Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours

Detalhes bibliográficos
Autor(a) principal: Zancanela, Daniela Cervelle [UNESP]
Data de Publicação: 2019
Outros Autores: Funari, Cristiano Soleo [UNESP], Herculano, Rondinelli Donizetti [UNESP], Mello, Vinicius Moreira, Rodrigues, Clenilson Martins, Borges, Felipe Azevedo [UNESP], de Barros, Natan Roberto [UNESP], Marcos, Caroline Maria [UNESP], Almeida, Ana Marisa Fusco [UNESP], Guastaldi, Antônio Carlos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.msec.2018.12.042
http://hdl.handle.net/11449/189982
Resumo: Natural Rubber Latex (NRL) is a biocompatible material with demonstrated capacity to induce vascularisation and tissue regeneration. Propolis is a complex resinous product prepared by Apis mellifera with the aim of protecting beehives against infectious microorganisms. It is flora-dependent and its antimicrobial activity can vary according to its geographical origin. This study compares the incorporation of three different types of propolis into an NRL membrane aiming at optimal controlled release of propolis potential antimicrobial compounds towards Candida albicans whilst keeping NRL mechanical characteristics desirable for wound healing bandage purposes. The propolis samples were classified as red, green and poplar propolis according to their chemical composition determined by ultra-high performance liquid chromatography coupled in series with both UV spectrophotometry and high-resolution mass spectrometry. The Minimum Inhibitory Concentrations (MIC) towards C. albicans were determined before their incorporation into NRL membranes. The release of NRL-propolis components in Simulated Body Fluid (SBF) was monitored by UV–Vis spectroscopy. The antimicrobial activity and the effects of the materials released on mouse fibroblasts were assessed. FTIR analyses were carried out in order to verify the formation of new chemical bonds that might prevent the release of propolis components from the NRL membrane. The mechanical characteristics of the NRL membranes remained adequate after the incorporation of the three types of propolis investigated whilst allowing the release of the red, and poplar propolis most active compounds against C. albicans. At 30 and 50% the released materials (eluates) from the NRL membranes incorporated with red and poplar propolis types were not toxic to fibroblast cells. These results suggest that red and poplar propolis can be incorporated into NRL membranes for the preparation of wound healing dressing.
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spelling Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behavioursAntifungalPropolis releaseRed propolisSkin wound healingNatural Rubber Latex (NRL) is a biocompatible material with demonstrated capacity to induce vascularisation and tissue regeneration. Propolis is a complex resinous product prepared by Apis mellifera with the aim of protecting beehives against infectious microorganisms. It is flora-dependent and its antimicrobial activity can vary according to its geographical origin. This study compares the incorporation of three different types of propolis into an NRL membrane aiming at optimal controlled release of propolis potential antimicrobial compounds towards Candida albicans whilst keeping NRL mechanical characteristics desirable for wound healing bandage purposes. The propolis samples were classified as red, green and poplar propolis according to their chemical composition determined by ultra-high performance liquid chromatography coupled in series with both UV spectrophotometry and high-resolution mass spectrometry. The Minimum Inhibitory Concentrations (MIC) towards C. albicans were determined before their incorporation into NRL membranes. The release of NRL-propolis components in Simulated Body Fluid (SBF) was monitored by UV–Vis spectroscopy. The antimicrobial activity and the effects of the materials released on mouse fibroblasts were assessed. FTIR analyses were carried out in order to verify the formation of new chemical bonds that might prevent the release of propolis components from the NRL membrane. The mechanical characteristics of the NRL membranes remained adequate after the incorporation of the three types of propolis investigated whilst allowing the release of the red, and poplar propolis most active compounds against C. albicans. At 30 and 50% the released materials (eluates) from the NRL membranes incorporated with red and poplar propolis types were not toxic to fibroblast cells. These results suggest that red and poplar propolis can be incorporated into NRL membranes for the preparation of wound healing dressing.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (UNESP) Institute of ChemistrySão Paulo State University (UNESP) Faculty of Agricultural SciencesSão Paulo State University (UNESP) School of Pharmaceutical SciencesBrazilian Agricultural Research Corporation (EMBRAPA) Embrapa AgroenergySão Paulo State University (UNESP) Institute of ChemistrySão Paulo State University (UNESP) Faculty of Agricultural SciencesSão Paulo State University (UNESP) School of Pharmaceutical SciencesFAPESP: 2014/17526-8FAPESP: 2015/11759-3FAPESP: 2016/17048-4FAPESP: 2017/19603-8CNPq: 470261/2012-9Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Zancanela, Daniela Cervelle [UNESP]Funari, Cristiano Soleo [UNESP]Herculano, Rondinelli Donizetti [UNESP]Mello, Vinicius MoreiraRodrigues, Clenilson MartinsBorges, Felipe Azevedo [UNESP]de Barros, Natan Roberto [UNESP]Marcos, Caroline Maria [UNESP]Almeida, Ana Marisa Fusco [UNESP]Guastaldi, Antônio Carlos [UNESP]2019-10-06T16:58:30Z2019-10-06T16:58:30Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article576-582http://dx.doi.org/10.1016/j.msec.2018.12.042Materials Science and Engineering C, v. 97, p. 576-582.1873-01910928-4931http://hdl.handle.net/11449/18998210.1016/j.msec.2018.12.0422-s2.0-85059080534Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science and Engineering Cinfo:eu-repo/semantics/openAccess2024-06-13T17:38:20Zoai:repositorio.unesp.br:11449/189982Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:34:14.703760Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours
title Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours
spellingShingle Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours
Zancanela, Daniela Cervelle [UNESP]
Antifungal
Propolis release
Red propolis
Skin wound healing
title_short Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours
title_full Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours
title_fullStr Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours
title_full_unstemmed Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours
title_sort Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours
author Zancanela, Daniela Cervelle [UNESP]
author_facet Zancanela, Daniela Cervelle [UNESP]
Funari, Cristiano Soleo [UNESP]
Herculano, Rondinelli Donizetti [UNESP]
Mello, Vinicius Moreira
Rodrigues, Clenilson Martins
Borges, Felipe Azevedo [UNESP]
de Barros, Natan Roberto [UNESP]
Marcos, Caroline Maria [UNESP]
Almeida, Ana Marisa Fusco [UNESP]
Guastaldi, Antônio Carlos [UNESP]
author_role author
author2 Funari, Cristiano Soleo [UNESP]
Herculano, Rondinelli Donizetti [UNESP]
Mello, Vinicius Moreira
Rodrigues, Clenilson Martins
Borges, Felipe Azevedo [UNESP]
de Barros, Natan Roberto [UNESP]
Marcos, Caroline Maria [UNESP]
Almeida, Ana Marisa Fusco [UNESP]
Guastaldi, Antônio Carlos [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Zancanela, Daniela Cervelle [UNESP]
Funari, Cristiano Soleo [UNESP]
Herculano, Rondinelli Donizetti [UNESP]
Mello, Vinicius Moreira
Rodrigues, Clenilson Martins
Borges, Felipe Azevedo [UNESP]
de Barros, Natan Roberto [UNESP]
Marcos, Caroline Maria [UNESP]
Almeida, Ana Marisa Fusco [UNESP]
Guastaldi, Antônio Carlos [UNESP]
dc.subject.por.fl_str_mv Antifungal
Propolis release
Red propolis
Skin wound healing
topic Antifungal
Propolis release
Red propolis
Skin wound healing
description Natural Rubber Latex (NRL) is a biocompatible material with demonstrated capacity to induce vascularisation and tissue regeneration. Propolis is a complex resinous product prepared by Apis mellifera with the aim of protecting beehives against infectious microorganisms. It is flora-dependent and its antimicrobial activity can vary according to its geographical origin. This study compares the incorporation of three different types of propolis into an NRL membrane aiming at optimal controlled release of propolis potential antimicrobial compounds towards Candida albicans whilst keeping NRL mechanical characteristics desirable for wound healing bandage purposes. The propolis samples were classified as red, green and poplar propolis according to their chemical composition determined by ultra-high performance liquid chromatography coupled in series with both UV spectrophotometry and high-resolution mass spectrometry. The Minimum Inhibitory Concentrations (MIC) towards C. albicans were determined before their incorporation into NRL membranes. The release of NRL-propolis components in Simulated Body Fluid (SBF) was monitored by UV–Vis spectroscopy. The antimicrobial activity and the effects of the materials released on mouse fibroblasts were assessed. FTIR analyses were carried out in order to verify the formation of new chemical bonds that might prevent the release of propolis components from the NRL membrane. The mechanical characteristics of the NRL membranes remained adequate after the incorporation of the three types of propolis investigated whilst allowing the release of the red, and poplar propolis most active compounds against C. albicans. At 30 and 50% the released materials (eluates) from the NRL membranes incorporated with red and poplar propolis types were not toxic to fibroblast cells. These results suggest that red and poplar propolis can be incorporated into NRL membranes for the preparation of wound healing dressing.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:58:30Z
2019-10-06T16:58:30Z
2019-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.msec.2018.12.042
Materials Science and Engineering C, v. 97, p. 576-582.
1873-0191
0928-4931
http://hdl.handle.net/11449/189982
10.1016/j.msec.2018.12.042
2-s2.0-85059080534
url http://dx.doi.org/10.1016/j.msec.2018.12.042
http://hdl.handle.net/11449/189982
identifier_str_mv Materials Science and Engineering C, v. 97, p. 576-582.
1873-0191
0928-4931
10.1016/j.msec.2018.12.042
2-s2.0-85059080534
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Materials Science and Engineering C
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 576-582
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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