Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1677-3225v13n2a01 http://hdl.handle.net/11449/114631 |
Resumo: | AIM: To assess the combined use of tetracycline (TTC) and ethylenediaminetetraacetic acid (EDTA) on clot formation, considering that EDTA may neutralize TTC acidity. METHODS: Planed human tooth roots were treated with saline solution, EDTA, TTC and their combination (EDTA followed by TTC and TTC before EDTA). Fresh human blood was applied on the conditioned surfaces to check clot adhesion and stabilization. A previously calibrated (kappa = 0.93) and blinded examiner scored scanning electron micrographs of the samples. Statistical analyses were performed using one-way ANOVA and Tukey's test. RESULTS: Application of TTC before EDTA presented the best results with the highest number of cells adhered to the root surface (p=0.046). Use of EDTA alone and EDTA before TTC disturbed clot stabilization when compared to control group (p<0.01). CONCLUSIONS: The use of TTC before EDTA seems to be able to keep blood cells viable to establish an organized clot and could be used by clinicians together with the conventional mechanical root scaling and planing. |
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Repositório Institucional da UNESP |
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2946 |
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Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTAethylenediaminetetraacetic acidperiodontal regenerationscanning electron microscopysmear layerAIM: To assess the combined use of tetracycline (TTC) and ethylenediaminetetraacetic acid (EDTA) on clot formation, considering that EDTA may neutralize TTC acidity. METHODS: Planed human tooth roots were treated with saline solution, EDTA, TTC and their combination (EDTA followed by TTC and TTC before EDTA). Fresh human blood was applied on the conditioned surfaces to check clot adhesion and stabilization. A previously calibrated (kappa = 0.93) and blinded examiner scored scanning electron micrographs of the samples. Statistical analyses were performed using one-way ANOVA and Tukey's test. RESULTS: Application of TTC before EDTA presented the best results with the highest number of cells adhered to the root surface (p=0.046). Use of EDTA alone and EDTA before TTC disturbed clot stabilization when compared to control group (p<0.01). CONCLUSIONS: The use of TTC before EDTA seems to be able to keep blood cells viable to establish an organized clot and could be used by clinicians together with the conventional mechanical root scaling and planing.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de Pelotas School of Dentistry Department of Semiology and ClinicsUniversidade Estadual Paulista School of Pharmaceutical Sciences Department of Clinical AnalysisUniversidade Estadual Paulista Araraquara Dental School Department of Diagnosis and SurgeryUniversidade Estadual Paulista School of Pharmaceutical Sciences Department of Clinical AnalysisUniversidade Estadual Paulista Araraquara Dental School Department of Diagnosis and SurgeryFaculdade de Odontologia de Piracicaba - UNICAMPUniversidade Federal de Pelotas (UFPEL)Universidade Estadual Paulista (Unesp)Nascimento, Gustavo GiacomelliLeite, Amauri AntiqueraLeite, Elza Regina ManzolliSampaio, José Eduardo Cezar [UNESP]Leite, Fábio Renato Manzolli2015-02-02T12:39:43Z2015-02-02T12:39:43Z2014-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article83-88application/pdfhttp://dx.doi.org/10.1590/1677-3225v13n2a01Brazilian Journal of Oral Sciences. Faculdade de Odontologia de Piracicaba - UNICAMP, v. 13, n. 2, p. 83-88, 2014.1677-3225http://hdl.handle.net/11449/11463110.1590/1677-3225v13n2a01S1677-32252014000200083S1677-32252014000200083.pdf9531839078426485SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Oral Sciences0,131info:eu-repo/semantics/openAccess2023-10-04T06:02:05Zoai:repositorio.unesp.br:11449/114631Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-04T06:02:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA |
title |
Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA |
spellingShingle |
Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA Nascimento, Gustavo Giacomelli ethylenediaminetetraacetic acid periodontal regeneration scanning electron microscopy smear layer |
title_short |
Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA |
title_full |
Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA |
title_fullStr |
Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA |
title_full_unstemmed |
Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA |
title_sort |
Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA |
author |
Nascimento, Gustavo Giacomelli |
author_facet |
Nascimento, Gustavo Giacomelli Leite, Amauri Antiquera Leite, Elza Regina Manzolli Sampaio, José Eduardo Cezar [UNESP] Leite, Fábio Renato Manzolli |
author_role |
author |
author2 |
Leite, Amauri Antiquera Leite, Elza Regina Manzolli Sampaio, José Eduardo Cezar [UNESP] Leite, Fábio Renato Manzolli |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Pelotas (UFPEL) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Nascimento, Gustavo Giacomelli Leite, Amauri Antiquera Leite, Elza Regina Manzolli Sampaio, José Eduardo Cezar [UNESP] Leite, Fábio Renato Manzolli |
dc.subject.por.fl_str_mv |
ethylenediaminetetraacetic acid periodontal regeneration scanning electron microscopy smear layer |
topic |
ethylenediaminetetraacetic acid periodontal regeneration scanning electron microscopy smear layer |
description |
AIM: To assess the combined use of tetracycline (TTC) and ethylenediaminetetraacetic acid (EDTA) on clot formation, considering that EDTA may neutralize TTC acidity. METHODS: Planed human tooth roots were treated with saline solution, EDTA, TTC and their combination (EDTA followed by TTC and TTC before EDTA). Fresh human blood was applied on the conditioned surfaces to check clot adhesion and stabilization. A previously calibrated (kappa = 0.93) and blinded examiner scored scanning electron micrographs of the samples. Statistical analyses were performed using one-way ANOVA and Tukey's test. RESULTS: Application of TTC before EDTA presented the best results with the highest number of cells adhered to the root surface (p=0.046). Use of EDTA alone and EDTA before TTC disturbed clot stabilization when compared to control group (p<0.01). CONCLUSIONS: The use of TTC before EDTA seems to be able to keep blood cells viable to establish an organized clot and could be used by clinicians together with the conventional mechanical root scaling and planing. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01 2015-02-02T12:39:43Z 2015-02-02T12:39:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1677-3225v13n2a01 Brazilian Journal of Oral Sciences. Faculdade de Odontologia de Piracicaba - UNICAMP, v. 13, n. 2, p. 83-88, 2014. 1677-3225 http://hdl.handle.net/11449/114631 10.1590/1677-3225v13n2a01 S1677-32252014000200083 S1677-32252014000200083.pdf 9531839078426485 |
url |
http://dx.doi.org/10.1590/1677-3225v13n2a01 http://hdl.handle.net/11449/114631 |
identifier_str_mv |
Brazilian Journal of Oral Sciences. Faculdade de Odontologia de Piracicaba - UNICAMP, v. 13, n. 2, p. 83-88, 2014. 1677-3225 10.1590/1677-3225v13n2a01 S1677-32252014000200083 S1677-32252014000200083.pdf 9531839078426485 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Oral Sciences 0,131 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
83-88 application/pdf |
dc.publisher.none.fl_str_mv |
Faculdade de Odontologia de Piracicaba - UNICAMP |
publisher.none.fl_str_mv |
Faculdade de Odontologia de Piracicaba - UNICAMP |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964424925085696 |