Bothrops alternatus Snake Venom Induces Cytokine Expression and Oxidative Stress on Renal Function

Detalhes bibliográficos
Autor(a) principal: Nogueira Junior, F. A.
Data de Publicação: 2019
Outros Autores: Coelho Jorge, A. R., Marinho, A. D., Moraes Silveira, J. A. de, Queiroz Alves, N. T., Sa Costa, P. H., Braga E Silva, P. L., Maia Chaves-Filho, A. J., Lima, D. B., Sampaio, T. L., Morais, G. B. de, Azul Monteiro Evangelista, J. S., Costa Martins, A. M., Ferreira Junior, R. S. [UNESP], Macedo, D. S., Bezerra Jorge, R. J., Azul Monteiro, H. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.2174/1568026619666190809100319
http://hdl.handle.net/11449/196309
Resumo: Background: Envenomation caused by Bothrops alternatus is common in Southern Brazil. Acute Kidney Injury occurs after Bothrops snakebite and more information is necessaryrequired to understand its mechanism. Objective: The objective was to evaluate the effect of Bothrops alternatus venom (BaV) on renal cells and rat isolated kidney function. Methods: Wistar rats (n = 6, weighing 260-320 g) were perfused with a Krebs-Henseleit solution containing 6 g 100 mL(-1) of bovine serum albumin. After 30 minutes, the kidneys were perfused with BaV to a final concentration of 1 and 3 mu gmL(-1); and subsequently were evaluated for Perfusion Pressure (PP), Renal Vascular Resistance (RVR), Urinary Flow (UF), Glomerular Filtration Rate (GFR), and percentage of electrolyte tubular transport. Renal histological analysis, cytokine release, oxidative stress and cytotoxicity in renal proximal tubular cells were assessed. Results: BaV reduced PP, RVR, GFR, UF, total and proximal sodium transport (% TNa+), and chloride (%TCl-) in the isolated kidney perfusion model. Histological analysis of perfused kidneys disclosed the presence of proteinaceous material in the glomeruli and renal tubules, vacuolar tubular epithelial cell degeneration, Bowman's capsule degeneration, swelling of glomerular epithelial cells, glomerular atrophy and degeneration, and the presence of intratubular protein. Cytokine release (TNF-alpha, IL-1 beta, IL-10) and oxidative stress were increased in the kidneys. The viability of LLC-MK2 cells (IC50: 221.3 mu g/mL) was decreased by BaV and necrosis was involved in cell death. Conclusion: These findings indicate that BaV modifies functional parameters in an isolated perfused kidney model and has cytotoxic effects on renal lineage cells.
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spelling Bothrops alternatus Snake Venom Induces Cytokine Expression and Oxidative Stress on Renal FunctionSnake envenomationBothrops alternatusKidney perfusionCytotoxicityNecrosisAcute kidney injuryBackground: Envenomation caused by Bothrops alternatus is common in Southern Brazil. Acute Kidney Injury occurs after Bothrops snakebite and more information is necessaryrequired to understand its mechanism. Objective: The objective was to evaluate the effect of Bothrops alternatus venom (BaV) on renal cells and rat isolated kidney function. Methods: Wistar rats (n = 6, weighing 260-320 g) were perfused with a Krebs-Henseleit solution containing 6 g 100 mL(-1) of bovine serum albumin. After 30 minutes, the kidneys were perfused with BaV to a final concentration of 1 and 3 mu gmL(-1); and subsequently were evaluated for Perfusion Pressure (PP), Renal Vascular Resistance (RVR), Urinary Flow (UF), Glomerular Filtration Rate (GFR), and percentage of electrolyte tubular transport. Renal histological analysis, cytokine release, oxidative stress and cytotoxicity in renal proximal tubular cells were assessed. Results: BaV reduced PP, RVR, GFR, UF, total and proximal sodium transport (% TNa+), and chloride (%TCl-) in the isolated kidney perfusion model. Histological analysis of perfused kidneys disclosed the presence of proteinaceous material in the glomeruli and renal tubules, vacuolar tubular epithelial cell degeneration, Bowman's capsule degeneration, swelling of glomerular epithelial cells, glomerular atrophy and degeneration, and the presence of intratubular protein. Cytokine release (TNF-alpha, IL-1 beta, IL-10) and oxidative stress were increased in the kidneys. The viability of LLC-MK2 cells (IC50: 221.3 mu g/mL) was decreased by BaV and necrosis was involved in cell death. Conclusion: These findings indicate that BaV modifies functional parameters in an isolated perfused kidney model and has cytotoxic effects on renal lineage cells.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Research Support Foundation of Ceara State (FUNCAP/CE)Univ Fed Ceara, Fac Med, Dept Physiol & Pharmacol, BR-60430270 Fortaleza, Ceara, BrazilUniv Fed Ceara, Dept Clin & Toxicol Anal, Fortaleza, Ceara, BrazilUniv Fed Ceara, Fac Med Vet, Fortaleza, Ceara, BrazilUniv Estadual Paulista, CEVAP, Botucatu, SP, BrazilUniv Estadual Paulista, CEVAP, Botucatu, SP, BrazilBentham Science Publ LtdUniv Fed CearaUniversidade Estadual Paulista (Unesp)Nogueira Junior, F. A.Coelho Jorge, A. R.Marinho, A. D.Moraes Silveira, J. A. deQueiroz Alves, N. T.Sa Costa, P. H.Braga E Silva, P. L.Maia Chaves-Filho, A. J.Lima, D. B.Sampaio, T. L.Morais, G. B. deAzul Monteiro Evangelista, J. S.Costa Martins, A. M.Ferreira Junior, R. S. [UNESP]Macedo, D. S.Bezerra Jorge, R. J.Azul Monteiro, H. S.2020-12-10T19:40:31Z2020-12-10T19:40:31Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2058-2068http://dx.doi.org/10.2174/1568026619666190809100319Current Topics In Medicinal Chemistry. Sharjah: Bentham Science Publ Ltd, v. 19, n. 22, p. 2058-2068, 2019.1568-0266http://hdl.handle.net/11449/19630910.2174/1568026619666190809100319WOS:000495117500010Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCurrent Topics In Medicinal Chemistryinfo:eu-repo/semantics/openAccess2024-04-11T15:28:26Zoai:repositorio.unesp.br:11449/196309Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:50:01.831110Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Bothrops alternatus Snake Venom Induces Cytokine Expression and Oxidative Stress on Renal Function
title Bothrops alternatus Snake Venom Induces Cytokine Expression and Oxidative Stress on Renal Function
spellingShingle Bothrops alternatus Snake Venom Induces Cytokine Expression and Oxidative Stress on Renal Function
Nogueira Junior, F. A.
Snake envenomation
Bothrops alternatus
Kidney perfusion
Cytotoxicity
Necrosis
Acute kidney injury
title_short Bothrops alternatus Snake Venom Induces Cytokine Expression and Oxidative Stress on Renal Function
title_full Bothrops alternatus Snake Venom Induces Cytokine Expression and Oxidative Stress on Renal Function
title_fullStr Bothrops alternatus Snake Venom Induces Cytokine Expression and Oxidative Stress on Renal Function
title_full_unstemmed Bothrops alternatus Snake Venom Induces Cytokine Expression and Oxidative Stress on Renal Function
title_sort Bothrops alternatus Snake Venom Induces Cytokine Expression and Oxidative Stress on Renal Function
author Nogueira Junior, F. A.
author_facet Nogueira Junior, F. A.
Coelho Jorge, A. R.
Marinho, A. D.
Moraes Silveira, J. A. de
Queiroz Alves, N. T.
Sa Costa, P. H.
Braga E Silva, P. L.
Maia Chaves-Filho, A. J.
Lima, D. B.
Sampaio, T. L.
Morais, G. B. de
Azul Monteiro Evangelista, J. S.
Costa Martins, A. M.
Ferreira Junior, R. S. [UNESP]
Macedo, D. S.
Bezerra Jorge, R. J.
Azul Monteiro, H. S.
author_role author
author2 Coelho Jorge, A. R.
Marinho, A. D.
Moraes Silveira, J. A. de
Queiroz Alves, N. T.
Sa Costa, P. H.
Braga E Silva, P. L.
Maia Chaves-Filho, A. J.
Lima, D. B.
Sampaio, T. L.
Morais, G. B. de
Azul Monteiro Evangelista, J. S.
Costa Martins, A. M.
Ferreira Junior, R. S. [UNESP]
Macedo, D. S.
Bezerra Jorge, R. J.
Azul Monteiro, H. S.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Fed Ceara
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Nogueira Junior, F. A.
Coelho Jorge, A. R.
Marinho, A. D.
Moraes Silveira, J. A. de
Queiroz Alves, N. T.
Sa Costa, P. H.
Braga E Silva, P. L.
Maia Chaves-Filho, A. J.
Lima, D. B.
Sampaio, T. L.
Morais, G. B. de
Azul Monteiro Evangelista, J. S.
Costa Martins, A. M.
Ferreira Junior, R. S. [UNESP]
Macedo, D. S.
Bezerra Jorge, R. J.
Azul Monteiro, H. S.
dc.subject.por.fl_str_mv Snake envenomation
Bothrops alternatus
Kidney perfusion
Cytotoxicity
Necrosis
Acute kidney injury
topic Snake envenomation
Bothrops alternatus
Kidney perfusion
Cytotoxicity
Necrosis
Acute kidney injury
description Background: Envenomation caused by Bothrops alternatus is common in Southern Brazil. Acute Kidney Injury occurs after Bothrops snakebite and more information is necessaryrequired to understand its mechanism. Objective: The objective was to evaluate the effect of Bothrops alternatus venom (BaV) on renal cells and rat isolated kidney function. Methods: Wistar rats (n = 6, weighing 260-320 g) were perfused with a Krebs-Henseleit solution containing 6 g 100 mL(-1) of bovine serum albumin. After 30 minutes, the kidneys were perfused with BaV to a final concentration of 1 and 3 mu gmL(-1); and subsequently were evaluated for Perfusion Pressure (PP), Renal Vascular Resistance (RVR), Urinary Flow (UF), Glomerular Filtration Rate (GFR), and percentage of electrolyte tubular transport. Renal histological analysis, cytokine release, oxidative stress and cytotoxicity in renal proximal tubular cells were assessed. Results: BaV reduced PP, RVR, GFR, UF, total and proximal sodium transport (% TNa+), and chloride (%TCl-) in the isolated kidney perfusion model. Histological analysis of perfused kidneys disclosed the presence of proteinaceous material in the glomeruli and renal tubules, vacuolar tubular epithelial cell degeneration, Bowman's capsule degeneration, swelling of glomerular epithelial cells, glomerular atrophy and degeneration, and the presence of intratubular protein. Cytokine release (TNF-alpha, IL-1 beta, IL-10) and oxidative stress were increased in the kidneys. The viability of LLC-MK2 cells (IC50: 221.3 mu g/mL) was decreased by BaV and necrosis was involved in cell death. Conclusion: These findings indicate that BaV modifies functional parameters in an isolated perfused kidney model and has cytotoxic effects on renal lineage cells.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
2020-12-10T19:40:31Z
2020-12-10T19:40:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.2174/1568026619666190809100319
Current Topics In Medicinal Chemistry. Sharjah: Bentham Science Publ Ltd, v. 19, n. 22, p. 2058-2068, 2019.
1568-0266
http://hdl.handle.net/11449/196309
10.2174/1568026619666190809100319
WOS:000495117500010
url http://dx.doi.org/10.2174/1568026619666190809100319
http://hdl.handle.net/11449/196309
identifier_str_mv Current Topics In Medicinal Chemistry. Sharjah: Bentham Science Publ Ltd, v. 19, n. 22, p. 2058-2068, 2019.
1568-0266
10.2174/1568026619666190809100319
WOS:000495117500010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Current Topics In Medicinal Chemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2058-2068
dc.publisher.none.fl_str_mv Bentham Science Publ Ltd
publisher.none.fl_str_mv Bentham Science Publ Ltd
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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