aPDT for periodontitis treatment in ovariectomized rats under systemic nicotine
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2018.02.017 http://hdl.handle.net/11449/176020 |
Resumo: | Background: This study evaluated the antimicrobial photodynamic therapy (aPDT) as an adjunctive therapy to scaling and root planning (SRP) for the treatment of experimental periodontitis (EP) in ovariectomized rats under systemic nicotine. Methods: Female ovariectomized rats (n = 180) were divided into two groups: vehicle administration (Veh) and nicotine administration (Nic). Mini-pumps containing either vehicle or nicotine were inserted in the rats 30 days before the induction of EP, which was induced by placing a ligature around the left mandibular first molar. The rats were randomly divided into three treatment subgroups: SRP, SRP plus low-level laser therapy (LLLT), and SRP plus aPDT. aPDT consisted of the application of a phenothiazine photosensitizer followed by LLLT. Ten animals from each group were euthanized at days 7, 15, and 30 after periodontal treatment. The furcation region was evaluated using histological, histometric analyses and immunolabelling for PCNA, TRAP, RANKL, and OPG. Results: Nicotine administration resulted in greater bone loss (BL). aPDT resulted in lower BL compared to SRP. aPDT showed higher quantities of PCNA-positive cells compared to SRP, regardless of the nicotine status. aPDT resulted in less recruitment of osteoclasts and lower RANKL immunolabelling compared to LLLT and SRP. Conclusion: aPDT was effective in animals treated with nicotine. |
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aPDT for periodontitis treatment in ovariectomized rats under systemic nicotineNicotineOvariectomyPeriodontitisPhotochemotherapyRANK ligandBackground: This study evaluated the antimicrobial photodynamic therapy (aPDT) as an adjunctive therapy to scaling and root planning (SRP) for the treatment of experimental periodontitis (EP) in ovariectomized rats under systemic nicotine. Methods: Female ovariectomized rats (n = 180) were divided into two groups: vehicle administration (Veh) and nicotine administration (Nic). Mini-pumps containing either vehicle or nicotine were inserted in the rats 30 days before the induction of EP, which was induced by placing a ligature around the left mandibular first molar. The rats were randomly divided into three treatment subgroups: SRP, SRP plus low-level laser therapy (LLLT), and SRP plus aPDT. aPDT consisted of the application of a phenothiazine photosensitizer followed by LLLT. Ten animals from each group were euthanized at days 7, 15, and 30 after periodontal treatment. The furcation region was evaluated using histological, histometric analyses and immunolabelling for PCNA, TRAP, RANKL, and OPG. Results: Nicotine administration resulted in greater bone loss (BL). aPDT resulted in lower BL compared to SRP. aPDT showed higher quantities of PCNA-positive cells compared to SRP, regardless of the nicotine status. aPDT resulted in less recruitment of osteoclasts and lower RANKL immunolabelling compared to LLLT and SRP. Conclusion: aPDT was effective in animals treated with nicotine.São Paulo State University (UNESP) Group for the Research and Study of Laser in Dentistry Department of Surgery and Integrated Clinic Division of Periodontics School of Dentistry, Araçatuba, 1193, Jose BonifácioSão Paulo State University (UNESP) Department of Basic Science School of Dentistry, Araçatuba, 1193, José BonifácioSão Paulo State University (UNESP) Group for the Research and Study of Laser in Dentistry Department of Surgery and Integrated Clinic Division of Periodontics School of Dentistry, Araçatuba, 1193, Jose BonifácioSão Paulo State University (UNESP) Department of Basic Science School of Dentistry, Araçatuba, 1193, José BonifácioUniversidade Estadual Paulista (Unesp)Garcia, Valdir Gouveia [UNESP]Gualberto, Erivan Clementino [UNESP]Ervolino, Edilson [UNESP]Nagata, Maria José Hitomi [UNESP]de Almeida, Juliano Milanezi [UNESP]Theodoro, Letícia Helena [UNESP]2018-12-11T17:18:35Z2018-12-11T17:18:35Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article70-78application/pdfhttp://dx.doi.org/10.1016/j.pdpdt.2018.02.017Photodiagnosis and Photodynamic Therapy, v. 22, p. 70-78.1873-15971572-1000http://hdl.handle.net/11449/17602010.1016/j.pdpdt.2018.02.0172-s2.0-850441109002-s2.0-85044110900.pdf4408095517346846477444711812734683998700975720730000-0003-4859-0583Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapy0,647info:eu-repo/semantics/openAccess2024-01-04T06:27:26Zoai:repositorio.unesp.br:11449/176020Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-04T06:27:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
aPDT for periodontitis treatment in ovariectomized rats under systemic nicotine |
title |
aPDT for periodontitis treatment in ovariectomized rats under systemic nicotine |
spellingShingle |
aPDT for periodontitis treatment in ovariectomized rats under systemic nicotine Garcia, Valdir Gouveia [UNESP] Nicotine Ovariectomy Periodontitis Photochemotherapy RANK ligand |
title_short |
aPDT for periodontitis treatment in ovariectomized rats under systemic nicotine |
title_full |
aPDT for periodontitis treatment in ovariectomized rats under systemic nicotine |
title_fullStr |
aPDT for periodontitis treatment in ovariectomized rats under systemic nicotine |
title_full_unstemmed |
aPDT for periodontitis treatment in ovariectomized rats under systemic nicotine |
title_sort |
aPDT for periodontitis treatment in ovariectomized rats under systemic nicotine |
author |
Garcia, Valdir Gouveia [UNESP] |
author_facet |
Garcia, Valdir Gouveia [UNESP] Gualberto, Erivan Clementino [UNESP] Ervolino, Edilson [UNESP] Nagata, Maria José Hitomi [UNESP] de Almeida, Juliano Milanezi [UNESP] Theodoro, Letícia Helena [UNESP] |
author_role |
author |
author2 |
Gualberto, Erivan Clementino [UNESP] Ervolino, Edilson [UNESP] Nagata, Maria José Hitomi [UNESP] de Almeida, Juliano Milanezi [UNESP] Theodoro, Letícia Helena [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Garcia, Valdir Gouveia [UNESP] Gualberto, Erivan Clementino [UNESP] Ervolino, Edilson [UNESP] Nagata, Maria José Hitomi [UNESP] de Almeida, Juliano Milanezi [UNESP] Theodoro, Letícia Helena [UNESP] |
dc.subject.por.fl_str_mv |
Nicotine Ovariectomy Periodontitis Photochemotherapy RANK ligand |
topic |
Nicotine Ovariectomy Periodontitis Photochemotherapy RANK ligand |
description |
Background: This study evaluated the antimicrobial photodynamic therapy (aPDT) as an adjunctive therapy to scaling and root planning (SRP) for the treatment of experimental periodontitis (EP) in ovariectomized rats under systemic nicotine. Methods: Female ovariectomized rats (n = 180) were divided into two groups: vehicle administration (Veh) and nicotine administration (Nic). Mini-pumps containing either vehicle or nicotine were inserted in the rats 30 days before the induction of EP, which was induced by placing a ligature around the left mandibular first molar. The rats were randomly divided into three treatment subgroups: SRP, SRP plus low-level laser therapy (LLLT), and SRP plus aPDT. aPDT consisted of the application of a phenothiazine photosensitizer followed by LLLT. Ten animals from each group were euthanized at days 7, 15, and 30 after periodontal treatment. The furcation region was evaluated using histological, histometric analyses and immunolabelling for PCNA, TRAP, RANKL, and OPG. Results: Nicotine administration resulted in greater bone loss (BL). aPDT resulted in lower BL compared to SRP. aPDT showed higher quantities of PCNA-positive cells compared to SRP, regardless of the nicotine status. aPDT resulted in less recruitment of osteoclasts and lower RANKL immunolabelling compared to LLLT and SRP. Conclusion: aPDT was effective in animals treated with nicotine. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:18:35Z 2018-12-11T17:18:35Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2018.02.017 Photodiagnosis and Photodynamic Therapy, v. 22, p. 70-78. 1873-1597 1572-1000 http://hdl.handle.net/11449/176020 10.1016/j.pdpdt.2018.02.017 2-s2.0-85044110900 2-s2.0-85044110900.pdf 4408095517346846 4774447118127346 8399870097572073 0000-0003-4859-0583 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2018.02.017 http://hdl.handle.net/11449/176020 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 22, p. 70-78. 1873-1597 1572-1000 10.1016/j.pdpdt.2018.02.017 2-s2.0-85044110900 2-s2.0-85044110900.pdf 4408095517346846 4774447118127346 8399870097572073 0000-0003-4859-0583 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy 0,647 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
70-78 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965514889428992 |