Investigations of biofilms formed on silica in contact with aqueous formulations containing laccase and maltodextrin
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s40563-016-0059-3 http://hdl.handle.net/11449/169550 |
Resumo: | The formation of nano-scale biofilms on hydrophilic silica surfaces from aqueous polypeptide/polysaccharide mixtures containing laccase and maltodextrin was investigated in situ with quartz crystal microbalance with dissipation monitoring (QCM-D). Surface analysis techniques such as X-ray photoelectron spectroscopy (XPS), optically stimulated electron emission (OSEE) and atomic force microscopy (AFM) were applied for characterizing the resulting layers obtained after periods varying from a few seconds to several hours of contact between the substrate and the biopolymers formulation. The biofilm formation in contact with the aqueous laccase/maltodextrin suspension was studied at pH level 4.75, under conditions close to the isoelectric point of the enzyme. The few nanometers rough biofilms obtained were composed of a laccase/maltodextrin mixture, and their thickness was observed to steadily increase during 4 h of contact with the aqueous mixture of biopolymers. Remarkably, the still adhesive films obtained after 1 h of contact with aqueous polypeptide/polysaccharide mixture resisted a 30 min rinsing with acetate buffer. The biofilms growing process was monitored using OSEE, due to the effected attenuation of the UV-induced electron emission from the SiO2/Si substrate, which was found to be more pronounced than the attenuation of the photoelectrons from the substrate which contribute to the XPS signals. Layers as thin as 1 nm were detected by the OSEE. |
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Investigations of biofilms formed on silica in contact with aqueous formulations containing laccase and maltodextrinAdhesion of biofilmsBiopolymer adsorptionLaccase maltodextrin mixtureSurface analysis and monitoringThe formation of nano-scale biofilms on hydrophilic silica surfaces from aqueous polypeptide/polysaccharide mixtures containing laccase and maltodextrin was investigated in situ with quartz crystal microbalance with dissipation monitoring (QCM-D). Surface analysis techniques such as X-ray photoelectron spectroscopy (XPS), optically stimulated electron emission (OSEE) and atomic force microscopy (AFM) were applied for characterizing the resulting layers obtained after periods varying from a few seconds to several hours of contact between the substrate and the biopolymers formulation. The biofilm formation in contact with the aqueous laccase/maltodextrin suspension was studied at pH level 4.75, under conditions close to the isoelectric point of the enzyme. The few nanometers rough biofilms obtained were composed of a laccase/maltodextrin mixture, and their thickness was observed to steadily increase during 4 h of contact with the aqueous mixture of biopolymers. Remarkably, the still adhesive films obtained after 1 h of contact with aqueous polypeptide/polysaccharide mixture resisted a 30 min rinsing with acetate buffer. The biofilms growing process was monitored using OSEE, due to the effected attenuation of the UV-induced electron emission from the SiO2/Si substrate, which was found to be more pronounced than the attenuation of the photoelectrons from the substrate which contribute to the XPS signals. Layers as thin as 1 nm were detected by the OSEE.Pós-Graduação em Ciência e Tecnologia de Materiais (POSMAT) São Paulo State University (UNESP), Av. Eng. Luiz Edmundo Carrijo Coube 14-01Fraunhofer Institute for Manufacturing Technology and Advanced Materials (IFAM), Wiener Straße 12Federal University of Ceará, Av. Da Universidade 2853São Paulo State University (UNESP), Av. Eng. Luiz Edmundo Carrijo Coube 14-01Pós-Graduação em Ciência e Tecnologia de Materiais (POSMAT) São Paulo State University (UNESP), Av. Eng. Luiz Edmundo Carrijo Coube 14-01São Paulo State University (UNESP), Av. Eng. Luiz Edmundo Carrijo Coube 14-01Universidade Estadual Paulista (Unesp)Fraunhofer Institute for Manufacturing Technology and Advanced Materials (IFAM)Federal University of CearáCorrales Ureña, Yendry Regina [UNESP]Gaetjen, LindaNascimento, Matheus VieiraFilho, Paulo Noronha Lisboa [UNESP]Cavalcanti, Welchy LeiteNoeske, Paul-Ludwig MichaelRischka, Klaus2018-12-11T16:46:24Z2018-12-11T16:46:24Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s40563-016-0059-3Applied Adhesion Science, v. 4, n. 1, 2016.2196-4351http://hdl.handle.net/11449/16955010.1186/s40563-016-0059-32-s2.0-850153676992-s2.0-85015367699.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Adhesion Science0,337info:eu-repo/semantics/openAccess2024-01-15T06:17:30Zoai:repositorio.unesp.br:11449/169550Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:00:21.951592Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Investigations of biofilms formed on silica in contact with aqueous formulations containing laccase and maltodextrin |
title |
Investigations of biofilms formed on silica in contact with aqueous formulations containing laccase and maltodextrin |
spellingShingle |
Investigations of biofilms formed on silica in contact with aqueous formulations containing laccase and maltodextrin Corrales Ureña, Yendry Regina [UNESP] Adhesion of biofilms Biopolymer adsorption Laccase maltodextrin mixture Surface analysis and monitoring |
title_short |
Investigations of biofilms formed on silica in contact with aqueous formulations containing laccase and maltodextrin |
title_full |
Investigations of biofilms formed on silica in contact with aqueous formulations containing laccase and maltodextrin |
title_fullStr |
Investigations of biofilms formed on silica in contact with aqueous formulations containing laccase and maltodextrin |
title_full_unstemmed |
Investigations of biofilms formed on silica in contact with aqueous formulations containing laccase and maltodextrin |
title_sort |
Investigations of biofilms formed on silica in contact with aqueous formulations containing laccase and maltodextrin |
author |
Corrales Ureña, Yendry Regina [UNESP] |
author_facet |
Corrales Ureña, Yendry Regina [UNESP] Gaetjen, Linda Nascimento, Matheus Vieira Filho, Paulo Noronha Lisboa [UNESP] Cavalcanti, Welchy Leite Noeske, Paul-Ludwig Michael Rischka, Klaus |
author_role |
author |
author2 |
Gaetjen, Linda Nascimento, Matheus Vieira Filho, Paulo Noronha Lisboa [UNESP] Cavalcanti, Welchy Leite Noeske, Paul-Ludwig Michael Rischka, Klaus |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Fraunhofer Institute for Manufacturing Technology and Advanced Materials (IFAM) Federal University of Ceará |
dc.contributor.author.fl_str_mv |
Corrales Ureña, Yendry Regina [UNESP] Gaetjen, Linda Nascimento, Matheus Vieira Filho, Paulo Noronha Lisboa [UNESP] Cavalcanti, Welchy Leite Noeske, Paul-Ludwig Michael Rischka, Klaus |
dc.subject.por.fl_str_mv |
Adhesion of biofilms Biopolymer adsorption Laccase maltodextrin mixture Surface analysis and monitoring |
topic |
Adhesion of biofilms Biopolymer adsorption Laccase maltodextrin mixture Surface analysis and monitoring |
description |
The formation of nano-scale biofilms on hydrophilic silica surfaces from aqueous polypeptide/polysaccharide mixtures containing laccase and maltodextrin was investigated in situ with quartz crystal microbalance with dissipation monitoring (QCM-D). Surface analysis techniques such as X-ray photoelectron spectroscopy (XPS), optically stimulated electron emission (OSEE) and atomic force microscopy (AFM) were applied for characterizing the resulting layers obtained after periods varying from a few seconds to several hours of contact between the substrate and the biopolymers formulation. The biofilm formation in contact with the aqueous laccase/maltodextrin suspension was studied at pH level 4.75, under conditions close to the isoelectric point of the enzyme. The few nanometers rough biofilms obtained were composed of a laccase/maltodextrin mixture, and their thickness was observed to steadily increase during 4 h of contact with the aqueous mixture of biopolymers. Remarkably, the still adhesive films obtained after 1 h of contact with aqueous polypeptide/polysaccharide mixture resisted a 30 min rinsing with acetate buffer. The biofilms growing process was monitored using OSEE, due to the effected attenuation of the UV-induced electron emission from the SiO2/Si substrate, which was found to be more pronounced than the attenuation of the photoelectrons from the substrate which contribute to the XPS signals. Layers as thin as 1 nm were detected by the OSEE. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T16:46:24Z 2018-12-11T16:46:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s40563-016-0059-3 Applied Adhesion Science, v. 4, n. 1, 2016. 2196-4351 http://hdl.handle.net/11449/169550 10.1186/s40563-016-0059-3 2-s2.0-85015367699 2-s2.0-85015367699.pdf |
url |
http://dx.doi.org/10.1186/s40563-016-0059-3 http://hdl.handle.net/11449/169550 |
identifier_str_mv |
Applied Adhesion Science, v. 4, n. 1, 2016. 2196-4351 10.1186/s40563-016-0059-3 2-s2.0-85015367699 2-s2.0-85015367699.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applied Adhesion Science 0,337 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129481051734016 |