Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1177/1098612X16680697 http://hdl.handle.net/11449/163645 |
Resumo: | Objectives The present study investigated the effect of contraceptive treatment with deslorelin acetate on in vitro embryo production and oocyte recovery in domestic queens. Methods Twenty-one mature domestic cats were used. Eleven queens (treated group) and one tom were kept in an experimental cattery, and 10 queens were privately owned (control group). When in interestrus or diestrus (day 0) a deslorelin acetate implant (Suprelorin, 4.7 mg/animal) was inserted into the subcutaneous tissue of the interscapular region in all queens in the treated group. After 6 months of treatment, all animals were ovariohysterectomized, and the ovaries were used for in vitro embryo production. Percentage of cleavage was determined 18 h after oocyte insemination and blastocyst formation was assessed on the eighth day of culture. The rate of cumulus-oocyte complexes (COCs) recovery was analyzed by an unpaired t-test. The cleavage and blastocyst rates were expressed as percentages and analyzed by Fisher's exact test. All analyses were performed using GraphPad Prism v5.0, with P <0.05 set as the level of significance. Results In the treated group, we recovered 8.3 +/- 1.15 grade I COCs per queen; the cleavage rate was 60% and the blastocyst rate was 36%. In the control group, we recovered 18.4 +/- 3.21 grade I COCs per queen; the cleavage rate was 55.97% and the blastocyst rate was 34%. Forty percent of treated females did not produce any blastocysts. In the treated group, we observed a significant decrease in COC recovery. Although there was no significant difference in cleavage and blastocyst rates between groups, 40% of treated females did not produce any blastocysts. Conclusions Recovery of grade I COCs is negatively affected by deslorelin treatment in domestic cats. Regarding embryo production, new studies are still necessary to evaluate the success of this technique owing to the individual effect caused by deslorelin acetate. |
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Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic catsObjectives The present study investigated the effect of contraceptive treatment with deslorelin acetate on in vitro embryo production and oocyte recovery in domestic queens. Methods Twenty-one mature domestic cats were used. Eleven queens (treated group) and one tom were kept in an experimental cattery, and 10 queens were privately owned (control group). When in interestrus or diestrus (day 0) a deslorelin acetate implant (Suprelorin, 4.7 mg/animal) was inserted into the subcutaneous tissue of the interscapular region in all queens in the treated group. After 6 months of treatment, all animals were ovariohysterectomized, and the ovaries were used for in vitro embryo production. Percentage of cleavage was determined 18 h after oocyte insemination and blastocyst formation was assessed on the eighth day of culture. The rate of cumulus-oocyte complexes (COCs) recovery was analyzed by an unpaired t-test. The cleavage and blastocyst rates were expressed as percentages and analyzed by Fisher's exact test. All analyses were performed using GraphPad Prism v5.0, with P <0.05 set as the level of significance. Results In the treated group, we recovered 8.3 +/- 1.15 grade I COCs per queen; the cleavage rate was 60% and the blastocyst rate was 36%. In the control group, we recovered 18.4 +/- 3.21 grade I COCs per queen; the cleavage rate was 55.97% and the blastocyst rate was 34%. Forty percent of treated females did not produce any blastocysts. In the treated group, we observed a significant decrease in COC recovery. Although there was no significant difference in cleavage and blastocyst rates between groups, 40% of treated females did not produce any blastocysts. Conclusions Recovery of grade I COCs is negatively affected by deslorelin treatment in domestic cats. Regarding embryo production, new studies are still necessary to evaluate the success of this technique owing to the individual effect caused by deslorelin acetate.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Fac Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, BrazilSao Paulo State Univ, Fac Vet Med & Anim Sci, Dept Vet Surg & Anesthesiol, Botucatu, SP, BrazilSao Paulo State Univ, Fac Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, BrazilSao Paulo State Univ, Fac Vet Med & Anim Sci, Dept Vet Surg & Anesthesiol, Botucatu, SP, BrazilFAPESP: 2011/23318-0FAPESP: 2012/09002-3Sage Publications LtdUniversidade Estadual Paulista (Unesp)Ackermann, Camila Louise [UNESP]Trevisol, Eduardo [UNESP]Crocomo, Leticia Ferrari [UNESP]Rascado, Tatiana da Silva [UNESP]Volpato, Rodrigo [UNESP]Freitas Guaitolini, Carlos Renato de [UNESP]Lopes, Carlize [UNESP]Costa, Talita de Almeida [UNESP]Lopes, Maria Denise [UNESP]2018-11-26T17:44:24Z2018-11-26T17:44:24Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1091-1095application/pdfhttp://dx.doi.org/10.1177/1098612X16680697Journal Of Feline Medicine And Surgery. London: Sage Publications Ltd, v. 19, n. 10, p. 1091-1095, 2017.1098-612Xhttp://hdl.handle.net/11449/16364510.1177/1098612X16680697WOS:000418287900015WOS000418287900015.pdf6666129914663018Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Feline Medicine And Surgeryinfo:eu-repo/semantics/openAccess2024-09-09T14:00:33Zoai:repositorio.unesp.br:11449/163645Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:00:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats |
title |
Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats |
spellingShingle |
Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats Ackermann, Camila Louise [UNESP] |
title_short |
Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats |
title_full |
Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats |
title_fullStr |
Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats |
title_full_unstemmed |
Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats |
title_sort |
Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats |
author |
Ackermann, Camila Louise [UNESP] |
author_facet |
Ackermann, Camila Louise [UNESP] Trevisol, Eduardo [UNESP] Crocomo, Leticia Ferrari [UNESP] Rascado, Tatiana da Silva [UNESP] Volpato, Rodrigo [UNESP] Freitas Guaitolini, Carlos Renato de [UNESP] Lopes, Carlize [UNESP] Costa, Talita de Almeida [UNESP] Lopes, Maria Denise [UNESP] |
author_role |
author |
author2 |
Trevisol, Eduardo [UNESP] Crocomo, Leticia Ferrari [UNESP] Rascado, Tatiana da Silva [UNESP] Volpato, Rodrigo [UNESP] Freitas Guaitolini, Carlos Renato de [UNESP] Lopes, Carlize [UNESP] Costa, Talita de Almeida [UNESP] Lopes, Maria Denise [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ackermann, Camila Louise [UNESP] Trevisol, Eduardo [UNESP] Crocomo, Leticia Ferrari [UNESP] Rascado, Tatiana da Silva [UNESP] Volpato, Rodrigo [UNESP] Freitas Guaitolini, Carlos Renato de [UNESP] Lopes, Carlize [UNESP] Costa, Talita de Almeida [UNESP] Lopes, Maria Denise [UNESP] |
description |
Objectives The present study investigated the effect of contraceptive treatment with deslorelin acetate on in vitro embryo production and oocyte recovery in domestic queens. Methods Twenty-one mature domestic cats were used. Eleven queens (treated group) and one tom were kept in an experimental cattery, and 10 queens were privately owned (control group). When in interestrus or diestrus (day 0) a deslorelin acetate implant (Suprelorin, 4.7 mg/animal) was inserted into the subcutaneous tissue of the interscapular region in all queens in the treated group. After 6 months of treatment, all animals were ovariohysterectomized, and the ovaries were used for in vitro embryo production. Percentage of cleavage was determined 18 h after oocyte insemination and blastocyst formation was assessed on the eighth day of culture. The rate of cumulus-oocyte complexes (COCs) recovery was analyzed by an unpaired t-test. The cleavage and blastocyst rates were expressed as percentages and analyzed by Fisher's exact test. All analyses were performed using GraphPad Prism v5.0, with P <0.05 set as the level of significance. Results In the treated group, we recovered 8.3 +/- 1.15 grade I COCs per queen; the cleavage rate was 60% and the blastocyst rate was 36%. In the control group, we recovered 18.4 +/- 3.21 grade I COCs per queen; the cleavage rate was 55.97% and the blastocyst rate was 34%. Forty percent of treated females did not produce any blastocysts. In the treated group, we observed a significant decrease in COC recovery. Although there was no significant difference in cleavage and blastocyst rates between groups, 40% of treated females did not produce any blastocysts. Conclusions Recovery of grade I COCs is negatively affected by deslorelin treatment in domestic cats. Regarding embryo production, new studies are still necessary to evaluate the success of this technique owing to the individual effect caused by deslorelin acetate. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-01 2018-11-26T17:44:24Z 2018-11-26T17:44:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1177/1098612X16680697 Journal Of Feline Medicine And Surgery. London: Sage Publications Ltd, v. 19, n. 10, p. 1091-1095, 2017. 1098-612X http://hdl.handle.net/11449/163645 10.1177/1098612X16680697 WOS:000418287900015 WOS000418287900015.pdf 6666129914663018 |
url |
http://dx.doi.org/10.1177/1098612X16680697 http://hdl.handle.net/11449/163645 |
identifier_str_mv |
Journal Of Feline Medicine And Surgery. London: Sage Publications Ltd, v. 19, n. 10, p. 1091-1095, 2017. 1098-612X 10.1177/1098612X16680697 WOS:000418287900015 WOS000418287900015.pdf 6666129914663018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Feline Medicine And Surgery |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1091-1095 application/pdf |
dc.publisher.none.fl_str_mv |
Sage Publications Ltd |
publisher.none.fl_str_mv |
Sage Publications Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021310842011648 |