Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/mbo3.706 http://hdl.handle.net/11449/171396 |
Resumo: | Xanthomonas citri subsp. citri (Xac) is the causative agent of citrus canker, a plant disease that significantly impacts citriculture. In earlier work, we showed that alkylated derivatives of gallic acid have antibacterial action against Xac and target both the cell division protein FtsZ and membrane integrity in Bacillus subtilis. Here, we have purified native XacFtsZ and characterized its GTP hydrolysis and polymerization properties. In a surprising manner, inhibition of XacFtsZ activity by alkyl gallates is not as strong as observed earlier with B. subtilis FtsZ. As the alkyl gallates efficiently permeabilize Xac membranes, we propose that this is the primary mode of antibacterial action of these compounds. |
id |
UNSP_35e4bc1119fb1495d078bdc8a964cd1e |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/171396 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citriantibacterialscell divisionFtsZgallatesgallic acidGTPasemembrane permeabilizationXanthomonas citri subsp. citri (Xac) is the causative agent of citrus canker, a plant disease that significantly impacts citriculture. In earlier work, we showed that alkylated derivatives of gallic acid have antibacterial action against Xac and target both the cell division protein FtsZ and membrane integrity in Bacillus subtilis. Here, we have purified native XacFtsZ and characterized its GTP hydrolysis and polymerization properties. In a surprising manner, inhibition of XacFtsZ activity by alkyl gallates is not as strong as observed earlier with B. subtilis FtsZ. As the alkyl gallates efficiently permeabilize Xac membranes, we propose that this is the primary mode of antibacterial action of these compounds.Department of Molecular Microbiology Groningen Biomolecular Sciences and Biotechnology Institute University of GroningenLaboratory of Antibiotics and Chemotherapeutics Department of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP)Department of Chemical Engineering Biotechnology and Environmental Technology University of Southern DenmarkLaboratory of Antibiotics and Chemotherapeutics Department of Chemistry and Environmental Sciences Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP)University of GroningenUniversidade Estadual Paulista (Unesp)University of Southern DenmarkKopacz, Malgorzata M.Lorenzoni, André S. G.Polaquini, Carlos R. [UNESP]Regasini, Luis O. [UNESP]Scheffers, Dirk-Jan2018-12-11T16:55:10Z2018-12-11T16:55:10Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1002/mbo3.706MicrobiologyOpen.2045-8827http://hdl.handle.net/11449/17139610.1002/mbo3.7062-s2.0-850526623702-s2.0-85052662370.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobiologyOpen1,158info:eu-repo/semantics/openAccess2023-10-02T06:04:55Zoai:repositorio.unesp.br:11449/171396Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:46:12.866800Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri |
title |
Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri |
spellingShingle |
Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri Kopacz, Malgorzata M. antibacterials cell division FtsZ gallates gallic acid GTPase membrane permeabilization |
title_short |
Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri |
title_full |
Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri |
title_fullStr |
Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri |
title_full_unstemmed |
Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri |
title_sort |
Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri |
author |
Kopacz, Malgorzata M. |
author_facet |
Kopacz, Malgorzata M. Lorenzoni, André S. G. Polaquini, Carlos R. [UNESP] Regasini, Luis O. [UNESP] Scheffers, Dirk-Jan |
author_role |
author |
author2 |
Lorenzoni, André S. G. Polaquini, Carlos R. [UNESP] Regasini, Luis O. [UNESP] Scheffers, Dirk-Jan |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
University of Groningen Universidade Estadual Paulista (Unesp) University of Southern Denmark |
dc.contributor.author.fl_str_mv |
Kopacz, Malgorzata M. Lorenzoni, André S. G. Polaquini, Carlos R. [UNESP] Regasini, Luis O. [UNESP] Scheffers, Dirk-Jan |
dc.subject.por.fl_str_mv |
antibacterials cell division FtsZ gallates gallic acid GTPase membrane permeabilization |
topic |
antibacterials cell division FtsZ gallates gallic acid GTPase membrane permeabilization |
description |
Xanthomonas citri subsp. citri (Xac) is the causative agent of citrus canker, a plant disease that significantly impacts citriculture. In earlier work, we showed that alkylated derivatives of gallic acid have antibacterial action against Xac and target both the cell division protein FtsZ and membrane integrity in Bacillus subtilis. Here, we have purified native XacFtsZ and characterized its GTP hydrolysis and polymerization properties. In a surprising manner, inhibition of XacFtsZ activity by alkyl gallates is not as strong as observed earlier with B. subtilis FtsZ. As the alkyl gallates efficiently permeabilize Xac membranes, we propose that this is the primary mode of antibacterial action of these compounds. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:55:10Z 2018-12-11T16:55:10Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/mbo3.706 MicrobiologyOpen. 2045-8827 http://hdl.handle.net/11449/171396 10.1002/mbo3.706 2-s2.0-85052662370 2-s2.0-85052662370.pdf |
url |
http://dx.doi.org/10.1002/mbo3.706 http://hdl.handle.net/11449/171396 |
identifier_str_mv |
MicrobiologyOpen. 2045-8827 10.1002/mbo3.706 2-s2.0-85052662370 2-s2.0-85052662370.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
MicrobiologyOpen 1,158 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128274281267200 |