Lack of Population Genetic Structuring in Ocelots (Leopardus pardalis) in a Fragmented Landscape
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/d7030295 http://hdl.handle.net/11449/220484 |
Resumo: | Habitat fragmentation can promote patches of small and isolated populations, gene flow disruption between those populations, and reduction of local and total genetic variation. As a consequence, these small populations may go extinct in the long-term. The ocelot (Leopardus pardalis), originally distributed from Texas to southern Brazil and northern Argentina, has been impacted by habitat fragmentation throughout much of its range. To test whether habitat fragmentation has already induced genetic differentiation in an area where this process has been documented for a larger felid (jaguars), we analyzed molecular variation in ocelots inhabiting two Atlantic Forest fragments, Morro do Diabo (MD) and Iguaçu Region (IR). Analyses using nine microsatellites revealed mean observed and expected heterozygosity of 0.68 and 0.70, respectively. The MD sampled population showed evidence of a genetic bottleneck under two mutational models (TPM = 0.03711 and SMM = 0.04883). Estimates of genetic structure (FST = 0.027; best fit of k = 1 with STRUCTURE) revealed no meaningful differentiation between these populations. Thus, our results indicate that the ocelot populations sampled in these fragments are still not significantly different genetically, a pattern that strongly contrasts with that previously observed in jaguars for the same comparisons. This observation is likely due to a combination of two factors: (i) larger effective population size of ocelots (relative to jaguars) in each fragment, implying a slower effect of drift-induced differentiation; and (ii) potentially some remaining permeability of the anthropogenic matrix for ocelots, as opposed to the observed lack of permeability for jaguars. The persistence of ocelot gene flow between these areas must be prioritized in long-term conservation planning on behalf of these felids. |
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Lack of Population Genetic Structuring in Ocelots (Leopardus pardalis) in a Fragmented LandscapeBottleneckFelidGenetic diversityHabitat fragmentationHabitat fragmentation can promote patches of small and isolated populations, gene flow disruption between those populations, and reduction of local and total genetic variation. As a consequence, these small populations may go extinct in the long-term. The ocelot (Leopardus pardalis), originally distributed from Texas to southern Brazil and northern Argentina, has been impacted by habitat fragmentation throughout much of its range. To test whether habitat fragmentation has already induced genetic differentiation in an area where this process has been documented for a larger felid (jaguars), we analyzed molecular variation in ocelots inhabiting two Atlantic Forest fragments, Morro do Diabo (MD) and Iguaçu Region (IR). Analyses using nine microsatellites revealed mean observed and expected heterozygosity of 0.68 and 0.70, respectively. The MD sampled population showed evidence of a genetic bottleneck under two mutational models (TPM = 0.03711 and SMM = 0.04883). Estimates of genetic structure (FST = 0.027; best fit of k = 1 with STRUCTURE) revealed no meaningful differentiation between these populations. Thus, our results indicate that the ocelot populations sampled in these fragments are still not significantly different genetically, a pattern that strongly contrasts with that previously observed in jaguars for the same comparisons. This observation is likely due to a combination of two factors: (i) larger effective population size of ocelots (relative to jaguars) in each fragment, implying a slower effect of drift-induced differentiation; and (ii) potentially some remaining permeability of the anthropogenic matrix for ocelots, as opposed to the observed lack of permeability for jaguars. The persistence of ocelot gene flow between these areas must be prioritized in long-term conservation planning on behalf of these felids.Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Zootecnia, Via de Acesso Paulo Donato Castellane, s/nUniversidade Estadual do Sudoeste da Bahia Departamento de Ciências Biológicas, Av. José Moreira Sobrinho, s/nUniversidade de Brasília Instituto de Ciências Biológicas Departamento de Genética e Morfologia, Campus Universitário Darcy RibeiroPUCRS Faculdade de Biociências Laboratório de Biologia Genômica e Molecular, Av. Ipiranga 6681, Prédio 12Instituto Pró-CarnívorosUniversidade Federal de São João Del-Rei Departamento de Ciências Naturais Universidade Federal de São João del-Rei, Campus Dom BoscoInstituto de Pesquisas Ecológicas, Rua Ricardo Fogarolli, 387, Vila Sao PauloCentro Nacional de Pesquisa Para a Conservação de Predadores Naturais Instituto Chico Mendes de Conservação da Biodiversidade, Avenida dos Bandeirantes, s/n Balneário MunicipalLaboratório de Biodiversidade Molecular e Citogenética Universidade Federal de São Carlos Departamento de Genética e Evolução, Via Washington Luis, km 235, Caixa Postal 676Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Zootecnia, Via de Acesso Paulo Donato Castellane, s/nUniversidade Estadual Paulista (UNESP)Universidade Estadual do Sudoeste da BahiaUniversidade de Brasília (UnB)Laboratório de Biologia Genômica e MolecularInstituto Pró-CarnívorosUniversidade Federal de São João del-ReiInstituto de Pesquisas EcológicasInstituto Chico Mendes de Conservação da BiodiversidadeUniversidade Federal de São Carlos (UFSCar)Figueiredo, Marina G. [UNESP]Cervini, MarceloRodrigues, Fernando P.Eizirik, EduardoAzevedo, Fernando C.C.Cullen, LauryCrawshaw, Peter G.Galetti, Pedro M.2022-04-28T19:01:42Z2022-04-28T19:01:42Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article295-306http://dx.doi.org/10.3390/d7030295Diversity, v. 7, n. 3, p. 295-306, 2015.1424-2818http://hdl.handle.net/11449/22048410.3390/d70302952-s2.0-84946065507Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDiversityinfo:eu-repo/semantics/openAccess2022-04-28T19:01:42Zoai:repositorio.unesp.br:11449/220484Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:01:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Lack of Population Genetic Structuring in Ocelots (Leopardus pardalis) in a Fragmented Landscape |
title |
Lack of Population Genetic Structuring in Ocelots (Leopardus pardalis) in a Fragmented Landscape |
spellingShingle |
Lack of Population Genetic Structuring in Ocelots (Leopardus pardalis) in a Fragmented Landscape Figueiredo, Marina G. [UNESP] Bottleneck Felid Genetic diversity Habitat fragmentation |
title_short |
Lack of Population Genetic Structuring in Ocelots (Leopardus pardalis) in a Fragmented Landscape |
title_full |
Lack of Population Genetic Structuring in Ocelots (Leopardus pardalis) in a Fragmented Landscape |
title_fullStr |
Lack of Population Genetic Structuring in Ocelots (Leopardus pardalis) in a Fragmented Landscape |
title_full_unstemmed |
Lack of Population Genetic Structuring in Ocelots (Leopardus pardalis) in a Fragmented Landscape |
title_sort |
Lack of Population Genetic Structuring in Ocelots (Leopardus pardalis) in a Fragmented Landscape |
author |
Figueiredo, Marina G. [UNESP] |
author_facet |
Figueiredo, Marina G. [UNESP] Cervini, Marcelo Rodrigues, Fernando P. Eizirik, Eduardo Azevedo, Fernando C.C. Cullen, Laury Crawshaw, Peter G. Galetti, Pedro M. |
author_role |
author |
author2 |
Cervini, Marcelo Rodrigues, Fernando P. Eizirik, Eduardo Azevedo, Fernando C.C. Cullen, Laury Crawshaw, Peter G. Galetti, Pedro M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual do Sudoeste da Bahia Universidade de Brasília (UnB) Laboratório de Biologia Genômica e Molecular Instituto Pró-Carnívoros Universidade Federal de São João del-Rei Instituto de Pesquisas Ecológicas Instituto Chico Mendes de Conservação da Biodiversidade Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Figueiredo, Marina G. [UNESP] Cervini, Marcelo Rodrigues, Fernando P. Eizirik, Eduardo Azevedo, Fernando C.C. Cullen, Laury Crawshaw, Peter G. Galetti, Pedro M. |
dc.subject.por.fl_str_mv |
Bottleneck Felid Genetic diversity Habitat fragmentation |
topic |
Bottleneck Felid Genetic diversity Habitat fragmentation |
description |
Habitat fragmentation can promote patches of small and isolated populations, gene flow disruption between those populations, and reduction of local and total genetic variation. As a consequence, these small populations may go extinct in the long-term. The ocelot (Leopardus pardalis), originally distributed from Texas to southern Brazil and northern Argentina, has been impacted by habitat fragmentation throughout much of its range. To test whether habitat fragmentation has already induced genetic differentiation in an area where this process has been documented for a larger felid (jaguars), we analyzed molecular variation in ocelots inhabiting two Atlantic Forest fragments, Morro do Diabo (MD) and Iguaçu Region (IR). Analyses using nine microsatellites revealed mean observed and expected heterozygosity of 0.68 and 0.70, respectively. The MD sampled population showed evidence of a genetic bottleneck under two mutational models (TPM = 0.03711 and SMM = 0.04883). Estimates of genetic structure (FST = 0.027; best fit of k = 1 with STRUCTURE) revealed no meaningful differentiation between these populations. Thus, our results indicate that the ocelot populations sampled in these fragments are still not significantly different genetically, a pattern that strongly contrasts with that previously observed in jaguars for the same comparisons. This observation is likely due to a combination of two factors: (i) larger effective population size of ocelots (relative to jaguars) in each fragment, implying a slower effect of drift-induced differentiation; and (ii) potentially some remaining permeability of the anthropogenic matrix for ocelots, as opposed to the observed lack of permeability for jaguars. The persistence of ocelot gene flow between these areas must be prioritized in long-term conservation planning on behalf of these felids. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2022-04-28T19:01:42Z 2022-04-28T19:01:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/d7030295 Diversity, v. 7, n. 3, p. 295-306, 2015. 1424-2818 http://hdl.handle.net/11449/220484 10.3390/d7030295 2-s2.0-84946065507 |
url |
http://dx.doi.org/10.3390/d7030295 http://hdl.handle.net/11449/220484 |
identifier_str_mv |
Diversity, v. 7, n. 3, p. 295-306, 2015. 1424-2818 10.3390/d7030295 2-s2.0-84946065507 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diversity |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
295-306 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965689282297856 |