Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1094/PHYTO-97-12-1543 http://hdl.handle.net/11449/35365 |
Resumo: | The aim of this study was to obtain information about genetic diversity and make some inferences about the relationship of 27 strains of Xylella fastidiosa from different hosts and distinct geographical areas. Single-nucleotide polymorphism (SNP) molecular markers were identified in DNA sequences from 16 distinct regions of the genome of 24 strains of X. fastidiosa from coffee and citrus plants. Among the Brazilian strains, coffee-dependent strains have a greater number of SNPs (10 to 24 SNPs) than the citrus-based strains (2 to 12 SNPs); all the strains were compared with the sequenced strain 9a5c. The identified SNP markers were able to distinguish, for the first time, strains from citrus plants and coffee and showed that strains from coffee present higher genetic diversity than the others. These markers also have proven to be efficient for discriminating strains from the same host obtained from different geographic regions. X. fastidiosa, the causal agent of citrus variegated chlorosis, possesses genetic diversity, and the SNP markers were highly efficient for discriminating genetically close organisms. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markersThe aim of this study was to obtain information about genetic diversity and make some inferences about the relationship of 27 strains of Xylella fastidiosa from different hosts and distinct geographical areas. Single-nucleotide polymorphism (SNP) molecular markers were identified in DNA sequences from 16 distinct regions of the genome of 24 strains of X. fastidiosa from coffee and citrus plants. Among the Brazilian strains, coffee-dependent strains have a greater number of SNPs (10 to 24 SNPs) than the citrus-based strains (2 to 12 SNPs); all the strains were compared with the sequenced strain 9a5c. The identified SNP markers were able to distinguish, for the first time, strains from citrus plants and coffee and showed that strains from coffee present higher genetic diversity than the others. These markers also have proven to be efficient for discriminating strains from the same host obtained from different geographic regions. X. fastidiosa, the causal agent of citrus variegated chlorosis, possesses genetic diversity, and the SNP markers were highly efficient for discriminating genetically close organisms.UNESP, Sch Agr & Vet Sci, Microorganism & Plant Biochem Lab, Jaboticabal, SP, BrazilIAC, APTA Citrus, Biotechnol Lab, Cordeiropolis, SP, BrazilUNESP, Sch Agr & Vet Sci, Microorganism & Plant Biochem Lab, Jaboticabal, SP, BrazilAmer Phytopathological SocUniversidade Estadual Paulista (Unesp)IACWickert, Ester [UNESP]Machado, Marcos AntonioLemos, Eliana G. M. [UNESP]2014-05-20T15:24:50Z2014-05-20T15:24:50Z2007-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1543-1549http://dx.doi.org/10.1094/PHYTO-97-12-1543Phytopathology. St Paul: Amer Phytopathological Soc, v. 97, n. 12, p. 1543-1549, 2007.0031-949Xhttp://hdl.handle.net/11449/3536510.1094/PHYTO-97-12-1543WOS:000251599100002Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhytopathology3.036info:eu-repo/semantics/openAccess2024-06-07T15:32:35Zoai:repositorio.unesp.br:11449/35365Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:25:44.834669Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers |
title |
Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers |
spellingShingle |
Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers Wickert, Ester [UNESP] |
title_short |
Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers |
title_full |
Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers |
title_fullStr |
Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers |
title_full_unstemmed |
Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers |
title_sort |
Evaluation of the genetic diversity of Xylella fastidiosa strains from citrus and coffee hosts by single-nucleotide polymorphism markers |
author |
Wickert, Ester [UNESP] |
author_facet |
Wickert, Ester [UNESP] Machado, Marcos Antonio Lemos, Eliana G. M. [UNESP] |
author_role |
author |
author2 |
Machado, Marcos Antonio Lemos, Eliana G. M. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) IAC |
dc.contributor.author.fl_str_mv |
Wickert, Ester [UNESP] Machado, Marcos Antonio Lemos, Eliana G. M. [UNESP] |
description |
The aim of this study was to obtain information about genetic diversity and make some inferences about the relationship of 27 strains of Xylella fastidiosa from different hosts and distinct geographical areas. Single-nucleotide polymorphism (SNP) molecular markers were identified in DNA sequences from 16 distinct regions of the genome of 24 strains of X. fastidiosa from coffee and citrus plants. Among the Brazilian strains, coffee-dependent strains have a greater number of SNPs (10 to 24 SNPs) than the citrus-based strains (2 to 12 SNPs); all the strains were compared with the sequenced strain 9a5c. The identified SNP markers were able to distinguish, for the first time, strains from citrus plants and coffee and showed that strains from coffee present higher genetic diversity than the others. These markers also have proven to be efficient for discriminating strains from the same host obtained from different geographic regions. X. fastidiosa, the causal agent of citrus variegated chlorosis, possesses genetic diversity, and the SNP markers were highly efficient for discriminating genetically close organisms. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-12-01 2014-05-20T15:24:50Z 2014-05-20T15:24:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1094/PHYTO-97-12-1543 Phytopathology. St Paul: Amer Phytopathological Soc, v. 97, n. 12, p. 1543-1549, 2007. 0031-949X http://hdl.handle.net/11449/35365 10.1094/PHYTO-97-12-1543 WOS:000251599100002 |
url |
http://dx.doi.org/10.1094/PHYTO-97-12-1543 http://hdl.handle.net/11449/35365 |
identifier_str_mv |
Phytopathology. St Paul: Amer Phytopathological Soc, v. 97, n. 12, p. 1543-1549, 2007. 0031-949X 10.1094/PHYTO-97-12-1543 WOS:000251599100002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Phytopathology 3.036 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1543-1549 |
dc.publisher.none.fl_str_mv |
Amer Phytopathological Soc |
publisher.none.fl_str_mv |
Amer Phytopathological Soc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129426946260992 |