Transcriptomic profile of longissimus thoracis associated with fatty acid content in Nellore beef cattle
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/age.13199 http://hdl.handle.net/11449/234343 |
Resumo: | The beef fatty acid (FA) profile has the potential to impact human health, and displays polygenic and complex features. This study aimed to identify the transcriptomic FA profile in the longissimus thoracis muscle in Nellore beef cattle finished in feedlot. Forty-four young bulls were sampled to assess the beef FA profile by considering 14 phenotypes and including differentially expressed genes (DEG), co-expressed (COE), and differentially co-expressed genes (DCO) analyses. All samples (n = 44) were used for COE analysis, whereas 30 samples with extreme phenotypes for the beef FA profile were used for DEG and DCO. A total of 912 DEG were identified, and the polyunsaturated (n = 563) and unsaturated ω-3 (n = 346) FA sums groups were the most frequently observed. The COE analyses identified three modules, of which the blue module (n = 1776) was correlated with eight of 14 FA phenotypes. Also, 759 DCO genes were listed, and the oleic acid (n = 358) and monounsaturated fatty acids sum (n = 120) were the most frequent. Furthermore, 243 and 13, 319 and seven, and 173 and 12 gene ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways were enriched respectively for the DEG, COE, and DCO analyses. Combining the results, we highlight the unexplored GIPC2, ASB5, and PPP5C genes in cattle. Besides LIPE and INSIG2 genes in COE modules, the ACSL3, ECI1, DECR2, FITM1, and SDHB genes were signaled in at least two analyses. These findings contribute to understand the genetic mechanisms underlying the beef FA profile in Nellore beef cattle finished in feedlot. |
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Transcriptomic profile of longissimus thoracis associated with fatty acid content in Nellore beef cattleBos taurus indicusco-expressionDEGfatty acid profilemeat qualityRNA-seqThe beef fatty acid (FA) profile has the potential to impact human health, and displays polygenic and complex features. This study aimed to identify the transcriptomic FA profile in the longissimus thoracis muscle in Nellore beef cattle finished in feedlot. Forty-four young bulls were sampled to assess the beef FA profile by considering 14 phenotypes and including differentially expressed genes (DEG), co-expressed (COE), and differentially co-expressed genes (DCO) analyses. All samples (n = 44) were used for COE analysis, whereas 30 samples with extreme phenotypes for the beef FA profile were used for DEG and DCO. A total of 912 DEG were identified, and the polyunsaturated (n = 563) and unsaturated ω-3 (n = 346) FA sums groups were the most frequently observed. The COE analyses identified three modules, of which the blue module (n = 1776) was correlated with eight of 14 FA phenotypes. Also, 759 DCO genes were listed, and the oleic acid (n = 358) and monounsaturated fatty acids sum (n = 120) were the most frequent. Furthermore, 243 and 13, 319 and seven, and 173 and 12 gene ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways were enriched respectively for the DEG, COE, and DCO analyses. Combining the results, we highlight the unexplored GIPC2, ASB5, and PPP5C genes in cattle. Besides LIPE and INSIG2 genes in COE modules, the ACSL3, ECI1, DECR2, FITM1, and SDHB genes were signaled in at least two analyses. These findings contribute to understand the genetic mechanisms underlying the beef FA profile in Nellore beef cattle finished in feedlot.School of Agricultural and Veterinary Sciences (FCAV) São Paulo State University (UNESP)School of Veterinary Medicine and Animal Science (FMVZ) University of São Paulo (USP)Commonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture & FoodSchool of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP)School of Agricultural and Veterinary Sciences (FCAV) São Paulo State University (UNESP)School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Agriculture & FoodSchettini, Gustavo Pimenta [UNESP]Peripolli, ElisaAlexandre, Pâmela Almeidados Santos, Wellington Bizarria [UNESP]da Silva Neto, João Barbosa [UNESP]Pereira, Angélica Simone Cravode Albuquerque, Lúcia Galvão [UNESP]Curi, Rogério Abdallah [UNESP]Baldi, Fernando [UNESP]2022-05-01T16:02:26Z2022-05-01T16:02:26Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/age.13199Animal Genetics.1365-20520268-9146http://hdl.handle.net/11449/23434310.1111/age.131992-s2.0-85127458394Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Geneticsinfo:eu-repo/semantics/openAccess2024-06-07T18:43:48Zoai:repositorio.unesp.br:11449/234343Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-07T18:43:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transcriptomic profile of longissimus thoracis associated with fatty acid content in Nellore beef cattle |
title |
Transcriptomic profile of longissimus thoracis associated with fatty acid content in Nellore beef cattle |
spellingShingle |
Transcriptomic profile of longissimus thoracis associated with fatty acid content in Nellore beef cattle Schettini, Gustavo Pimenta [UNESP] Bos taurus indicus co-expression DEG fatty acid profile meat quality RNA-seq |
title_short |
Transcriptomic profile of longissimus thoracis associated with fatty acid content in Nellore beef cattle |
title_full |
Transcriptomic profile of longissimus thoracis associated with fatty acid content in Nellore beef cattle |
title_fullStr |
Transcriptomic profile of longissimus thoracis associated with fatty acid content in Nellore beef cattle |
title_full_unstemmed |
Transcriptomic profile of longissimus thoracis associated with fatty acid content in Nellore beef cattle |
title_sort |
Transcriptomic profile of longissimus thoracis associated with fatty acid content in Nellore beef cattle |
author |
Schettini, Gustavo Pimenta [UNESP] |
author_facet |
Schettini, Gustavo Pimenta [UNESP] Peripolli, Elisa Alexandre, Pâmela Almeida dos Santos, Wellington Bizarria [UNESP] da Silva Neto, João Barbosa [UNESP] Pereira, Angélica Simone Cravo de Albuquerque, Lúcia Galvão [UNESP] Curi, Rogério Abdallah [UNESP] Baldi, Fernando [UNESP] |
author_role |
author |
author2 |
Peripolli, Elisa Alexandre, Pâmela Almeida dos Santos, Wellington Bizarria [UNESP] da Silva Neto, João Barbosa [UNESP] Pereira, Angélica Simone Cravo de Albuquerque, Lúcia Galvão [UNESP] Curi, Rogério Abdallah [UNESP] Baldi, Fernando [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Agriculture & Food |
dc.contributor.author.fl_str_mv |
Schettini, Gustavo Pimenta [UNESP] Peripolli, Elisa Alexandre, Pâmela Almeida dos Santos, Wellington Bizarria [UNESP] da Silva Neto, João Barbosa [UNESP] Pereira, Angélica Simone Cravo de Albuquerque, Lúcia Galvão [UNESP] Curi, Rogério Abdallah [UNESP] Baldi, Fernando [UNESP] |
dc.subject.por.fl_str_mv |
Bos taurus indicus co-expression DEG fatty acid profile meat quality RNA-seq |
topic |
Bos taurus indicus co-expression DEG fatty acid profile meat quality RNA-seq |
description |
The beef fatty acid (FA) profile has the potential to impact human health, and displays polygenic and complex features. This study aimed to identify the transcriptomic FA profile in the longissimus thoracis muscle in Nellore beef cattle finished in feedlot. Forty-four young bulls were sampled to assess the beef FA profile by considering 14 phenotypes and including differentially expressed genes (DEG), co-expressed (COE), and differentially co-expressed genes (DCO) analyses. All samples (n = 44) were used for COE analysis, whereas 30 samples with extreme phenotypes for the beef FA profile were used for DEG and DCO. A total of 912 DEG were identified, and the polyunsaturated (n = 563) and unsaturated ω-3 (n = 346) FA sums groups were the most frequently observed. The COE analyses identified three modules, of which the blue module (n = 1776) was correlated with eight of 14 FA phenotypes. Also, 759 DCO genes were listed, and the oleic acid (n = 358) and monounsaturated fatty acids sum (n = 120) were the most frequent. Furthermore, 243 and 13, 319 and seven, and 173 and 12 gene ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways were enriched respectively for the DEG, COE, and DCO analyses. Combining the results, we highlight the unexplored GIPC2, ASB5, and PPP5C genes in cattle. Besides LIPE and INSIG2 genes in COE modules, the ACSL3, ECI1, DECR2, FITM1, and SDHB genes were signaled in at least two analyses. These findings contribute to understand the genetic mechanisms underlying the beef FA profile in Nellore beef cattle finished in feedlot. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01T16:02:26Z 2022-05-01T16:02:26Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/age.13199 Animal Genetics. 1365-2052 0268-9146 http://hdl.handle.net/11449/234343 10.1111/age.13199 2-s2.0-85127458394 |
url |
http://dx.doi.org/10.1111/age.13199 http://hdl.handle.net/11449/234343 |
identifier_str_mv |
Animal Genetics. 1365-2052 0268-9146 10.1111/age.13199 2-s2.0-85127458394 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Genetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045483649695744 |