Effects of the solubility of yeast cell wall preparations on their potential prebiotic properties in dogs
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0225659 http://hdl.handle.net/11449/201349 |
Resumo: | Derivatives of yeast cell wall (YCW) have been studied for their potential prebiotic effects. Recently, new purified and soluble preparations have been developed in an attempt to increase their biological actions. Two YCW preparations, one conventional and another with higher solubility of the mannan oligosaccharide fraction, were evaluated on dogs. One food formulation was used, divided into the following treatments: CON–control, without yeast cell wall addition; YCW–addition of 0.3% of a conventional yeas cell wall extract; YCWs–addition of 0.3% of a yeast cell wall extract with high mannan oligosaccharide solubility. Twenty-four beagle dogs were used, eight per food, distributed on a block design. Blocks lasted 32 days, and TNF-a, IL-6, IL-10, ex vivo production of hydrogen peroxide and nitric oxide by peripheral neutrophils and monocytes, phagocytic index, and fecal IgA were evaluated at the beginning and end of each period. Additionally, nutrient digestibility, feces production and quality, and fermentation products were quantified. The results were evaluated by analysis of variance and compared using the Tukey test (P<0.05), using the basal immunological parameters as a covariate. The inclusion of YCWs reduced fat digestibility (P<0.05), increased the concentration of butyrate and putrescine, and reduced lactate in feces (P<0.05), showing that mannan oligosaccharide solubilization resulted in higher fermentation of this compound and altered the metabolism of the gut microbiota. Lower IL-6 on serum was verified for dogs fed the YCWs diet (P<0.05), suggesting a reduction in the inflammatory activity of dogs. Higher phagocytic index was verified for peripheral monocytes after the intake of the YCW food, suggesting better innate immunity. In conclusion, the solubilization of the mannooligosaccharide fraction alters its interaction with gut microbiota and biological actions in animals, although both yeast cell wall preparations exhibited prebiotic effects on dogs. |
id |
UNSP_3a58e43378be24ff32f801421f023f71 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/201349 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effects of the solubility of yeast cell wall preparations on their potential prebiotic properties in dogsDerivatives of yeast cell wall (YCW) have been studied for their potential prebiotic effects. Recently, new purified and soluble preparations have been developed in an attempt to increase their biological actions. Two YCW preparations, one conventional and another with higher solubility of the mannan oligosaccharide fraction, were evaluated on dogs. One food formulation was used, divided into the following treatments: CON–control, without yeast cell wall addition; YCW–addition of 0.3% of a conventional yeas cell wall extract; YCWs–addition of 0.3% of a yeast cell wall extract with high mannan oligosaccharide solubility. Twenty-four beagle dogs were used, eight per food, distributed on a block design. Blocks lasted 32 days, and TNF-a, IL-6, IL-10, ex vivo production of hydrogen peroxide and nitric oxide by peripheral neutrophils and monocytes, phagocytic index, and fecal IgA were evaluated at the beginning and end of each period. Additionally, nutrient digestibility, feces production and quality, and fermentation products were quantified. The results were evaluated by analysis of variance and compared using the Tukey test (P<0.05), using the basal immunological parameters as a covariate. The inclusion of YCWs reduced fat digestibility (P<0.05), increased the concentration of butyrate and putrescine, and reduced lactate in feces (P<0.05), showing that mannan oligosaccharide solubilization resulted in higher fermentation of this compound and altered the metabolism of the gut microbiota. Lower IL-6 on serum was verified for dogs fed the YCWs diet (P<0.05), suggesting a reduction in the inflammatory activity of dogs. Higher phagocytic index was verified for peripheral monocytes after the intake of the YCW food, suggesting better innate immunity. In conclusion, the solubilization of the mannooligosaccharide fraction alters its interaction with gut microbiota and biological actions in animals, although both yeast cell wall preparations exhibited prebiotic effects on dogs.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Veterinary Medicine and Surgery Department College of Agrarian and Veterinarian Sciences (FCAV) São Paulo State University–UNESPBiorigin BrasilPharmacy Faculty Minas Gerais Federal University (UFMG)Veterinary Medicine and Surgery Department College of Agrarian and Veterinarian Sciences (FCAV) São Paulo State University–UNESPCAPES: 001FAPESP: 2017/13623-7CNPq: 47379/ PIBICUniversidade Estadual Paulista (Unesp)Biorigin BrasilUniversidade Federal de Minas Gerais (UFMG)de Souza Theodoro, Stephanie [UNESP]Putarov, Thaila Cristina [UNESP]Tiemi, Caroline [UNESP]Volpe, Lara Mantovani [UNESP]de Oliveira, Carlos Alberto Ferreirade Abreu Glória, Maria BeatrizCarciofi, Aulus Cavalieri [UNESP]2020-12-12T02:30:19Z2020-12-12T02:30:19Z2019-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0225659PLoS ONE, v. 14, n. 11, 2019.1932-6203http://hdl.handle.net/11449/20134910.1371/journal.pone.02256592-s2.0-85075506535Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2024-06-06T14:10:33Zoai:repositorio.unesp.br:11449/201349Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:09:38.278947Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of the solubility of yeast cell wall preparations on their potential prebiotic properties in dogs |
title |
Effects of the solubility of yeast cell wall preparations on their potential prebiotic properties in dogs |
spellingShingle |
Effects of the solubility of yeast cell wall preparations on their potential prebiotic properties in dogs de Souza Theodoro, Stephanie [UNESP] |
title_short |
Effects of the solubility of yeast cell wall preparations on their potential prebiotic properties in dogs |
title_full |
Effects of the solubility of yeast cell wall preparations on their potential prebiotic properties in dogs |
title_fullStr |
Effects of the solubility of yeast cell wall preparations on their potential prebiotic properties in dogs |
title_full_unstemmed |
Effects of the solubility of yeast cell wall preparations on their potential prebiotic properties in dogs |
title_sort |
Effects of the solubility of yeast cell wall preparations on their potential prebiotic properties in dogs |
author |
de Souza Theodoro, Stephanie [UNESP] |
author_facet |
de Souza Theodoro, Stephanie [UNESP] Putarov, Thaila Cristina [UNESP] Tiemi, Caroline [UNESP] Volpe, Lara Mantovani [UNESP] de Oliveira, Carlos Alberto Ferreira de Abreu Glória, Maria Beatriz Carciofi, Aulus Cavalieri [UNESP] |
author_role |
author |
author2 |
Putarov, Thaila Cristina [UNESP] Tiemi, Caroline [UNESP] Volpe, Lara Mantovani [UNESP] de Oliveira, Carlos Alberto Ferreira de Abreu Glória, Maria Beatriz Carciofi, Aulus Cavalieri [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Biorigin Brasil Universidade Federal de Minas Gerais (UFMG) |
dc.contributor.author.fl_str_mv |
de Souza Theodoro, Stephanie [UNESP] Putarov, Thaila Cristina [UNESP] Tiemi, Caroline [UNESP] Volpe, Lara Mantovani [UNESP] de Oliveira, Carlos Alberto Ferreira de Abreu Glória, Maria Beatriz Carciofi, Aulus Cavalieri [UNESP] |
description |
Derivatives of yeast cell wall (YCW) have been studied for their potential prebiotic effects. Recently, new purified and soluble preparations have been developed in an attempt to increase their biological actions. Two YCW preparations, one conventional and another with higher solubility of the mannan oligosaccharide fraction, were evaluated on dogs. One food formulation was used, divided into the following treatments: CON–control, without yeast cell wall addition; YCW–addition of 0.3% of a conventional yeas cell wall extract; YCWs–addition of 0.3% of a yeast cell wall extract with high mannan oligosaccharide solubility. Twenty-four beagle dogs were used, eight per food, distributed on a block design. Blocks lasted 32 days, and TNF-a, IL-6, IL-10, ex vivo production of hydrogen peroxide and nitric oxide by peripheral neutrophils and monocytes, phagocytic index, and fecal IgA were evaluated at the beginning and end of each period. Additionally, nutrient digestibility, feces production and quality, and fermentation products were quantified. The results were evaluated by analysis of variance and compared using the Tukey test (P<0.05), using the basal immunological parameters as a covariate. The inclusion of YCWs reduced fat digestibility (P<0.05), increased the concentration of butyrate and putrescine, and reduced lactate in feces (P<0.05), showing that mannan oligosaccharide solubilization resulted in higher fermentation of this compound and altered the metabolism of the gut microbiota. Lower IL-6 on serum was verified for dogs fed the YCWs diet (P<0.05), suggesting a reduction in the inflammatory activity of dogs. Higher phagocytic index was verified for peripheral monocytes after the intake of the YCW food, suggesting better innate immunity. In conclusion, the solubilization of the mannooligosaccharide fraction alters its interaction with gut microbiota and biological actions in animals, although both yeast cell wall preparations exhibited prebiotic effects on dogs. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-01 2020-12-12T02:30:19Z 2020-12-12T02:30:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0225659 PLoS ONE, v. 14, n. 11, 2019. 1932-6203 http://hdl.handle.net/11449/201349 10.1371/journal.pone.0225659 2-s2.0-85075506535 |
url |
http://dx.doi.org/10.1371/journal.pone.0225659 http://hdl.handle.net/11449/201349 |
identifier_str_mv |
PLoS ONE, v. 14, n. 11, 2019. 1932-6203 10.1371/journal.pone.0225659 2-s2.0-85075506535 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129166554431488 |