Structural and biophysical characterization of the major proteins from the seminal plasma of Dorper rams
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2021.02.019 http://hdl.handle.net/11449/207390 |
Resumo: | Pregnancy rates using frozen semen from rams are higher than for horses. One of the factors that positively influences this effect is the composition of low-molecular-weight proteins from seminal plasma, since the amounts of these proteins are much lower in horses. The aim of this work was to purify the major protein components from ram seminal plasma for structural and biophysical characterization. First, the ram semen was collected and the plasma separated by centrifugation. The protein fractions were isolated by gel filtration chromatography, analyzed by circular dichroism spectroscopy and the amino acid sequence identified by mass spectrometry (LC-MSE), the results of which were used to model the protein structure by bioinformatics techniques. This protein was identified by LC-MSE as a spermadhesin, being an unglycosylated monomer with Tm = 69.3 °C and ΔHm = 371 kJ mol−1 at pH 7.0. This work describes for the first time the structural characterization of a spermadhesin from seminal plasma of Dorper rams. |
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Structural and biophysical characterization of the major proteins from the seminal plasma of Dorper ramsAnimal reproductionProteinSheepSpermadhesinPregnancy rates using frozen semen from rams are higher than for horses. One of the factors that positively influences this effect is the composition of low-molecular-weight proteins from seminal plasma, since the amounts of these proteins are much lower in horses. The aim of this work was to purify the major protein components from ram seminal plasma for structural and biophysical characterization. First, the ram semen was collected and the plasma separated by centrifugation. The protein fractions were isolated by gel filtration chromatography, analyzed by circular dichroism spectroscopy and the amino acid sequence identified by mass spectrometry (LC-MSE), the results of which were used to model the protein structure by bioinformatics techniques. This protein was identified by LC-MSE as a spermadhesin, being an unglycosylated monomer with Tm = 69.3 °C and ΔHm = 371 kJ mol−1 at pH 7.0. This work describes for the first time the structural characterization of a spermadhesin from seminal plasma of Dorper rams.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)College of Dentistry, University of KentuckyConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação AraucáriaDepartment of Veterinary Medicine Universidade Estadual de Maringá – UEMDepartment of Technology Universidade Estadual de Maringá – UEMDepartment of Clinical Analyses and Biomedicine Universidade Estadual de Maringá – UEMDepartment of Chemistry Universidade Estadual de Maringá – UEMDepartment of Physics and Biomolecular Innovation Center (CMIB) Instituto de Biociências Letras e Ciências Exatas – IBILCE/UNESPNational Center for Nuclear Magnetic Resonance of Macromolecules Institute of Medical Biochemistry and National Center for Structural Biology and Bioimaging (CENABIO) UFRJDepartment of Physics and Biomolecular Innovation Center (CMIB) Instituto de Biociências Letras e Ciências Exatas – IBILCE/UNESPFundação Araucária: 40/16Fundação Araucária: 53/19Universidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Universidade Federal do Rio de Janeiro (UFRJ)da Silva, Luan SitóRuivo, Maycon Araújoda Cruz, Tainá MichelleMeneguello, Jean EduardoPilau, Eduardo JorgeCaruso, Ícaro Putinhon [UNESP]de Moraes, Gentil VaniniMartinez, Antônio CampanhaSeixas, Flavio Augusto Vicente2021-06-25T10:54:23Z2021-06-25T10:54:23Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article64-70http://dx.doi.org/10.1016/j.theriogenology.2021.02.019Theriogenology, v. 166, p. 64-70.0093-691Xhttp://hdl.handle.net/11449/20739010.1016/j.theriogenology.2021.02.0192-s2.0-85101986005Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2021-10-23T17:04:30Zoai:repositorio.unesp.br:11449/207390Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:04:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Structural and biophysical characterization of the major proteins from the seminal plasma of Dorper rams |
title |
Structural and biophysical characterization of the major proteins from the seminal plasma of Dorper rams |
spellingShingle |
Structural and biophysical characterization of the major proteins from the seminal plasma of Dorper rams da Silva, Luan Sitó Animal reproduction Protein Sheep Spermadhesin |
title_short |
Structural and biophysical characterization of the major proteins from the seminal plasma of Dorper rams |
title_full |
Structural and biophysical characterization of the major proteins from the seminal plasma of Dorper rams |
title_fullStr |
Structural and biophysical characterization of the major proteins from the seminal plasma of Dorper rams |
title_full_unstemmed |
Structural and biophysical characterization of the major proteins from the seminal plasma of Dorper rams |
title_sort |
Structural and biophysical characterization of the major proteins from the seminal plasma of Dorper rams |
author |
da Silva, Luan Sitó |
author_facet |
da Silva, Luan Sitó Ruivo, Maycon Araújo da Cruz, Tainá Michelle Meneguello, Jean Eduardo Pilau, Eduardo Jorge Caruso, Ícaro Putinhon [UNESP] de Moraes, Gentil Vanini Martinez, Antônio Campanha Seixas, Flavio Augusto Vicente |
author_role |
author |
author2 |
Ruivo, Maycon Araújo da Cruz, Tainá Michelle Meneguello, Jean Eduardo Pilau, Eduardo Jorge Caruso, Ícaro Putinhon [UNESP] de Moraes, Gentil Vanini Martinez, Antônio Campanha Seixas, Flavio Augusto Vicente |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Maringá (UEM) Universidade Estadual Paulista (Unesp) Universidade Federal do Rio de Janeiro (UFRJ) |
dc.contributor.author.fl_str_mv |
da Silva, Luan Sitó Ruivo, Maycon Araújo da Cruz, Tainá Michelle Meneguello, Jean Eduardo Pilau, Eduardo Jorge Caruso, Ícaro Putinhon [UNESP] de Moraes, Gentil Vanini Martinez, Antônio Campanha Seixas, Flavio Augusto Vicente |
dc.subject.por.fl_str_mv |
Animal reproduction Protein Sheep Spermadhesin |
topic |
Animal reproduction Protein Sheep Spermadhesin |
description |
Pregnancy rates using frozen semen from rams are higher than for horses. One of the factors that positively influences this effect is the composition of low-molecular-weight proteins from seminal plasma, since the amounts of these proteins are much lower in horses. The aim of this work was to purify the major protein components from ram seminal plasma for structural and biophysical characterization. First, the ram semen was collected and the plasma separated by centrifugation. The protein fractions were isolated by gel filtration chromatography, analyzed by circular dichroism spectroscopy and the amino acid sequence identified by mass spectrometry (LC-MSE), the results of which were used to model the protein structure by bioinformatics techniques. This protein was identified by LC-MSE as a spermadhesin, being an unglycosylated monomer with Tm = 69.3 °C and ΔHm = 371 kJ mol−1 at pH 7.0. This work describes for the first time the structural characterization of a spermadhesin from seminal plasma of Dorper rams. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:54:23Z 2021-06-25T10:54:23Z 2021-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2021.02.019 Theriogenology, v. 166, p. 64-70. 0093-691X http://hdl.handle.net/11449/207390 10.1016/j.theriogenology.2021.02.019 2-s2.0-85101986005 |
url |
http://dx.doi.org/10.1016/j.theriogenology.2021.02.019 http://hdl.handle.net/11449/207390 |
identifier_str_mv |
Theriogenology, v. 166, p. 64-70. 0093-691X 10.1016/j.theriogenology.2021.02.019 2-s2.0-85101986005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
64-70 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046459286749184 |