Uso de membrana reabsorvível de cortical bovina em defeitos críticos em calvária de ratos

• Autor(a) principal: Pires, Daniela de Oliveira [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/156999; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2018-08-09/000906401.pdf
• topic: Materiais biomédicos; Implantação dentária endo-óssea; Crânio; Ratos; Dental Implantation, Endosseous

Bone atrophy can result from etiologies as pathologies, trauma, surgical procedures and result of the physiological bone resorption following tooth loss. This fact has provided extensive studies on the process of bone repair. The objective of this study was to evaluate the efficacy of collagen absorbable membrane in critical defects in rat calvaria. Rattus norvegicus for that 36 were divided into control and treated groups. Animals were 8 mm diameter calvaria defects in their conducted specifically on the median sagittal suture in the frontal and parietal bones right and left. In the control group the defect was filled only by blood clot, while the treated group was filled with blood clot (GC), and covered with absorbable membrane (GCM) bovine collagen. The animals were euthanized at 7, 30 and 60 days postoperatively. The calvaria were removed and the excess material was removed, leaving only the cranial bones. The pieces were fixed in 10% formalin, followed by decalcification in EDTA. The obtained pieces were embedded in paraffin separately. After they were taken to the microtome, obtaining of 6μm thick sections and are mounted on slides. The blades aimed at quantitative analysis were stained with hematoxylin and eosin. To perform the analysis we used an optical microscope at 2.5x increase objective coupled to an image capture camera and connected to a computer with a software analyzer scanned images. The extent of bone formation in the defect area was measured. To this end, the use of some criteria was required. The total area of the defect in the analyzed region should match the area occupied by the bone of the calvaria in the same region before the defect production. For determination of the total area, should be set the height of the defect, having as parameter the thickness of bone at the defect edge. Once obtained the values of each blade, they are summed...