Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.msec.2018.12.042 http://hdl.handle.net/11449/189982 |
Resumo: | Natural Rubber Latex (NRL) is a biocompatible material with demonstrated capacity to induce vascularisation and tissue regeneration. Propolis is a complex resinous product prepared by Apis mellifera with the aim of protecting beehives against infectious microorganisms. It is flora-dependent and its antimicrobial activity can vary according to its geographical origin. This study compares the incorporation of three different types of propolis into an NRL membrane aiming at optimal controlled release of propolis potential antimicrobial compounds towards Candida albicans whilst keeping NRL mechanical characteristics desirable for wound healing bandage purposes. The propolis samples were classified as red, green and poplar propolis according to their chemical composition determined by ultra-high performance liquid chromatography coupled in series with both UV spectrophotometry and high-resolution mass spectrometry. The Minimum Inhibitory Concentrations (MIC) towards C. albicans were determined before their incorporation into NRL membranes. The release of NRL-propolis components in Simulated Body Fluid (SBF) was monitored by UV–Vis spectroscopy. The antimicrobial activity and the effects of the materials released on mouse fibroblasts were assessed. FTIR analyses were carried out in order to verify the formation of new chemical bonds that might prevent the release of propolis components from the NRL membrane. The mechanical characteristics of the NRL membranes remained adequate after the incorporation of the three types of propolis investigated whilst allowing the release of the red, and poplar propolis most active compounds against C. albicans. At 30 and 50% the released materials (eluates) from the NRL membranes incorporated with red and poplar propolis types were not toxic to fibroblast cells. These results suggest that red and poplar propolis can be incorporated into NRL membranes for the preparation of wound healing dressing. |
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Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behavioursAntifungalPropolis releaseRed propolisSkin wound healingNatural Rubber Latex (NRL) is a biocompatible material with demonstrated capacity to induce vascularisation and tissue regeneration. Propolis is a complex resinous product prepared by Apis mellifera with the aim of protecting beehives against infectious microorganisms. It is flora-dependent and its antimicrobial activity can vary according to its geographical origin. This study compares the incorporation of three different types of propolis into an NRL membrane aiming at optimal controlled release of propolis potential antimicrobial compounds towards Candida albicans whilst keeping NRL mechanical characteristics desirable for wound healing bandage purposes. The propolis samples were classified as red, green and poplar propolis according to their chemical composition determined by ultra-high performance liquid chromatography coupled in series with both UV spectrophotometry and high-resolution mass spectrometry. The Minimum Inhibitory Concentrations (MIC) towards C. albicans were determined before their incorporation into NRL membranes. The release of NRL-propolis components in Simulated Body Fluid (SBF) was monitored by UV–Vis spectroscopy. The antimicrobial activity and the effects of the materials released on mouse fibroblasts were assessed. FTIR analyses were carried out in order to verify the formation of new chemical bonds that might prevent the release of propolis components from the NRL membrane. The mechanical characteristics of the NRL membranes remained adequate after the incorporation of the three types of propolis investigated whilst allowing the release of the red, and poplar propolis most active compounds against C. albicans. At 30 and 50% the released materials (eluates) from the NRL membranes incorporated with red and poplar propolis types were not toxic to fibroblast cells. These results suggest that red and poplar propolis can be incorporated into NRL membranes for the preparation of wound healing dressing.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (UNESP) Institute of ChemistrySão Paulo State University (UNESP) Faculty of Agricultural SciencesSão Paulo State University (UNESP) School of Pharmaceutical SciencesBrazilian Agricultural Research Corporation (EMBRAPA) Embrapa AgroenergySão Paulo State University (UNESP) Institute of ChemistrySão Paulo State University (UNESP) Faculty of Agricultural SciencesSão Paulo State University (UNESP) School of Pharmaceutical SciencesFAPESP: 2014/17526-8FAPESP: 2015/11759-3FAPESP: 2016/17048-4FAPESP: 2017/19603-8CNPq: 470261/2012-9Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Zancanela, Daniela Cervelle [UNESP]Funari, Cristiano Soleo [UNESP]Herculano, Rondinelli Donizetti [UNESP]Mello, Vinicius MoreiraRodrigues, Clenilson MartinsBorges, Felipe Azevedo [UNESP]de Barros, Natan Roberto [UNESP]Marcos, Caroline Maria [UNESP]Almeida, Ana Marisa Fusco [UNESP]Guastaldi, Antônio Carlos [UNESP]2019-10-06T16:58:30Z2019-10-06T16:58:30Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article576-582http://dx.doi.org/10.1016/j.msec.2018.12.042Materials Science and Engineering C, v. 97, p. 576-582.1873-01910928-4931http://hdl.handle.net/11449/18998210.1016/j.msec.2018.12.0422-s2.0-85059080534Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science and Engineering Cinfo:eu-repo/semantics/openAccess2024-06-13T17:38:20Zoai:repositorio.unesp.br:11449/189982Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:34:14.703760Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours |
title |
Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours |
spellingShingle |
Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours Zancanela, Daniela Cervelle [UNESP] Antifungal Propolis release Red propolis Skin wound healing |
title_short |
Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours |
title_full |
Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours |
title_fullStr |
Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours |
title_full_unstemmed |
Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours |
title_sort |
Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours |
author |
Zancanela, Daniela Cervelle [UNESP] |
author_facet |
Zancanela, Daniela Cervelle [UNESP] Funari, Cristiano Soleo [UNESP] Herculano, Rondinelli Donizetti [UNESP] Mello, Vinicius Moreira Rodrigues, Clenilson Martins Borges, Felipe Azevedo [UNESP] de Barros, Natan Roberto [UNESP] Marcos, Caroline Maria [UNESP] Almeida, Ana Marisa Fusco [UNESP] Guastaldi, Antônio Carlos [UNESP] |
author_role |
author |
author2 |
Funari, Cristiano Soleo [UNESP] Herculano, Rondinelli Donizetti [UNESP] Mello, Vinicius Moreira Rodrigues, Clenilson Martins Borges, Felipe Azevedo [UNESP] de Barros, Natan Roberto [UNESP] Marcos, Caroline Maria [UNESP] Almeida, Ana Marisa Fusco [UNESP] Guastaldi, Antônio Carlos [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Zancanela, Daniela Cervelle [UNESP] Funari, Cristiano Soleo [UNESP] Herculano, Rondinelli Donizetti [UNESP] Mello, Vinicius Moreira Rodrigues, Clenilson Martins Borges, Felipe Azevedo [UNESP] de Barros, Natan Roberto [UNESP] Marcos, Caroline Maria [UNESP] Almeida, Ana Marisa Fusco [UNESP] Guastaldi, Antônio Carlos [UNESP] |
dc.subject.por.fl_str_mv |
Antifungal Propolis release Red propolis Skin wound healing |
topic |
Antifungal Propolis release Red propolis Skin wound healing |
description |
Natural Rubber Latex (NRL) is a biocompatible material with demonstrated capacity to induce vascularisation and tissue regeneration. Propolis is a complex resinous product prepared by Apis mellifera with the aim of protecting beehives against infectious microorganisms. It is flora-dependent and its antimicrobial activity can vary according to its geographical origin. This study compares the incorporation of three different types of propolis into an NRL membrane aiming at optimal controlled release of propolis potential antimicrobial compounds towards Candida albicans whilst keeping NRL mechanical characteristics desirable for wound healing bandage purposes. The propolis samples were classified as red, green and poplar propolis according to their chemical composition determined by ultra-high performance liquid chromatography coupled in series with both UV spectrophotometry and high-resolution mass spectrometry. The Minimum Inhibitory Concentrations (MIC) towards C. albicans were determined before their incorporation into NRL membranes. The release of NRL-propolis components in Simulated Body Fluid (SBF) was monitored by UV–Vis spectroscopy. The antimicrobial activity and the effects of the materials released on mouse fibroblasts were assessed. FTIR analyses were carried out in order to verify the formation of new chemical bonds that might prevent the release of propolis components from the NRL membrane. The mechanical characteristics of the NRL membranes remained adequate after the incorporation of the three types of propolis investigated whilst allowing the release of the red, and poplar propolis most active compounds against C. albicans. At 30 and 50% the released materials (eluates) from the NRL membranes incorporated with red and poplar propolis types were not toxic to fibroblast cells. These results suggest that red and poplar propolis can be incorporated into NRL membranes for the preparation of wound healing dressing. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:58:30Z 2019-10-06T16:58:30Z 2019-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.msec.2018.12.042 Materials Science and Engineering C, v. 97, p. 576-582. 1873-0191 0928-4931 http://hdl.handle.net/11449/189982 10.1016/j.msec.2018.12.042 2-s2.0-85059080534 |
url |
http://dx.doi.org/10.1016/j.msec.2018.12.042 http://hdl.handle.net/11449/189982 |
identifier_str_mv |
Materials Science and Engineering C, v. 97, p. 576-582. 1873-0191 0928-4931 10.1016/j.msec.2018.12.042 2-s2.0-85059080534 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Materials Science and Engineering C |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
576-582 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128828012232704 |