Diazepam influences urinary bioindicator of occupational toluene exposure

Detalhes bibliográficos
Autor(a) principal: Silveira, Alberto Thalison
Data de Publicação: 2016
Outros Autores: Albuquerque, Ana Carolina Campos, Lepera, Jose Salvador [UNESP], Martins, Isarita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.etap.2016.10.012
http://hdl.handle.net/11449/162274
Resumo: In the present study, we investigated the influence of diazepam (DZP) on the excretion of TOL by examining their urinary metabolites, hippuric acid (HA) and ortho-cresol (o-C). Male Wistar rats were exposed to TOL (20 ppm) in a nose-only exposure chamber (6 h/day, 5 days/week for 6 weeks) with simultaneous administration of DZP (10 mg/kg/day). Urinary o-C levels were determined by GC MS, while HA, creatinine (CR), DZP and its metabolite, nordiazepam, were analysed by HPLC-DAD. The results of a Mann-Whitney U test showed that DZP influenced the urinary excretion of o-C (p < 0.05). This pioneering study revealed that there was an interaction between DZP and TOL, probably by the inhibition of the CYP isoforms (CYP2B6, CYP2C8, CYP2E1, and CYP1A2) involved in the oxidative metabolism of the solvent. ThiS is relevant information to be considered in the biomonitoring of occupational toluene exposure. (C) 2016 Elsevier B.V. All rights reserved.
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spelling Diazepam influences urinary bioindicator of occupational toluene exposureTolueneDiazepamOccupational exposureo-CresolHippuric acidIn the present study, we investigated the influence of diazepam (DZP) on the excretion of TOL by examining their urinary metabolites, hippuric acid (HA) and ortho-cresol (o-C). Male Wistar rats were exposed to TOL (20 ppm) in a nose-only exposure chamber (6 h/day, 5 days/week for 6 weeks) with simultaneous administration of DZP (10 mg/kg/day). Urinary o-C levels were determined by GC MS, while HA, creatinine (CR), DZP and its metabolite, nordiazepam, were analysed by HPLC-DAD. The results of a Mann-Whitney U test showed that DZP influenced the urinary excretion of o-C (p < 0.05). This pioneering study revealed that there was an interaction between DZP and TOL, probably by the inhibition of the CYP isoforms (CYP2B6, CYP2C8, CYP2E1, and CYP1A2) involved in the oxidative metabolism of the solvent. ThiS is relevant information to be considered in the biomonitoring of occupational toluene exposure. (C) 2016 Elsevier B.V. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Univ Fed Alfenas, Unifal MG, Lab Toxicant & Drug Anal, Gabriel Monteiro da Silva St 700, BR-37130000 Alfenas, MG, BrazilUNESP, Fac Pharmaceut Sci Araraquara FCFAr, Lab Occupat Toxicol, Araraquara, SP, BrazilUNESP, Fac Pharmaceut Sci Araraquara FCFAr, Lab Occupat Toxicol, Araraquara, SP, BrazilFAPEMIG: APQ-01747-13Elsevier B.V.Univ Fed AlfenasUniversidade Estadual Paulista (Unesp)Silveira, Alberto ThalisonAlbuquerque, Ana Carolina CamposLepera, Jose Salvador [UNESP]Martins, Isarita2018-11-26T17:15:26Z2018-11-26T17:15:26Z2016-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article191-196application/pdfhttp://dx.doi.org/10.1016/j.etap.2016.10.012Environmental Toxicology And Pharmacology. Amsterdam: Elsevier Science Bv, v. 48, p. 191-196, 2016.1382-6689http://hdl.handle.net/11449/16227410.1016/j.etap.2016.10.012WOS:000390517200024WOS000390517200024.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Toxicology And Pharmacology0,813info:eu-repo/semantics/openAccess2024-06-24T14:51:24Zoai:repositorio.unesp.br:11449/162274Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:24:43.642712Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Diazepam influences urinary bioindicator of occupational toluene exposure
title Diazepam influences urinary bioindicator of occupational toluene exposure
spellingShingle Diazepam influences urinary bioindicator of occupational toluene exposure
Silveira, Alberto Thalison
Toluene
Diazepam
Occupational exposure
o-Cresol
Hippuric acid
title_short Diazepam influences urinary bioindicator of occupational toluene exposure
title_full Diazepam influences urinary bioindicator of occupational toluene exposure
title_fullStr Diazepam influences urinary bioindicator of occupational toluene exposure
title_full_unstemmed Diazepam influences urinary bioindicator of occupational toluene exposure
title_sort Diazepam influences urinary bioindicator of occupational toluene exposure
author Silveira, Alberto Thalison
author_facet Silveira, Alberto Thalison
Albuquerque, Ana Carolina Campos
Lepera, Jose Salvador [UNESP]
Martins, Isarita
author_role author
author2 Albuquerque, Ana Carolina Campos
Lepera, Jose Salvador [UNESP]
Martins, Isarita
author2_role author
author
author
dc.contributor.none.fl_str_mv Univ Fed Alfenas
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silveira, Alberto Thalison
Albuquerque, Ana Carolina Campos
Lepera, Jose Salvador [UNESP]
Martins, Isarita
dc.subject.por.fl_str_mv Toluene
Diazepam
Occupational exposure
o-Cresol
Hippuric acid
topic Toluene
Diazepam
Occupational exposure
o-Cresol
Hippuric acid
description In the present study, we investigated the influence of diazepam (DZP) on the excretion of TOL by examining their urinary metabolites, hippuric acid (HA) and ortho-cresol (o-C). Male Wistar rats were exposed to TOL (20 ppm) in a nose-only exposure chamber (6 h/day, 5 days/week for 6 weeks) with simultaneous administration of DZP (10 mg/kg/day). Urinary o-C levels were determined by GC MS, while HA, creatinine (CR), DZP and its metabolite, nordiazepam, were analysed by HPLC-DAD. The results of a Mann-Whitney U test showed that DZP influenced the urinary excretion of o-C (p < 0.05). This pioneering study revealed that there was an interaction between DZP and TOL, probably by the inhibition of the CYP isoforms (CYP2B6, CYP2C8, CYP2E1, and CYP1A2) involved in the oxidative metabolism of the solvent. ThiS is relevant information to be considered in the biomonitoring of occupational toluene exposure. (C) 2016 Elsevier B.V. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-01
2018-11-26T17:15:26Z
2018-11-26T17:15:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.etap.2016.10.012
Environmental Toxicology And Pharmacology. Amsterdam: Elsevier Science Bv, v. 48, p. 191-196, 2016.
1382-6689
http://hdl.handle.net/11449/162274
10.1016/j.etap.2016.10.012
WOS:000390517200024
WOS000390517200024.pdf
url http://dx.doi.org/10.1016/j.etap.2016.10.012
http://hdl.handle.net/11449/162274
identifier_str_mv Environmental Toxicology And Pharmacology. Amsterdam: Elsevier Science Bv, v. 48, p. 191-196, 2016.
1382-6689
10.1016/j.etap.2016.10.012
WOS:000390517200024
WOS000390517200024.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Environmental Toxicology And Pharmacology
0,813
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 191-196
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128356369039360

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