Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular study
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2020.104826 http://hdl.handle.net/11449/200767 |
Resumo: | Objective: Identification of specific bacteria in root canals (RCs) in distinct clinical conditions can support the comprehension of pathological processes. Thus, the objective of this clinical study was to investigate the presence of F. alocis in RCs of teeth with primary endodontic infection (PEI) and with persistent/secondary endodontic infection (SEI) by using molecular techniques. It was also aimed to associate its presence with the clinical features. In addition, the levels of F. alocis as well as the total bacterial cells in the samples were also quantitated. Design: One hundred teeth (50 PEI and 50 SEI) were included. Microbial samples were performed using sterile paper points and assessed by using nested PCR and quantitative Real Time PCR (qPCR). The prevalence of F.alocis in RCs from PEI and SEI were compared by chi-square analysis. Fisher´s exact test or Pearson Chi-square, when appropriate, was used to test associations between clinical and radiographic features and the presence of F. alocis. Significance level was set at 5%. Results: F. alocis was detected in 23 and 28 (PEI) and 12 and 11 (SEI) RCs using Nested PCR and qPCR, respectively. Statistically significant associations were found between the presence of F. alocis and PEI, pain, wet canals, swelling, abscess and purulent exudate (P < 0.05). Total bacterial count was similar in both conditions (P > 0.05). Conclusions: PEI harbour a significantly higher number of F. alocis than those with SEI. Filifactor alocis was significantly associated with clinical features in primary endodontic infections. Total bacterial count was similar in both clinical conditions. |
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Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular studyBacteriaEndodonticsFilifactor alocisPersistent/secondary endodontic infectionPrimary endodontic infectionqPCRObjective: Identification of specific bacteria in root canals (RCs) in distinct clinical conditions can support the comprehension of pathological processes. Thus, the objective of this clinical study was to investigate the presence of F. alocis in RCs of teeth with primary endodontic infection (PEI) and with persistent/secondary endodontic infection (SEI) by using molecular techniques. It was also aimed to associate its presence with the clinical features. In addition, the levels of F. alocis as well as the total bacterial cells in the samples were also quantitated. Design: One hundred teeth (50 PEI and 50 SEI) were included. Microbial samples were performed using sterile paper points and assessed by using nested PCR and quantitative Real Time PCR (qPCR). The prevalence of F.alocis in RCs from PEI and SEI were compared by chi-square analysis. Fisher´s exact test or Pearson Chi-square, when appropriate, was used to test associations between clinical and radiographic features and the presence of F. alocis. Significance level was set at 5%. Results: F. alocis was detected in 23 and 28 (PEI) and 12 and 11 (SEI) RCs using Nested PCR and qPCR, respectively. Statistically significant associations were found between the presence of F. alocis and PEI, pain, wet canals, swelling, abscess and purulent exudate (P < 0.05). Total bacterial count was similar in both conditions (P > 0.05). Conclusions: PEI harbour a significantly higher number of F. alocis than those with SEI. Filifactor alocis was significantly associated with clinical features in primary endodontic infections. Total bacterial count was similar in both clinical conditions.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Restorative Dentistry Division of Endodontics Piracicaba Dental School State University of Campinas – UNICAMPFaculty of Medical Sciences University Center Lusíada - UNILUSDepartment of Operative Dentistry Division of Endodontics The University of São Paulo School of Dentistry - FOUSPDepartment of Semiology and Clinic Division of Endodontics School of Dentistry Federal University of Pelotas - UFPELDepartment of Endodontics Araçatuba School of Dentistry São Paulo State University - UNESPDepartment of Endodontics Araçatuba School of Dentistry São Paulo State University - UNESPFAPESP: 2015/23479-5FAPESP: 2017/25242-8FAPESP: 2019/19300-0Universidade Estadual de Campinas (UNICAMP)University Center Lusíada - UNILUSUniversidade de São Paulo (USP)Universidade Federal de Pernambuco (UFPE)Universidade Estadual Paulista (Unesp)Gomes, Brenda P.F.A.Louzada, Lidiane M.Almeida-Gomes, Rebecca F.Pinheiro, Ericka T.Sousa, Ezilmara L.R.Jacinto, Rogério C. [UNESP]Arruda-Vasconcelos, Rodrigo2020-12-12T02:15:29Z2020-12-12T02:15:29Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2020.104826Archives of Oral Biology, v. 118.1879-15060003-9969http://hdl.handle.net/11449/20076710.1016/j.archoralbio.2020.1048262-s2.0-8508810221839536300770470170000-0002-2362-8920Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2021-10-23T15:08:32Zoai:repositorio.unesp.br:11449/200767Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T15:08:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular study |
title |
Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular study |
spellingShingle |
Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular study Gomes, Brenda P.F.A. Bacteria Endodontics Filifactor alocis Persistent/secondary endodontic infection Primary endodontic infection qPCR |
title_short |
Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular study |
title_full |
Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular study |
title_fullStr |
Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular study |
title_full_unstemmed |
Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular study |
title_sort |
Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular study |
author |
Gomes, Brenda P.F.A. |
author_facet |
Gomes, Brenda P.F.A. Louzada, Lidiane M. Almeida-Gomes, Rebecca F. Pinheiro, Ericka T. Sousa, Ezilmara L.R. Jacinto, Rogério C. [UNESP] Arruda-Vasconcelos, Rodrigo |
author_role |
author |
author2 |
Louzada, Lidiane M. Almeida-Gomes, Rebecca F. Pinheiro, Ericka T. Sousa, Ezilmara L.R. Jacinto, Rogério C. [UNESP] Arruda-Vasconcelos, Rodrigo |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) University Center Lusíada - UNILUS Universidade de São Paulo (USP) Universidade Federal de Pernambuco (UFPE) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gomes, Brenda P.F.A. Louzada, Lidiane M. Almeida-Gomes, Rebecca F. Pinheiro, Ericka T. Sousa, Ezilmara L.R. Jacinto, Rogério C. [UNESP] Arruda-Vasconcelos, Rodrigo |
dc.subject.por.fl_str_mv |
Bacteria Endodontics Filifactor alocis Persistent/secondary endodontic infection Primary endodontic infection qPCR |
topic |
Bacteria Endodontics Filifactor alocis Persistent/secondary endodontic infection Primary endodontic infection qPCR |
description |
Objective: Identification of specific bacteria in root canals (RCs) in distinct clinical conditions can support the comprehension of pathological processes. Thus, the objective of this clinical study was to investigate the presence of F. alocis in RCs of teeth with primary endodontic infection (PEI) and with persistent/secondary endodontic infection (SEI) by using molecular techniques. It was also aimed to associate its presence with the clinical features. In addition, the levels of F. alocis as well as the total bacterial cells in the samples were also quantitated. Design: One hundred teeth (50 PEI and 50 SEI) were included. Microbial samples were performed using sterile paper points and assessed by using nested PCR and quantitative Real Time PCR (qPCR). The prevalence of F.alocis in RCs from PEI and SEI were compared by chi-square analysis. Fisher´s exact test or Pearson Chi-square, when appropriate, was used to test associations between clinical and radiographic features and the presence of F. alocis. Significance level was set at 5%. Results: F. alocis was detected in 23 and 28 (PEI) and 12 and 11 (SEI) RCs using Nested PCR and qPCR, respectively. Statistically significant associations were found between the presence of F. alocis and PEI, pain, wet canals, swelling, abscess and purulent exudate (P < 0.05). Total bacterial count was similar in both conditions (P > 0.05). Conclusions: PEI harbour a significantly higher number of F. alocis than those with SEI. Filifactor alocis was significantly associated with clinical features in primary endodontic infections. Total bacterial count was similar in both clinical conditions. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:15:29Z 2020-12-12T02:15:29Z 2020-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2020.104826 Archives of Oral Biology, v. 118. 1879-1506 0003-9969 http://hdl.handle.net/11449/200767 10.1016/j.archoralbio.2020.104826 2-s2.0-85088102218 3953630077047017 0000-0002-2362-8920 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2020.104826 http://hdl.handle.net/11449/200767 |
identifier_str_mv |
Archives of Oral Biology, v. 118. 1879-1506 0003-9969 10.1016/j.archoralbio.2020.104826 2-s2.0-85088102218 3953630077047017 0000-0002-2362-8920 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965639637467136 |