Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae)
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1517-83822011000200046 http://hdl.handle.net/11449/26644 |
Resumo: | Wolbachia are endosymbiont bacteria of the family Rickettsiacea that are widespread in invertebrates and occur between 20% and 60% of Neotropical insects. These bacteria are responsible for reproductive phenomena such as cytoplasmic incompatibility, male killing, feminization and parthenogenesis. Supergroups A and B of Wolbachia are common in insects and can be identified using primers for 16S rDNA, ftsZ and wsp; these primers vary in their ability to detect Wolbachia. The ftsZ primer was the first primer used to detect Wolbachia in Anastrepha fruit flies. The primers for 16S rDNA, ftsZ and wsp and the corresponding PCR conditions have been optimized to study the distribution of Wolbachia and their effect on the biology of Anastrepha in Brazil. In this work, we examined the ability of these primers to detect Wolbachia in Anastrepha populations from three regions in the State of São Paulo, southeastern Brazil. All of the samples were positive for Wolbachia supergroup A when screened with primers for 16S A rDNA and wsp A; the wsp B primer also gave a positive result, indicating cross-reactivity. The ftsZ primer showed a poor ability to detect Wolbachia in Anastrepha and generated false negatives in 44.9% of the samples. These findings indicate that reliable PCR detection of Wolbachia requires the use of primers for 16S rDNA and wsp to avoid cross-reactions and false negatives, and that the ftsZ primer needs to be redesigned to improve its selectivity. |
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Repositório Institucional da UNESP |
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spelling |
Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae)Anastrephacross reactionftsZwspWolbachia are endosymbiont bacteria of the family Rickettsiacea that are widespread in invertebrates and occur between 20% and 60% of Neotropical insects. These bacteria are responsible for reproductive phenomena such as cytoplasmic incompatibility, male killing, feminization and parthenogenesis. Supergroups A and B of Wolbachia are common in insects and can be identified using primers for 16S rDNA, ftsZ and wsp; these primers vary in their ability to detect Wolbachia. The ftsZ primer was the first primer used to detect Wolbachia in Anastrepha fruit flies. The primers for 16S rDNA, ftsZ and wsp and the corresponding PCR conditions have been optimized to study the distribution of Wolbachia and their effect on the biology of Anastrepha in Brazil. In this work, we examined the ability of these primers to detect Wolbachia in Anastrepha populations from three regions in the State of São Paulo, southeastern Brazil. All of the samples were positive for Wolbachia supergroup A when screened with primers for 16S A rDNA and wsp A; the wsp B primer also gave a positive result, indicating cross-reactivity. The ftsZ primer showed a poor ability to detect Wolbachia in Anastrepha and generated false negatives in 44.9% of the samples. These findings indicate that reliable PCR detection of Wolbachia requires the use of primers for 16S rDNA and wsp to avoid cross-reactions and false negatives, and that the ftsZ primer needs to be redesigned to improve its selectivity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Departamento de GenéticaUniversidade de São Paulo Instituto de Biociências Departamento de Genética e Biologia EvolutivaUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Departamento de GenéticaSociedade Brasileira de MicrobiologiaUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Marcon, Helena Sanches [UNESP]Coscrato, Virgínia Elias [UNESP]Selivon, DenisePerondini, André Luiz ParanhosMarino, Celso Luis [UNESP]2014-05-20T15:07:44Z2014-05-20T15:07:44Z2011-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article778-785application/pdfhttp://dx.doi.org/10.1590/S1517-83822011000200046Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 2, p. 778-785, 2011.1517-8382http://hdl.handle.net/11449/2664410.1590/S1517-83822011000200046S1517-83822011000200046WOS:000291614000046S1517-83822011000200046.pdf01653487382083190000-0003-4524-954XSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2023-11-07T06:11:35Zoai:repositorio.unesp.br:11449/26644Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:05:05.847221Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae) |
title |
Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae) |
spellingShingle |
Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae) Marcon, Helena Sanches [UNESP] Anastrepha cross reaction ftsZ wsp |
title_short |
Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae) |
title_full |
Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae) |
title_fullStr |
Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae) |
title_full_unstemmed |
Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae) |
title_sort |
Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae) |
author |
Marcon, Helena Sanches [UNESP] |
author_facet |
Marcon, Helena Sanches [UNESP] Coscrato, Virgínia Elias [UNESP] Selivon, Denise Perondini, André Luiz Paranhos Marino, Celso Luis [UNESP] |
author_role |
author |
author2 |
Coscrato, Virgínia Elias [UNESP] Selivon, Denise Perondini, André Luiz Paranhos Marino, Celso Luis [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Marcon, Helena Sanches [UNESP] Coscrato, Virgínia Elias [UNESP] Selivon, Denise Perondini, André Luiz Paranhos Marino, Celso Luis [UNESP] |
dc.subject.por.fl_str_mv |
Anastrepha cross reaction ftsZ wsp |
topic |
Anastrepha cross reaction ftsZ wsp |
description |
Wolbachia are endosymbiont bacteria of the family Rickettsiacea that are widespread in invertebrates and occur between 20% and 60% of Neotropical insects. These bacteria are responsible for reproductive phenomena such as cytoplasmic incompatibility, male killing, feminization and parthenogenesis. Supergroups A and B of Wolbachia are common in insects and can be identified using primers for 16S rDNA, ftsZ and wsp; these primers vary in their ability to detect Wolbachia. The ftsZ primer was the first primer used to detect Wolbachia in Anastrepha fruit flies. The primers for 16S rDNA, ftsZ and wsp and the corresponding PCR conditions have been optimized to study the distribution of Wolbachia and their effect on the biology of Anastrepha in Brazil. In this work, we examined the ability of these primers to detect Wolbachia in Anastrepha populations from three regions in the State of São Paulo, southeastern Brazil. All of the samples were positive for Wolbachia supergroup A when screened with primers for 16S A rDNA and wsp A; the wsp B primer also gave a positive result, indicating cross-reactivity. The ftsZ primer showed a poor ability to detect Wolbachia in Anastrepha and generated false negatives in 44.9% of the samples. These findings indicate that reliable PCR detection of Wolbachia requires the use of primers for 16S rDNA and wsp to avoid cross-reactions and false negatives, and that the ftsZ primer needs to be redesigned to improve its selectivity. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-01 2014-05-20T15:07:44Z 2014-05-20T15:07:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1517-83822011000200046 Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 2, p. 778-785, 2011. 1517-8382 http://hdl.handle.net/11449/26644 10.1590/S1517-83822011000200046 S1517-83822011000200046 WOS:000291614000046 S1517-83822011000200046.pdf 0165348738208319 0000-0003-4524-954X |
url |
http://dx.doi.org/10.1590/S1517-83822011000200046 http://hdl.handle.net/11449/26644 |
identifier_str_mv |
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 2, p. 778-785, 2011. 1517-8382 10.1590/S1517-83822011000200046 S1517-83822011000200046 WOS:000291614000046 S1517-83822011000200046.pdf 0165348738208319 0000-0003-4524-954X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology 1.810 0,630 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
778-785 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128752997105664 |