Role of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interaction
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000200181&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/128678 |
Resumo: | Chagas disease, caused by the intracellular protozoan Trypanosoma cruzi, is a serious health problem in Latin America. During this parasitic infection, the heart is one of the major organs affected. The pathogenesis of tissue remodelling, particularly regarding cardiomyocyte behaviour after parasite infection and the molecular mechanisms that occur immediately following parasite entry into host cells are not yet completely understood. When cells are infected with T. cruzi, they develop an inflammatory response, in which cyclooxygenase-2 (COX-2) catalyses rate-limiting steps in the arachidonic acid pathway. However, how the parasite interaction modulates COX-2 activity is poorly understood. In this study, the H9c2 cell line was used as our model and we investigated cellular and biochemical aspects during the initial 48 h of parasitic infection. Oscillatory activity of COX-2 was observed, which correlated with the control of the pro-inflammatory environment in infected cells. Interestingly, subcellular trafficking was also verified, correlated with the control of Cox-2 mRNA or the activated COX-2 protein in cells, which is directly connected with the assemble of stress granules structures. Our collective findings suggest that in the very early stage of the T. cruzi-host cell interaction, the parasite is able to modulate the cellular metabolism in order to survives. |
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Role of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interactionCardiac cellsChagas diseaseEnzyme activityFluorescence microscopyPro-inflammatory processSubcellular traffickingChagas disease, caused by the intracellular protozoan Trypanosoma cruzi, is a serious health problem in Latin America. During this parasitic infection, the heart is one of the major organs affected. The pathogenesis of tissue remodelling, particularly regarding cardiomyocyte behaviour after parasite infection and the molecular mechanisms that occur immediately following parasite entry into host cells are not yet completely understood. When cells are infected with T. cruzi, they develop an inflammatory response, in which cyclooxygenase-2 (COX-2) catalyses rate-limiting steps in the arachidonic acid pathway. However, how the parasite interaction modulates COX-2 activity is poorly understood. In this study, the H9c2 cell line was used as our model and we investigated cellular and biochemical aspects during the initial 48 h of parasitic infection. Oscillatory activity of COX-2 was observed, which correlated with the control of the pro-inflammatory environment in infected cells. Interestingly, subcellular trafficking was also verified, correlated with the control of Cox-2 mRNA or the activated COX-2 protein in cells, which is directly connected with the assemble of stress granules structures. Our collective findings suggest that in the very early stage of the T. cruzi-host cell interaction, the parasite is able to modulate the cellular metabolism in order to survives.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)PROPE-UFOPUniv Estadual Paulista, Inst Biociencias, Dept Biol, Lab Biol Mol, Rio Claro, SP, BrazilUniv Fed Ouro Preto, Dept Ciencias Biol, Lab Doenca Chagas, Nucleo Pesquisa Ciencias Biol, Ouro Preto, MG, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Biol, Lab Biol Mol, Rio Claro, SP, BrazilFAPEMIG: CBB-APQ-02351-10FAPEMIG: CBB-APQ-01700-11CNPq: 475586/2009-3Fundaco Oswaldo CruzUniversidade Estadual Paulista (Unesp)Universidade Federal de Ouro Preto (UFOP)Moraes, Karen C. M. [UNESP]Diniz, Livia F.Bahia, Maria Terezinha2015-10-21T13:12:12Z2015-10-21T13:12:12Z2015-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article181-191application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000200181&lng=en&nrm=iso&tlng=enMemorias do Instituto Oswaldo Cruz, v. 110, n. 2, p. 181-191, 2015.0074-0276http://hdl.handle.net/11449/12867810.1590/0074-02760140311S0074-02762015000200181WOS:000354066400004S0074-02762015000200181.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemorias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2023-10-19T06:07:39Zoai:repositorio.unesp.br:11449/128678Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:21:03.313599Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Role of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interaction |
title |
Role of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interaction |
spellingShingle |
Role of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interaction Moraes, Karen C. M. [UNESP] Cardiac cells Chagas disease Enzyme activity Fluorescence microscopy Pro-inflammatory process Subcellular trafficking |
title_short |
Role of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interaction |
title_full |
Role of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interaction |
title_fullStr |
Role of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interaction |
title_full_unstemmed |
Role of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interaction |
title_sort |
Role of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interaction |
author |
Moraes, Karen C. M. [UNESP] |
author_facet |
Moraes, Karen C. M. [UNESP] Diniz, Livia F. Bahia, Maria Terezinha |
author_role |
author |
author2 |
Diniz, Livia F. Bahia, Maria Terezinha |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Ouro Preto (UFOP) |
dc.contributor.author.fl_str_mv |
Moraes, Karen C. M. [UNESP] Diniz, Livia F. Bahia, Maria Terezinha |
dc.subject.por.fl_str_mv |
Cardiac cells Chagas disease Enzyme activity Fluorescence microscopy Pro-inflammatory process Subcellular trafficking |
topic |
Cardiac cells Chagas disease Enzyme activity Fluorescence microscopy Pro-inflammatory process Subcellular trafficking |
description |
Chagas disease, caused by the intracellular protozoan Trypanosoma cruzi, is a serious health problem in Latin America. During this parasitic infection, the heart is one of the major organs affected. The pathogenesis of tissue remodelling, particularly regarding cardiomyocyte behaviour after parasite infection and the molecular mechanisms that occur immediately following parasite entry into host cells are not yet completely understood. When cells are infected with T. cruzi, they develop an inflammatory response, in which cyclooxygenase-2 (COX-2) catalyses rate-limiting steps in the arachidonic acid pathway. However, how the parasite interaction modulates COX-2 activity is poorly understood. In this study, the H9c2 cell line was used as our model and we investigated cellular and biochemical aspects during the initial 48 h of parasitic infection. Oscillatory activity of COX-2 was observed, which correlated with the control of the pro-inflammatory environment in infected cells. Interestingly, subcellular trafficking was also verified, correlated with the control of Cox-2 mRNA or the activated COX-2 protein in cells, which is directly connected with the assemble of stress granules structures. Our collective findings suggest that in the very early stage of the T. cruzi-host cell interaction, the parasite is able to modulate the cellular metabolism in order to survives. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T13:12:12Z 2015-10-21T13:12:12Z 2015-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000200181&lng=en&nrm=iso&tlng=en Memorias do Instituto Oswaldo Cruz, v. 110, n. 2, p. 181-191, 2015. 0074-0276 http://hdl.handle.net/11449/128678 10.1590/0074-02760140311 S0074-02762015000200181 WOS:000354066400004 S0074-02762015000200181.pdf |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000200181&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/128678 |
identifier_str_mv |
Memorias do Instituto Oswaldo Cruz, v. 110, n. 2, p. 181-191, 2015. 0074-0276 10.1590/0074-02760140311 S0074-02762015000200181 WOS:000354066400004 S0074-02762015000200181.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memorias do Instituto Oswaldo Cruz 2.833 1,172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
181-191 application/pdf |
dc.publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128501632466944 |