Methyl jasmonate induces selaginellin accumulation in Selaginella convoluta

Detalhes bibliográficos
Autor(a) principal: Reginaldo, Fernanda Priscila Santos
Data de Publicação: 2023
Outros Autores: Bueno, Paula Carolina Pires, Lourenço, Estela Mariana Guimarães, de Matos Costa, Isabelly Cristina, Moreira, Letícia Gondim Lambert, de Araújo Roque, Alan, Barbosa, Euzébio Guimarães, Fett-Neto, Arthur Germano, Cavalheiro, Alberto José [UNESP], Giordani, Raquel Brandt
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s11306-022-01966-9
http://hdl.handle.net/11449/249493
Resumo: Introduction: Selaginellins are specialized metabolites and chemotaxonomic markers for Selaginella species. Despite the growing interest in these compounds as a result of their bioactivities, they are accumulated at low levels in the plant. Hence, their isolation and chemical characterization are often difficult, time consuming, and limiting for biological tests. Elicitation with the phytohormone methyl jasmonate (MeJA) could be a strategy to increase the content of selaginellins addressing their low availability problem, that also impairs pharmacological investigations. Matherials and methods: In this study, we examined MeJA elicitation in Selaginella convoluta plants, a medicinal plant found in northeastern Brazil, by treating them with two different concentrations (MeJA: 50 and 100 µM), followed by chemical profiling after 12, 24 and 48 h after application. Samples were harvested and analyzed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Results and discusscion: MeJA treatment significantly impacted the chemical phenotype. Regarding shoots differences in the time-dependent increased accumulation of all metabolites when plants were subjected to 100 µM MeJA were observed while in roots, most metabolites had their concentrations decreased in a time-dependent fashion at the same conditions. Results support organ, MeJA concentration and time post-treatment dependence of specialized metabolite accumulation, mainly the flavonoids and selaginellins. The amount of Selaginellin G in shoots of MeJA-treated specimens increased in 5.63-fold relative to control. The molecular networking approach allowed for the putative annotation of 64 metabolites, among them, the MeJA treatment followed by targeted metabolome analysis also allowed to annotate seven unprecedented selaginellins. Additionally, the in silico bioactive potential of the annotated selaginellins highlighted targets related to neurodegenerative disorders, antiproliferative, and antiparasitic issues. Taken together, data point out MeJA exposure as a strategy to induce potentially bioactive selaginellins accumulation in S. convoluta, this approach could enable a deep investigation about the metabolic function of these metabolites in the genus as well as regarding pharmacological exploration of the undervalued potential.
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spelling Methyl jasmonate induces selaginellin accumulation in Selaginella convolutaLycophytesMeJAPhenolic derivativesSelaginella convolutaSelaginellaceaeSelaginellinsIntroduction: Selaginellins are specialized metabolites and chemotaxonomic markers for Selaginella species. Despite the growing interest in these compounds as a result of their bioactivities, they are accumulated at low levels in the plant. Hence, their isolation and chemical characterization are often difficult, time consuming, and limiting for biological tests. Elicitation with the phytohormone methyl jasmonate (MeJA) could be a strategy to increase the content of selaginellins addressing their low availability problem, that also impairs pharmacological investigations. Matherials and methods: In this study, we examined MeJA elicitation in Selaginella convoluta plants, a medicinal plant found in northeastern Brazil, by treating them with two different concentrations (MeJA: 50 and 100 µM), followed by chemical profiling after 12, 24 and 48 h after application. Samples were harvested and analyzed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Results and discusscion: MeJA treatment significantly impacted the chemical phenotype. Regarding shoots differences in the time-dependent increased accumulation of all metabolites when plants were subjected to 100 µM MeJA were observed while in roots, most metabolites had their concentrations decreased in a time-dependent fashion at the same conditions. Results support organ, MeJA concentration and time post-treatment dependence of specialized metabolite accumulation, mainly the flavonoids and selaginellins. The amount of Selaginellin G in shoots of MeJA-treated specimens increased in 5.63-fold relative to control. The molecular networking approach allowed for the putative annotation of 64 metabolites, among them, the MeJA treatment followed by targeted metabolome analysis also allowed to annotate seven unprecedented selaginellins. Additionally, the in silico bioactive potential of the annotated selaginellins highlighted targets related to neurodegenerative disorders, antiproliferative, and antiparasitic issues. Taken together, data point out MeJA exposure as a strategy to induce potentially bioactive selaginellins accumulation in S. convoluta, this approach could enable a deep investigation about the metabolic function of these metabolites in the genus as well as regarding pharmacological exploration of the undervalued potential.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Pharmacy Federal University of Rio Grande do Norte (UFRN), RNInstitute of Biology Leiden UniversityInstitute of Chemistry Federal University of Alfenas (UNIFAL), MGMax-Planck Institute of Molecular Plant PhysiologyFaculty of Pharmaceutical Sciences Food and Nutrition Federal University of Mato Grosso Do Sul, MSInstitute for Sustainable Development and Environment, Dunas Park Herbarium, RNLaboratory of Plant Physiology Center for Biotechnology and Department of Botany Federal University of Rio Grande Do Sul (UFRGS), RSInstitute of Chemistry São Paulo State University (UNESP), SPInstitute of Chemistry São Paulo State University (UNESP), SPCNPq: CNPq/INCTBioNat 465637/2014-0Federal University of Rio Grande do Norte (UFRN)Leiden UniversityFederal University of Alfenas (UNIFAL)Max-Planck Institute of Molecular Plant PhysiologyFederal University of Mato Grosso Do SulInstitute for Sustainable Development and EnvironmentFederal University of Rio Grande Do Sul (UFRGS)Universidade Estadual Paulista (UNESP)Reginaldo, Fernanda Priscila SantosBueno, Paula Carolina PiresLourenço, Estela Mariana Guimarãesde Matos Costa, Isabelly CristinaMoreira, Letícia Gondim Lambertde Araújo Roque, AlanBarbosa, Euzébio GuimarãesFett-Neto, Arthur GermanoCavalheiro, Alberto José [UNESP]Giordani, Raquel Brandt2023-07-29T16:01:07Z2023-07-29T16:01:07Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s11306-022-01966-9Metabolomics, v. 19, n. 1, 2023.1573-38901573-3882http://hdl.handle.net/11449/24949310.1007/s11306-022-01966-92-s2.0-85144483805Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMetabolomicsinfo:eu-repo/semantics/openAccess2023-07-29T16:01:07Zoai:repositorio.unesp.br:11449/249493Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T16:01:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Methyl jasmonate induces selaginellin accumulation in Selaginella convoluta
title Methyl jasmonate induces selaginellin accumulation in Selaginella convoluta
spellingShingle Methyl jasmonate induces selaginellin accumulation in Selaginella convoluta
Reginaldo, Fernanda Priscila Santos
Lycophytes
MeJA
Phenolic derivatives
Selaginella convoluta
Selaginellaceae
Selaginellins
title_short Methyl jasmonate induces selaginellin accumulation in Selaginella convoluta
title_full Methyl jasmonate induces selaginellin accumulation in Selaginella convoluta
title_fullStr Methyl jasmonate induces selaginellin accumulation in Selaginella convoluta
title_full_unstemmed Methyl jasmonate induces selaginellin accumulation in Selaginella convoluta
title_sort Methyl jasmonate induces selaginellin accumulation in Selaginella convoluta
author Reginaldo, Fernanda Priscila Santos
author_facet Reginaldo, Fernanda Priscila Santos
Bueno, Paula Carolina Pires
Lourenço, Estela Mariana Guimarães
de Matos Costa, Isabelly Cristina
Moreira, Letícia Gondim Lambert
de Araújo Roque, Alan
Barbosa, Euzébio Guimarães
Fett-Neto, Arthur Germano
Cavalheiro, Alberto José [UNESP]
Giordani, Raquel Brandt
author_role author
author2 Bueno, Paula Carolina Pires
Lourenço, Estela Mariana Guimarães
de Matos Costa, Isabelly Cristina
Moreira, Letícia Gondim Lambert
de Araújo Roque, Alan
Barbosa, Euzébio Guimarães
Fett-Neto, Arthur Germano
Cavalheiro, Alberto José [UNESP]
Giordani, Raquel Brandt
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal University of Rio Grande do Norte (UFRN)
Leiden University
Federal University of Alfenas (UNIFAL)
Max-Planck Institute of Molecular Plant Physiology
Federal University of Mato Grosso Do Sul
Institute for Sustainable Development and Environment
Federal University of Rio Grande Do Sul (UFRGS)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Reginaldo, Fernanda Priscila Santos
Bueno, Paula Carolina Pires
Lourenço, Estela Mariana Guimarães
de Matos Costa, Isabelly Cristina
Moreira, Letícia Gondim Lambert
de Araújo Roque, Alan
Barbosa, Euzébio Guimarães
Fett-Neto, Arthur Germano
Cavalheiro, Alberto José [UNESP]
Giordani, Raquel Brandt
dc.subject.por.fl_str_mv Lycophytes
MeJA
Phenolic derivatives
Selaginella convoluta
Selaginellaceae
Selaginellins
topic Lycophytes
MeJA
Phenolic derivatives
Selaginella convoluta
Selaginellaceae
Selaginellins
description Introduction: Selaginellins are specialized metabolites and chemotaxonomic markers for Selaginella species. Despite the growing interest in these compounds as a result of their bioactivities, they are accumulated at low levels in the plant. Hence, their isolation and chemical characterization are often difficult, time consuming, and limiting for biological tests. Elicitation with the phytohormone methyl jasmonate (MeJA) could be a strategy to increase the content of selaginellins addressing their low availability problem, that also impairs pharmacological investigations. Matherials and methods: In this study, we examined MeJA elicitation in Selaginella convoluta plants, a medicinal plant found in northeastern Brazil, by treating them with two different concentrations (MeJA: 50 and 100 µM), followed by chemical profiling after 12, 24 and 48 h after application. Samples were harvested and analyzed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Results and discusscion: MeJA treatment significantly impacted the chemical phenotype. Regarding shoots differences in the time-dependent increased accumulation of all metabolites when plants were subjected to 100 µM MeJA were observed while in roots, most metabolites had their concentrations decreased in a time-dependent fashion at the same conditions. Results support organ, MeJA concentration and time post-treatment dependence of specialized metabolite accumulation, mainly the flavonoids and selaginellins. The amount of Selaginellin G in shoots of MeJA-treated specimens increased in 5.63-fold relative to control. The molecular networking approach allowed for the putative annotation of 64 metabolites, among them, the MeJA treatment followed by targeted metabolome analysis also allowed to annotate seven unprecedented selaginellins. Additionally, the in silico bioactive potential of the annotated selaginellins highlighted targets related to neurodegenerative disorders, antiproliferative, and antiparasitic issues. Taken together, data point out MeJA exposure as a strategy to induce potentially bioactive selaginellins accumulation in S. convoluta, this approach could enable a deep investigation about the metabolic function of these metabolites in the genus as well as regarding pharmacological exploration of the undervalued potential.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T16:01:07Z
2023-07-29T16:01:07Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s11306-022-01966-9
Metabolomics, v. 19, n. 1, 2023.
1573-3890
1573-3882
http://hdl.handle.net/11449/249493
10.1007/s11306-022-01966-9
2-s2.0-85144483805
url http://dx.doi.org/10.1007/s11306-022-01966-9
http://hdl.handle.net/11449/249493
identifier_str_mv Metabolomics, v. 19, n. 1, 2023.
1573-3890
1573-3882
10.1007/s11306-022-01966-9
2-s2.0-85144483805
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Metabolomics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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