Poly-ε-caprolactone nanoparticles loaded with 4-nerolidylcatechol (4-nc) for growth inhibition of microsporum canis

• Autor(a) principal: Greatti, Vanessa Raquel [UNESP]
• Data de Publicação: 2020
• Outros Autores: Oda, Fernando [UNESP], Sorrechia, Rodrigo [UNESP], Kapp, Bárbara Regina [UNESP], Seraphim, Carolina Manzato [UNESP], Weckwerth, Ana Carolina Villas Bôas, Chorilli, Marlus [UNESP], Da Silva, Patrícia Bento [UNESP], Eloy, Josimar O., Kogan, Marcelo J., Morales, Javier O., Rodrigues Pietro, Rosemeire Cristina Linhari [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.3390/antibiotics9120894; http://hdl.handle.net/11449/206965
• topic: 4-Nerolidylcatechol; Antifungals; Microsporum canis; Nanoparticles; Nanoprecipitation; Polycaprolactone

Dermatophyte fungal infections are difficult to treat because they need long-term treatments. 4-Nerolidylcatechol (4-NC) is a compound found in Piper umbellatum that has been reported to demonstrate significant antifungal activity, but is easily oxidizable. Due to this characteristic, the incorporation in nanostructured systems represents a strategy to guarantee the compound’s stability compared to the isolated form and the possibility of improving antifungal activity. The objective of this study was to incorporate 4-NC into polymeric nanoparticles to evaluate, in vitro and in vivo, the growth inhibition of Microsporum canis. 4-NC was isolated from fresh leaves of P. umbellatum, and polymer nanoparticles of polycaprolactone were developed by nanoprecipitation using a 1:5 weight ratio (drug:polymer). Nanoparticles exhibited excellent encapsulation efficiency, and the antifungal activity was observed in nanoparticles with 4-NC incorporated. Polymeric nanoparticles can be a strategy employed for decreased cytotoxicity, increasing the stability and solubility of substances, as well as improving the efficacy of 4-NC.