Investigation on electrochemical behavior of peroxidase enzyme in the presence of hydrogen peroxide and 5-aminosalicylic acid

• Autor(a) principal: Uliana, C. V. [UNESP]
• Data de Publicação: 2008
• Outros Autores: Riccardi, C. S. [UNESP], Yamanaka, H. [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.26850/1678-4618EQJ.V33.1.2008.P57-62; http://hdl.handle.net/11449/222606
• topic: 5-aminosalicilic acid; Graphite electrode; Hydrogen peroxide; Peroxidase

The electrochemical behavior of the enzyme peroxidase (HRP) was investigated using the hydrogen peroxide as enzymatic substrate and the 5-aminosalicylic acid (5-ASA) as mediator of electrons on graphite electrodes. Several parameters were optimized, namely, the applied potential to the amperometric technique fixed in -0.125V, the 0.1 mol L-1phosphate-citrate buffer at pH 5.0 as supporting electrolyte and the proportion between the 5-ASA and H2O2in 1:7, among others. It was observed the catalysis of the oxidation reaction of the H2O2in the presence of the enzyme HRP and 5- ASA. The oxidation product was reduced in the electrode surface, evidencing a significant increase in the intensity of the cathodic current.