Antimicrobial peptides in saliva of children with severe early childhood caries
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2016.05.009 http://hdl.handle.net/11449/172975 |
Resumo: | Objective Controversies exist regarding the relationship between the concentrations of antimicrobial peptides (AMPs) and presence of dental caries in children. Thus, the aim of this study was to examine levels of AMPs in saliva of caries-free (CF), early childhood caries (ECC) and severe early childhood caries (S-ECC) children to determine if the levels of these salivary peptides individually or in combinations were related to caries severity and mutans streptococci levels. Design 36 to 60 month-old children were selected to participate in this study. Children were grouped into CF group (n = 29), ECC group (n = 25) and S-ECC group (n = 29). Saliva was collected from children for microbiological analysis by culture. Salivary concentrations of cathelicidin LL-37, human β-defensin 2 (hBD-2), human β-defensin 3 (hBD-3) and histatin-5 (HTN-5) were determined by ELISA. Results Salivary concentrations of AMPs did not differ among CF, ECC and S-ECC groups. Data showed positive correlations between mutans streptococci levels and salivary hBD-2 or HTN-5. Positive correlations were found between hBD-2, hBD-3, LL-37 and HTN-5. Combinations among AMPs, mainly LL-37, were positively associated with caries levels. Conclusions Salivary concentrations of AMPs individually were not associated with the severity of early childhood caries. The stimulus of caries appears to trigger a biological response, however, with a combination of these peptides. |
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Antimicrobial peptides in saliva of children with severe early childhood cariesChildrenDefensinsDental cariesHistatin-5Human cathelicidin LL-37Innate immunityObjective Controversies exist regarding the relationship between the concentrations of antimicrobial peptides (AMPs) and presence of dental caries in children. Thus, the aim of this study was to examine levels of AMPs in saliva of caries-free (CF), early childhood caries (ECC) and severe early childhood caries (S-ECC) children to determine if the levels of these salivary peptides individually or in combinations were related to caries severity and mutans streptococci levels. Design 36 to 60 month-old children were selected to participate in this study. Children were grouped into CF group (n = 29), ECC group (n = 25) and S-ECC group (n = 29). Saliva was collected from children for microbiological analysis by culture. Salivary concentrations of cathelicidin LL-37, human β-defensin 2 (hBD-2), human β-defensin 3 (hBD-3) and histatin-5 (HTN-5) were determined by ELISA. Results Salivary concentrations of AMPs did not differ among CF, ECC and S-ECC groups. Data showed positive correlations between mutans streptococci levels and salivary hBD-2 or HTN-5. Positive correlations were found between hBD-2, hBD-3, LL-37 and HTN-5. Combinations among AMPs, mainly LL-37, were positively associated with caries levels. Conclusions Salivary concentrations of AMPs individually were not associated with the severity of early childhood caries. The stimulus of caries appears to trigger a biological response, however, with a combination of these peptides.UNESP-Univ. Estadual Paulista Department of Pediatric Dentistry and Public Health Araçatuba Dental School, Rua José Bonifácio, 1193Forsyth Institute Department of MicrobiologyHarvard University Harvard School of Dental MedicineUNESP-Univ. Estadual Paulista Department of Pediatric Dentistry and Public Health Araçatuba Dental School, Rua José Bonifácio, 1193Universidade Estadual Paulista (Unesp)Forsyth InstituteHarvard School of Dental MedicineColombo, Natália H. [UNESP]Ribas, Laís F.F. [UNESP]Pereira, Jesse A. [UNESP]Kreling, Paula F. [UNESP]Kressirer, Christine A.Tanner, Anne C.R.Duque, Cristiane [UNESP]2018-12-11T17:02:58Z2018-12-11T17:02:58Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article40-46application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2016.05.009Archives of Oral Biology, v. 69, p. 40-46.1879-15060003-9969http://hdl.handle.net/11449/17297510.1016/j.archoralbio.2016.05.0092-s2.0-849697645112-s2.0-84969764511.pdf56518745094936170000-0002-2575-279XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2024-09-19T17:56:48Zoai:repositorio.unesp.br:11449/172975Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T17:56:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial peptides in saliva of children with severe early childhood caries |
title |
Antimicrobial peptides in saliva of children with severe early childhood caries |
spellingShingle |
Antimicrobial peptides in saliva of children with severe early childhood caries Colombo, Natália H. [UNESP] Children Defensins Dental caries Histatin-5 Human cathelicidin LL-37 Innate immunity |
title_short |
Antimicrobial peptides in saliva of children with severe early childhood caries |
title_full |
Antimicrobial peptides in saliva of children with severe early childhood caries |
title_fullStr |
Antimicrobial peptides in saliva of children with severe early childhood caries |
title_full_unstemmed |
Antimicrobial peptides in saliva of children with severe early childhood caries |
title_sort |
Antimicrobial peptides in saliva of children with severe early childhood caries |
author |
Colombo, Natália H. [UNESP] |
author_facet |
Colombo, Natália H. [UNESP] Ribas, Laís F.F. [UNESP] Pereira, Jesse A. [UNESP] Kreling, Paula F. [UNESP] Kressirer, Christine A. Tanner, Anne C.R. Duque, Cristiane [UNESP] |
author_role |
author |
author2 |
Ribas, Laís F.F. [UNESP] Pereira, Jesse A. [UNESP] Kreling, Paula F. [UNESP] Kressirer, Christine A. Tanner, Anne C.R. Duque, Cristiane [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Forsyth Institute Harvard School of Dental Medicine |
dc.contributor.author.fl_str_mv |
Colombo, Natália H. [UNESP] Ribas, Laís F.F. [UNESP] Pereira, Jesse A. [UNESP] Kreling, Paula F. [UNESP] Kressirer, Christine A. Tanner, Anne C.R. Duque, Cristiane [UNESP] |
dc.subject.por.fl_str_mv |
Children Defensins Dental caries Histatin-5 Human cathelicidin LL-37 Innate immunity |
topic |
Children Defensins Dental caries Histatin-5 Human cathelicidin LL-37 Innate immunity |
description |
Objective Controversies exist regarding the relationship between the concentrations of antimicrobial peptides (AMPs) and presence of dental caries in children. Thus, the aim of this study was to examine levels of AMPs in saliva of caries-free (CF), early childhood caries (ECC) and severe early childhood caries (S-ECC) children to determine if the levels of these salivary peptides individually or in combinations were related to caries severity and mutans streptococci levels. Design 36 to 60 month-old children were selected to participate in this study. Children were grouped into CF group (n = 29), ECC group (n = 25) and S-ECC group (n = 29). Saliva was collected from children for microbiological analysis by culture. Salivary concentrations of cathelicidin LL-37, human β-defensin 2 (hBD-2), human β-defensin 3 (hBD-3) and histatin-5 (HTN-5) were determined by ELISA. Results Salivary concentrations of AMPs did not differ among CF, ECC and S-ECC groups. Data showed positive correlations between mutans streptococci levels and salivary hBD-2 or HTN-5. Positive correlations were found between hBD-2, hBD-3, LL-37 and HTN-5. Combinations among AMPs, mainly LL-37, were positively associated with caries levels. Conclusions Salivary concentrations of AMPs individually were not associated with the severity of early childhood caries. The stimulus of caries appears to trigger a biological response, however, with a combination of these peptides. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2018-12-11T17:02:58Z 2018-12-11T17:02:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2016.05.009 Archives of Oral Biology, v. 69, p. 40-46. 1879-1506 0003-9969 http://hdl.handle.net/11449/172975 10.1016/j.archoralbio.2016.05.009 2-s2.0-84969764511 2-s2.0-84969764511.pdf 5651874509493617 0000-0002-2575-279X |
url |
http://dx.doi.org/10.1016/j.archoralbio.2016.05.009 http://hdl.handle.net/11449/172975 |
identifier_str_mv |
Archives of Oral Biology, v. 69, p. 40-46. 1879-1506 0003-9969 10.1016/j.archoralbio.2016.05.009 2-s2.0-84969764511 2-s2.0-84969764511.pdf 5651874509493617 0000-0002-2575-279X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
40-46 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546471716290560 |