Assessment of genetic relationship between klebsiella pneumoniae and klebsiella oxytoca samples isolated from a dental office

Detalhes bibliográficos
Autor(a) principal: Pimenta-Rodrigues, M. V. [UNESP]
Data de Publicação: 2008
Outros Autores: Fusco-Almeida, A. M., Bertoni, B. W., Pietro, R. C.L.R. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-91992008000400012
http://hdl.handle.net/11449/225370
Resumo: The present study aimed to analyze the genetic similarity between genomic profiles of thirteen Klebsiella oxytoca and seven Klebsiella pneumoniae samples isolated from two different collections carried out in different places of dental offices. Random amplified polymorphic DNA (RAPD) technique and similarity coefficients (calculated by Sorensen-Dice and simple matching) were applied to determine their genetic profile of randomic DNA sequences. The majority of the isolates of K. pneumoniae and K. oxytoca presented similar coefficient values (gt; 0.80). Thus, it was possible to identify that strain dissemination occurred mainly via the hands of the surgeon-dentists and, finally, to determine the genetic similarity of the strains from dental office environments.
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spelling Assessment of genetic relationship between klebsiella pneumoniae and klebsiella oxytoca samples isolated from a dental officeDental officeKlebsiella oxytocaKlebsiella pneumoniaeRAPDThe present study aimed to analyze the genetic similarity between genomic profiles of thirteen Klebsiella oxytoca and seven Klebsiella pneumoniae samples isolated from two different collections carried out in different places of dental offices. Random amplified polymorphic DNA (RAPD) technique and similarity coefficients (calculated by Sorensen-Dice and simple matching) were applied to determine their genetic profile of randomic DNA sequences. The majority of the isolates of K. pneumoniae and K. oxytoca presented similar coefficient values (gt; 0.80). Thus, it was possible to identify that strain dissemination occurred mainly via the hands of the surgeon-dentists and, finally, to determine the genetic similarity of the strains from dental office environments.Unit of Medicinal Plant and Microorganism Biotechnology Ribeirâo Preto University, Ribeirâo Preto, Sao Paulo StatePharmaceutical Sciences Course Ribeirâo Preto University, Ribeirâo Preto, Sâo Paulo StateSchool of Pharmaceutical Sciences Sâo Paulo State University UNESP, Araraquara, Sâo Paulo StateDepartment of Tropical Diseases Sâo Paulo State University UNESP, Botucatu, Sâo Paulo StateDepartamento de Fármacos e Medicamentos Faculdade de Ciências Farmacêuticas de Araraquara UNESP Araraquara, SP, 14801-902, BrasilSchool of Pharmaceutical Sciences Sâo Paulo State University UNESP, Araraquara, Sâo Paulo StateDepartment of Tropical Diseases Sâo Paulo State University UNESP, Botucatu, Sâo Paulo StateDepartamento de Fármacos e Medicamentos Faculdade de Ciências Farmacêuticas de Araraquara UNESP Araraquara, SP, 14801-902, BrasilRibeirâo Preto UniversityUniversidade Estadual Paulista (UNESP)Pimenta-Rodrigues, M. V. [UNESP]Fusco-Almeida, A. M.Bertoni, B. W.Pietro, R. C.L.R. [UNESP]2022-04-28T20:48:10Z2022-04-28T20:48:10Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article703-718http://dx.doi.org/10.1590/S1678-91992008000400012Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 14, n. 4, p. 703-718, 2008.1678-9199http://hdl.handle.net/11449/22537010.1590/S1678-919920080004000122-s2.0-58049144493Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2024-08-15T15:23:16Zoai:repositorio.unesp.br:11449/225370Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:23:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Assessment of genetic relationship between klebsiella pneumoniae and klebsiella oxytoca samples isolated from a dental office
title Assessment of genetic relationship between klebsiella pneumoniae and klebsiella oxytoca samples isolated from a dental office
spellingShingle Assessment of genetic relationship between klebsiella pneumoniae and klebsiella oxytoca samples isolated from a dental office
Pimenta-Rodrigues, M. V. [UNESP]
Dental office
Klebsiella oxytoca
Klebsiella pneumoniae
RAPD
title_short Assessment of genetic relationship between klebsiella pneumoniae and klebsiella oxytoca samples isolated from a dental office
title_full Assessment of genetic relationship between klebsiella pneumoniae and klebsiella oxytoca samples isolated from a dental office
title_fullStr Assessment of genetic relationship between klebsiella pneumoniae and klebsiella oxytoca samples isolated from a dental office
title_full_unstemmed Assessment of genetic relationship between klebsiella pneumoniae and klebsiella oxytoca samples isolated from a dental office
title_sort Assessment of genetic relationship between klebsiella pneumoniae and klebsiella oxytoca samples isolated from a dental office
author Pimenta-Rodrigues, M. V. [UNESP]
author_facet Pimenta-Rodrigues, M. V. [UNESP]
Fusco-Almeida, A. M.
Bertoni, B. W.
Pietro, R. C.L.R. [UNESP]
author_role author
author2 Fusco-Almeida, A. M.
Bertoni, B. W.
Pietro, R. C.L.R. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Ribeirâo Preto University
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Pimenta-Rodrigues, M. V. [UNESP]
Fusco-Almeida, A. M.
Bertoni, B. W.
Pietro, R. C.L.R. [UNESP]
dc.subject.por.fl_str_mv Dental office
Klebsiella oxytoca
Klebsiella pneumoniae
RAPD
topic Dental office
Klebsiella oxytoca
Klebsiella pneumoniae
RAPD
description The present study aimed to analyze the genetic similarity between genomic profiles of thirteen Klebsiella oxytoca and seven Klebsiella pneumoniae samples isolated from two different collections carried out in different places of dental offices. Random amplified polymorphic DNA (RAPD) technique and similarity coefficients (calculated by Sorensen-Dice and simple matching) were applied to determine their genetic profile of randomic DNA sequences. The majority of the isolates of K. pneumoniae and K. oxytoca presented similar coefficient values (gt; 0.80). Thus, it was possible to identify that strain dissemination occurred mainly via the hands of the surgeon-dentists and, finally, to determine the genetic similarity of the strains from dental office environments.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
2022-04-28T20:48:10Z
2022-04-28T20:48:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992008000400012
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 14, n. 4, p. 703-718, 2008.
1678-9199
http://hdl.handle.net/11449/225370
10.1590/S1678-91992008000400012
2-s2.0-58049144493
url http://dx.doi.org/10.1590/S1678-91992008000400012
http://hdl.handle.net/11449/225370
identifier_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 14, n. 4, p. 703-718, 2008.
1678-9199
10.1590/S1678-91992008000400012
2-s2.0-58049144493
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 703-718
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128195170402304

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