Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3897/CompCytogen.v6i2.1769 http://hdl.handle.net/11449/18767 |
Resumo: | The aim of this study was to characterize cytogenetically one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907), with emphasis on the analysis of B chromosomes. The nucleolar activity in the B microchromosomes was characterized, and an analysis of mitotic instability of these microchromosomes was accomplished. The results showed a diploid chromosome number of 50 chromosomes. In all individuals, we observed the presence of B microchromosomes with intra- and inter-individual variability. The analysis of the nucleolus organizing regions (NORs) by silver nitrate staining demonstrated multiple NORs. We observed active sites of ribosomal DNA in the B microchromosomes, with a frequency of 20% in the analyzed cells, which shows gene activity in these chromosomal elements. The analysis of constitutive heterochromatin patterns showed that the B microchromosomes are heterochromatic or euchromatic, which demonstrates differentiation of DNA composition between these genomic elements. The calculation of the mitotic instability index implied that B chromosomes in this species might be in a final stage of instability. |
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Repositório Institucional da UNESP |
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Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes)fish cytogeneticNOR expressionsupernumerary chromosomesmitotic instabilityThe aim of this study was to characterize cytogenetically one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907), with emphasis on the analysis of B chromosomes. The nucleolar activity in the B microchromosomes was characterized, and an analysis of mitotic instability of these microchromosomes was accomplished. The results showed a diploid chromosome number of 50 chromosomes. In all individuals, we observed the presence of B microchromosomes with intra- and inter-individual variability. The analysis of the nucleolus organizing regions (NORs) by silver nitrate staining demonstrated multiple NORs. We observed active sites of ribosomal DNA in the B microchromosomes, with a frequency of 20% in the analyzed cells, which shows gene activity in these chromosomal elements. The analysis of constitutive heterochromatin patterns showed that the B microchromosomes are heterochromatic or euchromatic, which demonstrates differentiation of DNA composition between these genomic elements. The calculation of the mitotic instability index implied that B chromosomes in this species might be in a final stage of instability.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista UNESP, Fac Ciencias, Dept Ciencias Biol, BR-17033360 Bauru, SP, BrazilUniv Estadual Paulista UNESP, Inst Biociencias, Dept Morfol, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista UNESP, Fac Ciencias, Dept Ciencias Biol, BR-17033360 Bauru, SP, BrazilUniv Estadual Paulista UNESP, Inst Biociencias, Dept Morfol, BR-18618000 Botucatu, SP, BrazilZoological InstUniversidade Estadual Paulista (Unesp)Hashimoto, Diogo Teruo [UNESP]Voltolin, Tatiana Aparecida [UNESP]Valim de Arruda Paes, Ana Danyelle Noitel [UNESP]Foresti, Fausto [UNESP]Bortolozzi, Jehud [UNESP]Porto-Foresti, Fábio [UNESP]2014-05-20T13:52:35Z2014-05-20T13:52:35Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article141-151application/pdfhttp://dx.doi.org/10.3897/CompCytogen.v6i2.1769Comparative Cytogenetics. St Petersburg: Zoological Inst, v. 6, n. 2, p. 141-151, 2012.1993-0771http://hdl.handle.net/11449/1876710.3897/CompCytogen.v6i2.1769WOS:000307194600004WOS000307194600004.pdf3815338174165832Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Cytogenetics1.3190,431info:eu-repo/semantics/openAccess2024-04-23T15:23:49Zoai:repositorio.unesp.br:11449/18767Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:51:30.873834Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes) |
title |
Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes) |
spellingShingle |
Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes) Hashimoto, Diogo Teruo [UNESP] fish cytogenetic NOR expression supernumerary chromosomes mitotic instability |
title_short |
Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes) |
title_full |
Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes) |
title_fullStr |
Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes) |
title_full_unstemmed |
Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes) |
title_sort |
Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes) |
author |
Hashimoto, Diogo Teruo [UNESP] |
author_facet |
Hashimoto, Diogo Teruo [UNESP] Voltolin, Tatiana Aparecida [UNESP] Valim de Arruda Paes, Ana Danyelle Noitel [UNESP] Foresti, Fausto [UNESP] Bortolozzi, Jehud [UNESP] Porto-Foresti, Fábio [UNESP] |
author_role |
author |
author2 |
Voltolin, Tatiana Aparecida [UNESP] Valim de Arruda Paes, Ana Danyelle Noitel [UNESP] Foresti, Fausto [UNESP] Bortolozzi, Jehud [UNESP] Porto-Foresti, Fábio [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Hashimoto, Diogo Teruo [UNESP] Voltolin, Tatiana Aparecida [UNESP] Valim de Arruda Paes, Ana Danyelle Noitel [UNESP] Foresti, Fausto [UNESP] Bortolozzi, Jehud [UNESP] Porto-Foresti, Fábio [UNESP] |
dc.subject.por.fl_str_mv |
fish cytogenetic NOR expression supernumerary chromosomes mitotic instability |
topic |
fish cytogenetic NOR expression supernumerary chromosomes mitotic instability |
description |
The aim of this study was to characterize cytogenetically one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907), with emphasis on the analysis of B chromosomes. The nucleolar activity in the B microchromosomes was characterized, and an analysis of mitotic instability of these microchromosomes was accomplished. The results showed a diploid chromosome number of 50 chromosomes. In all individuals, we observed the presence of B microchromosomes with intra- and inter-individual variability. The analysis of the nucleolus organizing regions (NORs) by silver nitrate staining demonstrated multiple NORs. We observed active sites of ribosomal DNA in the B microchromosomes, with a frequency of 20% in the analyzed cells, which shows gene activity in these chromosomal elements. The analysis of constitutive heterochromatin patterns showed that the B microchromosomes are heterochromatic or euchromatic, which demonstrates differentiation of DNA composition between these genomic elements. The calculation of the mitotic instability index implied that B chromosomes in this species might be in a final stage of instability. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2014-05-20T13:52:35Z 2014-05-20T13:52:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3897/CompCytogen.v6i2.1769 Comparative Cytogenetics. St Petersburg: Zoological Inst, v. 6, n. 2, p. 141-151, 2012. 1993-0771 http://hdl.handle.net/11449/18767 10.3897/CompCytogen.v6i2.1769 WOS:000307194600004 WOS000307194600004.pdf 3815338174165832 |
url |
http://dx.doi.org/10.3897/CompCytogen.v6i2.1769 http://hdl.handle.net/11449/18767 |
identifier_str_mv |
Comparative Cytogenetics. St Petersburg: Zoological Inst, v. 6, n. 2, p. 141-151, 2012. 1993-0771 10.3897/CompCytogen.v6i2.1769 WOS:000307194600004 WOS000307194600004.pdf 3815338174165832 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Cytogenetics 1.319 0,431 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
141-151 application/pdf |
dc.publisher.none.fl_str_mv |
Zoological Inst |
publisher.none.fl_str_mv |
Zoological Inst |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129259084972032 |