Influence of the Paracoccidioides brasiliensis 14-3-3 and gp43 proteins on the induction of apoptosis in A549 epithelial cells

Detalhes bibliográficos
Autor(a) principal: Silva, Julhiany de Fatima da [UNESP]
Data de Publicação: 2015
Outros Autores: Vicentim, Juliana [UNESP], Oliveira, Haroldo Cesar de [UNESP], Marcos, Caroline Maria [UNESP], Assato, Patricia Akemi [UNESP], Andreotti, Patricia Ferrari [UNESP], Monteiro da Silva, Juliana Leal [UNESP], Soares, Christiane Pienna [UNESP], Benard, Gil, Fusco Almeida, Ana Marisa [UNESP], Mendes-Giannini, Maria José Soares [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400476&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/129357
Resumo: The fungal strain Paracoccidioides brasiliensis remains viable inside of epithelial cells and can induce apoptosis in this population. However, until now, the molecules that participate in this process remained unknown. Thus, this study evaluated the contribution of two P. brasiliensis molecules, the 14-3-3 and glycoprotein of 43 kDa proteins, which had been previously described as extracellular matrix adhesins and apoptosis inductors in human pneumocytes. Accordingly, epithelial cells were treated with these molecules for different periods of time and the expression of the apoptosis regulating-proteins Bak, Bax, Bcl-2, p53 and caspases were evaluated by terminal deoxynucleotidyl transferase dUTP nick end labelling, flow cytometry and real-time polymerase chain reaction analysis. Our results demonstrated that treatment with these molecules induces apoptosis signalling in pulmonary epithelial cells, showing the same pattern of programmed cell-death as that observed during infection with P. brasiliensis. Thus, we could conclude that P. brasiliensis uses these molecules as virulence factors that participate not only in the fungal adhesion process to host cells, but also in other important cellular mechanisms such as apoptosis.
id UNSP_34ce6d3ea472ca62495098f474157666
oai_identifier_str oai:repositorio.unesp.br:11449/129357
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Influence of the Paracoccidioides brasiliensis 14-3-3 and gp43 proteins on the induction of apoptosis in A549 epithelial cellsParacoccidioides brasiliensisAdhesinsApoptosisThe fungal strain Paracoccidioides brasiliensis remains viable inside of epithelial cells and can induce apoptosis in this population. However, until now, the molecules that participate in this process remained unknown. Thus, this study evaluated the contribution of two P. brasiliensis molecules, the 14-3-3 and glycoprotein of 43 kDa proteins, which had been previously described as extracellular matrix adhesins and apoptosis inductors in human pneumocytes. Accordingly, epithelial cells were treated with these molecules for different periods of time and the expression of the apoptosis regulating-proteins Bak, Bax, Bcl-2, p53 and caspases were evaluated by terminal deoxynucleotidyl transferase dUTP nick end labelling, flow cytometry and real-time polymerase chain reaction analysis. Our results demonstrated that treatment with these molecules induces apoptosis signalling in pulmonary epithelial cells, showing the same pattern of programmed cell-death as that observed during infection with P. brasiliensis. Thus, we could conclude that P. brasiliensis uses these molecules as virulence factors that participate not only in the fungal adhesion process to host cells, but also in other important cellular mechanisms such as apoptosis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Anal Clin, Araraquara, SP, BrazilUniv Sao Paulo, Lab Alergia &Imunol Clin &Expt, Sao Paulo, SP, BrazilUniv Sao Paulo, Fac Med, Clin Doencas Infecciosas &Parasitarias, Sao Paulo, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Anal Clin, Araraquara, SP, BrazilFundaco Oswaldo CruzUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Silva, Julhiany de Fatima da [UNESP]Vicentim, Juliana [UNESP]Oliveira, Haroldo Cesar de [UNESP]Marcos, Caroline Maria [UNESP]Assato, Patricia Akemi [UNESP]Andreotti, Patricia Ferrari [UNESP]Monteiro da Silva, Juliana Leal [UNESP]Soares, Christiane Pienna [UNESP]Benard, GilFusco Almeida, Ana Marisa [UNESP]Mendes-Giannini, Maria José Soares [UNESP]2015-10-21T20:55:00Z2015-10-21T20:55:00Z2015-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article476-484application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400476&lng=en&nrm=iso&tlng=enMemorias do Instituto Oswaldo Cruz, v. 110, n. 4, p. 476-484, 2015.0074-0276http://hdl.handle.net/11449/12935710.1590/0074-02760150057S0074-02762015000400476WOS:000356610000004S0074-02762015000400476.pdf17680252903736690000-0002-8059-08260000-0003-1740-7360Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemorias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2023-10-11T06:09:25Zoai:repositorio.unesp.br:11449/129357Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-11T06:09:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Influence of the Paracoccidioides brasiliensis 14-3-3 and gp43 proteins on the induction of apoptosis in A549 epithelial cells
title Influence of the Paracoccidioides brasiliensis 14-3-3 and gp43 proteins on the induction of apoptosis in A549 epithelial cells
spellingShingle Influence of the Paracoccidioides brasiliensis 14-3-3 and gp43 proteins on the induction of apoptosis in A549 epithelial cells
Silva, Julhiany de Fatima da [UNESP]
Paracoccidioides brasiliensis
Adhesins
Apoptosis
title_short Influence of the Paracoccidioides brasiliensis 14-3-3 and gp43 proteins on the induction of apoptosis in A549 epithelial cells
title_full Influence of the Paracoccidioides brasiliensis 14-3-3 and gp43 proteins on the induction of apoptosis in A549 epithelial cells
title_fullStr Influence of the Paracoccidioides brasiliensis 14-3-3 and gp43 proteins on the induction of apoptosis in A549 epithelial cells
title_full_unstemmed Influence of the Paracoccidioides brasiliensis 14-3-3 and gp43 proteins on the induction of apoptosis in A549 epithelial cells
title_sort Influence of the Paracoccidioides brasiliensis 14-3-3 and gp43 proteins on the induction of apoptosis in A549 epithelial cells
author Silva, Julhiany de Fatima da [UNESP]
author_facet Silva, Julhiany de Fatima da [UNESP]
Vicentim, Juliana [UNESP]
Oliveira, Haroldo Cesar de [UNESP]
Marcos, Caroline Maria [UNESP]
Assato, Patricia Akemi [UNESP]
Andreotti, Patricia Ferrari [UNESP]
Monteiro da Silva, Juliana Leal [UNESP]
Soares, Christiane Pienna [UNESP]
Benard, Gil
Fusco Almeida, Ana Marisa [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
author_role author
author2 Vicentim, Juliana [UNESP]
Oliveira, Haroldo Cesar de [UNESP]
Marcos, Caroline Maria [UNESP]
Assato, Patricia Akemi [UNESP]
Andreotti, Patricia Ferrari [UNESP]
Monteiro da Silva, Juliana Leal [UNESP]
Soares, Christiane Pienna [UNESP]
Benard, Gil
Fusco Almeida, Ana Marisa [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Silva, Julhiany de Fatima da [UNESP]
Vicentim, Juliana [UNESP]
Oliveira, Haroldo Cesar de [UNESP]
Marcos, Caroline Maria [UNESP]
Assato, Patricia Akemi [UNESP]
Andreotti, Patricia Ferrari [UNESP]
Monteiro da Silva, Juliana Leal [UNESP]
Soares, Christiane Pienna [UNESP]
Benard, Gil
Fusco Almeida, Ana Marisa [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
dc.subject.por.fl_str_mv Paracoccidioides brasiliensis
Adhesins
Apoptosis
topic Paracoccidioides brasiliensis
Adhesins
Apoptosis
description The fungal strain Paracoccidioides brasiliensis remains viable inside of epithelial cells and can induce apoptosis in this population. However, until now, the molecules that participate in this process remained unknown. Thus, this study evaluated the contribution of two P. brasiliensis molecules, the 14-3-3 and glycoprotein of 43 kDa proteins, which had been previously described as extracellular matrix adhesins and apoptosis inductors in human pneumocytes. Accordingly, epithelial cells were treated with these molecules for different periods of time and the expression of the apoptosis regulating-proteins Bak, Bax, Bcl-2, p53 and caspases were evaluated by terminal deoxynucleotidyl transferase dUTP nick end labelling, flow cytometry and real-time polymerase chain reaction analysis. Our results demonstrated that treatment with these molecules induces apoptosis signalling in pulmonary epithelial cells, showing the same pattern of programmed cell-death as that observed during infection with P. brasiliensis. Thus, we could conclude that P. brasiliensis uses these molecules as virulence factors that participate not only in the fungal adhesion process to host cells, but also in other important cellular mechanisms such as apoptosis.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-21T20:55:00Z
2015-10-21T20:55:00Z
2015-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400476&lng=en&nrm=iso&tlng=en
Memorias do Instituto Oswaldo Cruz, v. 110, n. 4, p. 476-484, 2015.
0074-0276
http://hdl.handle.net/11449/129357
10.1590/0074-02760150057
S0074-02762015000400476
WOS:000356610000004
S0074-02762015000400476.pdf
1768025290373669
0000-0002-8059-0826
0000-0003-1740-7360
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400476&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/129357
identifier_str_mv Memorias do Instituto Oswaldo Cruz, v. 110, n. 4, p. 476-484, 2015.
0074-0276
10.1590/0074-02760150057
S0074-02762015000400476
WOS:000356610000004
S0074-02762015000400476.pdf
1768025290373669
0000-0002-8059-0826
0000-0003-1740-7360
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memorias do Instituto Oswaldo Cruz
2.833
1,172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 476-484
application/pdf
dc.publisher.none.fl_str_mv Fundaco Oswaldo Cruz
publisher.none.fl_str_mv Fundaco Oswaldo Cruz
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799964525324140544