Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0268973 http://hdl.handle.net/11449/241052 |
Resumo: | Considering the widespread use of rabbits in research that potentially causes pain and discomfort and the limited number of pain assessment validated tools in this species, we aimed to develop and validate a scale of acute postoperative pain in rabbits (RPBS). Footage of 58 rabbits from previous studies were used, recorded at ‘baseline’ (before orthopaedic and soft tissue surgeries), ‘pain’ (after surgery), ‘analgesia’ (after analgesic), and ‘24h post’ (24 hours after surgery). The videos were randomised and assessed twice by four evaluators, within one-month interval between evaluations. After content validation, RBPS was further refined using the criteria from the validation. According to the principal component analysis, RPBS was considered unidimensional. The intra- and inter-observer reliability was excellent (ICC>0.80) for all evaluators. There was a high Spearman’s correlation of the RPBS with unidimensional scales (>0.80) and a moderate correlation with the Rabbit Grimace Scale (0.68), confirming criterion validity. According to the mixed linear model, the scale was responsive, shown by the increase in pain scores after surgery. Construct validity was confirmed by known-group approach and internal relationships among items. Adequate item-total correlation (>0.3) was observed for all items, except for the attention to the affected area (0.04). The internal consistency was very good (Cronbach’s α coefficient = 0.78; Mcdonald’s ω coefficient = 0.83). The cut-off score for rescue analgesia was ≥3, with an area under the curve >0.95, demonstrating a high discriminatory capacity of the instrument. Scores 3 and 4 were within the uncertainty diagnostic zone. Specificity was 87% and sensitivity was 90%. It was concluded that the RPBS presented content, criterion, and construct validities, responsiveness, and reliability to assess acute pain in rabbits submitted to orthopaedic and soft tissue surgeries. The cut-off for rescue analgesia serves as a basis for the administration of analgesics to rabbits submitted to painful procedures. |
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Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus)Considering the widespread use of rabbits in research that potentially causes pain and discomfort and the limited number of pain assessment validated tools in this species, we aimed to develop and validate a scale of acute postoperative pain in rabbits (RPBS). Footage of 58 rabbits from previous studies were used, recorded at ‘baseline’ (before orthopaedic and soft tissue surgeries), ‘pain’ (after surgery), ‘analgesia’ (after analgesic), and ‘24h post’ (24 hours after surgery). The videos were randomised and assessed twice by four evaluators, within one-month interval between evaluations. After content validation, RBPS was further refined using the criteria from the validation. According to the principal component analysis, RPBS was considered unidimensional. The intra- and inter-observer reliability was excellent (ICC>0.80) for all evaluators. There was a high Spearman’s correlation of the RPBS with unidimensional scales (>0.80) and a moderate correlation with the Rabbit Grimace Scale (0.68), confirming criterion validity. According to the mixed linear model, the scale was responsive, shown by the increase in pain scores after surgery. Construct validity was confirmed by known-group approach and internal relationships among items. Adequate item-total correlation (>0.3) was observed for all items, except for the attention to the affected area (0.04). The internal consistency was very good (Cronbach’s α coefficient = 0.78; Mcdonald’s ω coefficient = 0.83). The cut-off score for rescue analgesia was ≥3, with an area under the curve >0.95, demonstrating a high discriminatory capacity of the instrument. Scores 3 and 4 were within the uncertainty diagnostic zone. Specificity was 87% and sensitivity was 90%. It was concluded that the RPBS presented content, criterion, and construct validities, responsiveness, and reliability to assess acute pain in rabbits submitted to orthopaedic and soft tissue surgeries. The cut-off for rescue analgesia serves as a basis for the administration of analgesics to rabbits submitted to painful procedures.Department of Surgical Specialties and Anesthesiology Botucatu Medical School São Paulo State University (Unesp), BotucatuDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (Unesp), BotucatuDepartment of Surgery School of Veterinary Medicine and Animal Science University of São Paulo (USP), São PauloDepartment of Veterinary Clinics and Surgery School of Agricultural and Veterinary Sciences São Paulo State University (Unesp), JaboticabalSchool of Health and Life Sciences Teesside UniversitySchool of Natural and Environmental Science Newcastle UniversityDepartment of Surgical Specialties and Anesthesiology Botucatu Medical School São Paulo State University (Unesp), BotucatuDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (Unesp), BotucatuDepartment of Veterinary Clinics and Surgery School of Agricultural and Veterinary Sciences São Paulo State University (Unesp), JaboticabalUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Teesside UniversityNewcastle UniversityPinho, Renata Haddad [UNESP]Luna, Stelio Pacca Loureiro [UNESP]Trindade, Pedro Henrique Esteves [UNESP]Justo, André AugustoCima, Daniela Santilli [UNESP]Fonseca, Mariana Werneck [UNESP]Minto, Bruno Watanabe [UNESP]Rocha, Fabiana Del Lama [UNESP]Miller, AmyFlecknell, PaulLeach, Matthew C.2023-03-01T20:44:51Z2023-03-01T20:44:51Z2022-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0268973PLoS ONE, v. 17, n. 5 5, 2022.1932-6203http://hdl.handle.net/11449/24105210.1371/journal.pone.02689732-s2.0-85130792645Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2024-09-09T14:01:21Zoai:repositorio.unesp.br:11449/241052Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus) |
title |
Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus) |
spellingShingle |
Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus) Pinho, Renata Haddad [UNESP] |
title_short |
Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus) |
title_full |
Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus) |
title_fullStr |
Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus) |
title_full_unstemmed |
Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus) |
title_sort |
Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus) |
author |
Pinho, Renata Haddad [UNESP] |
author_facet |
Pinho, Renata Haddad [UNESP] Luna, Stelio Pacca Loureiro [UNESP] Trindade, Pedro Henrique Esteves [UNESP] Justo, André Augusto Cima, Daniela Santilli [UNESP] Fonseca, Mariana Werneck [UNESP] Minto, Bruno Watanabe [UNESP] Rocha, Fabiana Del Lama [UNESP] Miller, Amy Flecknell, Paul Leach, Matthew C. |
author_role |
author |
author2 |
Luna, Stelio Pacca Loureiro [UNESP] Trindade, Pedro Henrique Esteves [UNESP] Justo, André Augusto Cima, Daniela Santilli [UNESP] Fonseca, Mariana Werneck [UNESP] Minto, Bruno Watanabe [UNESP] Rocha, Fabiana Del Lama [UNESP] Miller, Amy Flecknell, Paul Leach, Matthew C. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Teesside University Newcastle University |
dc.contributor.author.fl_str_mv |
Pinho, Renata Haddad [UNESP] Luna, Stelio Pacca Loureiro [UNESP] Trindade, Pedro Henrique Esteves [UNESP] Justo, André Augusto Cima, Daniela Santilli [UNESP] Fonseca, Mariana Werneck [UNESP] Minto, Bruno Watanabe [UNESP] Rocha, Fabiana Del Lama [UNESP] Miller, Amy Flecknell, Paul Leach, Matthew C. |
description |
Considering the widespread use of rabbits in research that potentially causes pain and discomfort and the limited number of pain assessment validated tools in this species, we aimed to develop and validate a scale of acute postoperative pain in rabbits (RPBS). Footage of 58 rabbits from previous studies were used, recorded at ‘baseline’ (before orthopaedic and soft tissue surgeries), ‘pain’ (after surgery), ‘analgesia’ (after analgesic), and ‘24h post’ (24 hours after surgery). The videos were randomised and assessed twice by four evaluators, within one-month interval between evaluations. After content validation, RBPS was further refined using the criteria from the validation. According to the principal component analysis, RPBS was considered unidimensional. The intra- and inter-observer reliability was excellent (ICC>0.80) for all evaluators. There was a high Spearman’s correlation of the RPBS with unidimensional scales (>0.80) and a moderate correlation with the Rabbit Grimace Scale (0.68), confirming criterion validity. According to the mixed linear model, the scale was responsive, shown by the increase in pain scores after surgery. Construct validity was confirmed by known-group approach and internal relationships among items. Adequate item-total correlation (>0.3) was observed for all items, except for the attention to the affected area (0.04). The internal consistency was very good (Cronbach’s α coefficient = 0.78; Mcdonald’s ω coefficient = 0.83). The cut-off score for rescue analgesia was ≥3, with an area under the curve >0.95, demonstrating a high discriminatory capacity of the instrument. Scores 3 and 4 were within the uncertainty diagnostic zone. Specificity was 87% and sensitivity was 90%. It was concluded that the RPBS presented content, criterion, and construct validities, responsiveness, and reliability to assess acute pain in rabbits submitted to orthopaedic and soft tissue surgeries. The cut-off for rescue analgesia serves as a basis for the administration of analgesics to rabbits submitted to painful procedures. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01 2023-03-01T20:44:51Z 2023-03-01T20:44:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0268973 PLoS ONE, v. 17, n. 5 5, 2022. 1932-6203 http://hdl.handle.net/11449/241052 10.1371/journal.pone.0268973 2-s2.0-85130792645 |
url |
http://dx.doi.org/10.1371/journal.pone.0268973 http://hdl.handle.net/11449/241052 |
identifier_str_mv |
PLoS ONE, v. 17, n. 5 5, 2022. 1932-6203 10.1371/journal.pone.0268973 2-s2.0-85130792645 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021327980986368 |