Ceftriaxone pharmacokinetics by new simple and sensitive ultra-high-performance liquid chromatography method

Detalhes bibliográficos
Autor(a) principal: Campos, Michel Leandro [UNESP]
Data de Publicação: 2017
Outros Autores: de Moura Alonso, Juliana [UNESP], dos Santos Martins, Evelin [UNESP], Oliveira, Jonata Augusto [UNESP], Hussni, Carlos Alberto [UNESP], Peccinini, Rosângela Gonçalves [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.diagmicrobio.2017.02.012
http://hdl.handle.net/11449/174315
Resumo: Ceftriaxone is a cephalosporin antibiotic with a potent antimicrobial activity and excellent penetration in most body fluids such as pleural, peritoneal, spinal and brain. These facts contribute to the application of ceftriaxone in the treatment of bacterial peritonitis, an abdominal disorder in veterinary medicine, with potential risk of death. The determination of ceftriaxone levels in plasma and peritoneal fluid may be used to assess the pharmacokinetic profile at various instances of administration and allows observing if the concentrations needed are being achieved. Therefore a method was developed and validated for the determination of ceftriaxone in plasma and peritoneal fluid which after was applied in a pharmacokinetic profile study. The bioanalytical method validation was performed according to widely acceptable experiments. Two horses were used as a model of the method applicability; ceftriaxone was intraperitoneally administered to these animals as a single dose. The plasma and peritoneal fluid analysis were performed using an UHPLC system in reverse phase chromatography mode in fully validated conditions. The methods have shown linearity between 0.49 and 500 μg/mL for plasma, and between 0.24 and 500 μg/mL for peritoneal fluid. The quantitative analysis of ceftriaxone in these matrices allows monitoring of the therapy. This method showed improved sensitivity as well as the quantitation in peritoneal fluid.
id UNSP_36409f06ced38c3b5dcf1b4e83b6640e
oai_identifier_str oai:repositorio.unesp.br:11449/174315
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Ceftriaxone pharmacokinetics by new simple and sensitive ultra-high-performance liquid chromatography methodBioanalytical method validationCeftriaxonePharmacokineticsUHPLCCeftriaxone is a cephalosporin antibiotic with a potent antimicrobial activity and excellent penetration in most body fluids such as pleural, peritoneal, spinal and brain. These facts contribute to the application of ceftriaxone in the treatment of bacterial peritonitis, an abdominal disorder in veterinary medicine, with potential risk of death. The determination of ceftriaxone levels in plasma and peritoneal fluid may be used to assess the pharmacokinetic profile at various instances of administration and allows observing if the concentrations needed are being achieved. Therefore a method was developed and validated for the determination of ceftriaxone in plasma and peritoneal fluid which after was applied in a pharmacokinetic profile study. The bioanalytical method validation was performed according to widely acceptable experiments. Two horses were used as a model of the method applicability; ceftriaxone was intraperitoneally administered to these animals as a single dose. The plasma and peritoneal fluid analysis were performed using an UHPLC system in reverse phase chromatography mode in fully validated conditions. The methods have shown linearity between 0.49 and 500 μg/mL for plasma, and between 0.24 and 500 μg/mL for peritoneal fluid. The quantitative analysis of ceftriaxone in these matrices allows monitoring of the therapy. This method showed improved sensitivity as well as the quantitation in peritoneal fluid.Department of Natural Active Principles and Toxicology School of Pharmaceutical Sciences UNESP–Univ Estadual PaulistaDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science UNESP–Univ Estadual PaulistaDepartment of Natural Active Principles and Toxicology School of Pharmaceutical Sciences UNESP–Univ Estadual PaulistaDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science UNESP–Univ Estadual PaulistaUniversidade Estadual Paulista (Unesp)Campos, Michel Leandro [UNESP]de Moura Alonso, Juliana [UNESP]dos Santos Martins, Evelin [UNESP]Oliveira, Jonata Augusto [UNESP]Hussni, Carlos Alberto [UNESP]Peccinini, Rosângela Gonçalves [UNESP]2018-12-11T17:10:29Z2018-12-11T17:10:29Z2017-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article95-99application/pdfhttp://dx.doi.org/10.1016/j.diagmicrobio.2017.02.012Diagnostic Microbiology and Infectious Disease, v. 88, n. 1, p. 95-99, 2017.1879-00700732-8893http://hdl.handle.net/11449/17431510.1016/j.diagmicrobio.2017.02.0122-s2.0-850147861412-s2.0-85014786141.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDiagnostic Microbiology and Infectious Disease1,157info:eu-repo/semantics/openAccess2024-06-24T14:51:41Zoai:repositorio.unesp.br:11449/174315Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:06:20.740600Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ceftriaxone pharmacokinetics by new simple and sensitive ultra-high-performance liquid chromatography method
title Ceftriaxone pharmacokinetics by new simple and sensitive ultra-high-performance liquid chromatography method
spellingShingle Ceftriaxone pharmacokinetics by new simple and sensitive ultra-high-performance liquid chromatography method
Campos, Michel Leandro [UNESP]
Bioanalytical method validation
Ceftriaxone
Pharmacokinetics
UHPLC
title_short Ceftriaxone pharmacokinetics by new simple and sensitive ultra-high-performance liquid chromatography method
title_full Ceftriaxone pharmacokinetics by new simple and sensitive ultra-high-performance liquid chromatography method
title_fullStr Ceftriaxone pharmacokinetics by new simple and sensitive ultra-high-performance liquid chromatography method
title_full_unstemmed Ceftriaxone pharmacokinetics by new simple and sensitive ultra-high-performance liquid chromatography method
title_sort Ceftriaxone pharmacokinetics by new simple and sensitive ultra-high-performance liquid chromatography method
author Campos, Michel Leandro [UNESP]
author_facet Campos, Michel Leandro [UNESP]
de Moura Alonso, Juliana [UNESP]
dos Santos Martins, Evelin [UNESP]
Oliveira, Jonata Augusto [UNESP]
Hussni, Carlos Alberto [UNESP]
Peccinini, Rosângela Gonçalves [UNESP]
author_role author
author2 de Moura Alonso, Juliana [UNESP]
dos Santos Martins, Evelin [UNESP]
Oliveira, Jonata Augusto [UNESP]
Hussni, Carlos Alberto [UNESP]
Peccinini, Rosângela Gonçalves [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Campos, Michel Leandro [UNESP]
de Moura Alonso, Juliana [UNESP]
dos Santos Martins, Evelin [UNESP]
Oliveira, Jonata Augusto [UNESP]
Hussni, Carlos Alberto [UNESP]
Peccinini, Rosângela Gonçalves [UNESP]
dc.subject.por.fl_str_mv Bioanalytical method validation
Ceftriaxone
Pharmacokinetics
UHPLC
topic Bioanalytical method validation
Ceftriaxone
Pharmacokinetics
UHPLC
description Ceftriaxone is a cephalosporin antibiotic with a potent antimicrobial activity and excellent penetration in most body fluids such as pleural, peritoneal, spinal and brain. These facts contribute to the application of ceftriaxone in the treatment of bacterial peritonitis, an abdominal disorder in veterinary medicine, with potential risk of death. The determination of ceftriaxone levels in plasma and peritoneal fluid may be used to assess the pharmacokinetic profile at various instances of administration and allows observing if the concentrations needed are being achieved. Therefore a method was developed and validated for the determination of ceftriaxone in plasma and peritoneal fluid which after was applied in a pharmacokinetic profile study. The bioanalytical method validation was performed according to widely acceptable experiments. Two horses were used as a model of the method applicability; ceftriaxone was intraperitoneally administered to these animals as a single dose. The plasma and peritoneal fluid analysis were performed using an UHPLC system in reverse phase chromatography mode in fully validated conditions. The methods have shown linearity between 0.49 and 500 μg/mL for plasma, and between 0.24 and 500 μg/mL for peritoneal fluid. The quantitative analysis of ceftriaxone in these matrices allows monitoring of the therapy. This method showed improved sensitivity as well as the quantitation in peritoneal fluid.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-01
2018-12-11T17:10:29Z
2018-12-11T17:10:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.diagmicrobio.2017.02.012
Diagnostic Microbiology and Infectious Disease, v. 88, n. 1, p. 95-99, 2017.
1879-0070
0732-8893
http://hdl.handle.net/11449/174315
10.1016/j.diagmicrobio.2017.02.012
2-s2.0-85014786141
2-s2.0-85014786141.pdf
url http://dx.doi.org/10.1016/j.diagmicrobio.2017.02.012
http://hdl.handle.net/11449/174315
identifier_str_mv Diagnostic Microbiology and Infectious Disease, v. 88, n. 1, p. 95-99, 2017.
1879-0070
0732-8893
10.1016/j.diagmicrobio.2017.02.012
2-s2.0-85014786141
2-s2.0-85014786141.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Diagnostic Microbiology and Infectious Disease
1,157
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 95-99
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128895016239104