Sodium caseinate improves longevity and fertility of frozen bull semen

Detalhes bibliográficos
Autor(a) principal: Alves Diniz, Jefferson Viana
Data de Publicação: 2020
Outros Autores: Satrapa, Rafael Augusto, Segabinazzi, Lorenzo Garrido T. M. [UNESP], Matos Carneiro, Joao Alexandre [UNESP], Oba, Eunice [UNESP], Papa, Federico Ozanam [UNESP], Freitas Dell'Aqua, Camila de Paula [UNESP], Loureiro, Barbara, Dell'Aqua Junior, Jose Antonio [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.theriogenology.2020.05.028
http://hdl.handle.net/11449/197251
Resumo: This study aimed to evaluate the effect of sodium caseinate added into freezing extender on the sperm parameters of cryopreserved bull semen and in vitro and in vivo fertility. One ejaculate of 30 bulls was used and processed using Botu-Bov (Botupharma, Botucatu, Brazil) with the addition of 20% egg yolk (EY) or 15% egg yolk with 2% sodium caseinate (EY thorn SC), subsequently submitted to freezing. Semen from both groups were evaluated immediately after thawing (T0) and after thermic stress at 37 degrees C for 90 min (T90), for sperm kinetics, by CASA method, and plasma membrane integrity (PMI), superoxide (O-2) concentration and high mitochondrial potential (HMP) by flow cytometry. In vitro fertilization (IVF) was performed to assess embryo cleavage rate on day 3, and blastocyst rate on day 8. The in vivo fertility test was performed using fixed-time artificial insemination (FTAI). In sperm evaluation, trajectory velocity, linear velocity, curvilinear velocity, and lateral head movement were higher (P < 0.05) in EY thorn SC at T0. At T90, while rectilinearity and linearity did not differ between EY and EY thorn SC (P > 0.05), the other parameters evaluated were higher in EY thorn SC. Similarly, the integrity of the plasma and acrosomal membranes (iPAM) was higher (P < 0.05) at T90 in EY thorn SC, but did not differ (P > 0.05) between the groups at T0. For O-2(-) and HMP, the values were lower (P < 0.05) in EY thorn SC group in both moments; furthermore, EY thorn SC showed higher cleavage and blastocyst rates in IVF. Likewise, pregnancy rates by FTAI were higher (P < 0.05) in the EY thorn SC group. In conclusion, the addition of sodium caseinate into freezing extender improves sperm parameters of frozen-thawed bull semen and fertility rates on during in vitro and in vivo tests. (c) 2020 Elsevier Inc. All rights reserved.
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spelling Sodium caseinate improves longevity and fertility of frozen bull semenBull semenFTAIFrozen semenCryopreservationIVPThis study aimed to evaluate the effect of sodium caseinate added into freezing extender on the sperm parameters of cryopreserved bull semen and in vitro and in vivo fertility. One ejaculate of 30 bulls was used and processed using Botu-Bov (Botupharma, Botucatu, Brazil) with the addition of 20% egg yolk (EY) or 15% egg yolk with 2% sodium caseinate (EY thorn SC), subsequently submitted to freezing. Semen from both groups were evaluated immediately after thawing (T0) and after thermic stress at 37 degrees C for 90 min (T90), for sperm kinetics, by CASA method, and plasma membrane integrity (PMI), superoxide (O-2) concentration and high mitochondrial potential (HMP) by flow cytometry. In vitro fertilization (IVF) was performed to assess embryo cleavage rate on day 3, and blastocyst rate on day 8. The in vivo fertility test was performed using fixed-time artificial insemination (FTAI). In sperm evaluation, trajectory velocity, linear velocity, curvilinear velocity, and lateral head movement were higher (P < 0.05) in EY thorn SC at T0. At T90, while rectilinearity and linearity did not differ between EY and EY thorn SC (P > 0.05), the other parameters evaluated were higher in EY thorn SC. Similarly, the integrity of the plasma and acrosomal membranes (iPAM) was higher (P < 0.05) at T90 in EY thorn SC, but did not differ (P > 0.05) between the groups at T0. For O-2(-) and HMP, the values were lower (P < 0.05) in EY thorn SC group in both moments; furthermore, EY thorn SC showed higher cleavage and blastocyst rates in IVF. Likewise, pregnancy rates by FTAI were higher (P < 0.05) in the EY thorn SC group. In conclusion, the addition of sodium caseinate into freezing extender improves sperm parameters of frozen-thawed bull semen and fertility rates on during in vitro and in vivo tests. (c) 2020 Elsevier Inc. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Acre, Programa Posgrad Sanidade & Prod Anim Sustentcive, Rodovia BR 364,Km 4, BR-69920900 Acre, AC, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Reprod Anim & Radiol Vet, Rua Prof Doutor Walter Mauricio Correa S-N, BR-18618681 Sao Paulo, SP, BrazilUniv Vila Velha, Lab Reprod Physiol, Rua Viana S-N, BR-29102920 Vila Velha, ES, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Reprod Anim & Radiol Vet, Rua Prof Doutor Walter Mauricio Correa S-N, BR-18618681 Sao Paulo, SP, BrazilElsevier B.V.Univ Fed AcreUniversidade Estadual Paulista (Unesp)Univ Vila VelhaAlves Diniz, Jefferson VianaSatrapa, Rafael AugustoSegabinazzi, Lorenzo Garrido T. M. [UNESP]Matos Carneiro, Joao Alexandre [UNESP]Oba, Eunice [UNESP]Papa, Federico Ozanam [UNESP]Freitas Dell'Aqua, Camila de Paula [UNESP]Loureiro, BarbaraDell'Aqua Junior, Jose Antonio [UNESP]2020-12-10T20:10:55Z2020-12-10T20:10:55Z2020-09-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article59-65http://dx.doi.org/10.1016/j.theriogenology.2020.05.028Theriogenology. New York: Elsevier Science Inc, v. 154, p. 59-65, 2020.0093-691Xhttp://hdl.handle.net/11449/19725110.1016/j.theriogenology.2020.05.028WOS:000564551700008Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2024-09-09T14:01:21Zoai:repositorio.unesp.br:11449/197251Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Sodium caseinate improves longevity and fertility of frozen bull semen
title Sodium caseinate improves longevity and fertility of frozen bull semen
spellingShingle Sodium caseinate improves longevity and fertility of frozen bull semen
Alves Diniz, Jefferson Viana
Bull semen
FTAI
Frozen semen
Cryopreservation
IVP
title_short Sodium caseinate improves longevity and fertility of frozen bull semen
title_full Sodium caseinate improves longevity and fertility of frozen bull semen
title_fullStr Sodium caseinate improves longevity and fertility of frozen bull semen
title_full_unstemmed Sodium caseinate improves longevity and fertility of frozen bull semen
title_sort Sodium caseinate improves longevity and fertility of frozen bull semen
author Alves Diniz, Jefferson Viana
author_facet Alves Diniz, Jefferson Viana
Satrapa, Rafael Augusto
Segabinazzi, Lorenzo Garrido T. M. [UNESP]
Matos Carneiro, Joao Alexandre [UNESP]
Oba, Eunice [UNESP]
Papa, Federico Ozanam [UNESP]
Freitas Dell'Aqua, Camila de Paula [UNESP]
Loureiro, Barbara
Dell'Aqua Junior, Jose Antonio [UNESP]
author_role author
author2 Satrapa, Rafael Augusto
Segabinazzi, Lorenzo Garrido T. M. [UNESP]
Matos Carneiro, Joao Alexandre [UNESP]
Oba, Eunice [UNESP]
Papa, Federico Ozanam [UNESP]
Freitas Dell'Aqua, Camila de Paula [UNESP]
Loureiro, Barbara
Dell'Aqua Junior, Jose Antonio [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Fed Acre
Universidade Estadual Paulista (Unesp)
Univ Vila Velha
dc.contributor.author.fl_str_mv Alves Diniz, Jefferson Viana
Satrapa, Rafael Augusto
Segabinazzi, Lorenzo Garrido T. M. [UNESP]
Matos Carneiro, Joao Alexandre [UNESP]
Oba, Eunice [UNESP]
Papa, Federico Ozanam [UNESP]
Freitas Dell'Aqua, Camila de Paula [UNESP]
Loureiro, Barbara
Dell'Aqua Junior, Jose Antonio [UNESP]
dc.subject.por.fl_str_mv Bull semen
FTAI
Frozen semen
Cryopreservation
IVP
topic Bull semen
FTAI
Frozen semen
Cryopreservation
IVP
description This study aimed to evaluate the effect of sodium caseinate added into freezing extender on the sperm parameters of cryopreserved bull semen and in vitro and in vivo fertility. One ejaculate of 30 bulls was used and processed using Botu-Bov (Botupharma, Botucatu, Brazil) with the addition of 20% egg yolk (EY) or 15% egg yolk with 2% sodium caseinate (EY thorn SC), subsequently submitted to freezing. Semen from both groups were evaluated immediately after thawing (T0) and after thermic stress at 37 degrees C for 90 min (T90), for sperm kinetics, by CASA method, and plasma membrane integrity (PMI), superoxide (O-2) concentration and high mitochondrial potential (HMP) by flow cytometry. In vitro fertilization (IVF) was performed to assess embryo cleavage rate on day 3, and blastocyst rate on day 8. The in vivo fertility test was performed using fixed-time artificial insemination (FTAI). In sperm evaluation, trajectory velocity, linear velocity, curvilinear velocity, and lateral head movement were higher (P < 0.05) in EY thorn SC at T0. At T90, while rectilinearity and linearity did not differ between EY and EY thorn SC (P > 0.05), the other parameters evaluated were higher in EY thorn SC. Similarly, the integrity of the plasma and acrosomal membranes (iPAM) was higher (P < 0.05) at T90 in EY thorn SC, but did not differ (P > 0.05) between the groups at T0. For O-2(-) and HMP, the values were lower (P < 0.05) in EY thorn SC group in both moments; furthermore, EY thorn SC showed higher cleavage and blastocyst rates in IVF. Likewise, pregnancy rates by FTAI were higher (P < 0.05) in the EY thorn SC group. In conclusion, the addition of sodium caseinate into freezing extender improves sperm parameters of frozen-thawed bull semen and fertility rates on during in vitro and in vivo tests. (c) 2020 Elsevier Inc. All rights reserved.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-10T20:10:55Z
2020-12-10T20:10:55Z
2020-09-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.theriogenology.2020.05.028
Theriogenology. New York: Elsevier Science Inc, v. 154, p. 59-65, 2020.
0093-691X
http://hdl.handle.net/11449/197251
10.1016/j.theriogenology.2020.05.028
WOS:000564551700008
url http://dx.doi.org/10.1016/j.theriogenology.2020.05.028
http://hdl.handle.net/11449/197251
identifier_str_mv Theriogenology. New York: Elsevier Science Inc, v. 154, p. 59-65, 2020.
0093-691X
10.1016/j.theriogenology.2020.05.028
WOS:000564551700008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Theriogenology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 59-65
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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