Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers

Detalhes bibliográficos
Autor(a) principal: Wickert, Ester [UNESP]
Data de Publicação: 2007
Outros Autores: Machado, Marcos Antonio, Lemos, Eliana G. M. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1094/PHYTO-97-12-1543
http://hdl.handle.net/11449/35365
Resumo: The aim of this study was to obtain information about genetic diversity and make some inferences about the relationship of 27 strains of Xylella fastidiosa from different hosts and distinct geographical areas. Single-nucleotide polymorphism (SNP) molecular markers were identified in DNA sequences from 16 distinct regions of the genome of 24 strains of X. fastidiosa from coffee and citrus plants. Among the Brazilian strains, coffee-dependent strains have a greater number of SNPs (10 to 24 SNPs) than the citrus-based strains (2 to 12 SNPs); all the strains were compared with the sequenced strain 9a5c. The identified SNP markers were able to distinguish, for the first time, strains from citrus plants and coffee and showed that strains from coffee present higher genetic diversity than the others. These markers also have proven to be efficient for discriminating strains from the same host obtained from different geographic regions. X. fastidiosa, the causal agent of citrus variegated chlorosis, possesses genetic diversity, and the SNP markers were highly efficient for discriminating genetically close organisms.
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spelling Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markersThe aim of this study was to obtain information about genetic diversity and make some inferences about the relationship of 27 strains of Xylella fastidiosa from different hosts and distinct geographical areas. Single-nucleotide polymorphism (SNP) molecular markers were identified in DNA sequences from 16 distinct regions of the genome of 24 strains of X. fastidiosa from coffee and citrus plants. Among the Brazilian strains, coffee-dependent strains have a greater number of SNPs (10 to 24 SNPs) than the citrus-based strains (2 to 12 SNPs); all the strains were compared with the sequenced strain 9a5c. The identified SNP markers were able to distinguish, for the first time, strains from citrus plants and coffee and showed that strains from coffee present higher genetic diversity than the others. These markers also have proven to be efficient for discriminating strains from the same host obtained from different geographic regions. X. fastidiosa, the causal agent of citrus variegated chlorosis, possesses genetic diversity, and the SNP markers were highly efficient for discriminating genetically close organisms.UNESP, Sch Agr & Vet Sci, Microorganism & Plant Biochem Lab, Jaboticabal, SP, BrazilIAC, APTA Citrus, Biotechnol Lab, Cordeiropolis, SP, BrazilUNESP, Sch Agr & Vet Sci, Microorganism & Plant Biochem Lab, Jaboticabal, SP, BrazilAmer Phytopathological SocUniversidade Estadual Paulista (Unesp)IACWickert, Ester [UNESP]Machado, Marcos AntonioLemos, Eliana G. M. [UNESP]2014-05-20T15:24:50Z2014-05-20T15:24:50Z2007-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1543-1549http://dx.doi.org/10.1094/PHYTO-97-12-1543Phytopathology. St Paul: Amer Phytopathological Soc, v. 97, n. 12, p. 1543-1549, 2007.0031-949Xhttp://hdl.handle.net/11449/3536510.1094/PHYTO-97-12-1543WOS:000251599100002Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhytopathology3.036info:eu-repo/semantics/openAccess2024-06-07T15:32:35Zoai:repositorio.unesp.br:11449/35365Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:25:44.834669Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers
title Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers
spellingShingle Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers
Wickert, Ester [UNESP]
title_short Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers
title_full Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers
title_fullStr Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers
title_full_unstemmed Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers
title_sort Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers
author Wickert, Ester [UNESP]
author_facet Wickert, Ester [UNESP]
Machado, Marcos Antonio
Lemos, Eliana G. M. [UNESP]
author_role author
author2 Machado, Marcos Antonio
Lemos, Eliana G. M. [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
IAC
dc.contributor.author.fl_str_mv Wickert, Ester [UNESP]
Machado, Marcos Antonio
Lemos, Eliana G. M. [UNESP]
description The aim of this study was to obtain information about genetic diversity and make some inferences about the relationship of 27 strains of Xylella fastidiosa from different hosts and distinct geographical areas. Single-nucleotide polymorphism (SNP) molecular markers were identified in DNA sequences from 16 distinct regions of the genome of 24 strains of X. fastidiosa from coffee and citrus plants. Among the Brazilian strains, coffee-dependent strains have a greater number of SNPs (10 to 24 SNPs) than the citrus-based strains (2 to 12 SNPs); all the strains were compared with the sequenced strain 9a5c. The identified SNP markers were able to distinguish, for the first time, strains from citrus plants and coffee and showed that strains from coffee present higher genetic diversity than the others. These markers also have proven to be efficient for discriminating strains from the same host obtained from different geographic regions. X. fastidiosa, the causal agent of citrus variegated chlorosis, possesses genetic diversity, and the SNP markers were highly efficient for discriminating genetically close organisms.
publishDate 2007
dc.date.none.fl_str_mv 2007-12-01
2014-05-20T15:24:50Z
2014-05-20T15:24:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1094/PHYTO-97-12-1543
Phytopathology. St Paul: Amer Phytopathological Soc, v. 97, n. 12, p. 1543-1549, 2007.
0031-949X
http://hdl.handle.net/11449/35365
10.1094/PHYTO-97-12-1543
WOS:000251599100002
url http://dx.doi.org/10.1094/PHYTO-97-12-1543
http://hdl.handle.net/11449/35365
identifier_str_mv Phytopathology. St Paul: Amer Phytopathological Soc, v. 97, n. 12, p. 1543-1549, 2007.
0031-949X
10.1094/PHYTO-97-12-1543
WOS:000251599100002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Phytopathology
3.036
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1543-1549
dc.publisher.none.fl_str_mv Amer Phytopathological Soc
publisher.none.fl_str_mv Amer Phytopathological Soc
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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