Antimicrobial peptides in saliva of children with severe early childhood caries

Detalhes bibliográficos
Autor(a) principal: Colombo, Natália H. [UNESP]
Data de Publicação: 2016
Outros Autores: Ribas, Laís F.F. [UNESP], Pereira, Jesse A. [UNESP], Kreling, Paula F. [UNESP], Kressirer, Christine A., Tanner, Anne C.R., Duque, Cristiane [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2016.05.009
http://hdl.handle.net/11449/172975
Resumo: Objective Controversies exist regarding the relationship between the concentrations of antimicrobial peptides (AMPs) and presence of dental caries in children. Thus, the aim of this study was to examine levels of AMPs in saliva of caries-free (CF), early childhood caries (ECC) and severe early childhood caries (S-ECC) children to determine if the levels of these salivary peptides individually or in combinations were related to caries severity and mutans streptococci levels. Design 36 to 60 month-old children were selected to participate in this study. Children were grouped into CF group (n = 29), ECC group (n = 25) and S-ECC group (n = 29). Saliva was collected from children for microbiological analysis by culture. Salivary concentrations of cathelicidin LL-37, human β-defensin 2 (hBD-2), human β-defensin 3 (hBD-3) and histatin-5 (HTN-5) were determined by ELISA. Results Salivary concentrations of AMPs did not differ among CF, ECC and S-ECC groups. Data showed positive correlations between mutans streptococci levels and salivary hBD-2 or HTN-5. Positive correlations were found between hBD-2, hBD-3, LL-37 and HTN-5. Combinations among AMPs, mainly LL-37, were positively associated with caries levels. Conclusions Salivary concentrations of AMPs individually were not associated with the severity of early childhood caries. The stimulus of caries appears to trigger a biological response, however, with a combination of these peptides.
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spelling Antimicrobial peptides in saliva of children with severe early childhood cariesChildrenDefensinsDental cariesHistatin-5Human cathelicidin LL-37Innate immunityObjective Controversies exist regarding the relationship between the concentrations of antimicrobial peptides (AMPs) and presence of dental caries in children. Thus, the aim of this study was to examine levels of AMPs in saliva of caries-free (CF), early childhood caries (ECC) and severe early childhood caries (S-ECC) children to determine if the levels of these salivary peptides individually or in combinations were related to caries severity and mutans streptococci levels. Design 36 to 60 month-old children were selected to participate in this study. Children were grouped into CF group (n = 29), ECC group (n = 25) and S-ECC group (n = 29). Saliva was collected from children for microbiological analysis by culture. Salivary concentrations of cathelicidin LL-37, human β-defensin 2 (hBD-2), human β-defensin 3 (hBD-3) and histatin-5 (HTN-5) were determined by ELISA. Results Salivary concentrations of AMPs did not differ among CF, ECC and S-ECC groups. Data showed positive correlations between mutans streptococci levels and salivary hBD-2 or HTN-5. Positive correlations were found between hBD-2, hBD-3, LL-37 and HTN-5. Combinations among AMPs, mainly LL-37, were positively associated with caries levels. Conclusions Salivary concentrations of AMPs individually were not associated with the severity of early childhood caries. The stimulus of caries appears to trigger a biological response, however, with a combination of these peptides.UNESP-Univ. Estadual Paulista Department of Pediatric Dentistry and Public Health Araçatuba Dental School, Rua José Bonifácio, 1193Forsyth Institute Department of MicrobiologyHarvard University Harvard School of Dental MedicineUNESP-Univ. Estadual Paulista Department of Pediatric Dentistry and Public Health Araçatuba Dental School, Rua José Bonifácio, 1193Universidade Estadual Paulista (Unesp)Forsyth InstituteHarvard School of Dental MedicineColombo, Natália H. [UNESP]Ribas, Laís F.F. [UNESP]Pereira, Jesse A. [UNESP]Kreling, Paula F. [UNESP]Kressirer, Christine A.Tanner, Anne C.R.Duque, Cristiane [UNESP]2018-12-11T17:02:58Z2018-12-11T17:02:58Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article40-46application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2016.05.009Archives of Oral Biology, v. 69, p. 40-46.1879-15060003-9969http://hdl.handle.net/11449/17297510.1016/j.archoralbio.2016.05.0092-s2.0-849697645112-s2.0-84969764511.pdf56518745094936170000-0002-2575-279XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2024-09-19T17:56:48Zoai:repositorio.unesp.br:11449/172975Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T17:56:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antimicrobial peptides in saliva of children with severe early childhood caries
title Antimicrobial peptides in saliva of children with severe early childhood caries
spellingShingle Antimicrobial peptides in saliva of children with severe early childhood caries
Colombo, Natália H. [UNESP]
Children
Defensins
Dental caries
Histatin-5
Human cathelicidin LL-37
Innate immunity
title_short Antimicrobial peptides in saliva of children with severe early childhood caries
title_full Antimicrobial peptides in saliva of children with severe early childhood caries
title_fullStr Antimicrobial peptides in saliva of children with severe early childhood caries
title_full_unstemmed Antimicrobial peptides in saliva of children with severe early childhood caries
title_sort Antimicrobial peptides in saliva of children with severe early childhood caries
author Colombo, Natália H. [UNESP]
author_facet Colombo, Natália H. [UNESP]
Ribas, Laís F.F. [UNESP]
Pereira, Jesse A. [UNESP]
Kreling, Paula F. [UNESP]
Kressirer, Christine A.
Tanner, Anne C.R.
Duque, Cristiane [UNESP]
author_role author
author2 Ribas, Laís F.F. [UNESP]
Pereira, Jesse A. [UNESP]
Kreling, Paula F. [UNESP]
Kressirer, Christine A.
Tanner, Anne C.R.
Duque, Cristiane [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Forsyth Institute
Harvard School of Dental Medicine
dc.contributor.author.fl_str_mv Colombo, Natália H. [UNESP]
Ribas, Laís F.F. [UNESP]
Pereira, Jesse A. [UNESP]
Kreling, Paula F. [UNESP]
Kressirer, Christine A.
Tanner, Anne C.R.
Duque, Cristiane [UNESP]
dc.subject.por.fl_str_mv Children
Defensins
Dental caries
Histatin-5
Human cathelicidin LL-37
Innate immunity
topic Children
Defensins
Dental caries
Histatin-5
Human cathelicidin LL-37
Innate immunity
description Objective Controversies exist regarding the relationship between the concentrations of antimicrobial peptides (AMPs) and presence of dental caries in children. Thus, the aim of this study was to examine levels of AMPs in saliva of caries-free (CF), early childhood caries (ECC) and severe early childhood caries (S-ECC) children to determine if the levels of these salivary peptides individually or in combinations were related to caries severity and mutans streptococci levels. Design 36 to 60 month-old children were selected to participate in this study. Children were grouped into CF group (n = 29), ECC group (n = 25) and S-ECC group (n = 29). Saliva was collected from children for microbiological analysis by culture. Salivary concentrations of cathelicidin LL-37, human β-defensin 2 (hBD-2), human β-defensin 3 (hBD-3) and histatin-5 (HTN-5) were determined by ELISA. Results Salivary concentrations of AMPs did not differ among CF, ECC and S-ECC groups. Data showed positive correlations between mutans streptococci levels and salivary hBD-2 or HTN-5. Positive correlations were found between hBD-2, hBD-3, LL-37 and HTN-5. Combinations among AMPs, mainly LL-37, were positively associated with caries levels. Conclusions Salivary concentrations of AMPs individually were not associated with the severity of early childhood caries. The stimulus of caries appears to trigger a biological response, however, with a combination of these peptides.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
2018-12-11T17:02:58Z
2018-12-11T17:02:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2016.05.009
Archives of Oral Biology, v. 69, p. 40-46.
1879-1506
0003-9969
http://hdl.handle.net/11449/172975
10.1016/j.archoralbio.2016.05.009
2-s2.0-84969764511
2-s2.0-84969764511.pdf
5651874509493617
0000-0002-2575-279X
url http://dx.doi.org/10.1016/j.archoralbio.2016.05.009
http://hdl.handle.net/11449/172975
identifier_str_mv Archives of Oral Biology, v. 69, p. 40-46.
1879-1506
0003-9969
10.1016/j.archoralbio.2016.05.009
2-s2.0-84969764511
2-s2.0-84969764511.pdf
5651874509493617
0000-0002-2575-279X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Oral Biology
0,752
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 40-46
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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