treated for 21 days. The daily administration of probiotic juice or probiotic yogurt did not affect the rats’ food or body weight. Rats fed with Probiotic Yogurt showed lower glucose and triglycerides levels (p < 0.05) in comparison to the control group (p < 0.05), while no changes in these parameters were observed in the rats fed with Probiotic Juice. Rats fed with Probiotic Yogurt showed a higher gut bacterial diversity than the control group (p < 0.05), and higher abundance (p < 0.05) of Vibrionales, Enterobacteriales, Burkholderiales, Erysipelotrichales, and Bifidobacteriales compared to all other groups. No changes were observed in the expression levels of antioxidant enzymes or heat shock protein 70 of rats fed with probiotic yogurt or probiotic juice. Results reveal that the consumption of yogurt containing B. coagulans GBI-30 6086 decreases triglycerides and glucose levels and positively impacts the gut bacterial ecology in healthy rats. These animal model findings indicate that the matrix also impacts the functionality of foods carrying spore-forming probiotics. Besides, this research indicates that yogurt is also a suitable food carrier of Bacillus coagulans GBI-30 6086.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Food Science and Nutrition Faculty of Food Engineering University of CampinasInstitute of Biosciences Department of General and Applied Biology São Paulo State University (UNESP)Laboratory of Microbial Processes in Food Department of Food Engineering Technology Center Federal University of ParaíbaDepartment of Physical Education Federal University of Great DouradosResearch Informatics Core Research Resource Center University of Illinois at ChicagoMicrobial Resources Division Research Center for Chemistry Biology and Agriculture (CPQBA) University of CampinasInternal Medicine Department Faculty of Medical Sciences University of CampinasInstitute of Biosciences Department of General and Applied Biology São Paulo State University (UNESP)CAPES: 001CNPq: 302763/2014-7CNPq: 305804/2017-0Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Federal University of ParaíbaFederal University of Great DouradosUniversity of Illinois at ChicagoAlmada-Érix, Carine N.Almada, Caroline N.Cabral, Lucélia [UNESP]Barros de Medeiros, Viviane PriscilaRoquetto, Aline R.Santos-Junior, Valfredo A.Fontes, MellineGonçalves, Any Elisa S. S.dos Santos, AndreyLollo, Pablo C.Magnani, MarcianeSant’Ana, Anderson S.2021-06-25T10:32:45Z2021-06-25T10:32:45Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fmicb.2021.623951Frontiers in Microbiology, v. 12.1664-302Xhttp://hdl.handle.net/11449/20648110.3389/fmicb.2021.6239512-s2.0-85107883857Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T06:45:02Zoai:repositorio.unesp.br:11449/206481Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:53:52.698158Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Orange Juice and Yogurt Carrying Probiotic Bacillus coagulans GBI-30 6086: Impact of Intake on Wistar Male Rats Health Parameters and Gut Bacterial Diversity |
title |
Orange Juice and Yogurt Carrying Probiotic Bacillus coagulans GBI-30 6086: Impact of Intake on Wistar Male Rats Health Parameters and Gut Bacterial Diversity |
spellingShingle |
Orange Juice and Yogurt Carrying Probiotic Bacillus coagulans GBI-30 6086: Impact of Intake on Wistar Male Rats Health Parameters and Gut Bacterial Diversity Almada-Érix, Carine N. beneficial microbes fermented food functional food intestinal microbiome spore-forming bacteria |
title_short |
Orange Juice and Yogurt Carrying Probiotic Bacillus coagulans GBI-30 6086: Impact of Intake on Wistar Male Rats Health Parameters and Gut Bacterial Diversity |
title_full |
Orange Juice and Yogurt Carrying Probiotic Bacillus coagulans GBI-30 6086: Impact of Intake on Wistar Male Rats Health Parameters and Gut Bacterial Diversity |
title_fullStr |
Orange Juice and Yogurt Carrying Probiotic Bacillus coagulans GBI-30 6086: Impact of Intake on Wistar Male Rats Health Parameters and Gut Bacterial Diversity |
title_full_unstemmed |
Orange Juice and Yogurt Carrying Probiotic Bacillus coagulans GBI-30 6086: Impact of Intake on Wistar Male Rats Health Parameters and Gut Bacterial Diversity |
title_sort |
Orange Juice and Yogurt Carrying Probiotic Bacillus coagulans GBI-30 6086: Impact of Intake on Wistar Male Rats Health Parameters and Gut Bacterial Diversity |
author |
Almada-Érix, Carine N. |
author_facet |
Almada-Érix, Carine N. Almada, Caroline N. Cabral, Lucélia [UNESP] Barros de Medeiros, Viviane Priscila Roquetto, Aline R. Santos-Junior, Valfredo A. Fontes, Melline Gonçalves, Any Elisa S. S. dos Santos, Andrey Lollo, Pablo C. Magnani, Marciane Sant’Ana, Anderson S. |
author_role |
author |
author2 |
Almada, Caroline N. Cabral, Lucélia [UNESP] Barros de Medeiros, Viviane Priscila Roquetto, Aline R. Santos-Junior, Valfredo A. Fontes, Melline Gonçalves, Any Elisa S. S. dos Santos, Andrey Lollo, Pablo C. Magnani, Marciane Sant’Ana, Anderson S. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Federal University of Paraíba Federal University of Great Dourados University of Illinois at Chicago |
dc.contributor.author.fl_str_mv |
Almada-Érix, Carine N. Almada, Caroline N. Cabral, Lucélia [UNESP] Barros de Medeiros, Viviane Priscila Roquetto, Aline R. Santos-Junior, Valfredo A. Fontes, Melline Gonçalves, Any Elisa S. S. dos Santos, Andrey Lollo, Pablo C. Magnani, Marciane Sant’Ana, Anderson S. |
dc.subject.por.fl_str_mv |
beneficial microbes fermented food functional food intestinal microbiome spore-forming bacteria |
topic |
beneficial microbes fermented food functional food intestinal microbiome spore-forming bacteria |
description |
This study aimed to investigate the impact of the food matrix (orange juice and yogurt) on the effects of the spore-forming probiotic microorganism Bacillus coagulans GBI-30 6086 in health parameters and gastrointestinal tract (gut) bacterial diversity in Wistar male rats. Rats (n = 48) were randomly distributed into six groups. The groups were the Control (which received sterile distilled water), Juice (which received orange juice), Yogurt (which received yogurt), Probiotic Bacillus (which received B. coagulans GBI-30 6086 in distilled water), Probiotic Juice (which received orange juice with B. coagulans GBI-30 6086), and Probiotic Yogurt (which received yogurt with B. coagulans GBI-30 6086). Each animal belonging to the different groups was treated for 21 days. The daily administration of probiotic juice or probiotic yogurt did not affect the rats’ food or body weight. Rats fed with Probiotic Yogurt showed lower glucose and triglycerides levels (p < 0.05) in comparison to the control group (p < 0.05), while no changes in these parameters were observed in the rats fed with Probiotic Juice. Rats fed with Probiotic Yogurt showed a higher gut bacterial diversity than the control group (p < 0.05), and higher abundance (p < 0.05) of Vibrionales, Enterobacteriales, Burkholderiales, Erysipelotrichales, and Bifidobacteriales compared to all other groups. No changes were observed in the expression levels of antioxidant enzymes or heat shock protein 70 of rats fed with probiotic yogurt or probiotic juice. Results reveal that the consumption of yogurt containing B. coagulans GBI-30 6086 decreases triglycerides and glucose levels and positively impacts the gut bacterial ecology in healthy rats. These animal model findings indicate that the matrix also impacts the functionality of foods carrying spore-forming probiotics. Besides, this research indicates that yogurt is also a suitable food carrier of Bacillus coagulans GBI-30 6086. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:32:45Z 2021-06-25T10:32:45Z 2021-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2021.623951 Frontiers in Microbiology, v. 12. 1664-302X http://hdl.handle.net/11449/206481 10.3389/fmicb.2021.623951 2-s2.0-85107883857 |
url |
http://dx.doi.org/10.3389/fmicb.2021.623951 http://hdl.handle.net/11449/206481 |
identifier_str_mv |
Frontiers in Microbiology, v. 12. 1664-302X 10.3389/fmicb.2021.623951 2-s2.0-85107883857 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128718504198144 |