Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration

• Autor(a) principal: Mainardes, Rubiana M.
• Data de Publicação: 2009
• Outros Autores: Gremião, Maria Palmira Daflon [UNESP]
• Tipo de documento: Artigo de conferência
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.4067/S0716-97602009000300010; http://hdl.handle.net/11449/7825
• topic: RP-HPLC; validation; AZT; pharmacokinetics; intranasal delivery

The development and validation of a simple and accurate method based on HPLC with ultraviolet detection for the quantification of zidovudine in rat plasma and its application to a pharmacokinetic study following a single intranasal dose zidovudine is described. Zidovudine was extracted from the plasma using, a single-step deproteinization. Chromatographic separation of zidovudine from interfering components wits achieved with a C-18 reverse phase column, a mobile phase Consisting of a mixture of sodium acetate buffer (55 mM) with pH adjusted to 7.0 and acetonitrile (91: 9 v/v) and UV detection set at 265 run. The method was linear from 100 to 10000 ng.mL(-1) (r(2) >= 0.9995), and zidovudine had a mean recovery from plasma of 92.8%. The coefficient of variation of inter-day and intra-day quality control samples was less than 15%. After a single intranasal dose of zidovudine administered to rats, pharmacokinetic parameters (AUC(0-24), C(max), t(max), t(1/2)) were determined. The proposed method was found to be simple, specific. accurate, and precise and could be applied to the quantitative analysis of clinical pharmacokinetic studies of zidovudine in rats.