Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminium
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1071/FP19032 http://hdl.handle.net/11449/201431 |
Resumo: | In acidic soils, aluminium (Al) occurs as Al3+, which is phytotoxic. One of the most conspicuous symptoms of Al toxicity is the root growth inhibition, which can lead to low water uptake and consequent reduction in leaf hydration and gas exchange. However, fibrous xylem vessels have been observed in roots of 'Rangpur' lime plants (Citrus limonia L.) when exposed to Al, which could affect the functioning of aquaporins, ultimately reducing their expression. We confirmed a decrease of CO2 assimilation (A), stomatal conductance (gs), transpiration (E) and relative leaf water content (RWC) in 3-month-old C. limonia plants exposed to 1480 μM Al in nutrient solution for 90 days. The estimated hydraulic conductivity from soil to the leaf (KL) and leaf water potential (Ψw) also showed low values, although not consistently reduced over time of Al exposure. The relative expression of aquaporin genes belonging to PIP family (PIP1-1, PIP1-2 and PIP2) showed downregulation for ClPIP1-1 and ClPIP2 and upregulation for ClPIP1-2 in plants exposed to Al. Furthermore, ClPIP1-1 was positively correlated with A and gs in plants exposed to Al. Therefore, downregulation of ClPIP1-1 and ClPIP2 in roots of 'Rangpur' lime plants could be associated with the low leaf hydration of this species when exposed to Al. |
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Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminiumabiotic stressCitrus limoniaPIPqPCRwater relationsIn acidic soils, aluminium (Al) occurs as Al3+, which is phytotoxic. One of the most conspicuous symptoms of Al toxicity is the root growth inhibition, which can lead to low water uptake and consequent reduction in leaf hydration and gas exchange. However, fibrous xylem vessels have been observed in roots of 'Rangpur' lime plants (Citrus limonia L.) when exposed to Al, which could affect the functioning of aquaporins, ultimately reducing their expression. We confirmed a decrease of CO2 assimilation (A), stomatal conductance (gs), transpiration (E) and relative leaf water content (RWC) in 3-month-old C. limonia plants exposed to 1480 μM Al in nutrient solution for 90 days. The estimated hydraulic conductivity from soil to the leaf (KL) and leaf water potential (Ψw) also showed low values, although not consistently reduced over time of Al exposure. The relative expression of aquaporin genes belonging to PIP family (PIP1-1, PIP1-2 and PIP2) showed downregulation for ClPIP1-1 and ClPIP2 and upregulation for ClPIP1-2 in plants exposed to Al. Furthermore, ClPIP1-1 was positively correlated with A and gs in plants exposed to Al. Therefore, downregulation of ClPIP1-1 and ClPIP2 in roots of 'Rangpur' lime plants could be associated with the low leaf hydration of this species when exposed to Al.Programa de Pós-Graduacąõ em Ciências Biológicas (Biologia Vegetal) Universidade Estadual Paulista UNESP Instituto de Biociências Departamento de Botânica, Av. 24-A, 1515Escola Superior de Agricultura 'Luiz de Queiróz' Universidade de Saõ Paulo ESALQ-USP Departamento de Ciências Biológicas, Av. Pádua Dias, 11Departamento de Botânica Universidade Estadual Paulista UNESP Instituto de Biociências, Av. 24-A, 1515Programa de Pós-Graduacąõ em Ciências Biológicas (Biologia Vegetal) Universidade Estadual Paulista UNESP Instituto de Biociências Departamento de Botânica, Av. 24-A, 1515Departamento de Botânica Universidade Estadual Paulista UNESP Instituto de Biociências, Av. 24-A, 1515Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Cavalheiro, Mariana F. [UNESP]Gavassi, Marina A. [UNESP]Silva, Giselle S. [UNESP]Nogueira, Matheus A. [UNESP]Silva, Carolina M. S.Domingues, Douglas S. [UNESP]Habermann, Gustavo [UNESP]2020-12-12T02:32:21Z2020-12-12T02:32:21Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article112-121http://dx.doi.org/10.1071/FP19032Functional Plant Biology, v. 47, n. 2, p. 112-121, 2020.1445-44161445-4408http://hdl.handle.net/11449/20143110.1071/FP190322-s2.0-85077063603Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFunctional Plant Biologyinfo:eu-repo/semantics/openAccess2021-10-22T19:03:26Zoai:repositorio.unesp.br:11449/201431Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:30:42.060137Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminium |
title |
Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminium |
spellingShingle |
Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminium Cavalheiro, Mariana F. [UNESP] abiotic stress Citrus limonia PIP qPCR water relations |
title_short |
Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminium |
title_full |
Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminium |
title_fullStr |
Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminium |
title_full_unstemmed |
Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminium |
title_sort |
Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminium |
author |
Cavalheiro, Mariana F. [UNESP] |
author_facet |
Cavalheiro, Mariana F. [UNESP] Gavassi, Marina A. [UNESP] Silva, Giselle S. [UNESP] Nogueira, Matheus A. [UNESP] Silva, Carolina M. S. Domingues, Douglas S. [UNESP] Habermann, Gustavo [UNESP] |
author_role |
author |
author2 |
Gavassi, Marina A. [UNESP] Silva, Giselle S. [UNESP] Nogueira, Matheus A. [UNESP] Silva, Carolina M. S. Domingues, Douglas S. [UNESP] Habermann, Gustavo [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Cavalheiro, Mariana F. [UNESP] Gavassi, Marina A. [UNESP] Silva, Giselle S. [UNESP] Nogueira, Matheus A. [UNESP] Silva, Carolina M. S. Domingues, Douglas S. [UNESP] Habermann, Gustavo [UNESP] |
dc.subject.por.fl_str_mv |
abiotic stress Citrus limonia PIP qPCR water relations |
topic |
abiotic stress Citrus limonia PIP qPCR water relations |
description |
In acidic soils, aluminium (Al) occurs as Al3+, which is phytotoxic. One of the most conspicuous symptoms of Al toxicity is the root growth inhibition, which can lead to low water uptake and consequent reduction in leaf hydration and gas exchange. However, fibrous xylem vessels have been observed in roots of 'Rangpur' lime plants (Citrus limonia L.) when exposed to Al, which could affect the functioning of aquaporins, ultimately reducing their expression. We confirmed a decrease of CO2 assimilation (A), stomatal conductance (gs), transpiration (E) and relative leaf water content (RWC) in 3-month-old C. limonia plants exposed to 1480 μM Al in nutrient solution for 90 days. The estimated hydraulic conductivity from soil to the leaf (KL) and leaf water potential (Ψw) also showed low values, although not consistently reduced over time of Al exposure. The relative expression of aquaporin genes belonging to PIP family (PIP1-1, PIP1-2 and PIP2) showed downregulation for ClPIP1-1 and ClPIP2 and upregulation for ClPIP1-2 in plants exposed to Al. Furthermore, ClPIP1-1 was positively correlated with A and gs in plants exposed to Al. Therefore, downregulation of ClPIP1-1 and ClPIP2 in roots of 'Rangpur' lime plants could be associated with the low leaf hydration of this species when exposed to Al. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:32:21Z 2020-12-12T02:32:21Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1071/FP19032 Functional Plant Biology, v. 47, n. 2, p. 112-121, 2020. 1445-4416 1445-4408 http://hdl.handle.net/11449/201431 10.1071/FP19032 2-s2.0-85077063603 |
url |
http://dx.doi.org/10.1071/FP19032 http://hdl.handle.net/11449/201431 |
identifier_str_mv |
Functional Plant Biology, v. 47, n. 2, p. 112-121, 2020. 1445-4416 1445-4408 10.1071/FP19032 2-s2.0-85077063603 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Functional Plant Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
112-121 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128819583778816 |