HOX genes: potential candidates for the progression of laryngeal squamous cell carcinoma
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s13277-016-5356-8 http://hdl.handle.net/11449/176945 |
Resumo: | Laryngeal squamous cell carcinoma (LSCC) is a very aggressive cancer, considered to be a subtype of the head and neck squamous cell carcinoma (HNSCC). Despite significant advances in the understanding and treatment of cancer, prognosis of patients with LSCC has not improved recently. In the present study, we sought to understand better the genetic mechanisms underlying LSCC development. Thirty-two tumor samples were collected from patients undergoing surgical resection of LSCC. The samples were submitted to whole-genome cDNA microarray analysis aiming to identify genetic targets in LSCC. We also employed bioinformatic approaches to expand our findings using the TCGA database and further performed functional assays, using human HNSCC cell lines, to evaluate viability, cell proliferation, and cell migration after silencing of selected genes. Eight members of the homeobox gene family (HOX) were identified to be overexpressed in LSCC samples when compared to normal larynx tissue. Quantitative RT-PCR analysis validated the overexpression of HOX gene family members in LSCC. Receiver operating characteristic (ROC) statistical method curve showed that the expression level of seven members of HOX gene family can distinguish tumor from nontumor tissue. Correlation analysis of clinical and gene expression data revealed that HOXC8 and HOXD11 genes were associated with the differentiation degree of tumors and regional lymph node metastases, respectively. Additionally, siRNA assays confirmed that HOXC8, HOXD10, and HOXD11 genes might be critical for cell colony proliferation and cell migration. According to our findings, several members of the HOX genes were overexpressed in LSCC samples and seem to be required in biological processes involved in tumor development. This suggests that HOX genes might play a critical role in the physiopathology of LSCC tumors. |
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HOX genes: potential candidates for the progression of laryngeal squamous cell carcinomaCell migrationGene regulationHOX genesLarynx squamous cell carcinomaLaryngeal squamous cell carcinoma (LSCC) is a very aggressive cancer, considered to be a subtype of the head and neck squamous cell carcinoma (HNSCC). Despite significant advances in the understanding and treatment of cancer, prognosis of patients with LSCC has not improved recently. In the present study, we sought to understand better the genetic mechanisms underlying LSCC development. Thirty-two tumor samples were collected from patients undergoing surgical resection of LSCC. The samples were submitted to whole-genome cDNA microarray analysis aiming to identify genetic targets in LSCC. We also employed bioinformatic approaches to expand our findings using the TCGA database and further performed functional assays, using human HNSCC cell lines, to evaluate viability, cell proliferation, and cell migration after silencing of selected genes. Eight members of the homeobox gene family (HOX) were identified to be overexpressed in LSCC samples when compared to normal larynx tissue. Quantitative RT-PCR analysis validated the overexpression of HOX gene family members in LSCC. Receiver operating characteristic (ROC) statistical method curve showed that the expression level of seven members of HOX gene family can distinguish tumor from nontumor tissue. Correlation analysis of clinical and gene expression data revealed that HOXC8 and HOXD11 genes were associated with the differentiation degree of tumors and regional lymph node metastases, respectively. Additionally, siRNA assays confirmed that HOXC8, HOXD10, and HOXD11 genes might be critical for cell colony proliferation and cell migration. According to our findings, several members of the HOX genes were overexpressed in LSCC samples and seem to be required in biological processes involved in tumor development. This suggests that HOX genes might play a critical role in the physiopathology of LSCC tumors.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Genetics Ribeir�o Preto Medical School University of S�o Paulo, Avenida Bandeirantes 3900, Monte AlegreNational Institute of Science and Technology Stem Cell and Cell Therapy and Center for Cell Based Therapy, Rua Tenente Cat�o Roxo, 2501, Monte AlegreDepartment of Technology College of Agriculture and Veterinary Sciences UNESPCenter for Integrative Systems Biology CISBi NAP/USP, Rua Cat�o Roxo, 2501, Monte AlegreDepartment of Pathology Ribeir�o Preto Medical School University of S�o Paulo, Avenida Bandeirantes 3900, Monte AlegreDepartment of Pathology Memorial Sloan-Kettering Cancer Center, 1275 York AvenueDepartment of Ophthalmology Otorhinolaryngology and Head and Neck Surgery Ribeir�o Preto Medical School University of S�o Paulo, Avenida Bandeirantes 3900, Monte AlegreUniversity of Centro-Oeste, Rua Padre Salvador, 875, Santa CruzCentro Regional de Hemoterapia de Ribeir�o Preto, Rua Cat�o Roxo, 2501, Monte AlegreDepartment of Technology College of Agriculture and Veterinary Sciences UNESPCNPq: 140427/2010-4FAPESP: 2012/00588-5FAPESP: 2013/08135-2CNPq: 559809/2009-3University of S�o PauloStem Cell and Cell Therapy and Center for Cell Based TherapyUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Memorial Sloan-Kettering Cancer CenterUniversity of Centro-OesteCentro Regional de Hemoterapia de Ribeir�o Pretode Barros e Lima Bueno, RafaelaRam�o, AnelisaPinheiro, Daniel Guariz [UNESP]Alves, Cleidson PaduaKannen, ViniciusJungbluth, Achim Ade Ara�jo, Luiza FerreiraMuys, Bruna RodriguesFonseca, Aline SimonetiPla�a, Jessica RodriguesPanepucci, Rodrigo AlexandreNeder, LucianoSaggioro, Fabiano PMamede, Rui Celso M.Figueiredo, David Livingstone AlvesSilva, Wilson Ara�jo2018-12-11T17:23:12Z2018-12-11T17:23:12Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15087-15096http://dx.doi.org/10.1007/s13277-016-5356-8Tumor Biology, v. 37, n. 11, p. 15087-15096, 2016.1423-03801010-4283http://hdl.handle.net/11449/17694510.1007/s13277-016-5356-82-s2.0-84988723057Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTumor Biology1,1491,149info:eu-repo/semantics/openAccess2021-10-23T17:16:43Zoai:repositorio.unesp.br:11449/176945Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:16:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
HOX genes: potential candidates for the progression of laryngeal squamous cell carcinoma |
title |
HOX genes: potential candidates for the progression of laryngeal squamous cell carcinoma |
spellingShingle |
HOX genes: potential candidates for the progression of laryngeal squamous cell carcinoma de Barros e Lima Bueno, Rafaela Cell migration Gene regulation HOX genes Larynx squamous cell carcinoma |
title_short |
HOX genes: potential candidates for the progression of laryngeal squamous cell carcinoma |
title_full |
HOX genes: potential candidates for the progression of laryngeal squamous cell carcinoma |
title_fullStr |
HOX genes: potential candidates for the progression of laryngeal squamous cell carcinoma |
title_full_unstemmed |
HOX genes: potential candidates for the progression of laryngeal squamous cell carcinoma |
title_sort |
HOX genes: potential candidates for the progression of laryngeal squamous cell carcinoma |
author |
de Barros e Lima Bueno, Rafaela |
author_facet |
de Barros e Lima Bueno, Rafaela Ram�o, Anelisa Pinheiro, Daniel Guariz [UNESP] Alves, Cleidson Padua Kannen, Vinicius Jungbluth, Achim A de Ara�jo, Luiza Ferreira Muys, Bruna Rodrigues Fonseca, Aline Simoneti Pla�a, Jessica Rodrigues Panepucci, Rodrigo Alexandre Neder, Luciano Saggioro, Fabiano P Mamede, Rui Celso M. Figueiredo, David Livingstone Alves Silva, Wilson Ara�jo |
author_role |
author |
author2 |
Ram�o, Anelisa Pinheiro, Daniel Guariz [UNESP] Alves, Cleidson Padua Kannen, Vinicius Jungbluth, Achim A de Ara�jo, Luiza Ferreira Muys, Bruna Rodrigues Fonseca, Aline Simoneti Pla�a, Jessica Rodrigues Panepucci, Rodrigo Alexandre Neder, Luciano Saggioro, Fabiano P Mamede, Rui Celso M. Figueiredo, David Livingstone Alves Silva, Wilson Ara�jo |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of S�o Paulo Stem Cell and Cell Therapy and Center for Cell Based Therapy Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Memorial Sloan-Kettering Cancer Center University of Centro-Oeste Centro Regional de Hemoterapia de Ribeir�o Preto |
dc.contributor.author.fl_str_mv |
de Barros e Lima Bueno, Rafaela Ram�o, Anelisa Pinheiro, Daniel Guariz [UNESP] Alves, Cleidson Padua Kannen, Vinicius Jungbluth, Achim A de Ara�jo, Luiza Ferreira Muys, Bruna Rodrigues Fonseca, Aline Simoneti Pla�a, Jessica Rodrigues Panepucci, Rodrigo Alexandre Neder, Luciano Saggioro, Fabiano P Mamede, Rui Celso M. Figueiredo, David Livingstone Alves Silva, Wilson Ara�jo |
dc.subject.por.fl_str_mv |
Cell migration Gene regulation HOX genes Larynx squamous cell carcinoma |
topic |
Cell migration Gene regulation HOX genes Larynx squamous cell carcinoma |
description |
Laryngeal squamous cell carcinoma (LSCC) is a very aggressive cancer, considered to be a subtype of the head and neck squamous cell carcinoma (HNSCC). Despite significant advances in the understanding and treatment of cancer, prognosis of patients with LSCC has not improved recently. In the present study, we sought to understand better the genetic mechanisms underlying LSCC development. Thirty-two tumor samples were collected from patients undergoing surgical resection of LSCC. The samples were submitted to whole-genome cDNA microarray analysis aiming to identify genetic targets in LSCC. We also employed bioinformatic approaches to expand our findings using the TCGA database and further performed functional assays, using human HNSCC cell lines, to evaluate viability, cell proliferation, and cell migration after silencing of selected genes. Eight members of the homeobox gene family (HOX) were identified to be overexpressed in LSCC samples when compared to normal larynx tissue. Quantitative RT-PCR analysis validated the overexpression of HOX gene family members in LSCC. Receiver operating characteristic (ROC) statistical method curve showed that the expression level of seven members of HOX gene family can distinguish tumor from nontumor tissue. Correlation analysis of clinical and gene expression data revealed that HOXC8 and HOXD11 genes were associated with the differentiation degree of tumors and regional lymph node metastases, respectively. Additionally, siRNA assays confirmed that HOXC8, HOXD10, and HOXD11 genes might be critical for cell colony proliferation and cell migration. According to our findings, several members of the HOX genes were overexpressed in LSCC samples and seem to be required in biological processes involved in tumor development. This suggests that HOX genes might play a critical role in the physiopathology of LSCC tumors. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-12-11T17:23:12Z 2018-12-11T17:23:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s13277-016-5356-8 Tumor Biology, v. 37, n. 11, p. 15087-15096, 2016. 1423-0380 1010-4283 http://hdl.handle.net/11449/176945 10.1007/s13277-016-5356-8 2-s2.0-84988723057 |
url |
http://dx.doi.org/10.1007/s13277-016-5356-8 http://hdl.handle.net/11449/176945 |
identifier_str_mv |
Tumor Biology, v. 37, n. 11, p. 15087-15096, 2016. 1423-0380 1010-4283 10.1007/s13277-016-5356-8 2-s2.0-84988723057 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Tumor Biology 1,149 1,149 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
15087-15096 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965067864702976 |