Emodin, Physcion, and Crude Extract of Rhamnus sphaerosperma var. pubescens Induce Mixed Cell Death, Increase in Oxidative Stress, DNA Damage, and Inhibition of AKT in Cervical and Oral Squamous Carcinoma Cell Lines
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2018/2390234 http://hdl.handle.net/11449/164432 |
Resumo: | There have been few studies on the pharmacological properties of Rhamnus sphaerosperma var. pubescens, a native Brazilian species popularly known as fruto-de-pombo. The aim of this study was to investigate the scavenging capacity of emodin, physcion, and the ethanolic crude extract of Rhamnus sphaerosperma var. pubescens against reactive oxygen and nitrogen species, as well as their role and plausible mechanisms in prompting cell death and changes in AKT phosphorylation after cervical (SiHa and C33A) and oral (HSC-3) squamous cell carcinoma treatments. Emodin was shown to be the best scavenger of NO center dot and O-2(center dot-), while all samples were equally effective in HOCl/OCl- capture. Emodin, physcion, and the ethanolic extract all exhibited cytotoxic effects on SiHa, C33A, HSC3, and HaCaT (immortalized human keratinocytes, nontumorigenic cell line), involving mixed cell death (apoptosis and necrosis) independent of the caspase activation pathway. Emodin, physcion, and the ethanolic extract increased intracellular oxidative stress and DNA damage. Emodin decreased the activation of AKT in all tumor cells, physcion in HSC3 and HaCaT cells, and the ethanolic extract in C33A and HaCaT cells, respectively. The induction of cancer cell death by emodin, physcion, and the ethanolic crude extract of Rhamnus sphaerosperma var. pubescens was related to an increase in intracellular oxidative stress and DNA damage and a decrease in AKT activation. These molecules are therefore emerging as interesting candidates for further study as novel options to treat cervical and oral carcinomas. |
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Emodin, Physcion, and Crude Extract of Rhamnus sphaerosperma var. pubescens Induce Mixed Cell Death, Increase in Oxidative Stress, DNA Damage, and Inhibition of AKT in Cervical and Oral Squamous Carcinoma Cell LinesThere have been few studies on the pharmacological properties of Rhamnus sphaerosperma var. pubescens, a native Brazilian species popularly known as fruto-de-pombo. The aim of this study was to investigate the scavenging capacity of emodin, physcion, and the ethanolic crude extract of Rhamnus sphaerosperma var. pubescens against reactive oxygen and nitrogen species, as well as their role and plausible mechanisms in prompting cell death and changes in AKT phosphorylation after cervical (SiHa and C33A) and oral (HSC-3) squamous cell carcinoma treatments. Emodin was shown to be the best scavenger of NO center dot and O-2(center dot-), while all samples were equally effective in HOCl/OCl- capture. Emodin, physcion, and the ethanolic extract all exhibited cytotoxic effects on SiHa, C33A, HSC3, and HaCaT (immortalized human keratinocytes, nontumorigenic cell line), involving mixed cell death (apoptosis and necrosis) independent of the caspase activation pathway. Emodin, physcion, and the ethanolic extract increased intracellular oxidative stress and DNA damage. Emodin decreased the activation of AKT in all tumor cells, physcion in HSC3 and HaCaT cells, and the ethanolic extract in C33A and HaCaT cells, respectively. The induction of cancer cell death by emodin, physcion, and the ethanolic crude extract of Rhamnus sphaerosperma var. pubescens was related to an increase in intracellular oxidative stress and DNA damage and a decrease in AKT activation. These molecules are therefore emerging as interesting candidates for further study as novel options to treat cervical and oral carcinomas.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Scientific Support and Development Program of the School of Pharmaceutical Sciences of UNESP (PADC/FCFAr, UNESP)Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Clin Anal, BR-14800903 Araraquara, SP, BrazilFed Univ Parana UFPR, Dept Pharm, BR-80210170 Curitiba, PR, BrazilFed Univ Parana UFPR, Dept Chem, BR-80210170 Curitiba, PR, BrazilSao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Clin Anal, BR-14800903 Araraquara, SP, BrazilHindawi LtdUniversidade Estadual Paulista (Unesp)Universidade Federal do Paraná (UFPR)Moreira, Thais Fernanda [UNESP]Sorbo, Juliana Maria [UNESP]Souza, Felipe de Oliveira [UNESP]Fernandes, Barbara Colatto [UNESP]Marins Ocampos, Fernanda MariaSoares de Oliveira, Daniella MariaArcaro, Carlos Alberto [UNESP]Assis, Renata Pires [UNESP]Barison, AnderssonMiguel, Obdulio GomesBaviera, Amanda Martins [UNESP]Soares, Christiane Pienna [UNESP]Brunetti, Iguatemy Lourenco [UNESP]2018-11-26T17:54:32Z2018-11-26T17:54:32Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article18application/pdfhttp://dx.doi.org/10.1155/2018/2390234Oxidative Medicine And Cellular Longevity. London: Hindawi Ltd, 18 p., 2018.1942-0900http://hdl.handle.net/11449/16443210.1155/2018/2390234WOS:000438753000001WOS000438753000001.pdf17680252903736690000-0003-1740-7360Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOxidative Medicine And Cellular Longevity1,558info:eu-repo/semantics/openAccess2024-06-21T15:19:09Zoai:repositorio.unesp.br:11449/164432Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:21:45.791617Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Emodin, Physcion, and Crude Extract of Rhamnus sphaerosperma var. pubescens Induce Mixed Cell Death, Increase in Oxidative Stress, DNA Damage, and Inhibition of AKT in Cervical and Oral Squamous Carcinoma Cell Lines |
title |
Emodin, Physcion, and Crude Extract of Rhamnus sphaerosperma var. pubescens Induce Mixed Cell Death, Increase in Oxidative Stress, DNA Damage, and Inhibition of AKT in Cervical and Oral Squamous Carcinoma Cell Lines |
spellingShingle |
Emodin, Physcion, and Crude Extract of Rhamnus sphaerosperma var. pubescens Induce Mixed Cell Death, Increase in Oxidative Stress, DNA Damage, and Inhibition of AKT in Cervical and Oral Squamous Carcinoma Cell Lines Moreira, Thais Fernanda [UNESP] |
title_short |
Emodin, Physcion, and Crude Extract of Rhamnus sphaerosperma var. pubescens Induce Mixed Cell Death, Increase in Oxidative Stress, DNA Damage, and Inhibition of AKT in Cervical and Oral Squamous Carcinoma Cell Lines |
title_full |
Emodin, Physcion, and Crude Extract of Rhamnus sphaerosperma var. pubescens Induce Mixed Cell Death, Increase in Oxidative Stress, DNA Damage, and Inhibition of AKT in Cervical and Oral Squamous Carcinoma Cell Lines |
title_fullStr |
Emodin, Physcion, and Crude Extract of Rhamnus sphaerosperma var. pubescens Induce Mixed Cell Death, Increase in Oxidative Stress, DNA Damage, and Inhibition of AKT in Cervical and Oral Squamous Carcinoma Cell Lines |
title_full_unstemmed |
Emodin, Physcion, and Crude Extract of Rhamnus sphaerosperma var. pubescens Induce Mixed Cell Death, Increase in Oxidative Stress, DNA Damage, and Inhibition of AKT in Cervical and Oral Squamous Carcinoma Cell Lines |
title_sort |
Emodin, Physcion, and Crude Extract of Rhamnus sphaerosperma var. pubescens Induce Mixed Cell Death, Increase in Oxidative Stress, DNA Damage, and Inhibition of AKT in Cervical and Oral Squamous Carcinoma Cell Lines |
author |
Moreira, Thais Fernanda [UNESP] |
author_facet |
Moreira, Thais Fernanda [UNESP] Sorbo, Juliana Maria [UNESP] Souza, Felipe de Oliveira [UNESP] Fernandes, Barbara Colatto [UNESP] Marins Ocampos, Fernanda Maria Soares de Oliveira, Daniella Maria Arcaro, Carlos Alberto [UNESP] Assis, Renata Pires [UNESP] Barison, Andersson Miguel, Obdulio Gomes Baviera, Amanda Martins [UNESP] Soares, Christiane Pienna [UNESP] Brunetti, Iguatemy Lourenco [UNESP] |
author_role |
author |
author2 |
Sorbo, Juliana Maria [UNESP] Souza, Felipe de Oliveira [UNESP] Fernandes, Barbara Colatto [UNESP] Marins Ocampos, Fernanda Maria Soares de Oliveira, Daniella Maria Arcaro, Carlos Alberto [UNESP] Assis, Renata Pires [UNESP] Barison, Andersson Miguel, Obdulio Gomes Baviera, Amanda Martins [UNESP] Soares, Christiane Pienna [UNESP] Brunetti, Iguatemy Lourenco [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal do Paraná (UFPR) |
dc.contributor.author.fl_str_mv |
Moreira, Thais Fernanda [UNESP] Sorbo, Juliana Maria [UNESP] Souza, Felipe de Oliveira [UNESP] Fernandes, Barbara Colatto [UNESP] Marins Ocampos, Fernanda Maria Soares de Oliveira, Daniella Maria Arcaro, Carlos Alberto [UNESP] Assis, Renata Pires [UNESP] Barison, Andersson Miguel, Obdulio Gomes Baviera, Amanda Martins [UNESP] Soares, Christiane Pienna [UNESP] Brunetti, Iguatemy Lourenco [UNESP] |
description |
There have been few studies on the pharmacological properties of Rhamnus sphaerosperma var. pubescens, a native Brazilian species popularly known as fruto-de-pombo. The aim of this study was to investigate the scavenging capacity of emodin, physcion, and the ethanolic crude extract of Rhamnus sphaerosperma var. pubescens against reactive oxygen and nitrogen species, as well as their role and plausible mechanisms in prompting cell death and changes in AKT phosphorylation after cervical (SiHa and C33A) and oral (HSC-3) squamous cell carcinoma treatments. Emodin was shown to be the best scavenger of NO center dot and O-2(center dot-), while all samples were equally effective in HOCl/OCl- capture. Emodin, physcion, and the ethanolic extract all exhibited cytotoxic effects on SiHa, C33A, HSC3, and HaCaT (immortalized human keratinocytes, nontumorigenic cell line), involving mixed cell death (apoptosis and necrosis) independent of the caspase activation pathway. Emodin, physcion, and the ethanolic extract increased intracellular oxidative stress and DNA damage. Emodin decreased the activation of AKT in all tumor cells, physcion in HSC3 and HaCaT cells, and the ethanolic extract in C33A and HaCaT cells, respectively. The induction of cancer cell death by emodin, physcion, and the ethanolic crude extract of Rhamnus sphaerosperma var. pubescens was related to an increase in intracellular oxidative stress and DNA damage and a decrease in AKT activation. These molecules are therefore emerging as interesting candidates for further study as novel options to treat cervical and oral carcinomas. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:54:32Z 2018-11-26T17:54:32Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2018/2390234 Oxidative Medicine And Cellular Longevity. London: Hindawi Ltd, 18 p., 2018. 1942-0900 http://hdl.handle.net/11449/164432 10.1155/2018/2390234 WOS:000438753000001 WOS000438753000001.pdf 1768025290373669 0000-0003-1740-7360 |
url |
http://dx.doi.org/10.1155/2018/2390234 http://hdl.handle.net/11449/164432 |
identifier_str_mv |
Oxidative Medicine And Cellular Longevity. London: Hindawi Ltd, 18 p., 2018. 1942-0900 10.1155/2018/2390234 WOS:000438753000001 WOS000438753000001.pdf 1768025290373669 0000-0003-1740-7360 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oxidative Medicine And Cellular Longevity 1,558 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
18 application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Ltd |
publisher.none.fl_str_mv |
Hindawi Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129192426995712 |