Scanning electron microscopy comparison of the resin–dentin interface using different specimen preparation methods
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jemt.23488 http://hdl.handle.net/11449/198788 |
Resumo: | Microscopy has been widely used to complement the data of studies related to dentin bonding; however, different specimen preparation methods may influence the analysis. Aiming to contribute to the reported scenario, this study evaluated the effect of two specimen-sectioning methods (cleavage and diamond disk cut) on the quality of the scanning electron microscopy (SEM) images. Four crowns of human molars were selected and had an area of approximately 6 mm2 of dentin exposed. They were then divided into two groups according to the universal adhesive application: total and self-etching modes. Then, composite blocks were built up and the specimens were stored in deionized water to allow the postcuring. The specimens were further divided according to the sectioning method: cleavage or diamond disk cut. Four specimens were obtained from each tooth. They were desiccated, placed on aluminum stubs, sputter-coated with gold, and observed in a scanning electron microscope, with ×2000 of magnification. The quality of the SEM images were evaluated by two calibrated examiners and classified into four scores (1–4). Mann–Whitney test (p <.05) showed that the diamond disk provided significantly higher scores than cleavage, whereas no significant difference was observed when comparing the total-etching and self-etching modes of application. The diamond disk cut method is preferable to the cleavage method to ensure the quality of the SEM analysis in studies involving the resin–dentin interface. |
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Scanning electron microscopy comparison of the resin–dentin interface using different specimen preparation methodsadhesive systembondingdentinscanning electron microscopyMicroscopy has been widely used to complement the data of studies related to dentin bonding; however, different specimen preparation methods may influence the analysis. Aiming to contribute to the reported scenario, this study evaluated the effect of two specimen-sectioning methods (cleavage and diamond disk cut) on the quality of the scanning electron microscopy (SEM) images. Four crowns of human molars were selected and had an area of approximately 6 mm2 of dentin exposed. They were then divided into two groups according to the universal adhesive application: total and self-etching modes. Then, composite blocks were built up and the specimens were stored in deionized water to allow the postcuring. The specimens were further divided according to the sectioning method: cleavage or diamond disk cut. Four specimens were obtained from each tooth. They were desiccated, placed on aluminum stubs, sputter-coated with gold, and observed in a scanning electron microscope, with ×2000 of magnification. The quality of the SEM images were evaluated by two calibrated examiners and classified into four scores (1–4). Mann–Whitney test (p <.05) showed that the diamond disk provided significantly higher scores than cleavage, whereas no significant difference was observed when comparing the total-etching and self-etching modes of application. The diamond disk cut method is preferable to the cleavage method to ensure the quality of the SEM analysis in studies involving the resin–dentin interface.Department of Restorative Dentistry Institute of Science and Technology São Paulo State University-UNESPDepartment of Prothodontics and Dental Materials Institute of Science and Technology São Paulo State University-UNESPDepartment of Restorative Dentistry Institute of Science and Technology São Paulo State University-UNESPDepartment of Prothodontics and Dental Materials Institute of Science and Technology São Paulo State University-UNESPUniversidade Estadual Paulista (Unesp)Augusto, Marina G. [UNESP]Dantas, Debora C. B. [UNESP]de Andrade, Guilherme S. [UNESP]Matuda, Amanda G. N. [UNESP]Lopes, Stephanie R. [UNESP]Barcellos, Daphne C. [UNESP]Pucci, Cesar R. [UNESP]2020-12-12T01:21:59Z2020-12-12T01:21:59Z2020-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article945-948http://dx.doi.org/10.1002/jemt.23488Microscopy Research and Technique, v. 83, n. 8, p. 945-948, 2020.1097-00291059-910Xhttp://hdl.handle.net/11449/19878810.1002/jemt.234882-s2.0-8508413926917540206528748500000-0003-4830-0400Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroscopy Research and Techniqueinfo:eu-repo/semantics/openAccess2021-10-23T17:46:00Zoai:repositorio.unesp.br:11449/198788Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Scanning electron microscopy comparison of the resin–dentin interface using different specimen preparation methods |
title |
Scanning electron microscopy comparison of the resin–dentin interface using different specimen preparation methods |
spellingShingle |
Scanning electron microscopy comparison of the resin–dentin interface using different specimen preparation methods Augusto, Marina G. [UNESP] adhesive system bonding dentin scanning electron microscopy |
title_short |
Scanning electron microscopy comparison of the resin–dentin interface using different specimen preparation methods |
title_full |
Scanning electron microscopy comparison of the resin–dentin interface using different specimen preparation methods |
title_fullStr |
Scanning electron microscopy comparison of the resin–dentin interface using different specimen preparation methods |
title_full_unstemmed |
Scanning electron microscopy comparison of the resin–dentin interface using different specimen preparation methods |
title_sort |
Scanning electron microscopy comparison of the resin–dentin interface using different specimen preparation methods |
author |
Augusto, Marina G. [UNESP] |
author_facet |
Augusto, Marina G. [UNESP] Dantas, Debora C. B. [UNESP] de Andrade, Guilherme S. [UNESP] Matuda, Amanda G. N. [UNESP] Lopes, Stephanie R. [UNESP] Barcellos, Daphne C. [UNESP] Pucci, Cesar R. [UNESP] |
author_role |
author |
author2 |
Dantas, Debora C. B. [UNESP] de Andrade, Guilherme S. [UNESP] Matuda, Amanda G. N. [UNESP] Lopes, Stephanie R. [UNESP] Barcellos, Daphne C. [UNESP] Pucci, Cesar R. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Augusto, Marina G. [UNESP] Dantas, Debora C. B. [UNESP] de Andrade, Guilherme S. [UNESP] Matuda, Amanda G. N. [UNESP] Lopes, Stephanie R. [UNESP] Barcellos, Daphne C. [UNESP] Pucci, Cesar R. [UNESP] |
dc.subject.por.fl_str_mv |
adhesive system bonding dentin scanning electron microscopy |
topic |
adhesive system bonding dentin scanning electron microscopy |
description |
Microscopy has been widely used to complement the data of studies related to dentin bonding; however, different specimen preparation methods may influence the analysis. Aiming to contribute to the reported scenario, this study evaluated the effect of two specimen-sectioning methods (cleavage and diamond disk cut) on the quality of the scanning electron microscopy (SEM) images. Four crowns of human molars were selected and had an area of approximately 6 mm2 of dentin exposed. They were then divided into two groups according to the universal adhesive application: total and self-etching modes. Then, composite blocks were built up and the specimens were stored in deionized water to allow the postcuring. The specimens were further divided according to the sectioning method: cleavage or diamond disk cut. Four specimens were obtained from each tooth. They were desiccated, placed on aluminum stubs, sputter-coated with gold, and observed in a scanning electron microscope, with ×2000 of magnification. The quality of the SEM images were evaluated by two calibrated examiners and classified into four scores (1–4). Mann–Whitney test (p <.05) showed that the diamond disk provided significantly higher scores than cleavage, whereas no significant difference was observed when comparing the total-etching and self-etching modes of application. The diamond disk cut method is preferable to the cleavage method to ensure the quality of the SEM analysis in studies involving the resin–dentin interface. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:21:59Z 2020-12-12T01:21:59Z 2020-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jemt.23488 Microscopy Research and Technique, v. 83, n. 8, p. 945-948, 2020. 1097-0029 1059-910X http://hdl.handle.net/11449/198788 10.1002/jemt.23488 2-s2.0-85084139269 1754020652874850 0000-0003-4830-0400 |
url |
http://dx.doi.org/10.1002/jemt.23488 http://hdl.handle.net/11449/198788 |
identifier_str_mv |
Microscopy Research and Technique, v. 83, n. 8, p. 945-948, 2020. 1097-0029 1059-910X 10.1002/jemt.23488 2-s2.0-85084139269 1754020652874850 0000-0003-4830-0400 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microscopy Research and Technique |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
945-948 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046708431552512 |