Timing carbon turnover (delta C-13) in weaned piglet's brain by IRMS
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0001-3765201820170861 http://hdl.handle.net/11449/164543 |
Resumo: | Isotope-ratio mass spectrometry (IRMS) is a potential tool that provides time-integrated estimate of assimilated and not just ingested nutrients. This feature turned possible its application to evaluate the effects of dietary nucleotides and glutamate on carbon turnover (delta C-13) in the brain of weanling piglets. Eighty-seven piglets weaned at 21-day-old were used, being three piglets slaughtered at day-0 (prior to diet-switch) and, the remaining 84 randomly assigned in a complete block design with a 2 x 2 factorial arrangement of treatments (two nucleotide levels: 0% and 0.1% and, two glutamate levels: 0% and 1%). On trial days 3, 6, 9, 14, 21, 35 and 49, three piglets per treatment were also slaughtered. The samples were analyzed by IRMS and adjusted to the first-order equation by a nonlinear regression analysis using NLIN procedure of SAS, in order to obtain the exponential graphics. The carbon turnover (t(95%)) verified for cerebral tissue was faster (P<0.05) for diet containing glutamate in comparison to other diets, supporting the fact that glutamate contributed to develop the piglets' brain, due to the fastest incorporation of C-13-atoms in this tissue at post-weaning phase, despite the energy deficit experienced by them. |
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Timing carbon turnover (delta C-13) in weaned piglet's brain by IRMSadditivesamino acidsisotopic dilutionstable isotopesswineIsotope-ratio mass spectrometry (IRMS) is a potential tool that provides time-integrated estimate of assimilated and not just ingested nutrients. This feature turned possible its application to evaluate the effects of dietary nucleotides and glutamate on carbon turnover (delta C-13) in the brain of weanling piglets. Eighty-seven piglets weaned at 21-day-old were used, being three piglets slaughtered at day-0 (prior to diet-switch) and, the remaining 84 randomly assigned in a complete block design with a 2 x 2 factorial arrangement of treatments (two nucleotide levels: 0% and 0.1% and, two glutamate levels: 0% and 1%). On trial days 3, 6, 9, 14, 21, 35 and 49, three piglets per treatment were also slaughtered. The samples were analyzed by IRMS and adjusted to the first-order equation by a nonlinear regression analysis using NLIN procedure of SAS, in order to obtain the exponential graphics. The carbon turnover (t(95%)) verified for cerebral tissue was faster (P<0.05) for diet containing glutamate in comparison to other diets, supporting the fact that glutamate contributed to develop the piglets' brain, due to the fastest incorporation of C-13-atoms in this tissue at post-weaning phase, despite the energy deficit experienced by them.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Prod Anim, Fac Med Vet & Zootecnia, Rua Dr Jose Barbosa Barros 1780, BR-18610307 Botucatu, SP, BrazilUniv Fed Mato Grosso, Inst Ciencias Agr & Tecnol, Ave Estudantes 5005, BR-78735902 Rondonopolis, MT, BrazilUniv Estadual Paulista, Inst Biociencias, Ctr Isotopos Estaveis Ambientais, Rua Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP, BrazilUniv Estadual Paulista, Dept Prod Anim, Fac Med Vet & Zootecnia, Rua Dr Jose Barbosa Barros 1780, BR-18610307 Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Ctr Isotopos Estaveis Ambientais, Rua Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP, BrazilFAPESP: 2014/13169-6FAPESP: 2013/25616-4Acad Brasileira De CienciasUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Saleh, Mayra A. D. [UNESP]Dos Santos, Luan S. [UNESP]Berto, Dirlei A. [UNESP]Amorim, Alessandro B.Costa, Vladimir E. [UNESP]Ducatti, Carlos [UNESP]2018-11-26T17:54:58Z2018-11-26T17:54:58Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2469-2478application/pdfhttp://dx.doi.org/10.1590/0001-3765201820170861Anais Da Academia Brasileira De Ciencias. Rio Janeiro: Acad Brasileira De Ciencias, v. 90, n. 2, p. 2469-2478, 2018.0001-3765http://hdl.handle.net/11449/16454310.1590/0001-3765201820170861S0001-37652018000502469WOS:000442096300045S0001-37652018000502469.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnais Da Academia Brasileira De Ciencias0,418info:eu-repo/semantics/openAccess2024-09-09T13:00:27Zoai:repositorio.unesp.br:11449/164543Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T13:00:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Timing carbon turnover (delta C-13) in weaned piglet's brain by IRMS |
title |
Timing carbon turnover (delta C-13) in weaned piglet's brain by IRMS |
spellingShingle |
Timing carbon turnover (delta C-13) in weaned piglet's brain by IRMS Saleh, Mayra A. D. [UNESP] additives amino acids isotopic dilution stable isotopes swine |
title_short |
Timing carbon turnover (delta C-13) in weaned piglet's brain by IRMS |
title_full |
Timing carbon turnover (delta C-13) in weaned piglet's brain by IRMS |
title_fullStr |
Timing carbon turnover (delta C-13) in weaned piglet's brain by IRMS |
title_full_unstemmed |
Timing carbon turnover (delta C-13) in weaned piglet's brain by IRMS |
title_sort |
Timing carbon turnover (delta C-13) in weaned piglet's brain by IRMS |
author |
Saleh, Mayra A. D. [UNESP] |
author_facet |
Saleh, Mayra A. D. [UNESP] Dos Santos, Luan S. [UNESP] Berto, Dirlei A. [UNESP] Amorim, Alessandro B. Costa, Vladimir E. [UNESP] Ducatti, Carlos [UNESP] |
author_role |
author |
author2 |
Dos Santos, Luan S. [UNESP] Berto, Dirlei A. [UNESP] Amorim, Alessandro B. Costa, Vladimir E. [UNESP] Ducatti, Carlos [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso do Sul (UFMS) |
dc.contributor.author.fl_str_mv |
Saleh, Mayra A. D. [UNESP] Dos Santos, Luan S. [UNESP] Berto, Dirlei A. [UNESP] Amorim, Alessandro B. Costa, Vladimir E. [UNESP] Ducatti, Carlos [UNESP] |
dc.subject.por.fl_str_mv |
additives amino acids isotopic dilution stable isotopes swine |
topic |
additives amino acids isotopic dilution stable isotopes swine |
description |
Isotope-ratio mass spectrometry (IRMS) is a potential tool that provides time-integrated estimate of assimilated and not just ingested nutrients. This feature turned possible its application to evaluate the effects of dietary nucleotides and glutamate on carbon turnover (delta C-13) in the brain of weanling piglets. Eighty-seven piglets weaned at 21-day-old were used, being three piglets slaughtered at day-0 (prior to diet-switch) and, the remaining 84 randomly assigned in a complete block design with a 2 x 2 factorial arrangement of treatments (two nucleotide levels: 0% and 0.1% and, two glutamate levels: 0% and 1%). On trial days 3, 6, 9, 14, 21, 35 and 49, three piglets per treatment were also slaughtered. The samples were analyzed by IRMS and adjusted to the first-order equation by a nonlinear regression analysis using NLIN procedure of SAS, in order to obtain the exponential graphics. The carbon turnover (t(95%)) verified for cerebral tissue was faster (P<0.05) for diet containing glutamate in comparison to other diets, supporting the fact that glutamate contributed to develop the piglets' brain, due to the fastest incorporation of C-13-atoms in this tissue at post-weaning phase, despite the energy deficit experienced by them. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:54:58Z 2018-11-26T17:54:58Z 2018-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0001-3765201820170861 Anais Da Academia Brasileira De Ciencias. Rio Janeiro: Acad Brasileira De Ciencias, v. 90, n. 2, p. 2469-2478, 2018. 0001-3765 http://hdl.handle.net/11449/164543 10.1590/0001-3765201820170861 S0001-37652018000502469 WOS:000442096300045 S0001-37652018000502469.pdf |
url |
http://dx.doi.org/10.1590/0001-3765201820170861 http://hdl.handle.net/11449/164543 |
identifier_str_mv |
Anais Da Academia Brasileira De Ciencias. Rio Janeiro: Acad Brasileira De Ciencias, v. 90, n. 2, p. 2469-2478, 2018. 0001-3765 10.1590/0001-3765201820170861 S0001-37652018000502469 WOS:000442096300045 S0001-37652018000502469.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anais Da Academia Brasileira De Ciencias 0,418 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2469-2478 application/pdf |
dc.publisher.none.fl_str_mv |
Acad Brasileira De Ciencias |
publisher.none.fl_str_mv |
Acad Brasileira De Ciencias |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021312570064896 |