Anti-inflammatory and toxicological evaluation of essential oil from Piper glabratum leaves
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Publication Date: | 2017 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.jep.2017.01.008 http://hdl.handle.net/11449/174140 |
Summary: | Ethnopharmacological relevance Although some of the species of the genus Piper exhibit interesting biological properties, studies on Piper glabratum Kunth are very limited. Aim of the study This study investigated the anti-inflammatory activity and the toxicological profile of the essential oil from P. glabratum leaves (OEPG) in mice. Materials and Methods The acute toxicity of OEPG was evaluated by oral administration to female mice as single doses of 500, 1000, 2000 or 5000 mg/kg/body weight. In the subacute toxicity test, the females received 500 or 1000 mg/kg/body weight of OEPG for 28 days. The anti-inflammatory potential of OEPG was evaluated using four models including pleurisy, edema, mechanical hyperalgesia and cold allodynia models in mouse paws. Results No clinical signs of toxicity were observed in animals after acute treatment, which suggested that the LD50 is greater than 5000 mg/kg. The subacute exposure to OEPG produced no significant changes in the hematological or biochemical parameters. Similarly, the histology of the organs and the estrus cycle displayed no marked alterations. OEPG exhibited anti-inflammatory activity as indicated by inhibition of the leukocyte migration (100, 300, 700 mg/kg) and the protein extravasation into the pleural exudates (700 mg/kg). After intraplantar injection of carrageenan, it was observed that the 700 mg/kg dose of OEPG reduced edema formation and decreased the sensitivity to mechanical stimulation and cold. Conclusions These results demonstrate the anti-inflammatory potential of the essential oil of P. glabratum leaves in the absence of toxicity in female mice. |
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Anti-inflammatory and toxicological evaluation of essential oil from Piper glabratum leavesCarrageenanEssential oilInflammationPiper glabratumToxicityEthnopharmacological relevance Although some of the species of the genus Piper exhibit interesting biological properties, studies on Piper glabratum Kunth are very limited. Aim of the study This study investigated the anti-inflammatory activity and the toxicological profile of the essential oil from P. glabratum leaves (OEPG) in mice. Materials and Methods The acute toxicity of OEPG was evaluated by oral administration to female mice as single doses of 500, 1000, 2000 or 5000 mg/kg/body weight. In the subacute toxicity test, the females received 500 or 1000 mg/kg/body weight of OEPG for 28 days. The anti-inflammatory potential of OEPG was evaluated using four models including pleurisy, edema, mechanical hyperalgesia and cold allodynia models in mouse paws. Results No clinical signs of toxicity were observed in animals after acute treatment, which suggested that the LD50 is greater than 5000 mg/kg. The subacute exposure to OEPG produced no significant changes in the hematological or biochemical parameters. Similarly, the histology of the organs and the estrus cycle displayed no marked alterations. OEPG exhibited anti-inflammatory activity as indicated by inhibition of the leukocyte migration (100, 300, 700 mg/kg) and the protein extravasation into the pleural exudates (700 mg/kg). After intraplantar injection of carrageenan, it was observed that the 700 mg/kg dose of OEPG reduced edema formation and decreased the sensitivity to mechanical stimulation and cold. Conclusions These results demonstrate the anti-inflammatory potential of the essential oil of P. glabratum leaves in the absence of toxicity in female mice.School of Health Sciences Federal University of Grande DouradosMato Grosso do Sul State University (UEMS)Department of Morphology Institute of Biosciences of Botucatu UNESP – Univ. Estadual PaulistaDepartment of Morphology Institute of Biosciences of Botucatu UNESP – Univ. Estadual PaulistaFederal University of Grande DouradosUniversidade Estadual de Mato Grosso do Sul (UEMS)Universidade Estadual Paulista (Unesp)Branquinho, Lidiane SchultzSantos, Joyce AlencarCardoso, Claudia Andrea LimaMota, Jonas da SilvaJunior, Ubirajara LanzaKassuya, Cândida Aparecida LeiteArena, Arielle Cristina [UNESP]2018-12-11T17:09:31Z2018-12-11T17:09:31Z2017-02-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article372-378http://dx.doi.org/10.1016/j.jep.2017.01.008Journal of Ethnopharmacology, v. 198, p. 372-378.1872-75730378-8741http://hdl.handle.net/11449/17414010.1016/j.jep.2017.01.0082-s2.0-85010880298Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Ethnopharmacology1,150info:eu-repo/semantics/openAccess2021-10-23T16:16:18Zoai:repositorio.unesp.br:11449/174140Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T16:16:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anti-inflammatory and toxicological evaluation of essential oil from Piper glabratum leaves |
title |
Anti-inflammatory and toxicological evaluation of essential oil from Piper glabratum leaves |
spellingShingle |
Anti-inflammatory and toxicological evaluation of essential oil from Piper glabratum leaves Branquinho, Lidiane Schultz Carrageenan Essential oil Inflammation Piper glabratum Toxicity |
title_short |
Anti-inflammatory and toxicological evaluation of essential oil from Piper glabratum leaves |
title_full |
Anti-inflammatory and toxicological evaluation of essential oil from Piper glabratum leaves |
title_fullStr |
Anti-inflammatory and toxicological evaluation of essential oil from Piper glabratum leaves |
title_full_unstemmed |
Anti-inflammatory and toxicological evaluation of essential oil from Piper glabratum leaves |
title_sort |
Anti-inflammatory and toxicological evaluation of essential oil from Piper glabratum leaves |
author |
Branquinho, Lidiane Schultz |
author_facet |
Branquinho, Lidiane Schultz Santos, Joyce Alencar Cardoso, Claudia Andrea Lima Mota, Jonas da Silva Junior, Ubirajara Lanza Kassuya, Cândida Aparecida Leite Arena, Arielle Cristina [UNESP] |
author_role |
author |
author2 |
Santos, Joyce Alencar Cardoso, Claudia Andrea Lima Mota, Jonas da Silva Junior, Ubirajara Lanza Kassuya, Cândida Aparecida Leite Arena, Arielle Cristina [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Grande Dourados Universidade Estadual de Mato Grosso do Sul (UEMS) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Branquinho, Lidiane Schultz Santos, Joyce Alencar Cardoso, Claudia Andrea Lima Mota, Jonas da Silva Junior, Ubirajara Lanza Kassuya, Cândida Aparecida Leite Arena, Arielle Cristina [UNESP] |
dc.subject.por.fl_str_mv |
Carrageenan Essential oil Inflammation Piper glabratum Toxicity |
topic |
Carrageenan Essential oil Inflammation Piper glabratum Toxicity |
description |
Ethnopharmacological relevance Although some of the species of the genus Piper exhibit interesting biological properties, studies on Piper glabratum Kunth are very limited. Aim of the study This study investigated the anti-inflammatory activity and the toxicological profile of the essential oil from P. glabratum leaves (OEPG) in mice. Materials and Methods The acute toxicity of OEPG was evaluated by oral administration to female mice as single doses of 500, 1000, 2000 or 5000 mg/kg/body weight. In the subacute toxicity test, the females received 500 or 1000 mg/kg/body weight of OEPG for 28 days. The anti-inflammatory potential of OEPG was evaluated using four models including pleurisy, edema, mechanical hyperalgesia and cold allodynia models in mouse paws. Results No clinical signs of toxicity were observed in animals after acute treatment, which suggested that the LD50 is greater than 5000 mg/kg. The subacute exposure to OEPG produced no significant changes in the hematological or biochemical parameters. Similarly, the histology of the organs and the estrus cycle displayed no marked alterations. OEPG exhibited anti-inflammatory activity as indicated by inhibition of the leukocyte migration (100, 300, 700 mg/kg) and the protein extravasation into the pleural exudates (700 mg/kg). After intraplantar injection of carrageenan, it was observed that the 700 mg/kg dose of OEPG reduced edema formation and decreased the sensitivity to mechanical stimulation and cold. Conclusions These results demonstrate the anti-inflammatory potential of the essential oil of P. glabratum leaves in the absence of toxicity in female mice. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-23 2018-12-11T17:09:31Z 2018-12-11T17:09:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jep.2017.01.008 Journal of Ethnopharmacology, v. 198, p. 372-378. 1872-7573 0378-8741 http://hdl.handle.net/11449/174140 10.1016/j.jep.2017.01.008 2-s2.0-85010880298 |
url |
http://dx.doi.org/10.1016/j.jep.2017.01.008 http://hdl.handle.net/11449/174140 |
identifier_str_mv |
Journal of Ethnopharmacology, v. 198, p. 372-378. 1872-7573 0378-8741 10.1016/j.jep.2017.01.008 2-s2.0-85010880298 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Ethnopharmacology 1,150 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
372-378 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965239008034816 |