Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks

Detalhes bibliográficos
Autor(a) principal: Joaquim, S. F. [UNESP]
Data de Publicação: 2018
Outros Autores: Guimaraes, F. F. [UNESP], Salina, A. [UNESP], Junqueira, N. B. [UNESP], Gomes, E. N. [UNESP], Langoni, H. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1678-4162-9982
http://hdl.handle.net/11449/186331
Resumo: Mastitis caused by Mycoplasma spp., regardless of species, are considered highly contagious pathogens and, usually was not responsive to antimicrobial therapy. Five dairy herds, comprising 489 animals and 1,956 mammary glands, were used in this study. Milk samples were obtained from bulk tanks and subjected to polymerase chain reaction (PCR) for the identification of Mollicutes, Mycoplasma spp., and Mycoplasma bovis. Moreover, individual samples from cases of clinical and subclinical mastitis in quarters of the dairy herds' animals that yielded a positive PCR upon bulk tank analysis were subjected to molecular analysis. Only one bulk tank was positive for class Mollicutes by PCR. All positive samples classified as mastitis teats had their DNA extracted and tested by PCR for both class Mollicutes and M. bovis. Of these, two (2.08%) were positive for Mycoplasma genus, although none was positive for M. bovis. This result suggests that the PCR of bulk tanks is a viable tool in monitoring and preventing mastitis infections caused by Mycoplasma spp.
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spelling Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanksmilk qualitypolymerase chain reactiondairy herdsMastitis caused by Mycoplasma spp., regardless of species, are considered highly contagious pathogens and, usually was not responsive to antimicrobial therapy. Five dairy herds, comprising 489 animals and 1,956 mammary glands, were used in this study. Milk samples were obtained from bulk tanks and subjected to polymerase chain reaction (PCR) for the identification of Mollicutes, Mycoplasma spp., and Mycoplasma bovis. Moreover, individual samples from cases of clinical and subclinical mastitis in quarters of the dairy herds' animals that yielded a positive PCR upon bulk tank analysis were subjected to molecular analysis. Only one bulk tank was positive for class Mollicutes by PCR. All positive samples classified as mastitis teats had their DNA extracted and tested by PCR for both class Mollicutes and M. bovis. Of these, two (2.08%) were positive for Mycoplasma genus, although none was positive for M. bovis. This result suggests that the PCR of bulk tanks is a viable tool in monitoring and preventing mastitis infections caused by Mycoplasma spp.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu, SP, BrazilFAPESP: 2011/14135-0Arquivo Brasileiro Medicina Veterinaria ZootecniaUniversidade Estadual Paulista (Unesp)Joaquim, S. F. [UNESP]Guimaraes, F. F. [UNESP]Salina, A. [UNESP]Junqueira, N. B. [UNESP]Gomes, E. N. [UNESP]Langoni, H. [UNESP]2019-10-04T18:54:05Z2019-10-04T18:54:05Z2018-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1793-1797application/pdfhttp://dx.doi.org/10.1590/1678-4162-9982Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 70, n. 6, p. 1793-1797, 2018.0102-0935http://hdl.handle.net/11449/18633110.1590/1678-4162-9982S0102-09352018000601793WOS:000452984200018S0102-09352018000601793.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro De Medicina Veterinaria E Zootecniainfo:eu-repo/semantics/openAccess2023-10-06T06:06:28Zoai:repositorio.unesp.br:11449/186331Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:10:00.743345Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks
title Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks
spellingShingle Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks
Joaquim, S. F. [UNESP]
milk quality
polymerase chain reaction
dairy herds
title_short Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks
title_full Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks
title_fullStr Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks
title_full_unstemmed Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks
title_sort Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks
author Joaquim, S. F. [UNESP]
author_facet Joaquim, S. F. [UNESP]
Guimaraes, F. F. [UNESP]
Salina, A. [UNESP]
Junqueira, N. B. [UNESP]
Gomes, E. N. [UNESP]
Langoni, H. [UNESP]
author_role author
author2 Guimaraes, F. F. [UNESP]
Salina, A. [UNESP]
Junqueira, N. B. [UNESP]
Gomes, E. N. [UNESP]
Langoni, H. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Joaquim, S. F. [UNESP]
Guimaraes, F. F. [UNESP]
Salina, A. [UNESP]
Junqueira, N. B. [UNESP]
Gomes, E. N. [UNESP]
Langoni, H. [UNESP]
dc.subject.por.fl_str_mv milk quality
polymerase chain reaction
dairy herds
topic milk quality
polymerase chain reaction
dairy herds
description Mastitis caused by Mycoplasma spp., regardless of species, are considered highly contagious pathogens and, usually was not responsive to antimicrobial therapy. Five dairy herds, comprising 489 animals and 1,956 mammary glands, were used in this study. Milk samples were obtained from bulk tanks and subjected to polymerase chain reaction (PCR) for the identification of Mollicutes, Mycoplasma spp., and Mycoplasma bovis. Moreover, individual samples from cases of clinical and subclinical mastitis in quarters of the dairy herds' animals that yielded a positive PCR upon bulk tank analysis were subjected to molecular analysis. Only one bulk tank was positive for class Mollicutes by PCR. All positive samples classified as mastitis teats had their DNA extracted and tested by PCR for both class Mollicutes and M. bovis. Of these, two (2.08%) were positive for Mycoplasma genus, although none was positive for M. bovis. This result suggests that the PCR of bulk tanks is a viable tool in monitoring and preventing mastitis infections caused by Mycoplasma spp.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-01
2019-10-04T18:54:05Z
2019-10-04T18:54:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-4162-9982
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 70, n. 6, p. 1793-1797, 2018.
0102-0935
http://hdl.handle.net/11449/186331
10.1590/1678-4162-9982
S0102-09352018000601793
WOS:000452984200018
S0102-09352018000601793.pdf
url http://dx.doi.org/10.1590/1678-4162-9982
http://hdl.handle.net/11449/186331
identifier_str_mv Arquivo Brasileiro De Medicina Veterinaria E Zootecnia. Minas Gerais: Arquivo Brasileiro Medicina Veterinaria Zootecnia, v. 70, n. 6, p. 1793-1797, 2018.
0102-0935
10.1590/1678-4162-9982
S0102-09352018000601793
WOS:000452984200018
S0102-09352018000601793.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivo Brasileiro De Medicina Veterinaria E Zootecnia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1793-1797
application/pdf
dc.publisher.none.fl_str_mv Arquivo Brasileiro Medicina Veterinaria Zootecnia
publisher.none.fl_str_mv Arquivo Brasileiro Medicina Veterinaria Zootecnia
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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