Effect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injury
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pupt.2020.101896 http://hdl.handle.net/11449/200010 |
Resumo: | Chronic obstructive pulmonary disease (COPD) is related to smoking and anti-inflammatory therapy is indicated. Among the mediators with anti-inflammatory properties, we highlight piperlongumine (PL), an alkaloid/amide of Piper longum. Here we evaluated the PL administration on an experimental model of respiratory inflammation resulting from exposure to cigarette smoke. Male Balb/c mice were exposed to burning of 10 commercial cigarettes, 2x/day, for five weeks on specific equipment. PL efficacy was evaluated in control, exposed to smoke without treatment and PL treated (2.0 mg/kg, 3x/week) groups. Animals were weighed and plethysmographic analyses performed at the end of the exposure protocol. Inflammatory cells were evaluated in the bronchoalveolar lavage (BAL) and hemoglobin and glucose in the blood. Lung fragments were processed for histopathological studies and AnxA1, COX-2, NF-kB and neutrophil elastase expressions. Plethysmography revealed that PL maintained pulmonary frequency, volume and ventilation parameters similar to controls, with respiratory volume reduction compared to untreated animals. Final weight was reduced in both exposed groups. PL decreased hemoglobin concentration, attenuated the reduction of glucose levels and reduced influx of lymphocytes, neutrophils and macrophages in BAL. Histopathologically occured infiltration of inflammatory cells, increase of the interalveolar septa and intra-alveolar spaces in untreated animals. But, PL administration recovered lung tissues and, immunohistochemically, promoted increased expression of AnxA1 and reduction of COX-2, NF-kB and neutrophil elastase. Together the results indicate that PL attenuates systemic and pulmonary inflammatory changes, partially by modulating the expression the endogenous AnxA1, and may represent a promising therapy in preventing the inflammation induced by cigarette smoke. |
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Effect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injuryAnnexin A1Bronchoalveolar lavageChronic obstructive pulmonary diseaseInflammatory cellsMiceSmokingChronic obstructive pulmonary disease (COPD) is related to smoking and anti-inflammatory therapy is indicated. Among the mediators with anti-inflammatory properties, we highlight piperlongumine (PL), an alkaloid/amide of Piper longum. Here we evaluated the PL administration on an experimental model of respiratory inflammation resulting from exposure to cigarette smoke. Male Balb/c mice were exposed to burning of 10 commercial cigarettes, 2x/day, for five weeks on specific equipment. PL efficacy was evaluated in control, exposed to smoke without treatment and PL treated (2.0 mg/kg, 3x/week) groups. Animals were weighed and plethysmographic analyses performed at the end of the exposure protocol. Inflammatory cells were evaluated in the bronchoalveolar lavage (BAL) and hemoglobin and glucose in the blood. Lung fragments were processed for histopathological studies and AnxA1, COX-2, NF-kB and neutrophil elastase expressions. Plethysmography revealed that PL maintained pulmonary frequency, volume and ventilation parameters similar to controls, with respiratory volume reduction compared to untreated animals. Final weight was reduced in both exposed groups. PL decreased hemoglobin concentration, attenuated the reduction of glucose levels and reduced influx of lymphocytes, neutrophils and macrophages in BAL. Histopathologically occured infiltration of inflammatory cells, increase of the interalveolar septa and intra-alveolar spaces in untreated animals. But, PL administration recovered lung tissues and, immunohistochemically, promoted increased expression of AnxA1 and reduction of COX-2, NF-kB and neutrophil elastase. Together the results indicate that PL attenuates systemic and pulmonary inflammatory changes, partially by modulating the expression the endogenous AnxA1, and may represent a promising therapy in preventing the inflammation induced by cigarette smoke.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Morphology and Genetics São Paulo Federal University (UNIFESP)University Center Padre Albino (UNIFIPA)Department of Physics São Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences (IBILCE), São José Do Rio Preto CampusSackler Institute of Pulmonary Pharmacology Institute of Pharmaceutical Science King's College LondonDepartment of Biology Laboratory of Immunomorphology São Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences (IBILCE), São José Do Rio Preto CampusDepartment of Physics São Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences (IBILCE), São José Do Rio Preto CampusDepartment of Biology Laboratory of Immunomorphology São Paulo State University (UNESP) Institute of Biosciences Humanities and Exact Sciences (IBILCE), São José Do Rio Preto CampusFAPESP: 2012/13041-4FAPESP: 2016/02012-4CNPq: 308144/2014-7CNPq: 404190/2016-2Universidade Federal de São Paulo (UNIFESP)University Center Padre Albino (UNIFIPA)Universidade Estadual Paulista (Unesp)King's College LondonSant'Ana, MonielleSouza, Helena R. [UNESP]Possebon, Lucas [UNESP]Cornélio, Marinônio L. [UNESP]Riffo-Vasquez, YaniraGirol, Ana Paula [UNESP]Oliani, Sonia M. [UNESP]2020-12-12T01:55:13Z2020-12-12T01:55:13Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pupt.2020.101896Pulmonary Pharmacology and Therapeutics, v. 61.1522-96291094-5539http://hdl.handle.net/11449/20001010.1016/j.pupt.2020.1018962-s2.0-85078662244Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPulmonary Pharmacology and Therapeuticsinfo:eu-repo/semantics/openAccess2021-10-23T10:41:20Zoai:repositorio.unesp.br:11449/200010Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:39:56.641160Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injury |
title |
Effect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injury |
spellingShingle |
Effect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injury Sant'Ana, Monielle Annexin A1 Bronchoalveolar lavage Chronic obstructive pulmonary disease Inflammatory cells Mice Smoking |
title_short |
Effect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injury |
title_full |
Effect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injury |
title_fullStr |
Effect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injury |
title_full_unstemmed |
Effect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injury |
title_sort |
Effect of piperlongumine during exposure to cigarette smoke reduces inflammation and lung injury |
author |
Sant'Ana, Monielle |
author_facet |
Sant'Ana, Monielle Souza, Helena R. [UNESP] Possebon, Lucas [UNESP] Cornélio, Marinônio L. [UNESP] Riffo-Vasquez, Yanira Girol, Ana Paula [UNESP] Oliani, Sonia M. [UNESP] |
author_role |
author |
author2 |
Souza, Helena R. [UNESP] Possebon, Lucas [UNESP] Cornélio, Marinônio L. [UNESP] Riffo-Vasquez, Yanira Girol, Ana Paula [UNESP] Oliani, Sonia M. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) University Center Padre Albino (UNIFIPA) Universidade Estadual Paulista (Unesp) King's College London |
dc.contributor.author.fl_str_mv |
Sant'Ana, Monielle Souza, Helena R. [UNESP] Possebon, Lucas [UNESP] Cornélio, Marinônio L. [UNESP] Riffo-Vasquez, Yanira Girol, Ana Paula [UNESP] Oliani, Sonia M. [UNESP] |
dc.subject.por.fl_str_mv |
Annexin A1 Bronchoalveolar lavage Chronic obstructive pulmonary disease Inflammatory cells Mice Smoking |
topic |
Annexin A1 Bronchoalveolar lavage Chronic obstructive pulmonary disease Inflammatory cells Mice Smoking |
description |
Chronic obstructive pulmonary disease (COPD) is related to smoking and anti-inflammatory therapy is indicated. Among the mediators with anti-inflammatory properties, we highlight piperlongumine (PL), an alkaloid/amide of Piper longum. Here we evaluated the PL administration on an experimental model of respiratory inflammation resulting from exposure to cigarette smoke. Male Balb/c mice were exposed to burning of 10 commercial cigarettes, 2x/day, for five weeks on specific equipment. PL efficacy was evaluated in control, exposed to smoke without treatment and PL treated (2.0 mg/kg, 3x/week) groups. Animals were weighed and plethysmographic analyses performed at the end of the exposure protocol. Inflammatory cells were evaluated in the bronchoalveolar lavage (BAL) and hemoglobin and glucose in the blood. Lung fragments were processed for histopathological studies and AnxA1, COX-2, NF-kB and neutrophil elastase expressions. Plethysmography revealed that PL maintained pulmonary frequency, volume and ventilation parameters similar to controls, with respiratory volume reduction compared to untreated animals. Final weight was reduced in both exposed groups. PL decreased hemoglobin concentration, attenuated the reduction of glucose levels and reduced influx of lymphocytes, neutrophils and macrophages in BAL. Histopathologically occured infiltration of inflammatory cells, increase of the interalveolar septa and intra-alveolar spaces in untreated animals. But, PL administration recovered lung tissues and, immunohistochemically, promoted increased expression of AnxA1 and reduction of COX-2, NF-kB and neutrophil elastase. Together the results indicate that PL attenuates systemic and pulmonary inflammatory changes, partially by modulating the expression the endogenous AnxA1, and may represent a promising therapy in preventing the inflammation induced by cigarette smoke. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:55:13Z 2020-12-12T01:55:13Z 2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pupt.2020.101896 Pulmonary Pharmacology and Therapeutics, v. 61. 1522-9629 1094-5539 http://hdl.handle.net/11449/200010 10.1016/j.pupt.2020.101896 2-s2.0-85078662244 |
url |
http://dx.doi.org/10.1016/j.pupt.2020.101896 http://hdl.handle.net/11449/200010 |
identifier_str_mv |
Pulmonary Pharmacology and Therapeutics, v. 61. 1522-9629 1094-5539 10.1016/j.pupt.2020.101896 2-s2.0-85078662244 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pulmonary Pharmacology and Therapeutics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128547678584832 |