Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)

Detalhes bibliográficos
Autor(a) principal: Tanan, C. L.
Data de Publicação: 2006
Outros Autores: Ventura, D. F., de Souza, J. M., Grotzner, S. R., Mela, M., Gouveia, A. [UNESP], Oliveira-Ribeiro, C. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2006000700017
http://hdl.handle.net/11449/8621
Resumo: Methyl mercury (MeHg) is highly neurotoxic, affecting visual function in addition to other central nervous system functions. The effect of mercury intoxication on the amplitude of horizontal cell responses to light was studied in the retina of the fish Hoplias malabaricus. Intracellular responses were recorded from horizontal cells of fish previously intoxicated with MeHg by intraperitoneal injection (IP group) or by trophic exposure (T group). Only one retina per fish was used. The doses of MeHg chloride administered to the IP group were 0.01, 0.05, 0.1, 1.0, 2.0, and 6.0 mg/kg. The amplitudes of the horizontal cell responses were lower than control in individuals exposed to 0.01 (N = 4 retinas), 0.05 (N = 2 retinas) and 0.1 mg/kg (N = 1 retina), whereas no responses were recorded in the 1.0, 2.0, and 6.0 mg/kg groups. T group individuals were fed young specimens of Astyanax sp previously injected with MeHg corresponding to 0.75 (N = 1 retina), 0.075 (N = 8 retinas) or 0.0075 (N = 4 retinas) mg/kg fish body weight. After 14 doses, one every 5 days, the amplitude of the horizontal cell response was higher than control in individuals exposed to 0.075 and 0.0075 mg/kg, and lower in individuals exposed to 0.75 mg/kg. We conclude that intoxication with MeHg affects the electrophysiological response of the horizontal cells in the retina, either reducing or increasing its amplitude compared to control, and that these effects are related to the dose and/or to the mode of administration.
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spelling Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)methyl mercuryhorizontal cellselectrophysiologyHoplias malabaricusretinaMethyl mercury (MeHg) is highly neurotoxic, affecting visual function in addition to other central nervous system functions. The effect of mercury intoxication on the amplitude of horizontal cell responses to light was studied in the retina of the fish Hoplias malabaricus. Intracellular responses were recorded from horizontal cells of fish previously intoxicated with MeHg by intraperitoneal injection (IP group) or by trophic exposure (T group). Only one retina per fish was used. The doses of MeHg chloride administered to the IP group were 0.01, 0.05, 0.1, 1.0, 2.0, and 6.0 mg/kg. The amplitudes of the horizontal cell responses were lower than control in individuals exposed to 0.01 (N = 4 retinas), 0.05 (N = 2 retinas) and 0.1 mg/kg (N = 1 retina), whereas no responses were recorded in the 1.0, 2.0, and 6.0 mg/kg groups. T group individuals were fed young specimens of Astyanax sp previously injected with MeHg corresponding to 0.75 (N = 1 retina), 0.075 (N = 8 retinas) or 0.0075 (N = 4 retinas) mg/kg fish body weight. After 14 doses, one every 5 days, the amplitude of the horizontal cell response was higher than control in individuals exposed to 0.075 and 0.0075 mg/kg, and lower in individuals exposed to 0.75 mg/kg. We conclude that intoxication with MeHg affects the electrophysiological response of the horizontal cells in the retina, either reducing or increasing its amplitude compared to control, and that these effects are related to the dose and/or to the mode of administration.Univ São Paulo, Inst Psicol, Dept Psicol Expt, Ctr Neurociencias & Comportamento, BR-05508900 São Paulo, BrazilUniversidade Federal do Paraná (UFPF), Dept Biol Celular, BR-80060000 Curitiba, Parana, BrazilUNESP, Dept Psicol, Univ Estadual Paulista Julio de Mesquita Filho, Bauru, SP, BrazilUNESP, Dept Psicol, Univ Estadual Paulista Julio de Mesquita Filho, Bauru, SP, BrazilAssociação Brasileira de Divulgação Científica (ABRADIC)Universidade de São Paulo (USP)Universidade Federal do Paraná (UFPR)Universidade Estadual Paulista (Unesp)Tanan, C. L.Ventura, D. F.de Souza, J. M.Grotzner, S. R.Mela, M.Gouveia, A. [UNESP]Oliveira-Ribeiro, C. A.2014-05-20T13:26:38Z2014-05-20T13:26:38Z2006-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article987-995application/pdfhttp://dx.doi.org/10.1590/S0100-879X2006000700017Brazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 39, n. 7, p. 987-995, 2006.0100-879Xhttp://hdl.handle.net/11449/862110.1590/S0100-879X2006000700017S0100-879X2006000700017WOS:0002396242000172-s2.0-33746345629WOS000239624200017.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Research1.492info:eu-repo/semantics/openAccess2024-04-29T15:53:53Zoai:repositorio.unesp.br:11449/8621Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:27:05.313974Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)
title Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)
spellingShingle Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)
Tanan, C. L.
methyl mercury
horizontal cells
electrophysiology
Hoplias malabaricus
retina
title_short Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)
title_full Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)
title_fullStr Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)
title_full_unstemmed Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)
title_sort Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)
author Tanan, C. L.
author_facet Tanan, C. L.
Ventura, D. F.
de Souza, J. M.
Grotzner, S. R.
Mela, M.
Gouveia, A. [UNESP]
Oliveira-Ribeiro, C. A.
author_role author
author2 Ventura, D. F.
de Souza, J. M.
Grotzner, S. R.
Mela, M.
Gouveia, A. [UNESP]
Oliveira-Ribeiro, C. A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Federal do Paraná (UFPR)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Tanan, C. L.
Ventura, D. F.
de Souza, J. M.
Grotzner, S. R.
Mela, M.
Gouveia, A. [UNESP]
Oliveira-Ribeiro, C. A.
dc.subject.por.fl_str_mv methyl mercury
horizontal cells
electrophysiology
Hoplias malabaricus
retina
topic methyl mercury
horizontal cells
electrophysiology
Hoplias malabaricus
retina
description Methyl mercury (MeHg) is highly neurotoxic, affecting visual function in addition to other central nervous system functions. The effect of mercury intoxication on the amplitude of horizontal cell responses to light was studied in the retina of the fish Hoplias malabaricus. Intracellular responses were recorded from horizontal cells of fish previously intoxicated with MeHg by intraperitoneal injection (IP group) or by trophic exposure (T group). Only one retina per fish was used. The doses of MeHg chloride administered to the IP group were 0.01, 0.05, 0.1, 1.0, 2.0, and 6.0 mg/kg. The amplitudes of the horizontal cell responses were lower than control in individuals exposed to 0.01 (N = 4 retinas), 0.05 (N = 2 retinas) and 0.1 mg/kg (N = 1 retina), whereas no responses were recorded in the 1.0, 2.0, and 6.0 mg/kg groups. T group individuals were fed young specimens of Astyanax sp previously injected with MeHg corresponding to 0.75 (N = 1 retina), 0.075 (N = 8 retinas) or 0.0075 (N = 4 retinas) mg/kg fish body weight. After 14 doses, one every 5 days, the amplitude of the horizontal cell response was higher than control in individuals exposed to 0.075 and 0.0075 mg/kg, and lower in individuals exposed to 0.75 mg/kg. We conclude that intoxication with MeHg affects the electrophysiological response of the horizontal cells in the retina, either reducing or increasing its amplitude compared to control, and that these effects are related to the dose and/or to the mode of administration.
publishDate 2006
dc.date.none.fl_str_mv 2006-07-01
2014-05-20T13:26:38Z
2014-05-20T13:26:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2006000700017
Brazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 39, n. 7, p. 987-995, 2006.
0100-879X
http://hdl.handle.net/11449/8621
10.1590/S0100-879X2006000700017
S0100-879X2006000700017
WOS:000239624200017
2-s2.0-33746345629
WOS000239624200017.pdf
url http://dx.doi.org/10.1590/S0100-879X2006000700017
http://hdl.handle.net/11449/8621
identifier_str_mv Brazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 39, n. 7, p. 987-995, 2006.
0100-879X
10.1590/S0100-879X2006000700017
S0100-879X2006000700017
WOS:000239624200017
2-s2.0-33746345629
WOS000239624200017.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
1.492
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 987-995
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica (ABRADIC)
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica (ABRADIC)
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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