Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/microorganisms8020171 http://hdl.handle.net/11449/196681 |
Resumo: | Since no recent data characterizing Shiga toxin-producing E. coli (STEC) from human infections in Brazil are available, the present study aimed to investigate serotypes, stx genotypes, and accessory virulence genes, and also to perform pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) of 43 STEC strains recovered from 2007 to 2017. Twenty-one distinct serotypes were found, with serotype O111:H8 being the most common. However, serotypes less frequently reported in human diseases were also found and included a hybrid STEC/ETEC O100:H25 clone. The majority of the strains carried stxla as the sole stx genotype and were positive for the eae gene. Regarding the occurrence of 28 additional virulence genes associated with plasmids and pathogenicity islands, a diversity of profiles was found especially among the eae-harboring strains, which had combinations of markers composed of up to 12 distinct genes. Although PFGE analysis demonstrated genetic diversity between serotypes such as O157:H7, O111:H8, O26:H11, O118:H16, and O123:H2, high genetic relatedness was found for strains of serotypes O24:H4 and O145:H34. MLST allowed the identification of 17 distinct sequence types (STs) with ST 16 and 21 being the most common ones. Thirty-five percent of the strains studied were not typeable by the currently used MLST approach, suggesting new STs. Although STEC 0111:H8 remains the leading serotype in Brazil, a diversity of other serotypes, some carrying virulence genes and belonging to STs incriminated as causing severe disease, were found in this study. Further studies are needed to determine whether they have any epidemiological relevance. |
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Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in BrazilSTECserotypesvirulence markersmolecular typingMLSTSince no recent data characterizing Shiga toxin-producing E. coli (STEC) from human infections in Brazil are available, the present study aimed to investigate serotypes, stx genotypes, and accessory virulence genes, and also to perform pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) of 43 STEC strains recovered from 2007 to 2017. Twenty-one distinct serotypes were found, with serotype O111:H8 being the most common. However, serotypes less frequently reported in human diseases were also found and included a hybrid STEC/ETEC O100:H25 clone. The majority of the strains carried stxla as the sole stx genotype and were positive for the eae gene. Regarding the occurrence of 28 additional virulence genes associated with plasmids and pathogenicity islands, a diversity of profiles was found especially among the eae-harboring strains, which had combinations of markers composed of up to 12 distinct genes. Although PFGE analysis demonstrated genetic diversity between serotypes such as O157:H7, O111:H8, O26:H11, O118:H16, and O123:H2, high genetic relatedness was found for strains of serotypes O24:H4 and O145:H34. MLST allowed the identification of 17 distinct sequence types (STs) with ST 16 and 21 being the most common ones. Thirty-five percent of the strains studied were not typeable by the currently used MLST approach, suggesting new STs. Although STEC 0111:H8 remains the leading serotype in Brazil, a diversity of other serotypes, some carrying virulence genes and belonging to STs incriminated as causing severe disease, were found in this study. Further studies are needed to determine whether they have any epidemiological relevance.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Adolfo Lutz Inst, Ctr Bacteriol, Natl Reference Lab STEC Infect & HUS, BR-01246000 Sao Paulo, SP, BrazilUniv Estadual Paulista, Dept Microbiol & Imunol, Inst Biociencias, BR-18618970 Botucatu, SP, BrazilUniv Fed Sao Paulo, Dept Microbiol, Imunol, Parasitol,Escola Paulista Med, BR-04023062 Sao Paulo, SP, BrazilUniv Estadual Paulista, Dept Microbiol & Imunol, Inst Biociencias, BR-18618970 Botucatu, SP, BrazilFAPESP: 2017/00411-1FAPESP: 2014/20354-4MdpiAdolfo Lutz InstUniversidade Estadual Paulista (Unesp)Universidade Federal de São Paulo (UNIFESP)Ferreira Cavalcanti, Adriene MariaHernandes, Rodrigo Tavanelli [UNESP]Takagi, Elizabeth HarummyyCabilio Guth, Beatriz ErnestinaOri, Erica de LimaSchicariol Pinheiro, Sandra ReginaAndrade, Tania Sueli deOliveira, Samara Louzada [UNESP]Cergole-Novella, Maria CeciliaFrancisco, Gabriela RodriguesSantos, Luis Fernando dos2020-12-10T19:52:52Z2020-12-10T19:52:52Z2020-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17http://dx.doi.org/10.3390/microorganisms8020171Microorganisms. Basel: Mdpi, v. 8, n. 2, 17 p., 2020.http://hdl.handle.net/11449/19668110.3390/microorganisms8020171WOS:00051961820002783769741155985840000-0001-6695-6003Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroorganismsinfo:eu-repo/semantics/openAccess2021-10-23T09:55:11Zoai:repositorio.unesp.br:11449/196681Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:56:02.074984Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil |
title |
Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil |
spellingShingle |
Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil Ferreira Cavalcanti, Adriene Maria STEC serotypes virulence markers molecular typing MLST |
title_short |
Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil |
title_full |
Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil |
title_fullStr |
Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil |
title_full_unstemmed |
Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil |
title_sort |
Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil |
author |
Ferreira Cavalcanti, Adriene Maria |
author_facet |
Ferreira Cavalcanti, Adriene Maria Hernandes, Rodrigo Tavanelli [UNESP] Takagi, Elizabeth Harummyy Cabilio Guth, Beatriz Ernestina Ori, Erica de Lima Schicariol Pinheiro, Sandra Regina Andrade, Tania Sueli de Oliveira, Samara Louzada [UNESP] Cergole-Novella, Maria Cecilia Francisco, Gabriela Rodrigues Santos, Luis Fernando dos |
author_role |
author |
author2 |
Hernandes, Rodrigo Tavanelli [UNESP] Takagi, Elizabeth Harummyy Cabilio Guth, Beatriz Ernestina Ori, Erica de Lima Schicariol Pinheiro, Sandra Regina Andrade, Tania Sueli de Oliveira, Samara Louzada [UNESP] Cergole-Novella, Maria Cecilia Francisco, Gabriela Rodrigues Santos, Luis Fernando dos |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Adolfo Lutz Inst Universidade Estadual Paulista (Unesp) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Ferreira Cavalcanti, Adriene Maria Hernandes, Rodrigo Tavanelli [UNESP] Takagi, Elizabeth Harummyy Cabilio Guth, Beatriz Ernestina Ori, Erica de Lima Schicariol Pinheiro, Sandra Regina Andrade, Tania Sueli de Oliveira, Samara Louzada [UNESP] Cergole-Novella, Maria Cecilia Francisco, Gabriela Rodrigues Santos, Luis Fernando dos |
dc.subject.por.fl_str_mv |
STEC serotypes virulence markers molecular typing MLST |
topic |
STEC serotypes virulence markers molecular typing MLST |
description |
Since no recent data characterizing Shiga toxin-producing E. coli (STEC) from human infections in Brazil are available, the present study aimed to investigate serotypes, stx genotypes, and accessory virulence genes, and also to perform pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) of 43 STEC strains recovered from 2007 to 2017. Twenty-one distinct serotypes were found, with serotype O111:H8 being the most common. However, serotypes less frequently reported in human diseases were also found and included a hybrid STEC/ETEC O100:H25 clone. The majority of the strains carried stxla as the sole stx genotype and were positive for the eae gene. Regarding the occurrence of 28 additional virulence genes associated with plasmids and pathogenicity islands, a diversity of profiles was found especially among the eae-harboring strains, which had combinations of markers composed of up to 12 distinct genes. Although PFGE analysis demonstrated genetic diversity between serotypes such as O157:H7, O111:H8, O26:H11, O118:H16, and O123:H2, high genetic relatedness was found for strains of serotypes O24:H4 and O145:H34. MLST allowed the identification of 17 distinct sequence types (STs) with ST 16 and 21 being the most common ones. Thirty-five percent of the strains studied were not typeable by the currently used MLST approach, suggesting new STs. Although STEC 0111:H8 remains the leading serotype in Brazil, a diversity of other serotypes, some carrying virulence genes and belonging to STs incriminated as causing severe disease, were found in this study. Further studies are needed to determine whether they have any epidemiological relevance. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T19:52:52Z 2020-12-10T19:52:52Z 2020-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/microorganisms8020171 Microorganisms. Basel: Mdpi, v. 8, n. 2, 17 p., 2020. http://hdl.handle.net/11449/196681 10.3390/microorganisms8020171 WOS:000519618200027 8376974115598584 0000-0001-6695-6003 |
url |
http://dx.doi.org/10.3390/microorganisms8020171 http://hdl.handle.net/11449/196681 |
identifier_str_mv |
Microorganisms. Basel: Mdpi, v. 8, n. 2, 17 p., 2020. 10.3390/microorganisms8020171 WOS:000519618200027 8376974115598584 0000-0001-6695-6003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microorganisms |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17 |
dc.publisher.none.fl_str_mv |
Mdpi |
publisher.none.fl_str_mv |
Mdpi |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128723274170368 |