Development of an Antibacterial Dentin Adhesive
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/polym14122502 http://hdl.handle.net/11449/240355 |
Resumo: | Nisin is a peptide that possesses potent antibacterial properties. This study evaluated the antibacterial activity of a nisin-doped adhesive against Streptococcus mutans, as well as its degree of conversion and microtensile bond strength (µTBS) to dentin. Nisin was added to the adhesive Adper Single Bond 2 (3M ESPE), resulting in four groups: Control Group (Single Bond 2); Group 1% (1 wt% nisin-incorporated), Group 3% (3 wt% nisin-incorporated) and Group 5% (5 wt% nisin-incorporated). Antibacterial activity against S. mutans was evaluated using colony-forming unit counts (CFU). The degree of conversion was tested using FTIR. Forty human teeth were restored for µTBS evaluation. Data were statistically analyzed with ANOVA and Tukey tests at α = 0.05. The nisin-doped adhesives, for all concentrations, exhibited a significant inhibition of the growth of S. mutans (p < 0.05); Incorporation of 5% and 3% nisin decreased the degree of conversion of the adhesive (p < 0.05). The µTBS (in MPa): Control Group—38.3 ± 2.3A, Group 1%—35.6 ± 2.1A, Group 3%—27.1 ± 1.6B and Group 5%—22.3 ± 1.0C. Nisin-doped adhesives exerted a bactericidal effect on S. mutans. The µTBS and degree of conversion of adhesive were not affected after incorporation of 1% nisin. |
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Development of an Antibacterial Dentin Adhesiveantibacterial activitydentinetch-and-rinse adhesivenisinNisin is a peptide that possesses potent antibacterial properties. This study evaluated the antibacterial activity of a nisin-doped adhesive against Streptococcus mutans, as well as its degree of conversion and microtensile bond strength (µTBS) to dentin. Nisin was added to the adhesive Adper Single Bond 2 (3M ESPE), resulting in four groups: Control Group (Single Bond 2); Group 1% (1 wt% nisin-incorporated), Group 3% (3 wt% nisin-incorporated) and Group 5% (5 wt% nisin-incorporated). Antibacterial activity against S. mutans was evaluated using colony-forming unit counts (CFU). The degree of conversion was tested using FTIR. Forty human teeth were restored for µTBS evaluation. Data were statistically analyzed with ANOVA and Tukey tests at α = 0.05. The nisin-doped adhesives, for all concentrations, exhibited a significant inhibition of the growth of S. mutans (p < 0.05); Incorporation of 5% and 3% nisin decreased the degree of conversion of the adhesive (p < 0.05). The µTBS (in MPa): Control Group—38.3 ± 2.3A, Group 1%—35.6 ± 2.1A, Group 3%—27.1 ± 1.6B and Group 5%—22.3 ± 1.0C. Nisin-doped adhesives exerted a bactericidal effect on S. mutans. The µTBS and degree of conversion of adhesive were not affected after incorporation of 1% nisin.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (UNESP), São PauloDepartment of Prosthodontics Federal University of Espírito Santo, Espírito SantoState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology Department of Prosthodontics School of Stomatology The Fourth Military Medical UniversityDepartment of Endodontics The Dental College of Georgia Augusta UniversityDepartment of Restorative Dentistry Institute of Science and Technology São Paulo State University (UNESP), São PauloFAPESP: 2019/01626-7Universidade Estadual Paulista (UNESP)Federal University of Espírito SantoThe Fourth Military Medical UniversityAugusta UniversityLopes, Stephanie R. [UNESP]Matuda, Amanda G. N. [UNESP]Campos, Raquel P. [UNESP]Mafetano, Ana Paula V. P. [UNESP]Barnabe, Ana Helena M. [UNESP]Chagas, Gabriela S. [UNESP]Barcellos, Daphne C.Niu, Li-NaTay, Franklin R.Pucci, Cesar R. [UNESP]2023-03-01T20:13:25Z2023-03-01T20:13:25Z2022-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/polym14122502Polymers, v. 14, n. 12, 2022.2073-4360http://hdl.handle.net/11449/24035510.3390/polym141225022-s2.0-85132892566Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPolymersinfo:eu-repo/semantics/openAccess2023-03-01T20:13:26Zoai:repositorio.unesp.br:11449/240355Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:14:19.786742Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development of an Antibacterial Dentin Adhesive |
title |
Development of an Antibacterial Dentin Adhesive |
spellingShingle |
Development of an Antibacterial Dentin Adhesive Lopes, Stephanie R. [UNESP] antibacterial activity dentin etch-and-rinse adhesive nisin |
title_short |
Development of an Antibacterial Dentin Adhesive |
title_full |
Development of an Antibacterial Dentin Adhesive |
title_fullStr |
Development of an Antibacterial Dentin Adhesive |
title_full_unstemmed |
Development of an Antibacterial Dentin Adhesive |
title_sort |
Development of an Antibacterial Dentin Adhesive |
author |
Lopes, Stephanie R. [UNESP] |
author_facet |
Lopes, Stephanie R. [UNESP] Matuda, Amanda G. N. [UNESP] Campos, Raquel P. [UNESP] Mafetano, Ana Paula V. P. [UNESP] Barnabe, Ana Helena M. [UNESP] Chagas, Gabriela S. [UNESP] Barcellos, Daphne C. Niu, Li-Na Tay, Franklin R. Pucci, Cesar R. [UNESP] |
author_role |
author |
author2 |
Matuda, Amanda G. N. [UNESP] Campos, Raquel P. [UNESP] Mafetano, Ana Paula V. P. [UNESP] Barnabe, Ana Helena M. [UNESP] Chagas, Gabriela S. [UNESP] Barcellos, Daphne C. Niu, Li-Na Tay, Franklin R. Pucci, Cesar R. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Federal University of Espírito Santo The Fourth Military Medical University Augusta University |
dc.contributor.author.fl_str_mv |
Lopes, Stephanie R. [UNESP] Matuda, Amanda G. N. [UNESP] Campos, Raquel P. [UNESP] Mafetano, Ana Paula V. P. [UNESP] Barnabe, Ana Helena M. [UNESP] Chagas, Gabriela S. [UNESP] Barcellos, Daphne C. Niu, Li-Na Tay, Franklin R. Pucci, Cesar R. [UNESP] |
dc.subject.por.fl_str_mv |
antibacterial activity dentin etch-and-rinse adhesive nisin |
topic |
antibacterial activity dentin etch-and-rinse adhesive nisin |
description |
Nisin is a peptide that possesses potent antibacterial properties. This study evaluated the antibacterial activity of a nisin-doped adhesive against Streptococcus mutans, as well as its degree of conversion and microtensile bond strength (µTBS) to dentin. Nisin was added to the adhesive Adper Single Bond 2 (3M ESPE), resulting in four groups: Control Group (Single Bond 2); Group 1% (1 wt% nisin-incorporated), Group 3% (3 wt% nisin-incorporated) and Group 5% (5 wt% nisin-incorporated). Antibacterial activity against S. mutans was evaluated using colony-forming unit counts (CFU). The degree of conversion was tested using FTIR. Forty human teeth were restored for µTBS evaluation. Data were statistically analyzed with ANOVA and Tukey tests at α = 0.05. The nisin-doped adhesives, for all concentrations, exhibited a significant inhibition of the growth of S. mutans (p < 0.05); Incorporation of 5% and 3% nisin decreased the degree of conversion of the adhesive (p < 0.05). The µTBS (in MPa): Control Group—38.3 ± 2.3A, Group 1%—35.6 ± 2.1A, Group 3%—27.1 ± 1.6B and Group 5%—22.3 ± 1.0C. Nisin-doped adhesives exerted a bactericidal effect on S. mutans. The µTBS and degree of conversion of adhesive were not affected after incorporation of 1% nisin. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-01 2023-03-01T20:13:25Z 2023-03-01T20:13:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/polym14122502 Polymers, v. 14, n. 12, 2022. 2073-4360 http://hdl.handle.net/11449/240355 10.3390/polym14122502 2-s2.0-85132892566 |
url |
http://dx.doi.org/10.3390/polym14122502 http://hdl.handle.net/11449/240355 |
identifier_str_mv |
Polymers, v. 14, n. 12, 2022. 2073-4360 10.3390/polym14122502 2-s2.0-85132892566 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Polymers |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129598698815488 |