Protein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additives
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.livsci.2023.105269 http://hdl.handle.net/11449/250039 |
Resumo: | Replacement of ionophore feed additives by essential oils demonstrates differences in energy metabolism. The mechanism of action of essential oils depends on the ruminal pH, therefore, feedlot diets with high starch inclusions tend to show greater effectiveness of these natural additives. The aim of this study is to map the proteome of the ruminal epithelium of feedlot Nellore cattle (n = 60) with different additives (monensin, blend of essential oils + exogenous α-amylase) and varying levels of starch (25% and 45%) Two-dimensional polyacrylamide gel electrophoresis was used to separate the proteome of ruminal epithelium. The differentially expressed protein spots were characterized by isoelectric point, molecular mass, volume, and intensity of the spots and identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). Diets using an essential oil blend associated with exogenous amylase promoted greater expression of macromolecules from the degradation of carbohydrates through the glycolytic and ketogenesis pathway. Fourteen proteins were identified in upregulation and the presence of proteins involved in glucose oxidation as well as Hydroxymethylglutaryl–CoA lyase (HMGCL), which catalyzes part of the metabolic intermediate metabolism—a key step in ketogenesis. Our results suggest that there was an increase in glycolysis from the oxidation of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH GAPD), which participates in the first step of acetate and butyrate production and the oxidative decarboxylation of the ruminal epithelium of feedlot Nellore cattle. Monensin increased precursors of propionate, such as methylmalonyl-CoA mutase (MUT), which suggests greater synthesis of propionate via propionyl-CoA upon entry into the citric acid cycle as succinyl-CoA, which can increase feed efficiency and reduces feed intake. |
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Protein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additivesEnergy metabolismMass spectrometryMonensinPropionateReplacement of ionophore feed additives by essential oils demonstrates differences in energy metabolism. The mechanism of action of essential oils depends on the ruminal pH, therefore, feedlot diets with high starch inclusions tend to show greater effectiveness of these natural additives. The aim of this study is to map the proteome of the ruminal epithelium of feedlot Nellore cattle (n = 60) with different additives (monensin, blend of essential oils + exogenous α-amylase) and varying levels of starch (25% and 45%) Two-dimensional polyacrylamide gel electrophoresis was used to separate the proteome of ruminal epithelium. The differentially expressed protein spots were characterized by isoelectric point, molecular mass, volume, and intensity of the spots and identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). Diets using an essential oil blend associated with exogenous amylase promoted greater expression of macromolecules from the degradation of carbohydrates through the glycolytic and ketogenesis pathway. Fourteen proteins were identified in upregulation and the presence of proteins involved in glucose oxidation as well as Hydroxymethylglutaryl–CoA lyase (HMGCL), which catalyzes part of the metabolic intermediate metabolism—a key step in ketogenesis. Our results suggest that there was an increase in glycolysis from the oxidation of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH GAPD), which participates in the first step of acetate and butyrate production and the oxidative decarboxylation of the ruminal epithelium of feedlot Nellore cattle. Monensin increased precursors of propionate, such as methylmalonyl-CoA mutase (MUT), which suggests greater synthesis of propionate via propionyl-CoA upon entry into the citric acid cycle as succinyl-CoA, which can increase feed efficiency and reduces feed intake.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, São PauloDSM Nutritional Products SAUniversity of São Paulo (USP) Bauru School of Dentistry, São PauloUniversity of Nebraska (UNL) Department of BiochemistrySão Paulo State University UNESP College of Technology and Agricultural Sciences, São PauloSão Paulo State University (UNESP) Institute of Biosciences, São PauloSão Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, São PauloSão Paulo State University UNESP College of Technology and Agricultural Sciences, São PauloSão Paulo State University (UNESP) Institute of Biosciences, São PauloCAPES: 001CAPES: 88881.3107432018–0.1FAPESP: FAPESP 19/02538–4Universidade Estadual Paulista (UNESP)DSM Nutritional Products SAUniversidade de São Paulo (USP)University of Nebraska (UNL)Rocha, Leone Campos [UNESP]Assunção, Andrey Sávio de Almeida [UNESP]Martins, Renata Aparecida [UNESP]Carvalho, Victor Valério dePerdigão, AlexandreBuzalaf, Marília Afonso RabeloAdamec, JiriBraga, Camila PereiraMillen, Danilo Domingues [UNESP]Vieira, José Cavalcante Souza [UNESP]Padilha, Pedro de Magalhães [UNESP]2023-07-29T16:16:08Z2023-07-29T16:16:08Z2023-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.livsci.2023.105269Livestock Science, v. 274.1871-1413http://hdl.handle.net/11449/25003910.1016/j.livsci.2023.1052692-s2.0-85161309720Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Scienceinfo:eu-repo/semantics/openAccess2024-05-07T13:48:05Zoai:repositorio.unesp.br:11449/250039Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-07T13:48:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Protein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additives |
title |
Protein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additives |
spellingShingle |
Protein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additives Rocha, Leone Campos [UNESP] Energy metabolism Mass spectrometry Monensin Propionate |
title_short |
Protein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additives |
title_full |
Protein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additives |
title_fullStr |
Protein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additives |
title_full_unstemmed |
Protein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additives |
title_sort |
Protein profiles identified by LC-MS/MS demonstrate change in beta oxidation, ketogenesis, and propionate metabolism in rumen epithelium with different additives |
author |
Rocha, Leone Campos [UNESP] |
author_facet |
Rocha, Leone Campos [UNESP] Assunção, Andrey Sávio de Almeida [UNESP] Martins, Renata Aparecida [UNESP] Carvalho, Victor Valério de Perdigão, Alexandre Buzalaf, Marília Afonso Rabelo Adamec, Jiri Braga, Camila Pereira Millen, Danilo Domingues [UNESP] Vieira, José Cavalcante Souza [UNESP] Padilha, Pedro de Magalhães [UNESP] |
author_role |
author |
author2 |
Assunção, Andrey Sávio de Almeida [UNESP] Martins, Renata Aparecida [UNESP] Carvalho, Victor Valério de Perdigão, Alexandre Buzalaf, Marília Afonso Rabelo Adamec, Jiri Braga, Camila Pereira Millen, Danilo Domingues [UNESP] Vieira, José Cavalcante Souza [UNESP] Padilha, Pedro de Magalhães [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) DSM Nutritional Products SA Universidade de São Paulo (USP) University of Nebraska (UNL) |
dc.contributor.author.fl_str_mv |
Rocha, Leone Campos [UNESP] Assunção, Andrey Sávio de Almeida [UNESP] Martins, Renata Aparecida [UNESP] Carvalho, Victor Valério de Perdigão, Alexandre Buzalaf, Marília Afonso Rabelo Adamec, Jiri Braga, Camila Pereira Millen, Danilo Domingues [UNESP] Vieira, José Cavalcante Souza [UNESP] Padilha, Pedro de Magalhães [UNESP] |
dc.subject.por.fl_str_mv |
Energy metabolism Mass spectrometry Monensin Propionate |
topic |
Energy metabolism Mass spectrometry Monensin Propionate |
description |
Replacement of ionophore feed additives by essential oils demonstrates differences in energy metabolism. The mechanism of action of essential oils depends on the ruminal pH, therefore, feedlot diets with high starch inclusions tend to show greater effectiveness of these natural additives. The aim of this study is to map the proteome of the ruminal epithelium of feedlot Nellore cattle (n = 60) with different additives (monensin, blend of essential oils + exogenous α-amylase) and varying levels of starch (25% and 45%) Two-dimensional polyacrylamide gel electrophoresis was used to separate the proteome of ruminal epithelium. The differentially expressed protein spots were characterized by isoelectric point, molecular mass, volume, and intensity of the spots and identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). Diets using an essential oil blend associated with exogenous amylase promoted greater expression of macromolecules from the degradation of carbohydrates through the glycolytic and ketogenesis pathway. Fourteen proteins were identified in upregulation and the presence of proteins involved in glucose oxidation as well as Hydroxymethylglutaryl–CoA lyase (HMGCL), which catalyzes part of the metabolic intermediate metabolism—a key step in ketogenesis. Our results suggest that there was an increase in glycolysis from the oxidation of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH GAPD), which participates in the first step of acetate and butyrate production and the oxidative decarboxylation of the ruminal epithelium of feedlot Nellore cattle. Monensin increased precursors of propionate, such as methylmalonyl-CoA mutase (MUT), which suggests greater synthesis of propionate via propionyl-CoA upon entry into the citric acid cycle as succinyl-CoA, which can increase feed efficiency and reduces feed intake. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:16:08Z 2023-07-29T16:16:08Z 2023-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.livsci.2023.105269 Livestock Science, v. 274. 1871-1413 http://hdl.handle.net/11449/250039 10.1016/j.livsci.2023.105269 2-s2.0-85161309720 |
url |
http://dx.doi.org/10.1016/j.livsci.2023.105269 http://hdl.handle.net/11449/250039 |
identifier_str_mv |
Livestock Science, v. 274. 1871-1413 10.1016/j.livsci.2023.105269 2-s2.0-85161309720 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Livestock Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965511022280704 |