Estudio morfológico y morfométrico de las fibras musculares y de las intersecciones neuromusculares del músculo extensor largo de los dedos de ratas de edad avanzada sometidas a la restricción proteica materna
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4067/S0717-95022016000100057 http://hdl.handle.net/11449/220616 |
Resumo: | Protein restriction implies the functional involvement of several systems and organs, including the skeletal muscle, because it is a protein reservoir in the body. This study sought to analyze the morphological and morphometric features of the muscle fibers and neuromuscular junctions (NMJs) of the extensor digitorum longus (EDL) muscle in rats at 365 days of age, submitted to maternal protein restriction during the gestation and lactation periods. Wistar rats were divided into two groups: a Control Group mothers fed a normal-protein diet (17% protein) during pregnancy and lactation; and a Restricted Group - mothers fed a low-protein diet (6% protein) during pregnancy and lactation. The pups were kept with the mother throughout the lactation period (21 days), after which the offspring received a normal protein diet until 365 days of age. Histological (HE) and histoenzymological (NADH-TR) studies were conducted on the muscle fibers. The muscle was subjected to Nonspecific Esterase reaction to stain the Neuromuscular Junctions. Regarding the animals from the restricted group: the histologic analysis of the muscle fibers showed the presence of centralized nuclei and a diminished area; the histoenzymological study showed the different types of muscle fibers were randomly distributed in the EDL muscle and the area of the Type IIa muscle fiber was smaller; the ultrastructural study revealed disorganization of the Z line, and the presence of lipid droplets and vacuoles containing myelin figures in subsarcolemmal and intramiofibrilar regions; while the analysis of the NMJs exhibited no significant differences between the groups. Protein restriction in the pregnancy and lactation period may have affected the development of skeletal muscle, producing a permanent muscle-fiber deficit in the EDL muscle of the offspring. |
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Estudio morfológico y morfométrico de las fibras musculares y de las intersecciones neuromusculares del músculo extensor largo de los dedos de ratas de edad avanzada sometidas a la restricción proteica maternaMorphological and morphometric study of the muscle fibers and neuromuscular junctions of the extensor digitorum longus in aged rats submitted to maternal protein restrictionExtensor digitorum longus muscleMorphologyMorphometryProtein restrictionRatProtein restriction implies the functional involvement of several systems and organs, including the skeletal muscle, because it is a protein reservoir in the body. This study sought to analyze the morphological and morphometric features of the muscle fibers and neuromuscular junctions (NMJs) of the extensor digitorum longus (EDL) muscle in rats at 365 days of age, submitted to maternal protein restriction during the gestation and lactation periods. Wistar rats were divided into two groups: a Control Group mothers fed a normal-protein diet (17% protein) during pregnancy and lactation; and a Restricted Group - mothers fed a low-protein diet (6% protein) during pregnancy and lactation. The pups were kept with the mother throughout the lactation period (21 days), after which the offspring received a normal protein diet until 365 days of age. Histological (HE) and histoenzymological (NADH-TR) studies were conducted on the muscle fibers. The muscle was subjected to Nonspecific Esterase reaction to stain the Neuromuscular Junctions. Regarding the animals from the restricted group: the histologic analysis of the muscle fibers showed the presence of centralized nuclei and a diminished area; the histoenzymological study showed the different types of muscle fibers were randomly distributed in the EDL muscle and the area of the Type IIa muscle fiber was smaller; the ultrastructural study revealed disorganization of the Z line, and the presence of lipid droplets and vacuoles containing myelin figures in subsarcolemmal and intramiofibrilar regions; while the analysis of the NMJs exhibited no significant differences between the groups. Protein restriction in the pregnancy and lactation period may have affected the development of skeletal muscle, producing a permanent muscle-fiber deficit in the EDL muscle of the offspring.Biociências e Saúde Universidade Estadual do Oeste do ParanáCentro de Ciências Médicas e Farmacêuticas Universidade Estadual do Oeste do ParanáCentro de Ciências Biológicas e da Saúde Universidade Estadual do Oeste do ParanáDepartamento de Anatomia Instituto de Biociências Universidad Estatal PaulistaUniversidade Estadual do Oeste do ParanáUniversidad Estatal PaulistaJeronimo, L. C.Confortim, H. D.Centenaro, L. A.Brancalhão, R. M.C.Pinheiro, P. F.F.Matheus, S. M.M.Torrejais, M. M.2022-04-28T19:03:33Z2022-04-28T19:03:33Z2016-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article396-403http://dx.doi.org/10.4067/S0717-95022016000100057International Journal of Morphology, v. 34, n. 1, p. 396-403, 2016.0717-95020717-9367http://hdl.handle.net/11449/22061610.4067/S0717-950220160001000572-s2.0-84966501486Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Morphologyinfo:eu-repo/semantics/openAccess2022-04-28T19:03:33Zoai:repositorio.unesp.br:11449/220616Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:16:30.433652Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Estudio morfológico y morfométrico de las fibras musculares y de las intersecciones neuromusculares del músculo extensor largo de los dedos de ratas de edad avanzada sometidas a la restricción proteica materna Morphological and morphometric study of the muscle fibers and neuromuscular junctions of the extensor digitorum longus in aged rats submitted to maternal protein restriction |
title |
Estudio morfológico y morfométrico de las fibras musculares y de las intersecciones neuromusculares del músculo extensor largo de los dedos de ratas de edad avanzada sometidas a la restricción proteica materna |
spellingShingle |
Estudio morfológico y morfométrico de las fibras musculares y de las intersecciones neuromusculares del músculo extensor largo de los dedos de ratas de edad avanzada sometidas a la restricción proteica materna Jeronimo, L. C. Extensor digitorum longus muscle Morphology Morphometry Protein restriction Rat |
title_short |
Estudio morfológico y morfométrico de las fibras musculares y de las intersecciones neuromusculares del músculo extensor largo de los dedos de ratas de edad avanzada sometidas a la restricción proteica materna |
title_full |
Estudio morfológico y morfométrico de las fibras musculares y de las intersecciones neuromusculares del músculo extensor largo de los dedos de ratas de edad avanzada sometidas a la restricción proteica materna |
title_fullStr |
Estudio morfológico y morfométrico de las fibras musculares y de las intersecciones neuromusculares del músculo extensor largo de los dedos de ratas de edad avanzada sometidas a la restricción proteica materna |
title_full_unstemmed |
Estudio morfológico y morfométrico de las fibras musculares y de las intersecciones neuromusculares del músculo extensor largo de los dedos de ratas de edad avanzada sometidas a la restricción proteica materna |
title_sort |
Estudio morfológico y morfométrico de las fibras musculares y de las intersecciones neuromusculares del músculo extensor largo de los dedos de ratas de edad avanzada sometidas a la restricción proteica materna |
author |
Jeronimo, L. C. |
author_facet |
Jeronimo, L. C. Confortim, H. D. Centenaro, L. A. Brancalhão, R. M.C. Pinheiro, P. F.F. Matheus, S. M.M. Torrejais, M. M. |
author_role |
author |
author2 |
Confortim, H. D. Centenaro, L. A. Brancalhão, R. M.C. Pinheiro, P. F.F. Matheus, S. M.M. Torrejais, M. M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual do Oeste do Paraná Universidad Estatal Paulista |
dc.contributor.author.fl_str_mv |
Jeronimo, L. C. Confortim, H. D. Centenaro, L. A. Brancalhão, R. M.C. Pinheiro, P. F.F. Matheus, S. M.M. Torrejais, M. M. |
dc.subject.por.fl_str_mv |
Extensor digitorum longus muscle Morphology Morphometry Protein restriction Rat |
topic |
Extensor digitorum longus muscle Morphology Morphometry Protein restriction Rat |
description |
Protein restriction implies the functional involvement of several systems and organs, including the skeletal muscle, because it is a protein reservoir in the body. This study sought to analyze the morphological and morphometric features of the muscle fibers and neuromuscular junctions (NMJs) of the extensor digitorum longus (EDL) muscle in rats at 365 days of age, submitted to maternal protein restriction during the gestation and lactation periods. Wistar rats were divided into two groups: a Control Group mothers fed a normal-protein diet (17% protein) during pregnancy and lactation; and a Restricted Group - mothers fed a low-protein diet (6% protein) during pregnancy and lactation. The pups were kept with the mother throughout the lactation period (21 days), after which the offspring received a normal protein diet until 365 days of age. Histological (HE) and histoenzymological (NADH-TR) studies were conducted on the muscle fibers. The muscle was subjected to Nonspecific Esterase reaction to stain the Neuromuscular Junctions. Regarding the animals from the restricted group: the histologic analysis of the muscle fibers showed the presence of centralized nuclei and a diminished area; the histoenzymological study showed the different types of muscle fibers were randomly distributed in the EDL muscle and the area of the Type IIa muscle fiber was smaller; the ultrastructural study revealed disorganization of the Z line, and the presence of lipid droplets and vacuoles containing myelin figures in subsarcolemmal and intramiofibrilar regions; while the analysis of the NMJs exhibited no significant differences between the groups. Protein restriction in the pregnancy and lactation period may have affected the development of skeletal muscle, producing a permanent muscle-fiber deficit in the EDL muscle of the offspring. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-01 2022-04-28T19:03:33Z 2022-04-28T19:03:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4067/S0717-95022016000100057 International Journal of Morphology, v. 34, n. 1, p. 396-403, 2016. 0717-9502 0717-9367 http://hdl.handle.net/11449/220616 10.4067/S0717-95022016000100057 2-s2.0-84966501486 |
url |
http://dx.doi.org/10.4067/S0717-95022016000100057 http://hdl.handle.net/11449/220616 |
identifier_str_mv |
International Journal of Morphology, v. 34, n. 1, p. 396-403, 2016. 0717-9502 0717-9367 10.4067/S0717-95022016000100057 2-s2.0-84966501486 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Morphology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
396-403 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128492637782016 |