Yield and Phytochemical Characterization of Essential Oil from Ocimum selloi B. Obtained by Hydrodistillation and Supercritical Fluid Extraction

Detalhes bibliográficos
Autor(a) principal: Aparecida Salgado de Morais, L.
Data de Publicação: 2011
Outros Autores: Ming, Lin Chau [UNESP], Ortiz Mayo Marques, M., Almeida Meireles, M. A., Mathe, A., Barata, A. M., Carlen, C., Craker, L.
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/196029
Resumo: The yield and chemical composition of essential oils from leaves of Ocimum selloi B. submitted to organic and mineral fertilization, obtained by hydrodistillation and supercritical fluid extraction (SFE) were compared. Essential oil was extracted in a Clevenger-type apparatus for 2 h 30 min and analyzed by GC-MS (Shimadzu, QP 5050-DB-5 capillary column - 30 m x 0.25 mm x 0.25 mu m). Carrier gas was helium (1.7 ml/min); split ratio: 1:30. Temperature program: 50 degrees C, rising to 180 degrees C at 5 degrees C/min, 180 degrees C, rising to 280 degrees C at 10 degrees C/min. Injector temperature: 240 degrees C and detector temperature: 230 degrees C. Identifications of chemical compounds were made by matching their mass spectra and Kovat's indices (IK) values with known compounds reported in the literature. An Applied Separations-apparatus (Speed SFE, model 7071, Allentown, PA, EUA) was used for SFE extractions. They were conducted at pressure 200 bar and temperature 30 degrees C (20 min in static mode and 40 min in dynamic mode). The supercritical CO2 flow rate was (6.8 +/- 0.7) x 10-5 kg-CO2/s. The essential oil collected was immersed in ethylene glycol bath (5 degrees C). The yield of essential oils obtained by SFE was larger than hydrodistillation in both fertilization treatments (279 and 333% for organic and mineral fertilizations, respectively). There were no differences between the fertilization treatments. The amount of the volatile components showed by GC-MS chromatogram was highest in the essential oil obtained by hydrodistillation than SFE. The main volatile constituents of the essential oils were trans-anethole (Hydrodistillation: organic - 52.4%; mineral - 55.0%/ SFE: Hydrodistillation - 62.8%; mineral - 66.8%) and methyl-chavicol (Hydrodistillation: organic - 37.3%; mineral - 38.3%/SFE: organic - 8.4%; mineral - 4.3%). A reduction of methyl-chavicol relative proportion of essential oil obtained by SFE was observed. Cys-anethole, alpha-copaene, trans-cariofilene, germacrene-D, beta-selinene, biciclogermacrene and spathulenol were expressed only in hydrodistillation. The extraction of essential oil by SFE presented larger yield of essential oil than hydrodistillation technique, presenting, however, these essential oils, different phytochemical profiles.
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spelling Yield and Phytochemical Characterization of Essential Oil from Ocimum selloi B. Obtained by Hydrodistillation and Supercritical Fluid Extractionmethyl-chavicoltrans-anetholeSFEorganic treatmentmineral treatmentThe yield and chemical composition of essential oils from leaves of Ocimum selloi B. submitted to organic and mineral fertilization, obtained by hydrodistillation and supercritical fluid extraction (SFE) were compared. Essential oil was extracted in a Clevenger-type apparatus for 2 h 30 min and analyzed by GC-MS (Shimadzu, QP 5050-DB-5 capillary column - 30 m x 0.25 mm x 0.25 mu m). Carrier gas was helium (1.7 ml/min); split ratio: 1:30. Temperature program: 50 degrees C, rising to 180 degrees C at 5 degrees C/min, 180 degrees C, rising to 280 degrees C at 10 degrees C/min. Injector temperature: 240 degrees C and detector temperature: 230 degrees C. Identifications of chemical compounds were made by matching their mass spectra and Kovat's indices (IK) values with known compounds reported in the literature. An Applied Separations-apparatus (Speed SFE, model 7071, Allentown, PA, EUA) was used for SFE extractions. They were conducted at pressure 200 bar and temperature 30 degrees C (20 min in static mode and 40 min in dynamic mode). The supercritical CO2 flow rate was (6.8 +/- 0.7) x 10-5 kg-CO2/s. The essential oil collected was immersed in ethylene glycol bath (5 degrees C). The yield of essential oils obtained by SFE was larger than hydrodistillation in both fertilization treatments (279 and 333% for organic and mineral fertilizations, respectively). There were no differences between the fertilization treatments. The amount of the volatile components showed by GC-MS chromatogram was highest in the essential oil obtained by hydrodistillation than SFE. The main volatile constituents of the essential oils were trans-anethole (Hydrodistillation: organic - 52.4%; mineral - 55.0%/ SFE: Hydrodistillation - 62.8%; mineral - 66.8%) and methyl-chavicol (Hydrodistillation: organic - 37.3%; mineral - 38.3%/SFE: organic - 8.4%; mineral - 4.3%). A reduction of methyl-chavicol relative proportion of essential oil obtained by SFE was observed. Cys-anethole, alpha-copaene, trans-cariofilene, germacrene-D, beta-selinene, biciclogermacrene and spathulenol were expressed only in hydrodistillation. The extraction of essential oil by SFE presented larger yield of essential oil than hydrodistillation technique, presenting, however, these essential oils, different phytochemical profiles.Embrapa Meio Ambiente, Lab Prod Nat, Rod SP 340,Km 127,5 Bairro Tanquinho Velho, BR-13820000 Jaguariuna, SP, BrazilUNESP, Fac Cienc Agronom, Dept Horticultura, Botucatu, SP, BrazilCtr Pesquisa Desenvolvimento Recursos Genet, Botucatu, SP, BrazilUniv Estadual Campinas, Fac Engn Alimentos, Dept Engn Alimentos, Campinas, SP, BrazilUNESP, Fac Cienc Agronom, Dept Horticultura, Botucatu, SP, BrazilInt Soc Horticultural ScienceEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Ctr Pesquisa Desenvolvimento Recursos GenetUniversidade Estadual de Campinas (UNICAMP)Aparecida Salgado de Morais, L.Ming, Lin Chau [UNESP]Ortiz Mayo Marques, M.Almeida Meireles, M. A.Mathe, A.Barata, A. M.Carlen, C.Craker, L.2020-12-10T19:31:02Z2020-12-10T19:31:02Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject155-158Xxviii International Horticultural Congress On Science And Horticulture For People (ihc2010): A New Look At Medicinal And Aromatic Plants Seminar. Leuven 1: Int Soc Horticultural Science, v. 925, p. 155-158, 2011.0567-7572http://hdl.handle.net/11449/196029WOS:000314954200022Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengXxviii International Horticultural Congress On Science And Horticulture For People (ihc2010): A New Look At Medicinal And Aromatic Plants Seminarinfo:eu-repo/semantics/openAccess2024-04-30T14:34:34Zoai:repositorio.unesp.br:11449/196029Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:59:09.110712Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Yield and Phytochemical Characterization of Essential Oil from Ocimum selloi B. Obtained by Hydrodistillation and Supercritical Fluid Extraction
title Yield and Phytochemical Characterization of Essential Oil from Ocimum selloi B. Obtained by Hydrodistillation and Supercritical Fluid Extraction
spellingShingle Yield and Phytochemical Characterization of Essential Oil from Ocimum selloi B. Obtained by Hydrodistillation and Supercritical Fluid Extraction
Aparecida Salgado de Morais, L.
methyl-chavicol
trans-anethole
SFE
organic treatment
mineral treatment
title_short Yield and Phytochemical Characterization of Essential Oil from Ocimum selloi B. Obtained by Hydrodistillation and Supercritical Fluid Extraction
title_full Yield and Phytochemical Characterization of Essential Oil from Ocimum selloi B. Obtained by Hydrodistillation and Supercritical Fluid Extraction
title_fullStr Yield and Phytochemical Characterization of Essential Oil from Ocimum selloi B. Obtained by Hydrodistillation and Supercritical Fluid Extraction
title_full_unstemmed Yield and Phytochemical Characterization of Essential Oil from Ocimum selloi B. Obtained by Hydrodistillation and Supercritical Fluid Extraction
title_sort Yield and Phytochemical Characterization of Essential Oil from Ocimum selloi B. Obtained by Hydrodistillation and Supercritical Fluid Extraction
author Aparecida Salgado de Morais, L.
author_facet Aparecida Salgado de Morais, L.
Ming, Lin Chau [UNESP]
Ortiz Mayo Marques, M.
Almeida Meireles, M. A.
Mathe, A.
Barata, A. M.
Carlen, C.
Craker, L.
author_role author
author2 Ming, Lin Chau [UNESP]
Ortiz Mayo Marques, M.
Almeida Meireles, M. A.
Mathe, A.
Barata, A. M.
Carlen, C.
Craker, L.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Estadual Paulista (Unesp)
Ctr Pesquisa Desenvolvimento Recursos Genet
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Aparecida Salgado de Morais, L.
Ming, Lin Chau [UNESP]
Ortiz Mayo Marques, M.
Almeida Meireles, M. A.
Mathe, A.
Barata, A. M.
Carlen, C.
Craker, L.
dc.subject.por.fl_str_mv methyl-chavicol
trans-anethole
SFE
organic treatment
mineral treatment
topic methyl-chavicol
trans-anethole
SFE
organic treatment
mineral treatment
description The yield and chemical composition of essential oils from leaves of Ocimum selloi B. submitted to organic and mineral fertilization, obtained by hydrodistillation and supercritical fluid extraction (SFE) were compared. Essential oil was extracted in a Clevenger-type apparatus for 2 h 30 min and analyzed by GC-MS (Shimadzu, QP 5050-DB-5 capillary column - 30 m x 0.25 mm x 0.25 mu m). Carrier gas was helium (1.7 ml/min); split ratio: 1:30. Temperature program: 50 degrees C, rising to 180 degrees C at 5 degrees C/min, 180 degrees C, rising to 280 degrees C at 10 degrees C/min. Injector temperature: 240 degrees C and detector temperature: 230 degrees C. Identifications of chemical compounds were made by matching their mass spectra and Kovat's indices (IK) values with known compounds reported in the literature. An Applied Separations-apparatus (Speed SFE, model 7071, Allentown, PA, EUA) was used for SFE extractions. They were conducted at pressure 200 bar and temperature 30 degrees C (20 min in static mode and 40 min in dynamic mode). The supercritical CO2 flow rate was (6.8 +/- 0.7) x 10-5 kg-CO2/s. The essential oil collected was immersed in ethylene glycol bath (5 degrees C). The yield of essential oils obtained by SFE was larger than hydrodistillation in both fertilization treatments (279 and 333% for organic and mineral fertilizations, respectively). There were no differences between the fertilization treatments. The amount of the volatile components showed by GC-MS chromatogram was highest in the essential oil obtained by hydrodistillation than SFE. The main volatile constituents of the essential oils were trans-anethole (Hydrodistillation: organic - 52.4%; mineral - 55.0%/ SFE: Hydrodistillation - 62.8%; mineral - 66.8%) and methyl-chavicol (Hydrodistillation: organic - 37.3%; mineral - 38.3%/SFE: organic - 8.4%; mineral - 4.3%). A reduction of methyl-chavicol relative proportion of essential oil obtained by SFE was observed. Cys-anethole, alpha-copaene, trans-cariofilene, germacrene-D, beta-selinene, biciclogermacrene and spathulenol were expressed only in hydrodistillation. The extraction of essential oil by SFE presented larger yield of essential oil than hydrodistillation technique, presenting, however, these essential oils, different phytochemical profiles.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
2020-12-10T19:31:02Z
2020-12-10T19:31:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv Xxviii International Horticultural Congress On Science And Horticulture For People (ihc2010): A New Look At Medicinal And Aromatic Plants Seminar. Leuven 1: Int Soc Horticultural Science, v. 925, p. 155-158, 2011.
0567-7572
http://hdl.handle.net/11449/196029
WOS:000314954200022
identifier_str_mv Xxviii International Horticultural Congress On Science And Horticulture For People (ihc2010): A New Look At Medicinal And Aromatic Plants Seminar. Leuven 1: Int Soc Horticultural Science, v. 925, p. 155-158, 2011.
0567-7572
WOS:000314954200022
url http://hdl.handle.net/11449/196029
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Xxviii International Horticultural Congress On Science And Horticulture For People (ihc2010): A New Look At Medicinal And Aromatic Plants Seminar
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 155-158
dc.publisher.none.fl_str_mv Int Soc Horticultural Science
publisher.none.fl_str_mv Int Soc Horticultural Science
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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