Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae)
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1471-2156-12-65 http://hdl.handle.net/11449/18734 |
Resumo: | Background: The Characidium (a Neotropical fish group) have a conserved diploid number (2n = 50), but show remarkable differences among species and populations in relation to sex chromosome systems and location of nucleolus organizer regions (NOR). In this study, we isolated a W-specific probe for the Characidium and characterized six Characidium species/populations using cytogenetic procedures. We analyzed the origin and differentiation of sex and NOR-bearing chromosomes by chromosome painting in populations of Characidium to reveal their evolution, phylogeny, and biogeography.Results: A W-specific probe for efficient chromosome painting was isolated by microdissection and degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) amplification of W chromosomes from C. gomesi. The W probe generated weak signals dispersed on the proto sex chromosomes in C. zebra, dispersed signals in both W and Z chromosomes in C. lauroi and, in C. gomesi populations revealed a proximal site on the long arms of the Z chromosome and the entire W chromosome. All populations showed small terminal W probe sites in some autosomes. The 18S rDNA revealed distinctive patterns for each analyzed species/population with regard to proto sex chromosome, sex chromosome pair, and autosome location.Conclusions: The results from dual-color fluorescence in situ hybridization (dual-color FISH) using W and 18S rDNA probes allowed us to infer the putative evolutionary pathways for the differentiation of sex chromosomes and NORs, from structural rearrangements in a sex proto-chromosome, followed by gene erosion and heterochromatin amplification, morphological differentiation of the sex chromosomal pair, and NOR transposition, giving rise to the distinctive patterns observed among species/populations of Characidium. Biogeographic isolation and differentiation of sex chromosomes seem to have played a major role in the speciation process in this group of fish. |
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Repositório Institucional da UNESP |
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Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae)Background: The Characidium (a Neotropical fish group) have a conserved diploid number (2n = 50), but show remarkable differences among species and populations in relation to sex chromosome systems and location of nucleolus organizer regions (NOR). In this study, we isolated a W-specific probe for the Characidium and characterized six Characidium species/populations using cytogenetic procedures. We analyzed the origin and differentiation of sex and NOR-bearing chromosomes by chromosome painting in populations of Characidium to reveal their evolution, phylogeny, and biogeography.Results: A W-specific probe for efficient chromosome painting was isolated by microdissection and degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) amplification of W chromosomes from C. gomesi. The W probe generated weak signals dispersed on the proto sex chromosomes in C. zebra, dispersed signals in both W and Z chromosomes in C. lauroi and, in C. gomesi populations revealed a proximal site on the long arms of the Z chromosome and the entire W chromosome. All populations showed small terminal W probe sites in some autosomes. The 18S rDNA revealed distinctive patterns for each analyzed species/population with regard to proto sex chromosome, sex chromosome pair, and autosome location.Conclusions: The results from dual-color fluorescence in situ hybridization (dual-color FISH) using W and 18S rDNA probes allowed us to infer the putative evolutionary pathways for the differentiation of sex chromosomes and NORs, from structural rearrangements in a sex proto-chromosome, followed by gene erosion and heterochromatin amplification, morphological differentiation of the sex chromosomal pair, and NOR transposition, giving rise to the distinctive patterns observed among species/populations of Characidium. Biogeographic isolation and differentiation of sex chromosomes seem to have played a major role in the speciation process in this group of fish.Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná (FAADCT/PR)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, BR-84030900 Ponta Grossa, PR, BrazilUniv Estadual Paulista, Dept Morfol, BR-18618970 Botucatu, SP, BrazilUniv Fed Sao Carlos, Dept Genet & Evolucao, BR-13565905 Sao Carlos, SP, BrazilUniv Estadual Paulista, Dept Morfol, BR-18618970 Botucatu, SP, BrazilBiomed Central Ltd.Univ Estadual Ponta GrossaUniversidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Machado, Tatiana C.Pansonato-Alves, Jose C. [UNESP]Pucci, Marcela B.Nogaroto, VivianeAlmeida, Mara C.Oliveira, Claudio [UNESP]Foresti, Fausto [UNESP]Bertollo, Luiz A. C.Moreira-Filho, OrlandoArtoni, Roberto F.Vicari, Marcelo R.2014-05-20T13:52:26Z2014-05-20T13:52:26Z2011-07-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1186/1471-2156-12-65Bmc Genetics. London: Biomed Central Ltd., v. 12, p. 8, 2011.1471-2156http://hdl.handle.net/11449/1873410.1186/1471-2156-12-65WOS:000294203600001WOS000294203600001.pdf0804793944846367Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Genetics2.4691,160info:eu-repo/semantics/openAccess2023-11-12T06:14:04Zoai:repositorio.unesp.br:11449/18734Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:30:18.274660Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
title |
Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
spellingShingle |
Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) Machado, Tatiana C. |
title_short |
Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
title_full |
Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
title_fullStr |
Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
title_full_unstemmed |
Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
title_sort |
Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
author |
Machado, Tatiana C. |
author_facet |
Machado, Tatiana C. Pansonato-Alves, Jose C. [UNESP] Pucci, Marcela B. Nogaroto, Viviane Almeida, Mara C. Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] Bertollo, Luiz A. C. Moreira-Filho, Orlando Artoni, Roberto F. Vicari, Marcelo R. |
author_role |
author |
author2 |
Pansonato-Alves, Jose C. [UNESP] Pucci, Marcela B. Nogaroto, Viviane Almeida, Mara C. Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] Bertollo, Luiz A. C. Moreira-Filho, Orlando Artoni, Roberto F. Vicari, Marcelo R. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Estadual Ponta Grossa Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Machado, Tatiana C. Pansonato-Alves, Jose C. [UNESP] Pucci, Marcela B. Nogaroto, Viviane Almeida, Mara C. Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] Bertollo, Luiz A. C. Moreira-Filho, Orlando Artoni, Roberto F. Vicari, Marcelo R. |
description |
Background: The Characidium (a Neotropical fish group) have a conserved diploid number (2n = 50), but show remarkable differences among species and populations in relation to sex chromosome systems and location of nucleolus organizer regions (NOR). In this study, we isolated a W-specific probe for the Characidium and characterized six Characidium species/populations using cytogenetic procedures. We analyzed the origin and differentiation of sex and NOR-bearing chromosomes by chromosome painting in populations of Characidium to reveal their evolution, phylogeny, and biogeography.Results: A W-specific probe for efficient chromosome painting was isolated by microdissection and degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) amplification of W chromosomes from C. gomesi. The W probe generated weak signals dispersed on the proto sex chromosomes in C. zebra, dispersed signals in both W and Z chromosomes in C. lauroi and, in C. gomesi populations revealed a proximal site on the long arms of the Z chromosome and the entire W chromosome. All populations showed small terminal W probe sites in some autosomes. The 18S rDNA revealed distinctive patterns for each analyzed species/population with regard to proto sex chromosome, sex chromosome pair, and autosome location.Conclusions: The results from dual-color fluorescence in situ hybridization (dual-color FISH) using W and 18S rDNA probes allowed us to infer the putative evolutionary pathways for the differentiation of sex chromosomes and NORs, from structural rearrangements in a sex proto-chromosome, followed by gene erosion and heterochromatin amplification, morphological differentiation of the sex chromosomal pair, and NOR transposition, giving rise to the distinctive patterns observed among species/populations of Characidium. Biogeographic isolation and differentiation of sex chromosomes seem to have played a major role in the speciation process in this group of fish. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-07-25 2014-05-20T13:52:26Z 2014-05-20T13:52:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1471-2156-12-65 Bmc Genetics. London: Biomed Central Ltd., v. 12, p. 8, 2011. 1471-2156 http://hdl.handle.net/11449/18734 10.1186/1471-2156-12-65 WOS:000294203600001 WOS000294203600001.pdf 0804793944846367 |
url |
http://dx.doi.org/10.1186/1471-2156-12-65 http://hdl.handle.net/11449/18734 |
identifier_str_mv |
Bmc Genetics. London: Biomed Central Ltd., v. 12, p. 8, 2011. 1471-2156 10.1186/1471-2156-12-65 WOS:000294203600001 WOS000294203600001.pdf 0804793944846367 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Genetics 2.469 1,160 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128818770083840 |