Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1982-0224-20150141 http://hdl.handle.net/11449/159012 |
Resumo: | Characiformes is the most cytogenetically studied group of freshwater Actinopterygii, but karyotypical data of several taxa remain unknown. This is the case of Nematocharax, regarded as a monotypic genus and characterized by marked sexual dimorphism. Therefore, we provide the first cytogenetic report of allopatric populations of Nematocharax venustus based on distinct methods of chromosomal banding and fluorescence in situ hybridization (FISH) with repetitive DNA probes (18S and 5S rDNA). The karyotype macrostructure was conserved in all specimens and populations, independently on sex, since they shared a diploid number (2n) of 50 chromosomes divided into 8m+26sm+14st+2a. The heterochromatin was mainly distributed at pericentromeric regions and base-specific fluorochrome staining revealed a single pair bearing GC-rich sites, coincident with nucleolar organizer regions (NORs). On the other hand, interpopulation variation in both number and position of repetitive sequences was observed, particularly in relation to 5S rDNA. Apparently, the short life cycles and restricted dispersal of small characins, such as N. venustus, might have favored the divergence of repetitive DNA among populations, indicating that this species might encompass populations with distinct evolutionary histories, which has important implications for conservation measures. |
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Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences18S rDNA5S rDNACoastal drainagesFISHPopulation cytogeneticsCharaciformes is the most cytogenetically studied group of freshwater Actinopterygii, but karyotypical data of several taxa remain unknown. This is the case of Nematocharax, regarded as a monotypic genus and characterized by marked sexual dimorphism. Therefore, we provide the first cytogenetic report of allopatric populations of Nematocharax venustus based on distinct methods of chromosomal banding and fluorescence in situ hybridization (FISH) with repetitive DNA probes (18S and 5S rDNA). The karyotype macrostructure was conserved in all specimens and populations, independently on sex, since they shared a diploid number (2n) of 50 chromosomes divided into 8m+26sm+14st+2a. The heterochromatin was mainly distributed at pericentromeric regions and base-specific fluorochrome staining revealed a single pair bearing GC-rich sites, coincident with nucleolar organizer regions (NORs). On the other hand, interpopulation variation in both number and position of repetitive sequences was observed, particularly in relation to 5S rDNA. Apparently, the short life cycles and restricted dispersal of small characins, such as N. venustus, might have favored the divergence of repetitive DNA among populations, indicating that this species might encompass populations with distinct evolutionary histories, which has important implications for conservation measures.Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB)Univ Estadual Sudoeste Bahia, Dept Ciencias Biol, Av Jose Moreira Sobrinho S-N, BR-45206190 Jequie, BA, BrazilUniv Fed Sao Carlos, Dept Genet & Evolucao, Rodovia Washington Luis,Km 235 S-N, BR-13565905 Sao Paulo, BrazilUniv Estadual Santa Cruz, Dept Ciencias Biol, Rodovia Jorge Amado,Km 16, BR-45662900 Ilheus, BA, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Zool, Av 24-A,1515, BR-13506970 Sao Paulo, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Zool, Av 24-A,1515, BR-13506970 Sao Paulo, BrazilFundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB): PNE0019/2011Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB): RED0009/2013Soc Brasileira IctiologiaUniv Estadual Sudoeste BahiaUniversidade Federal de São Carlos (UFSCar)Univ Estadual Santa CruzUniversidade Estadual Paulista (Unesp)Barreto, Silvia B.Cioffi, Marcelo B.Medrado, Aline S.Silva, Andre T. [UNESP]Affonso, Paulo R. A. M.Diniz, Debora2018-11-26T15:30:46Z2018-11-26T15:30:46Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1590/1982-0224-20150141Neotropical Ichthyology. Sao Paulo: Soc Brasileira Ictiologia, v. 14, n. 2, 9 p., 2016.1679-6225http://hdl.handle.net/11449/15901210.1590/1982-0224-20150141S1679-62252016000200205WOS:000380831900013S1679-62252016000200205.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeotropical Ichthyology0,559info:eu-repo/semantics/openAccess2024-01-16T06:28:53Zoai:repositorio.unesp.br:11449/159012Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-16T06:28:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences |
title |
Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences |
spellingShingle |
Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences Barreto, Silvia B. 18S rDNA 5S rDNA Coastal drainages FISH Population cytogenetics |
title_short |
Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences |
title_full |
Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences |
title_fullStr |
Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences |
title_full_unstemmed |
Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences |
title_sort |
Allopatric chromosomal variation in Nematocharax venustus Weitzman, Menezes & Britski, 1986 (Actinopterygii: Characiformes) based on mapping of repetitive sequences |
author |
Barreto, Silvia B. |
author_facet |
Barreto, Silvia B. Cioffi, Marcelo B. Medrado, Aline S. Silva, Andre T. [UNESP] Affonso, Paulo R. A. M. Diniz, Debora |
author_role |
author |
author2 |
Cioffi, Marcelo B. Medrado, Aline S. Silva, Andre T. [UNESP] Affonso, Paulo R. A. M. Diniz, Debora |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Univ Estadual Sudoeste Bahia Universidade Federal de São Carlos (UFSCar) Univ Estadual Santa Cruz Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Barreto, Silvia B. Cioffi, Marcelo B. Medrado, Aline S. Silva, Andre T. [UNESP] Affonso, Paulo R. A. M. Diniz, Debora |
dc.subject.por.fl_str_mv |
18S rDNA 5S rDNA Coastal drainages FISH Population cytogenetics |
topic |
18S rDNA 5S rDNA Coastal drainages FISH Population cytogenetics |
description |
Characiformes is the most cytogenetically studied group of freshwater Actinopterygii, but karyotypical data of several taxa remain unknown. This is the case of Nematocharax, regarded as a monotypic genus and characterized by marked sexual dimorphism. Therefore, we provide the first cytogenetic report of allopatric populations of Nematocharax venustus based on distinct methods of chromosomal banding and fluorescence in situ hybridization (FISH) with repetitive DNA probes (18S and 5S rDNA). The karyotype macrostructure was conserved in all specimens and populations, independently on sex, since they shared a diploid number (2n) of 50 chromosomes divided into 8m+26sm+14st+2a. The heterochromatin was mainly distributed at pericentromeric regions and base-specific fluorochrome staining revealed a single pair bearing GC-rich sites, coincident with nucleolar organizer regions (NORs). On the other hand, interpopulation variation in both number and position of repetitive sequences was observed, particularly in relation to 5S rDNA. Apparently, the short life cycles and restricted dispersal of small characins, such as N. venustus, might have favored the divergence of repetitive DNA among populations, indicating that this species might encompass populations with distinct evolutionary histories, which has important implications for conservation measures. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-11-26T15:30:46Z 2018-11-26T15:30:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1982-0224-20150141 Neotropical Ichthyology. Sao Paulo: Soc Brasileira Ictiologia, v. 14, n. 2, 9 p., 2016. 1679-6225 http://hdl.handle.net/11449/159012 10.1590/1982-0224-20150141 S1679-62252016000200205 WOS:000380831900013 S1679-62252016000200205.pdf |
url |
http://dx.doi.org/10.1590/1982-0224-20150141 http://hdl.handle.net/11449/159012 |
identifier_str_mv |
Neotropical Ichthyology. Sao Paulo: Soc Brasileira Ictiologia, v. 14, n. 2, 9 p., 2016. 1679-6225 10.1590/1982-0224-20150141 S1679-62252016000200205 WOS:000380831900013 S1679-62252016000200205.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neotropical Ichthyology 0,559 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Ictiologia |
publisher.none.fl_str_mv |
Soc Brasileira Ictiologia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965636578770944 |