Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-879X2006000700017 http://hdl.handle.net/11449/8621 |
Resumo: | Methyl mercury (MeHg) is highly neurotoxic, affecting visual function in addition to other central nervous system functions. The effect of mercury intoxication on the amplitude of horizontal cell responses to light was studied in the retina of the fish Hoplias malabaricus. Intracellular responses were recorded from horizontal cells of fish previously intoxicated with MeHg by intraperitoneal injection (IP group) or by trophic exposure (T group). Only one retina per fish was used. The doses of MeHg chloride administered to the IP group were 0.01, 0.05, 0.1, 1.0, 2.0, and 6.0 mg/kg. The amplitudes of the horizontal cell responses were lower than control in individuals exposed to 0.01 (N = 4 retinas), 0.05 (N = 2 retinas) and 0.1 mg/kg (N = 1 retina), whereas no responses were recorded in the 1.0, 2.0, and 6.0 mg/kg groups. T group individuals were fed young specimens of Astyanax sp previously injected with MeHg corresponding to 0.75 (N = 1 retina), 0.075 (N = 8 retinas) or 0.0075 (N = 4 retinas) mg/kg fish body weight. After 14 doses, one every 5 days, the amplitude of the horizontal cell response was higher than control in individuals exposed to 0.075 and 0.0075 mg/kg, and lower in individuals exposed to 0.75 mg/kg. We conclude that intoxication with MeHg affects the electrophysiological response of the horizontal cells in the retina, either reducing or increasing its amplitude compared to control, and that these effects are related to the dose and/or to the mode of administration. |
id |
UNSP_3b111bbcab55511799048e60fa8c66b3 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/8621 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus)methyl mercuryhorizontal cellselectrophysiologyHoplias malabaricusretinaMethyl mercury (MeHg) is highly neurotoxic, affecting visual function in addition to other central nervous system functions. The effect of mercury intoxication on the amplitude of horizontal cell responses to light was studied in the retina of the fish Hoplias malabaricus. Intracellular responses were recorded from horizontal cells of fish previously intoxicated with MeHg by intraperitoneal injection (IP group) or by trophic exposure (T group). Only one retina per fish was used. The doses of MeHg chloride administered to the IP group were 0.01, 0.05, 0.1, 1.0, 2.0, and 6.0 mg/kg. The amplitudes of the horizontal cell responses were lower than control in individuals exposed to 0.01 (N = 4 retinas), 0.05 (N = 2 retinas) and 0.1 mg/kg (N = 1 retina), whereas no responses were recorded in the 1.0, 2.0, and 6.0 mg/kg groups. T group individuals were fed young specimens of Astyanax sp previously injected with MeHg corresponding to 0.75 (N = 1 retina), 0.075 (N = 8 retinas) or 0.0075 (N = 4 retinas) mg/kg fish body weight. After 14 doses, one every 5 days, the amplitude of the horizontal cell response was higher than control in individuals exposed to 0.075 and 0.0075 mg/kg, and lower in individuals exposed to 0.75 mg/kg. We conclude that intoxication with MeHg affects the electrophysiological response of the horizontal cells in the retina, either reducing or increasing its amplitude compared to control, and that these effects are related to the dose and/or to the mode of administration.Univ São Paulo, Inst Psicol, Dept Psicol Expt, Ctr Neurociencias & Comportamento, BR-05508900 São Paulo, BrazilUniversidade Federal do Paraná (UFPF), Dept Biol Celular, BR-80060000 Curitiba, Parana, BrazilUNESP, Dept Psicol, Univ Estadual Paulista Julio de Mesquita Filho, Bauru, SP, BrazilUNESP, Dept Psicol, Univ Estadual Paulista Julio de Mesquita Filho, Bauru, SP, BrazilAssociação Brasileira de Divulgação Científica (ABRADIC)Universidade de São Paulo (USP)Universidade Federal do Paraná (UFPR)Universidade Estadual Paulista (Unesp)Tanan, C. L.Ventura, D. F.de Souza, J. M.Grotzner, S. R.Mela, M.Gouveia, A. [UNESP]Oliveira-Ribeiro, C. A.2014-05-20T13:26:38Z2014-05-20T13:26:38Z2006-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article987-995application/pdfhttp://dx.doi.org/10.1590/S0100-879X2006000700017Brazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 39, n. 7, p. 987-995, 2006.0100-879Xhttp://hdl.handle.net/11449/862110.1590/S0100-879X2006000700017S0100-879X2006000700017WOS:0002396242000172-s2.0-33746345629WOS000239624200017.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Research1.492info:eu-repo/semantics/openAccess2024-04-29T15:53:53Zoai:repositorio.unesp.br:11449/8621Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:27:05.313974Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus) |
title |
Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus) |
spellingShingle |
Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus) Tanan, C. L. methyl mercury horizontal cells electrophysiology Hoplias malabaricus retina |
title_short |
Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus) |
title_full |
Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus) |
title_fullStr |
Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus) |
title_full_unstemmed |
Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus) |
title_sort |
Effects of mercury intoxication on the response of horizontal cells of the retina of thraira fish (Hoplias malabaricus) |
author |
Tanan, C. L. |
author_facet |
Tanan, C. L. Ventura, D. F. de Souza, J. M. Grotzner, S. R. Mela, M. Gouveia, A. [UNESP] Oliveira-Ribeiro, C. A. |
author_role |
author |
author2 |
Ventura, D. F. de Souza, J. M. Grotzner, S. R. Mela, M. Gouveia, A. [UNESP] Oliveira-Ribeiro, C. A. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal do Paraná (UFPR) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Tanan, C. L. Ventura, D. F. de Souza, J. M. Grotzner, S. R. Mela, M. Gouveia, A. [UNESP] Oliveira-Ribeiro, C. A. |
dc.subject.por.fl_str_mv |
methyl mercury horizontal cells electrophysiology Hoplias malabaricus retina |
topic |
methyl mercury horizontal cells electrophysiology Hoplias malabaricus retina |
description |
Methyl mercury (MeHg) is highly neurotoxic, affecting visual function in addition to other central nervous system functions. The effect of mercury intoxication on the amplitude of horizontal cell responses to light was studied in the retina of the fish Hoplias malabaricus. Intracellular responses were recorded from horizontal cells of fish previously intoxicated with MeHg by intraperitoneal injection (IP group) or by trophic exposure (T group). Only one retina per fish was used. The doses of MeHg chloride administered to the IP group were 0.01, 0.05, 0.1, 1.0, 2.0, and 6.0 mg/kg. The amplitudes of the horizontal cell responses were lower than control in individuals exposed to 0.01 (N = 4 retinas), 0.05 (N = 2 retinas) and 0.1 mg/kg (N = 1 retina), whereas no responses were recorded in the 1.0, 2.0, and 6.0 mg/kg groups. T group individuals were fed young specimens of Astyanax sp previously injected with MeHg corresponding to 0.75 (N = 1 retina), 0.075 (N = 8 retinas) or 0.0075 (N = 4 retinas) mg/kg fish body weight. After 14 doses, one every 5 days, the amplitude of the horizontal cell response was higher than control in individuals exposed to 0.075 and 0.0075 mg/kg, and lower in individuals exposed to 0.75 mg/kg. We conclude that intoxication with MeHg affects the electrophysiological response of the horizontal cells in the retina, either reducing or increasing its amplitude compared to control, and that these effects are related to the dose and/or to the mode of administration. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-07-01 2014-05-20T13:26:38Z 2014-05-20T13:26:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X2006000700017 Brazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 39, n. 7, p. 987-995, 2006. 0100-879X http://hdl.handle.net/11449/8621 10.1590/S0100-879X2006000700017 S0100-879X2006000700017 WOS:000239624200017 2-s2.0-33746345629 WOS000239624200017.pdf |
url |
http://dx.doi.org/10.1590/S0100-879X2006000700017 http://hdl.handle.net/11449/8621 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 39, n. 7, p. 987-995, 2006. 0100-879X 10.1590/S0100-879X2006000700017 S0100-879X2006000700017 WOS:000239624200017 2-s2.0-33746345629 WOS000239624200017.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research 1.492 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
987-995 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica (ABRADIC) |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica (ABRADIC) |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128654117437440 |