Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycle
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0002313 http://hdl.handle.net/11449/18009 |
Resumo: | Parasite virulence genes are usually associated with telomeres. The clustering of the telomeres, together with their particular spatial distribution in the nucleus of human parasites such as Plasmodium falciparum and Trypanosoma brucei, has been suggested to play a role in facilitating ectopic recombination and in the emergence of new antigenic variants. Leishmania parasites, as well as other trypanosomes, have unusual gene expression characteristics, such as polycistronic and constitutive transcription of protein-coding genes. Leishmania subtelomeric regions are even more unique because unlike these regions in other trypanosomes they are devoid of virulence genes. Given these peculiarities of Leishmania, we sought to investigate how telomeres are organized in the nucleus of Leishmania major parasites at both the human and insect stages of their life cycle. We developed a new automated and precise method for identifying telomere position in the three-dimensional space of the nucleus, and we found that the telomeres are organized in clusters present in similar numbers in both the human and insect stages. While the number of clusters remained the same, their distribution differed between the two stages. The telomeric clusters were found more concentrated near the center of the nucleus in the human stage than in the insect stage suggesting reorganization during the parasite's differentiation process between the two hosts. These data provide the first 3D analysis of Leishmania telomere organization. The possible biological implications of these findings are discussed. |
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Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life CycleParasite virulence genes are usually associated with telomeres. The clustering of the telomeres, together with their particular spatial distribution in the nucleus of human parasites such as Plasmodium falciparum and Trypanosoma brucei, has been suggested to play a role in facilitating ectopic recombination and in the emergence of new antigenic variants. Leishmania parasites, as well as other trypanosomes, have unusual gene expression characteristics, such as polycistronic and constitutive transcription of protein-coding genes. Leishmania subtelomeric regions are even more unique because unlike these regions in other trypanosomes they are devoid of virulence genes. Given these peculiarities of Leishmania, we sought to investigate how telomeres are organized in the nucleus of Leishmania major parasites at both the human and insect stages of their life cycle. We developed a new automated and precise method for identifying telomere position in the three-dimensional space of the nucleus, and we found that the telomeres are organized in clusters present in similar numbers in both the human and insect stages. While the number of clusters remained the same, their distribution differed between the two stages. The telomeric clusters were found more concentrated near the center of the nucleus in the human stage than in the insect stage suggesting reorganization during the parasite's differentiation process between the two hosts. These data provide the first 3D analysis of Leishmania telomere organization. The possible biological implications of these findings are discussed.Institut Pasteur KoreaInst Pasteur Korea, Syst Biol Pathogens Grp, Seoul, South KoreaInst Pasteur Korea, Image Mining Grp, Seoul, South KoreaUniv Fed São Paulo, Dept Microbiol, Imunol Parasitol, São Paulo, BrazilUniv Estadual Paulista, Inst Biocien, Dept Genet, São Paulo, BrazilUniv Estadual Paulista, Inst Biocien, Dept Genet, São Paulo, BrazilPublic Library ScienceInst Pasteur KoreaUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Dossin, Fernando de M.Dufour, AlexandreDusch, ElodieSiqueira-Neto, Jair L.Moraes, Carolina B.Yang, Gyong SeonCano, Maria Isabel [UNESP]Genovesio, AugusteFreitas-Junior, Lucio H.2014-05-20T13:50:27Z2014-05-20T13:50:27Z2008-06-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6application/pdfhttp://dx.doi.org/10.1371/journal.pone.0002313Plos One. San Francisco: Public Library Science, v. 3, n. 6, p. 6, 2008.1932-6203http://hdl.handle.net/11449/1800910.1371/journal.pone.0002313WOS:000263248800003WOS000263248800003.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2023-10-01T06:06:08Zoai:repositorio.unesp.br:11449/18009Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-01T06:06:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycle |
title |
Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycle |
spellingShingle |
Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycle Dossin, Fernando de M. |
title_short |
Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycle |
title_full |
Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycle |
title_fullStr |
Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycle |
title_full_unstemmed |
Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycle |
title_sort |
Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycle |
author |
Dossin, Fernando de M. |
author_facet |
Dossin, Fernando de M. Dufour, Alexandre Dusch, Elodie Siqueira-Neto, Jair L. Moraes, Carolina B. Yang, Gyong Seon Cano, Maria Isabel [UNESP] Genovesio, Auguste Freitas-Junior, Lucio H. |
author_role |
author |
author2 |
Dufour, Alexandre Dusch, Elodie Siqueira-Neto, Jair L. Moraes, Carolina B. Yang, Gyong Seon Cano, Maria Isabel [UNESP] Genovesio, Auguste Freitas-Junior, Lucio H. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Inst Pasteur Korea Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Dossin, Fernando de M. Dufour, Alexandre Dusch, Elodie Siqueira-Neto, Jair L. Moraes, Carolina B. Yang, Gyong Seon Cano, Maria Isabel [UNESP] Genovesio, Auguste Freitas-Junior, Lucio H. |
description |
Parasite virulence genes are usually associated with telomeres. The clustering of the telomeres, together with their particular spatial distribution in the nucleus of human parasites such as Plasmodium falciparum and Trypanosoma brucei, has been suggested to play a role in facilitating ectopic recombination and in the emergence of new antigenic variants. Leishmania parasites, as well as other trypanosomes, have unusual gene expression characteristics, such as polycistronic and constitutive transcription of protein-coding genes. Leishmania subtelomeric regions are even more unique because unlike these regions in other trypanosomes they are devoid of virulence genes. Given these peculiarities of Leishmania, we sought to investigate how telomeres are organized in the nucleus of Leishmania major parasites at both the human and insect stages of their life cycle. We developed a new automated and precise method for identifying telomere position in the three-dimensional space of the nucleus, and we found that the telomeres are organized in clusters present in similar numbers in both the human and insect stages. While the number of clusters remained the same, their distribution differed between the two stages. The telomeric clusters were found more concentrated near the center of the nucleus in the human stage than in the insect stage suggesting reorganization during the parasite's differentiation process between the two hosts. These data provide the first 3D analysis of Leishmania telomere organization. The possible biological implications of these findings are discussed. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-06-11 2014-05-20T13:50:27Z 2014-05-20T13:50:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0002313 Plos One. San Francisco: Public Library Science, v. 3, n. 6, p. 6, 2008. 1932-6203 http://hdl.handle.net/11449/18009 10.1371/journal.pone.0002313 WOS:000263248800003 WOS000263248800003.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0002313 http://hdl.handle.net/11449/18009 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 3, n. 6, p. 6, 2008. 1932-6203 10.1371/journal.pone.0002313 WOS:000263248800003 WOS000263248800003.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
6 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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