Novaluron impairs the silk gland and productive performance of silkworm Bombyx mori (Lepidoptera: Bombycidae) larvae

Detalhes bibliográficos
Autor(a) principal: Santorum, Marilucia [UNESP]
Data de Publicação: 2020
Outros Autores: Costa, Rose Meire, dos Reis, Gustavo Henrique, Carvalho dos Santos, Daniela [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.chemosphere.2019.124697
http://hdl.handle.net/11449/199421
Resumo: This study investigates the effects of the insect growth regulator Novaluron on the silk gland (SG) and silk cocoon production in a nontarget insect, the silkworm Bombyx mori, which is a model research insect among Lepidoptera and of great economic importance for the commercial production of silk threads. Larvae were segregated into experimental groups: the control group (CG) and the treatment group (TG), which was exposed to a Novaluron concentration of 0.15 mL/L. Following exposure, we analyzed the cytotoxic effects on the epithelial cells of the anterior, middle and posterior regions of the SG of B. mori larvae in the 3rd, 4th, and 5th instars, as well as the quality of the cocoons from larvae in the 5th instar. Cytotoxic effects were observed in the TG, such as the dilation of cells, emission of cytoplasmic protrusions, extreme rarefaction of the cytoplasm and nuclei, dilation of the endoplasmic reticulum, intracellular and intercellular spaces, spacing between the epithelial cells and the basal lamina and detachment of some cells towards the lumen of the SG, and decreased protein in the lumen, with faults in its composition. In addition, we verified ultrastructural changes in the production of fibers and silk cocoons, including a reduction in the weight of the cocoons constructed by both males and females in the TG and the construction of defective cocoons. Novaluron exposure impairs the SG and may affect the physiological functions of this organ; additionally, it compromises the quality of silk cocoons, potentially causing serious damage to sericulture.
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spelling Novaluron impairs the silk gland and productive performance of silkworm Bombyx mori (Lepidoptera: Bombycidae) larvaeInsecticideMorphologySilk cocoonsToxicityUltrastructureThis study investigates the effects of the insect growth regulator Novaluron on the silk gland (SG) and silk cocoon production in a nontarget insect, the silkworm Bombyx mori, which is a model research insect among Lepidoptera and of great economic importance for the commercial production of silk threads. Larvae were segregated into experimental groups: the control group (CG) and the treatment group (TG), which was exposed to a Novaluron concentration of 0.15 mL/L. Following exposure, we analyzed the cytotoxic effects on the epithelial cells of the anterior, middle and posterior regions of the SG of B. mori larvae in the 3rd, 4th, and 5th instars, as well as the quality of the cocoons from larvae in the 5th instar. Cytotoxic effects were observed in the TG, such as the dilation of cells, emission of cytoplasmic protrusions, extreme rarefaction of the cytoplasm and nuclei, dilation of the endoplasmic reticulum, intracellular and intercellular spaces, spacing between the epithelial cells and the basal lamina and detachment of some cells towards the lumen of the SG, and decreased protein in the lumen, with faults in its composition. In addition, we verified ultrastructural changes in the production of fibers and silk cocoons, including a reduction in the weight of the cocoons constructed by both males and females in the TG and the construction of defective cocoons. Novaluron exposure impairs the SG and may affect the physiological functions of this organ; additionally, it compromises the quality of silk cocoons, potentially causing serious damage to sericulture.Laboratory of Insects Department of Morphology São Paulo State University (UNESP) Institute of BiosciencesCenter of Biological Sciences and Health State University of Western Paraná (UNIOESTE)Postgraduate in Pathology Federal University of Rio de Janeiro (UFRJ)Electron Microscopy Center of the Institute of Biosciences of Botucatu UNESPLaboratory of Insects Department of Morphology São Paulo State University (UNESP) Institute of BiosciencesElectron Microscopy Center of the Institute of Biosciences of Botucatu UNESPUniversidade Estadual Paulista (Unesp)State University of Western Paraná (UNIOESTE)Universidade Federal do Rio de Janeiro (UFRJ)Santorum, Marilucia [UNESP]Costa, Rose Meiredos Reis, Gustavo HenriqueCarvalho dos Santos, Daniela [UNESP]2020-12-12T01:39:18Z2020-12-12T01:39:18Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.chemosphere.2019.124697Chemosphere, v. 239.1879-12980045-6535http://hdl.handle.net/11449/19942110.1016/j.chemosphere.2019.1246972-s2.0-85071751283Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphereinfo:eu-repo/semantics/openAccess2024-04-11T14:57:11Zoai:repositorio.unesp.br:11449/199421Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:08:03.674986Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Novaluron impairs the silk gland and productive performance of silkworm Bombyx mori (Lepidoptera: Bombycidae) larvae
title Novaluron impairs the silk gland and productive performance of silkworm Bombyx mori (Lepidoptera: Bombycidae) larvae
spellingShingle Novaluron impairs the silk gland and productive performance of silkworm Bombyx mori (Lepidoptera: Bombycidae) larvae
Santorum, Marilucia [UNESP]
Insecticide
Morphology
Silk cocoons
Toxicity
Ultrastructure
title_short Novaluron impairs the silk gland and productive performance of silkworm Bombyx mori (Lepidoptera: Bombycidae) larvae
title_full Novaluron impairs the silk gland and productive performance of silkworm Bombyx mori (Lepidoptera: Bombycidae) larvae
title_fullStr Novaluron impairs the silk gland and productive performance of silkworm Bombyx mori (Lepidoptera: Bombycidae) larvae
title_full_unstemmed Novaluron impairs the silk gland and productive performance of silkworm Bombyx mori (Lepidoptera: Bombycidae) larvae
title_sort Novaluron impairs the silk gland and productive performance of silkworm Bombyx mori (Lepidoptera: Bombycidae) larvae
author Santorum, Marilucia [UNESP]
author_facet Santorum, Marilucia [UNESP]
Costa, Rose Meire
dos Reis, Gustavo Henrique
Carvalho dos Santos, Daniela [UNESP]
author_role author
author2 Costa, Rose Meire
dos Reis, Gustavo Henrique
Carvalho dos Santos, Daniela [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
State University of Western Paraná (UNIOESTE)
Universidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.author.fl_str_mv Santorum, Marilucia [UNESP]
Costa, Rose Meire
dos Reis, Gustavo Henrique
Carvalho dos Santos, Daniela [UNESP]
dc.subject.por.fl_str_mv Insecticide
Morphology
Silk cocoons
Toxicity
Ultrastructure
topic Insecticide
Morphology
Silk cocoons
Toxicity
Ultrastructure
description This study investigates the effects of the insect growth regulator Novaluron on the silk gland (SG) and silk cocoon production in a nontarget insect, the silkworm Bombyx mori, which is a model research insect among Lepidoptera and of great economic importance for the commercial production of silk threads. Larvae were segregated into experimental groups: the control group (CG) and the treatment group (TG), which was exposed to a Novaluron concentration of 0.15 mL/L. Following exposure, we analyzed the cytotoxic effects on the epithelial cells of the anterior, middle and posterior regions of the SG of B. mori larvae in the 3rd, 4th, and 5th instars, as well as the quality of the cocoons from larvae in the 5th instar. Cytotoxic effects were observed in the TG, such as the dilation of cells, emission of cytoplasmic protrusions, extreme rarefaction of the cytoplasm and nuclei, dilation of the endoplasmic reticulum, intracellular and intercellular spaces, spacing between the epithelial cells and the basal lamina and detachment of some cells towards the lumen of the SG, and decreased protein in the lumen, with faults in its composition. In addition, we verified ultrastructural changes in the production of fibers and silk cocoons, including a reduction in the weight of the cocoons constructed by both males and females in the TG and the construction of defective cocoons. Novaluron exposure impairs the SG and may affect the physiological functions of this organ; additionally, it compromises the quality of silk cocoons, potentially causing serious damage to sericulture.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:39:18Z
2020-12-12T01:39:18Z
2020-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.chemosphere.2019.124697
Chemosphere, v. 239.
1879-1298
0045-6535
http://hdl.handle.net/11449/199421
10.1016/j.chemosphere.2019.124697
2-s2.0-85071751283
url http://dx.doi.org/10.1016/j.chemosphere.2019.124697
http://hdl.handle.net/11449/199421
identifier_str_mv Chemosphere, v. 239.
1879-1298
0045-6535
10.1016/j.chemosphere.2019.124697
2-s2.0-85071751283
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chemosphere
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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